Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,978,727 T→C N58S (AAT→AGT)  lysA ← diaminopimelate decarboxylase, PLP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,978,7270TC99.3% 578.5 / NA 147N58S (AAT→AGT) lysAdiaminopimelate decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (74/72);  minor base G (0/1);  total (74/73)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

CGCCTGATCGATAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATATTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTGAGATCGGTAT  >  NC_000913/2978579‑2978874
                                                                                                                                                    |                                                                                                                                                   
  ccTGATCGATAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTgg                                                                                                                                                  >  3:532316/1‑150 (MQ=255)
  ccTGATCGATAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGa                                                                                                                                                 <  2:532337/151‑1 (MQ=255)
   cTGATCGATAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTgg                                                                                                                                                  <  4:723092/149‑1 (MQ=255)
      aTCGATAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAAc                                                                                                                                               >  1:1668186/1‑149 (MQ=255)
      aTCGATAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAg                                                                                                                                             <  4:601022/151‑1 (MQ=255)
       tCGATAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAgg                                                                                                                                            >  1:160510/1‑151 (MQ=255)
       tCGATAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAgg                                                                                                                                            >  4:626563/1‑151 (MQ=255)
       tCGATAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAg                                                                                                                                             <  1:559525/150‑1 (MQ=255)
         gATAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCt                                                                                                                                          >  4:679149/1‑151 (MQ=255)
          aTAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCtt                                                                                                                                         >  1:1663496/1‑151 (MQ=255)
            aaCATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCttt                                                                                                                                        <  4:130326/150‑1 (MQ=255)
            aaCATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTc                                                                                                                                       <  2:207728/151‑1 (MQ=255)
            aaCATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTc                                                                                                                                       >  2:321546/1‑151 (MQ=255)
              cATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCt                                                                                                                                      >  4:1852162/1‑150 (MQ=255)
               aTCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCtgt                                                                                                                                    >  1:1281195/1‑151 (MQ=255)
                 cTGCCGTAAAAACAATATCATCGGGGAGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGc                                                                                                                                  >  4:838689/1‑151 (MQ=255)
                   gCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCtgt                                                                                                                                    <  1:121636/147‑1 (MQ=255)
                   gCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCtgt                                                                                                                                    <  1:639800/147‑1 (MQ=255)
                   gCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCaa                                                                                                                                <  4:230969/151‑1 (MQ=255)
                   gCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCa                                                                                                                                 <  1:320709/150‑1 (MQ=255)
                    ccGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCaaa                                                                                                                               <  1:698251/151‑1 (MQ=255)
                    ccGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCaaa                                                                                                                               <  4:1687271/151‑1 (MQ=255)
                    ccGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCaaa                                                                                                                               <  4:112776/151‑1 (MQ=255)
                    ccGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCaa                                                                                                                                >  1:1189863/1‑150 (MQ=255)
                     cttAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCaa                                                                                                                                >  1:574495/3‑149 (MQ=255)
                       tAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAgcg                                                                                                                            >  1:1879874/1‑151 (MQ=255)
                        aaaaaCAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAgcgc                                                                                                                           >  1:907984/1‑151 (MQ=255)
                        aaaaaCAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAgcgc                                                                                                                           >  4:1551036/1‑151 (MQ=255)
                        aaaaaCAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAgcgc                                                                                                                           >  4:724642/1‑151 (MQ=255)
                           aaCAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGcacc                                                                                                                        >  4:1141273/1‑151 (MQ=255)
                            aCAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGcacca                                                                                                                       <  1:2066/151‑1 (MQ=255)
                              aaTATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACcaca                                                                                                                     <  1:650698/151‑1 (MQ=255)
                               atatCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACAt                                                                                                                    >  4:459660/1‑151 (MQ=255)
                                 atcatcGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATc                                                                                                                   <  4:889082/150‑1 (MQ=255)
                                 atcatcGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATc                                                                                                                   <  1:903573/150‑1 (MQ=255)
                                 atcatcGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCa                                                                                                                  <  1:1159953/151‑1 (MQ=255)
                                   catcGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCaa                                                                                                                 >  1:879805/1‑150 (MQ=255)
                                          gTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTc                                                                                                         <  2:468337/151‑1 (MQ=255)
                                           tGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTc                                                                                                         >  4:1693565/1‑150 (MQ=255)
                                           tGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTc                                                                                                         >  4:533057/1‑150 (MQ=255)
                                             cGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAgc                                                                                                      >  2:457261/1‑151 (MQ=255)
                                               tttGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAgct                                                                                                     <  4:316063/150‑2 (MQ=255)
                                                 tGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTg                                                                                                  >  3:391297/1‑151 (MQ=255)
                                                  gCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGc                                                                                                 >  4:1043981/1‑151 (MQ=255)
                                                    ggATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCa                                                                                                >  1:69333/1‑150 (MQ=255)
                                                    ggATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCa                                                                                                >  1:1804497/1‑150 (MQ=255)
                                                       ttGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCt                                                                                            >  4:58392/1‑151 (MQ=255)
                                                        tGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCt                                                                                            <  1:1275940/150‑1 (MQ=255)
                                                         gTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTg                                                                                           <  1:17030/150‑1 (MQ=255)
                                                         gTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGc                                                                                          <  1:293205/151‑1 (MQ=255)
                                                           aaCCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCcg                                                                                        <  1:1817606/151‑1 (MQ=255)
                                                            aCCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCcga                                                                                       <  2:369202/151‑1 (MQ=255)
                                                               cgccgcCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCcgacga                                                                                    <  4:1435525/151‑1 (MQ=255)
                                                               cgccgcCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCcgacg                                                                                     >  1:1137823/1‑150 (MQ=255)
                                                                gccgccAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCcgacga                                                                                    >  4:1328627/1‑150 (MQ=255)
                                                                  cgccACCGCACGCTCTACTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATACGAATACTGGAACAGGCTTTCTGCGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGaa                                                                                   >  1:491677/1‑149 (MQ=255)
                                                                  cgccAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGaat                                                                                  >  4:1873726/1‑150 (MQ=255)
                                                                      aaCGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAAttt                                                                             >  4:1556638/1‑151 (MQ=255)
                                                                      aaCGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAAttt                                                                             >  1:845572/1‑151 (MQ=255)
                                                                      aaCGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAAttt                                                                             <  4:1793529/151‑1 (MQ=255)
                                                                       acgcacgcTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTg                                                                            <  2:342600/151‑1 (MQ=255)
                                                                       acgcacgcTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTg                                                                            <  1:184548/151‑1 (MQ=255)
                                                                         gcacgcTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGTTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTgcg                                                                          <  4:1181388/151‑1 (MQ=255)
                                                                           acgcTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTgcgc                                                                         >  4:1888943/1‑150 (MQ=255)
                                                                           acgcTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCa                                                                        <  1:965398/151‑1 (MQ=255)
                                                                           acgcTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCa                                                                        <  4:1355329/151‑1 (MQ=255)
                                                                             gcTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATc                                                                      >  2:117221/1‑151 (MQ=255)
                                                                               tctATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGt                                                                    >  1:1716526/1‑151 (MQ=255)
                                                                                ctATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCg                                                                     <  4:577245/149‑1 (MQ=255)
                                                                                ctATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGt                                                                    >  4:1745322/1‑150 (MQ=255)
                                                                                  aTTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTa                                                                   <  3:321535/149‑1 (MQ=255)
                                                                                    ttCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGAcc                                                               >  4:873951/1‑151 (MQ=255)
                                                                                    ttCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGAc                                                                <  4:1507246/150‑1 (MQ=255)
                                                                                           aaCGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACAcc                                                         <  1:450839/150‑1 (MQ=255)
                                                                                           aaCGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCg                                                        <  4:1289674/151‑1 (MQ=255)
                                                                                             cGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCggg                                                      >  1:271948/1‑151 (MQ=255)
                                                                                             cGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCggg                                                      <  1:1200939/151‑1 (MQ=255)
                                                                                                 aCGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGc                                                  <  2:366098/151‑1 (MQ=255)
                                                                                                   ggAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCa                                                <  4:908377/151‑1 (MQ=255)
                                                                                                    gAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCaa                                               <  1:544246/151‑1 (MQ=255)
                                                                                                        ccACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAAtt                                            <  2:378101/150‑1 (MQ=255)
                                                                                                             ttCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGc                                        <  1:1463148/149‑1 (MQ=255)
                                                                                                               cACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGc                                    <  4:1190372/151‑1 (MQ=255)
                                                                                                                 cGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACTCCGGGCAGCCAAATTCAGCGGGCa                                   <  1:1242096/150‑1 (MQ=255)
                                                                                                                 cGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCaa                                  <  1:1361730/151‑1 (MQ=255)
                                                                                                                 cGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCa                                   <  2:88161/150‑1 (MQ=255)
                                                                                                                   cccTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAAc                                >  1:249365/1‑151 (MQ=255)
                                                                                                                     cTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACgc                              >  1:1397853/1‑151 (MQ=255)
                                                                                                                       gcgcgcGCATTGAGGGGAAAATGTGAATAGTGGAACAGGCTTTCTGTGGAAAGCGCACCACATGAAGCTGTTTCAGCGCTGCAATCTGCCGACGAGTAATTTGCGCAGCGTAGACCCGCGGCGGGCGGCGGAATTCGGGGGGCaagg                                <  1:431386/142‑3 (MQ=255)
                                                                                                                       gCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACgcag                            <  1:1817689/151‑1 (MQ=255)
                                                                                                                         tCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACgcagc                           >  1:1430785/1‑150 (MQ=255)
                                                                                                                                      gcAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGt             >  4:323314/1‑151 (MQ=255)
                                                                                                                                         aaaTATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTg            >  4:1033345/1‑149 (MQ=255)
                                                                                                                                           atatGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTg            >  2:185505/1‑147 (MQ=255)
                                                                                                                                           atatGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTGAGat        <  3:475253/151‑1 (MQ=255)
                                                                                                                                            tatGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTg            >  1:1605726/1‑146 (MQ=255)
                                                                                                                                             atGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTGAGatc       <  4:594353/150‑1 (MQ=255)
                                                                                                                                               gAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTGAGatcgg     <  1:1006991/150‑1 (MQ=255)
                                                                                                                                                aaTACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTGAGatcggta   <  1:176697/151‑1 (MQ=255)
                                                                                                                                                 atacTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTGAGatcggtat  >  4:1799177/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCCTGATCGATAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATATTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTGAGATCGGTAT  >  NC_000913/2978579‑2978874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: