BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,071,369	T→C	intergenic (‑125/+90)	fbaA ← / ← pgk	fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,071,369	0	T	C	100.0%	720.9 / NA	197	intergenic (‑125/+90)	fbaA/pgk	fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (105/92); total (105/92)

Only 100 of 199 total aligned reads displayed.

ACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACG  >  NC_000913/3071222‑3071516

aCGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTc                                                                                                                                                   <  4:1890205/150‑1 (MQ=255)

aCGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTc                                                                                                                                                   <  1:1875134/150‑1 (MQ=255)

    aaTCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGc                                                                                                                                               >  4:375908/1‑150 (MQ=255)

     aTCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCaa                                                                                                                                             <  1:1553990/151‑1 (MQ=255)

       cAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACt                                                                                                                                           >  1:909892/1‑151 (MQ=255)

        aaaaaTCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTc                                                                                                                                          >  4:740924/1‑151 (MQ=255)

        aaaaaTCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTc                                                                                                                                          <  4:241687/151‑1 (MQ=255)

        aaaaaTCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTc                                                                                                                                          >  1:1097668/1‑151 (MQ=255)

         aaaaTCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCg                                                                                                                                         >  4:1652034/1‑151 (MQ=255)

         aaaaTCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCg                                                                                                                                         <  2:123127/151‑1 (MQ=255)

         aaaaTCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCg                                                                                                                                         >  1:388900/1‑151 (MQ=255)

          aaaTCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCg                                                                                                                                         >  1:651626/1‑150 (MQ=255)

           aaTCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGaa                                                                                                                                       <  4:628368/151‑1 (MQ=255)

           aaTCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGa                                                                                                                                        >  4:874184/1‑150 (MQ=255)

             tCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAatt                                                                                                                                     >  1:156737/1‑151 (MQ=255)

                tAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAattatt                                                                                                                                  >  2:484301/1‑151 (MQ=255)

                tAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAattat                                                                                                                                   <  2:10802/150‑1 (MQ=255)

                tAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAatt                                                                                                                                     <  4:765101/148‑1 (MQ=255)

                tAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAatc                                                                                                                                     <  1:809696/148‑2 (MQ=255)

                 aGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTAttt                                                                                                                                 <  4:1151650/151‑1 (MQ=255)

                 aGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTAttt                                                                                                                                 >  4:1925607/1‑151 (MQ=255)

                 aGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTAttt                                                                                                                                 >  4:1077612/1‑151 (MQ=255)

                  gACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCAGTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTa                                                                                                                                <  1:172899/151‑1 (MQ=255)

                  gACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTAttt                                                                                                                                 >  1:984030/1‑150 (MQ=255)

                      tGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGt                                                                                                                            >  1:527878/1‑151 (MQ=255)

                       gTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGt                                                                                                                            <  4:1354140/150‑1 (MQ=255)

                       gTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGt                                                                                                                            <  4:67676/150‑1 (MQ=255)

                       gTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGt                                                                                                                            <  1:1439086/150‑1 (MQ=255)

                        tctcTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTc                                                                                                                                          <  2:509354/135‑1 (MQ=255)

                          tctTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATTTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATg                                                                                                                         >  3:406519/1‑150 (MQ=255)

                           ctTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTAt                                                                                                                          <  4:488170/148‑1 (MQ=255)

                           ctTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTAt                                                                                                                          <  1:754311/148‑1 (MQ=255)

                            ttGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGaa                                                                                                                       >  1:255016/1‑150 (MQ=255)

                             tGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGaaa                                                                                                                      <  4:1324274/150‑1 (MQ=255)

                              gTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGaaaaa                                                                                                                    <  1:164671/151‑1 (MQ=255)

                                  tGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGc                                                                                                                >  1:86386/1‑151 (MQ=255)

                                   gTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTg                                                                                                                 <  4:1662714/149‑1 (MQ=255)

                                      tcgtcgGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTg                                                                                                             <  3:232099/150‑1 (MQ=255)

                                      tcgtcgGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGc                                                                                                            >  4:149578/1‑151 (MQ=255)

                                          cgGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGctct                                                                                                         <  4:524773/150‑1 (MQ=255)

                                           gggCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTa                                                                                                        >  4:1545690/1‑150 (MQ=255)

                                            ggCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTaa                                                                                                       >  4:1025956/1‑150 (MQ=255)

                                                 tataCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAgcgc                                                                                                 >  4:1134957/1‑151 (MQ=255)

                                                  ataCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAgcgcg                                                                                                >  2:813/1‑151 (MQ=255)

                                                    aCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCt                                                                                              >  1:479956/1‑151 (MQ=255)

                                                      tCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTg                                                                                             >  1:767313/1‑150 (MQ=255)

                                                       cGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGaa                                                                                           <  1:1354133/151‑1 (MQ=255)

                                                            tACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACa                                                                                        >  1:21732/1‑149 (MQ=255)

                                                             aCTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAgg                                                                                     <  1:1428124/151‑1 (MQ=255)

                                                               ttCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAattatt                                                                                                                                  >  4:34609/1‑104 (MQ=17)

                                                               ttCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGc                                                                                   >  1:1165196/1‑151 (MQ=255)

                                                               ttCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGc                                                                                   >  1:624765/1‑151 (MQ=255)

                                                                tCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAggg                                                                                    <  4:932039/149‑1 (MQ=255)

                                                                 cAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAg                                                                                 <  1:725083/151‑1 (MQ=255)

                                                                   aGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAggg                                                                                    <  1:1651386/146‑1 (MQ=255)

                                                                   aGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGt                                                                               >  2:87948/1‑151 (MQ=255)

                                                                    gTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGtt                                                                              <  1:1793499/151‑1 (MQ=255)

                                                                     ttGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGttt                                                                             >  4:795675/1‑151 (MQ=255)

                                                                         aTGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTccct                                                                         >  1:634983/1‑151 (MQ=255)

                                                                         aTGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTccct                                                                         <  1:268165/151‑1 (MQ=255)

                                                                         aTGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTccct                                                                         <  4:549670/151‑1 (MQ=255)

                                                                           gtgCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTccctgc                                                                       >  1:128803/1‑151 (MQ=255)

                                                                               tgcgtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTccctgccctg                                                                   >  3:341937/1‑151 (MQ=255)

                                                                                gcgtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTccctgccct                                                                    <  3:91298/149‑1 (MQ=255)

                                                                                 cgtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCtgtg                                                                 <  1:1579481/151‑1 (MQ=255)

                                                                                  gtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCtgtg                                                                 >  1:502696/1‑150 (MQ=255)

                                                                                  gtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCtgtg                                                                 >  4:1339638/1‑150 (MQ=255)

                                                                                      gcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGAtttt                                                            >  1:302794/1‑151 (MQ=255)

                                                                                      gcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGAttt                                                             <  1:33777/150‑1 (MQ=255)

                                                                                       cgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGAttttt                                                           >  2:21180/1‑151 (MQ=255)

                                                                                        gtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGAtttttt                                                          >  4:1434183/1‑151 (MQ=255)

                                                                                        gtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGAtttttt                                                          >  4:1466848/1‑151 (MQ=255)

                                                                                        gtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGAtttttt                                                          >  4:85551/1‑151 (MQ=255)

                                                                                        gtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGAtttttt                                                          <  4:1649075/151‑1 (MQ=255)

                                                                                           cGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGTGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTAc                                                        <  3:459792/150‑1 (MQ=255)

                                                                                              tCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTActtct                                                    >  1:10473/1‑151 (MQ=255)

                                                                                               cACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTActtc                                                     <  4:1052767/149‑1 (MQ=255)

                                                                                                     aGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAgcgcg                                             >  1:346453/1‑151 (MQ=255)

                                                                                                         acttacttATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGctct                                         >  4:974659/1‑151 (MQ=255)

                                                                                                             acttaTGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCg                                      <  1:1271092/150‑1 (MQ=255)

                                                                                                             acttaTGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGa                                     <  1:873810/151‑1 (MQ=255)

                                                                                                              cttaTGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCg                                      >  4:1509183/1‑149 (MQ=255)

                                                                                                               ttaTGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGc                                   >  1:1007265/1‑151 (MQ=255)

                                                                                                                taTGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAg                                    <  2:528887/149‑1 (MQ=255)

                                                                                                                taTGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAg                                    <  4:1749334/149‑1 (MQ=255)

                                                                                                                 aTGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCAt                                 <  4:910286/151‑1 (MQ=255)

                                                                                                                   gTAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATc                                <  2:61071/150‑1 (MQ=255)

                                                                                                                    tAAGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGc                              >  1:806617/1‑151 (MQ=255)

                                                                                                                      aGCTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTa                            <  1:422760/151‑1 (MQ=255)

                                                                                                                        cTCCTGGGGATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTAc                           >  4:1281261/1‑150 (MQ=255)

                                                                                                                             ggggATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTgcag                      <  4:211544/150‑1 (MQ=255)

                                                                                                                              aggATTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTgcaggc                    >  4:1395104/2‑151 (MQ=255)

                                                                                                                                 aTTCACTCTCTTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGt                 <  1:1497635/151‑1 (MQ=255)

                                                                                                                                        ctctTGTCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTAc          >  3:204275/1‑151 (MQ=255)

                                                                                                                                              tCGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTcc     >  3:467797/1‑150 (MQ=255)

                                                                                                                                               cGCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCAc   >  4:444124/1‑151 (MQ=255)

                                                                                                                                                gCCCTCCTGCACCTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGCTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCAc   >  3:264865/1‑150 (MQ=255)

                                                                                                                                                gCCCTCCTGCAACTCGTATTATTTACAGTTTGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCTTGCCCTGTGATTTTTTACTTCTTCGCGCGCTCTTCGAGCATCGCTACTGCCGGCAGCACTTTAGCTTCCAc   >  3:88087/1‑150 (MQ=255)

                                                                                                                                                gCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACg  >  4:834225/1‑151 (MQ=255)

                                                                                                                                                gCCCTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACg  <  4:1118758/151‑1 (MQ=255)

ACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACG  >  NC_000913/3071222‑3071516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`