Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,086,618 A→G intergenic (+216/‑88) yqgC → / → metK uncharacterized protein/S‑adenosylmethionine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,086,6180AG99.3% 542.3 / ‑4.5 144intergenic (+216/‑88)yqgC/metKuncharacterized protein/S‑adenosylmethionine synthetase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (69/74);  total (70/74)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.96e-01

Only 100 of 144 total aligned reads displayed.

ATTGTCAGGAAAAATTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCT  >  NC_000913/3086471‑3086765
                                                                                                                                                   |                                                                                                                                                   
aTTGTCAGGAAAAATTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGAcc                                                                                                                                                  >  1:1913676/1‑151 (MQ=255)
 ttGTCAGGAAAAATTTCTAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCt                                                                                                                                                 >  1:418463/1‑151 (MQ=255)
  tGTCAGGAAAAATTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTg                                                                                                                                                >  1:1395104/1‑151 (MQ=255)
     cAGGAAAAATTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCa                                                                                                                                              <  1:1340341/150‑1 (MQ=255)
        gAAAAATTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGt                                                                                                                                          <  1:10431/151‑1 (MQ=255)
            aaTTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTg                                                                                                                                      >  1:1884953/1‑151 (MQ=255)
               ttCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGt                                                                                                                                    >  1:1563416/1‑150 (MQ=255)
                 ccAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAgg                                                                                                                                 >  1:849933/1‑151 (MQ=255)
                   aGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGAt                                                                                                                               <  4:574082/151‑1 (MQ=255)
                        cGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCt                                                                                                                          >  4:593151/1‑151 (MQ=255)
                        cGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCt                                                                                                                          >  2:517759/1‑151 (MQ=255)
                         gTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCt                                                                                                                          >  4:1345518/1‑150 (MQ=255)
                         gTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCt                                                                                                                          >  2:54102/1‑150 (MQ=255)
                           ttttAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTAc                                                                                                                        <  1:1032472/150‑1 (MQ=255)
                            tttAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTAcc                                                                                                                       >  4:353295/1‑150 (MQ=255)
                             ttAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACca                                                                                                                      <  1:945191/150‑1 (MQ=255)
                                  aGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGaaa                                                                                                                <  4:1233444/151‑1 (MQ=255)
                                   gAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGaaaa                                                                                                               <  4:1920969/151‑1 (MQ=255)
                                   gAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGaaaa                                                                                                               <  4:1469699/151‑1 (MQ=255)
                                     aTGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATc                                                                                                             <  2:305153/151‑1 (MQ=255)
                                        agaCACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCca                                                                                                           >  1:434307/1‑150 (MQ=255)
                                           cacGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCcacac                                                                                                        >  3:32831/1‑150 (MQ=255)
                                            acGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACacaa                                                                                                      <  4:1176670/151‑1 (MQ=255)
                                               aTTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACacaac                                                                                                     >  1:1408129/1‑149 (MQ=255)
                                               aTTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACacaac                                                                                                     >  1:135678/1‑149 (MQ=255)
                                                 tCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGt                                                                                                  <  2:431292/150‑1 (MQ=255)
                                                  cAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGttt                                                                                                <  4:1304701/151‑1 (MQ=255)
                                                  cAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGttt                                                                                                >  3:31892/1‑151 (MQ=255)
                                                  cAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGACATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGtt                                                                                                 <  1:1653074/150‑1 (MQ=255)
                                                   aaaaaaaaGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTg                                                                                               <  1:709054/151‑1 (MQ=255)
                                                     aaaaaaGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTg                                                                                               >  2:262414/1‑149 (MQ=255)
                                                      aaaaaGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGc                                                                                            >  3:530259/1‑151 (MQ=255)
                                                      aaaaaGAGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGc                                                                                            >  4:1081767/1‑151 (MQ=255)
                                                         aaGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTa                                                                                          >  4:1598376/1‑150 (MQ=255)
                                                             ggAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCaa                                                                                     <  4:1163633/151‑1 (MQ=255)
                                                             ggAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCaa                                                                                     <  4:714420/151‑1 (MQ=255)
                                                              gAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCaaa                                                                                    <  2:127099/151‑1 (MQ=255)
                                                                   aGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATtctc                                                                               >  1:1719728/1‑151 (MQ=255)
                                                                   aGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATtctc                                                                               >  2:177853/1‑151 (MQ=255)
                                                                   aGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATtct                                                                                <  1:365187/150‑1 (MQ=255)
                                                                   aGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATtct                                                                                <  1:1317857/150‑1 (MQ=255)
                                                                      gTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCttt                                                                            >  2:363196/1‑151 (MQ=255)
                                                                        gaagaaTTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAg                                                                          <  4:1665679/151‑1 (MQ=255)
                                                                        gaagaaTTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAg                                                                          <  2:394077/151‑1 (MQ=255)
                                                                         aagaaTTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAgg                                                                         <  1:58163/151‑1 (MQ=255)
                                                                           gaaTTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTg                                                                       <  1:1049706/151‑1 (MQ=255)
                                                                           gaaTTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTg                                                                       <  1:1703562/151‑1 (MQ=255)
                                                                             aTTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGat                                                                     >  2:179598/1‑151 (MQ=255)
                                                                             aTTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGat                                                                     <  1:562289/151‑1 (MQ=255)
                                                                             aTTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGat                                                                     <  1:46547/151‑1 (MQ=255)
                                                                               tGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGatat                                                                   >  1:585370/1‑151 (MQ=255)
                                                                                gACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATAtt                                                                  <  4:267786/151‑1 (MQ=255)
                                                                                gACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATAtt                                                                  <  1:1732329/151‑1 (MQ=255)
                                                                                 aCCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATAtt                                                                  >  1:31080/1‑150 (MQ=255)
                                                                                   cTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTaa                                                                >  1:1246454/1‑150 (MQ=255)
                                                                                    tAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAat                                                              >  4:178293/1‑151 (MQ=255)
                                                                                     aaaaTAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAat                                                              <  1:1518098/150‑1 (MQ=255)
                                                                                     aaaaTAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAat                                                              >  4:491557/1‑150 (MQ=255)
                                                                                       aaTAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATg                                                           <  4:53208/151‑1 (MQ=255)
                                                                                        aTAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATgg                                                          <  1:231888/151‑1 (MQ=255)
                                                                                         tAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGc                                                         >  2:513926/1‑151 (MQ=255)
                                                                                           gCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCaa                                                       <  4:1023841/151‑1 (MQ=255)
                                                                                            ccATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCaaa                                                      <  1:1819853/151‑1 (MQ=255)
                                                                                             cATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCaaa                                                      >  1:957697/1‑150 (MQ=255)
                                                                                             cATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCaaa                                                      >  3:475529/1‑150 (MQ=255)
                                                                                             cATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCaaa                                                      >  3:321646/1‑150 (MQ=255)
                                                                                              aTCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCaaaa                                                     >  4:1321662/1‑150 (MQ=255)
                                                                                               tCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAac                                                    >  3:126217/1‑150 (MQ=255)
                                                                                               tCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAac                                                    >  4:751894/1‑150 (MQ=255)
                                                                                                   gATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCtt                                               <  1:1347994/151‑1 (MQ=255)
                                                                                                      gTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCTAAACACCttttt                                            <  4:408042/151‑1 (MQ=255)
                                                                                                      gTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCttttt                                            >  3:244359/1‑151 (MQ=255)
                                                                                                      gTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCtttt                                             <  1:1493800/150‑1 (MQ=255)
                                                                                                       ttAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCttttt                                            >  4:1245536/1‑150 (MQ=255)
                                                                                                        tAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTa                                           <  1:130049/150‑1 (MQ=255)
                                                                                                            ccatccatACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTc                                       <  4:84965/150‑1 (MQ=255)
                                                                                                            ccatccatACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTc                                       <  1:1097391/150‑1 (MQ=255)
                                                                                                            ccatcaatACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTcc                                      >  1:78344/1‑151 (MQ=255)
                                                                                                              atccatACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCg                                     >  3:431019/1‑150 (MQ=255)
                                                                                                                ccatACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGt                                  <  4:815577/151‑1 (MQ=255)
                                                                                                                ccatACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGt                                  <  4:280736/151‑1 (MQ=255)
                                                                                                                ccatACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGt                                  <  4:56567/151‑1 (MQ=255)
                                                                                                                  atACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTcc                                <  2:520790/151‑1 (MQ=255)
                                                                                                                       gATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGtctc                           <  3:54102/151‑1 (MQ=255)
                                                                                                                       gATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGtctc                           >  3:389033/1‑151 (MQ=255)
                                                                                                                        aTTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTctct                          >  1:1545343/1‑151 (MQ=255)
                                                                                                                         ttAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTg                         >  1:502183/1‑151 (MQ=255)
                                                                                                                           aaCACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGaa                       <  4:1929178/151‑1 (MQ=255)
                                                                                                                           aaCACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGa                        <  4:1803272/150‑1 (MQ=255)
                                                                                                                                 cAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCa                  <  3:289730/150‑1 (MQ=255)
                                                                                                                                 cAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCAt                 >  4:863939/1‑151 (MQ=255)
                                                                                                                                   gACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATcc               >  4:478293/1‑151 (MQ=255)
                                                                                                                                    aCTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCt              <  1:1441844/151‑1 (MQ=255)
                                                                                                                                     cTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTg             >  1:154367/1‑151 (MQ=255)
                                                                                                                                      tGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTg             >  1:1835140/1‑150 (MQ=255)
                                                                                                                                         cAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACaa         <  4:1016809/151‑1 (MQ=255)
                                                                                                                                           gtgtTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACaaa        >  4:1073784/1‑150 (MQ=255)
                                                                                                                                              tttttGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTg    >  2:527134/1‑151 (MQ=255)
                                                                                                                                               ttttGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGc   >  1:859684/1‑151 (MQ=255)
                                                                                                                                                tttGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCt  >  1:1456838/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATTGTCAGGAAAAATTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCT  >  NC_000913/3086471‑3086765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: