Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,297,933 T→C Y69C (TAC→TGC)  yraQ ← putative inner membrane permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,297,9330TC100.0% 509.3 / NA 138Y69C (TAC→TGC) yraQputative inner membrane permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (79/59);  total (79/59)

Only 100 of 138 total aligned reads displayed.

AACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAA  >  NC_000913/3297786‑3298079
                                                                                                                                                   |                                                                                                                                                  
  cAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATcc                                                                                                                                               >  1:1133765/1‑151 (MQ=255)
   aGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATcc                                                                                                                                               >  1:1868305/1‑150 (MQ=255)
    gCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCCCCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCaa                                                                                                                                             <  3:355334/151‑1 (MQ=255)
     cGTCCCCACCGGCGTGCCGCGAAAGCGCGATTGCCCAGGCGTACGCAACAACCAGCCACGCGGGAGCAGCACGTGAATCAACAAACCGAGTTTCCCCCCAAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCa                                                                                                                                              <  3:313442/149‑1 (MQ=255)
      gTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTTCGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGACCCGAGGTTCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCaa                                                                                                                                             <  4:1874017/149‑1 (MQ=255)
        tCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAAcgc                                                                                                                                          <  4:1303218/150‑1 (MQ=255)
         cccAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGCCACGCGGGATCAGACCATGAATCAACGAACCGAGGATCCCCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAAcgcca                                                                                                                                        <  3:142145/151‑2 (MQ=255)
               agcGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGcc                                                                                                                                  >  4:874726/1‑151 (MQ=255)
                   tGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATgg                                                                                                                              >  4:864085/1‑151 (MQ=255)
                     ccGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGt                                                                                                                            >  1:1700226/1‑151 (MQ=255)
                        cgAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGtt                                                                                                                           >  1:723342/1‑149 (MQ=255)
                          aaaGCGCGATTGCCCAAGCGTACGCAACACCCAGTCACGCGGGATCAGCACCTGAATCACCGAACCGAGGATCCCCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTa                                                                                                                          <  4:301082/148‑1 (MQ=255)
                          aaaGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATGGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAg                                                                                                                         <  4:416263/149‑1 (MQ=255)
                          aaaGCCCGTTTGCCCAAGCGTACGCAACACCCAGTCCCGCGGGATCAGCACCTGAATCAACGAACGGAGGATCTCCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCa                                                                                                                       <  3:125301/151‑1 (MQ=255)
                           aaGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCAt                                                                                                                      >  1:1666546/1‑151 (MQ=255)
                            aGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATc                                                                                                                     >  1:1683832/1‑151 (MQ=255)
                             gcgcGATTGCCCAGGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCCCCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATcc                                                                                                                    <  1:1908493/151‑1 (MQ=255)
                             gcgcGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATcc                                                                                                                    >  2:510345/1‑151 (MQ=255)
                              cgcgATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCg                                                                                                                   >  1:1132236/1‑151 (MQ=255)
                                 gATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCt                                                                                                                >  4:407639/1‑151 (MQ=255)
                                  aTTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTg                                                                                                               >  3:46267/1‑151 (MQ=255)
                                    tGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTgcg                                                                                                             >  3:194704/1‑151 (MQ=255)
                                      cccAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTgcgc                                                                                                            >  2:83045/1‑150 (MQ=255)
                                      cccAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCa                                                                                                           <  1:1485129/151‑1 (MQ=255)
                                         aaGCGTACGCAACAACCAGCCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCCCCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAgg                                                                                                        <  4:1302109/151‑1 (MQ=255)
                                         aaGCGTACGAAACAACCAGTCACGGGGGATCAGCCCCTGAATCACCGACCCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAgg                                                                                                        <  4:391660/151‑1 (MQ=255)
                                          accttACGCACCACCCAGTCACGCGGGATCAGCCCCGGAATAAAGGATCCGAGGATCACCCCCAGCTCCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCaa                                                                                                          <  4:541998/144‑1 (MQ=255)
                                          aGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCAGCGCa                                                                                                           >  3:228422/1‑147 (MQ=255)
                                             gTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGAt                                                                                                      >  1:666950/1‑149 (MQ=255)
                                                cGCACCAACCAGCCACGCGGGATCAGCCCCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGAt                                                                                                  <  1:1813293/150‑1 (MQ=255)
                                                 gCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGAttt                                                                                                >  4:858831/1‑151 (MQ=255)
                                                  caacacCCAGTCACGCGGGATCAGCACCTGAATCAACGACCCGAGGATCCCCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGAtt                                                                                                 <  1:103395/149‑1 (MQ=255)
                                                  caacaaCCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTTCGCAAGGATAGAttt                                                                                                >  4:569156/1‑150 (MQ=255)
                                                   aacaacCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTAc                                                                                              >  1:49180/1‑151 (MQ=255)
                                                   aacaacCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTAc                                                                                              >  4:849379/1‑151 (MQ=255)
                                                     caacCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCg                                                                                            >  4:1064009/1‑151 (MQ=255)
                                                      acccAGTCACGCGGGATCAGCCCCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCg                                                                                            <  1:1747208/148‑1 (MQ=255)
                                                      aacCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCg                                                                                            >  2:199799/1‑150 (MQ=255)
                                                       acCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGa                                                                                           >  1:1460682/1‑150 (MQ=255)
                                                        ccAGTCACGCGGGATCAGCACCTGAATCACCGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATa                                                                                         <  4:509440/151‑1 (MQ=255)
                                                            gcACGCGGAATCAGCCCCGAAATCAACGAACCGAGGATCCCCCCAAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACtg                                                                                      <  3:67881/149‑1 (MQ=255)
                                                             cACGCGGGATCAGCCCCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACtgt                                                                                     <  2:505482/150‑1 (MQ=255)
                                                              aCGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTgg                                                                                   >  4:17547/1‑151 (MQ=255)
                                                               cgcgGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTggg                                                                                  >  3:27191/1‑151 (MQ=255)
                                                                  gggATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGttt                                                                               >  3:324143/1‑151 (MQ=255)
                                                                   ggATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTc                                                                              >  1:1224452/1‑151 (MQ=255)
                                                                    gATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCg                                                                             >  1:589428/1‑151 (MQ=255)
                                                                     aTCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCgg                                                                            >  1:528442/1‑151 (MQ=255)
                                                                     aTCAGCACCTGAATCAACGAAACGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCgg                                                                            >  1:1359411/1‑151 (MQ=255)
                                                                      tCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCgg                                                                            <  4:1257869/150‑1 (MQ=255)
                                                                        aGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGgca                                                                          <  2:229835/150‑1 (MQ=255)
                                                                         gCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGgcagc                                                                        >  4:1880633/1‑151 (MQ=255)
                                                                            ccgaaaccAACGAACCAAGGACCACCCCAATCTCCGCCGCTTCCCATCCCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGt                                                                     <  4:1195847/144‑1 (MQ=255)
                                                                             cTGAATCATAAAACCGAGGTGCACCCCCCCCCCCGCCGCGTTCCTTGCCGGGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTTCCATGGGGTGGCATCCGCCTGCGCAAGGATAGATTTTCCGATACTGTGGGGTTCGgcag                                                                         <  1:1625593/146‑1 (MQ=255)
                                                                              tGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTa                                                                    >  2:391830/1‑150 (MQ=255)
                                                                                aaTCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTaaa                                                                  <  4:1775603/150‑1 (MQ=255)
                                                                                    aaCGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCtt                                                             <  4:1305989/151‑1 (MQ=255)
                                                                                         aCCAAGGACCCCCCCCACCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTg                                                           <  3:275951/148‑1 (MQ=255)
                                                                                            gAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCgt                                                       >  1:282627/1‑149 (MQ=255)
                                                                                            gAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCgt                                                       >  2:24970/1‑149 (MQ=255)
                                                                                             aGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCATGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCAgtag                                                     >  4:23391/1‑150 (MQ=255)
                                                                                                 tCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAgg                                                >  1:1002788/1‑151 (MQ=255)
                                                                                                 tCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAgg                                                >  4:1824047/1‑151 (MQ=255)
                                                                                                  cccccccATCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAg                                                 <  4:34375/147‑1 (MQ=255)
                                                                                                  cACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAgg                                                <  4:551434/150‑1 (MQ=255)
                                                                                                       ccAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCa                                          <  4:1374718/151‑1 (MQ=255)
                                                                                                         aGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCAtt                                        >  1:1105576/1‑151 (MQ=255)
                                                                                                         aGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCAtt                                        <  2:119905/151‑1 (MQ=255)
                                                                                                             ccgccgCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTcac                                    <  3:16929/151‑1 (MQ=255)
                                                                                                             ccgccgCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTca                                     <  4:867050/150‑1 (MQ=255)
                                                                                                             ccgccgCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTc                                      <  3:401587/149‑1 (MQ=255)
                                                                                                              cgccgcTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTcac                                    >  1:678308/1‑150 (MQ=255)
                                                                                                               gccgcTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACAt                                  <  4:587073/151‑1 (MQ=255)
                                                                                                                  gcTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATAcc                               >  4:619664/1‑151 (MQ=255)
                                                                                                                  gcTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATAcc                               <  1:874352/151‑1 (MQ=255)
                                                                                                                    tttCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATAcc                               >  4:798178/1‑149 (MQ=255)
                                                                                                                    tttCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATAcc                               >  3:20487/1‑149 (MQ=255)
                                                                                                                      tCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCag                             >  4:1084388/1‑149 (MQ=255)
                                                                                                                       ccATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCag                             >  1:103016/1‑148 (MQ=255)
                                                                                                                       ccATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCag                             >  1:1195187/1‑148 (MQ=255)
                                                                                                                        cATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGAc                         >  1:1728871/1‑151 (MQ=255)
                                                                                                                           aCCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCg                       <  1:878309/150‑1 (MQ=255)
                                                                                                                             cgcgAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCg                       >  4:413005/1‑148 (MQ=255)
                                                                                                                              gcgAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCa                    >  1:498420/1‑150 (MQ=255)
                                                                                                                              gcgAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCa                    >  1:937469/1‑150 (MQ=255)
                                                                                                                               cgAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAAt                  <  2:392995/151‑1 (MQ=255)
                                                                                                                                 aGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATg                 >  1:1788718/1‑150 (MQ=255)
                                                                                                                                 aGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATg                 >  3:49998/1‑150 (MQ=255)
                                                                                                                                  ggAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGGCCGGCAATGa                <  1:1661316/150‑1 (MQ=255)
                                                                                                                                  ggAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGAc               >  1:1179860/1‑151 (MQ=255)
                                                                                                                                    aaaTAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACaa             <  1:918719/151‑1 (MQ=255)
                                                                                                                                    aaaTAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACaa             <  3:452035/151‑1 (MQ=255)
                                                                                                                                     aaTAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAAc            >  1:1615474/1‑151 (MQ=255)
                                                                                                                                        aGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACgag         <  1:544944/151‑1 (MQ=255)
                                                                                                                                        aGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACg           >  4:1531235/1‑149 (MQ=255)
                                                                                                                                        aGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACg           >  1:1373605/1‑149 (MQ=255)
                                                                                                                                          atcatcGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCTATAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACgaga        >  4:665801/1‑150 (MQ=255)
                                                                                                                                          atcatcGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACgaga        >  4:1221103/1‑150 (MQ=255)
                                                                                                                                               cgcgCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAgaaagaaa  <  1:1558880/151‑1 (MQ=255)
                                                                                                                                               cgcgCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAgaaagaaa  <  4:422518/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAA  >  NC_000913/3297786‑3298079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑