Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,319,794 T→C R386R (CGT→CGC argG → argininosuccinate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,319,7940TC99.3% 502.7 / NA 135R386R (CGT→CGCargGargininosuccinate synthetase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (66/68);  total (67/68)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 136 total aligned reads displayed.

TTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGCTTCAGGC  >  NC_000913/3319646‑3319941
                                                                                                                                                    |                                                                                                                                                   
ttGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCt                                                                                                                                                   >  1:1061510/1‑151 (MQ=255)
ttGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCt                                                                                                                                                   >  3:48171/1‑151 (MQ=255)
 tGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTg                                                                                                                                                  >  1:1774468/1‑151 (MQ=255)
  gACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTg                                                                                                                                                  >  1:7726/1‑150 (MQ=255)
  gACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTg                                                                                                                                                  <  4:790448/150‑1 (MQ=255)
       ccAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATg                                                                                                                                            <  3:34938/151‑1 (MQ=255)
        cAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATgg                                                                                                                                           <  2:145862/151‑1 (MQ=255)
        cAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATgg                                                                                                                                           <  1:547923/151‑1 (MQ=255)
         aGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGa                                                                                                                                          <  4:872060/151‑1 (MQ=255)
            cgcTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGaaa                                                                                                                                        >  4:78605/1‑150 (MQ=255)
            cgcTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGaaa                                                                                                                                        <  3:494545/150‑1 (MQ=255)
                    ctgcGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACt                                                                                                                               >  1:578137/1‑151 (MQ=255)
                    ctgcGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACt                                                                                                                               >  1:1229156/1‑151 (MQ=255)
                      gcGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTc                                                                                                                              <  1:345670/150‑1 (MQ=255)
                       cGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCgg                                                                                                                            >  4:1294946/1‑151 (MQ=255)
                        gTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGgt                                                                                                                           >  2:176965/1‑151 (MQ=255)
                         tGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGgtg                                                                                                                          >  1:721016/1‑151 (MQ=255)
                          gACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGgtg                                                                                                                          <  3:415212/150‑1 (MQ=255)
                             tctctGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTtct                                                                                                                      >  1:715997/1‑151 (MQ=255)
                                  gCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTtct                                                                                                                      <  2:208380/146‑1 (MQ=255)
                                  gCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGcc                                                                                                                  >  2:6130/1‑150 (MQ=255)
                                    aaCGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGcc                                                                                                                  >  1:1275894/1‑148 (MQ=255)
                                    aaCGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGcc                                                                                                                  >  4:413945/1‑148 (MQ=255)
                                     aCGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTAACGATGGAAAAAGGCGCCTCGGTGTTCTCGCCAgat                                                                                                              <  1:88719/151‑1 (MQ=255)
                                       gCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAgatg                                                                                                             >  2:115076/1‑150 (MQ=255)
                                        cTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAgatgat                                                                                                           >  4:1174702/1‑151 (MQ=255)
                                           ggTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCg                                                                                                         >  3:30912/1‑150 (MQ=255)
                                           ggTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGt                                                                                                        >  1:806938/1‑151 (MQ=255)
                                           ggTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGt                                                                                                        <  1:1028911/151‑1 (MQ=255)
                                            gTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTa                                                                                                       <  3:144862/151‑1 (MQ=255)
                                            gTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTa                                                                                                       <  4:961235/151‑1 (MQ=255)
                                             ttGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTa                                                                                                       >  3:121201/1‑150 (MQ=255)
                                               gccagccaGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTAtt                                                                                                     <  1:1402539/150‑1 (MQ=255)
                                               gccagccaGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTg                                                                                                    <  2:491401/151‑1 (MQ=255)
                                               gccagccaGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTg                                                                                                    <  1:82792/151‑1 (MQ=255)
                                                ccagccagATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTgg                                                                                                   <  3:169448/151‑1 (MQ=255)
                                                 cagccagATCTCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGt                                                                                                  <  4:1572139/151‑1 (MQ=255)
                                                     cagATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAAt                                                                                              <  1:9620/151‑1 (MQ=255)
                                                       gATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTg                                                                                            <  2:302042/151‑1 (MQ=255)
                                                        aTCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTg                                                                                            >  4:295890/1‑150 (MQ=255)
                                                        aTCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGa                                                                                           <  1:503881/151‑1 (MQ=255)
                                                        aTCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGa                                                                                           <  4:1151457/151‑1 (MQ=255)
                                                            cTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCAt                                                                                       >  1:1844391/1‑151 (MQ=255)
                                                              ggTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGc                                                                                     <  1:956898/151‑1 (MQ=255)
                                                               gTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCg                                                                                    <  4:973166/151‑1 (MQ=255)
                                                                       aCCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTgg                                                                            >  1:1749778/1‑151 (MQ=255)
                                                                        cccTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTgg                                                                            >  1:1747701/1‑150 (MQ=255)
                                                                         ccTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGat                                                                          <  4:1539245/151‑1 (MQ=255)
                                                                         ccTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGat                                                                          <  4:1601345/151‑1 (MQ=255)
                                                                         ccTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGat                                                                          <  1:169478/151‑1 (MQ=255)
                                                                          cTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGat                                                                          >  4:905949/1‑150 (MQ=255)
                                                                           tGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGatat                                                                        >  1:1074296/1‑151 (MQ=255)
                                                                           tGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGatat                                                                        >  4:731532/1‑151 (MQ=255)
                                                                            ggAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGatat                                                                        <  4:293155/150‑1 (MQ=255)
                                                                              aGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCAc                                                                     <  2:531389/151‑1 (MQ=255)
                                                                              aGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCAc                                                                     <  1:1782932/151‑1 (MQ=255)
                                                                                cTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTg                                                                   >  1:504606/1‑151 (MQ=255)
                                                                                 tGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTg                                                                   >  4:458246/1‑150 (MQ=255)
                                                                                  gcgcCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGCTGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTg                                                                   <  2:461140/149‑1 (MQ=255)
                                                                                    gcCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATAc                                                               >  1:1205358/1‑151 (MQ=255)
                                                                                    gcCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATAc                                                               >  1:41583/1‑151 (MQ=255)
                                                                                      cGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATAccc                                                             >  4:1082569/1‑151 (MQ=255)
                                                                                      cGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCACGCGTAACCTGGATATCACTGATAccc                                                             >  3:538189/1‑151 (MQ=255)
                                                                                            aaCGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCcgcg                                                          >  1:200183/1‑148 (MQ=255)
                                                                                             aCGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGaa                                                      <  3:374624/151‑1 (MQ=255)
                                                                                              cGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGaa                                                      >  4:29334/1‑150 (MQ=255)
                                                                                                   attCAATCCTGAATACCGTCTCAGGGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCACGCGTAACCTGGATATCACTGATACCCGCGAGAAACttt                                                 <  3:7385/150‑1 (MQ=255)
                                                                                                    ttCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACtttt                                                <  4:1878891/150‑1 (MQ=255)
                                                                                                      cAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTTGATATCACTGATACCCGCGAGAAACTTTTCg                                              <  2:63101/150‑1 (MQ=255)
                                                                                                      cAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCgg                                             >  1:1864078/1‑151 (MQ=255)
                                                                                                         tCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGtt                                           >  1:540763/1‑150 (MQ=255)
                                                                                                          ccTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTAt                                         <  4:346446/151‑1 (MQ=255)
                                                                                                          ccTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTAt                                         >  2:49813/1‑151 (MQ=255)
                                                                                                          ccTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTAt                                         <  4:1874061/151‑1 (MQ=255)
                                                                                                             gAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATg                                        <  1:1659625/149‑1 (MQ=255)
                                                                                                                tACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCCGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCaa                                    >  1:1554940/1‑150 (MQ=255)
                                                                                                                 aCCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCaaaa                                  <  4:757706/151‑1 (MQ=255)
                                                                                                                  ccGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAAc                                 <  4:716311/151‑1 (MQ=255)
                                                                                                                  ccGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAAc                                 <  4:795522/151‑1 (MQ=255)
                                                                                                                    gTCTCAGAGAACCTGGCCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAAc                                 <  2:219010/149‑1 (MQ=255)
                                                                                                                    gTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTg                               >  1:1468725/1‑151 (MQ=255)
                                                                                                                    gTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTg                               <  1:895716/151‑1 (MQ=255)
                                                                                                                      ctcAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGCATAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGc                             >  3:414904/1‑151 (MQ=255)
                                                                                                                       tcAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGcc                            >  1:1791608/1‑151 (MQ=255)
                                                                                                                       gcAGAGAACATGACCGAGGAGGGGGAGAGTGGGAGGATGAAAAGAGGCGACTCGGGGTGCCCGCCAGATTATAAAAAAGGTCAATAAACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAAATTTTCGGTTATGCAAAAACTGGcc                            >  1:1866826/2‑151 (MQ=255)
                                                                                                                        cAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGcc                            <  1:560401/150‑1 (MQ=255)
                                                                                                                        cAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGc                             <  4:900590/149‑1 (MQ=255)
                                                                                                                         agagAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTg                          >  1:806723/1‑151 (MQ=255)
                                                                                                                          gagaACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCGAAAACTgg                              <  3:463955/146‑1 (MQ=255)
                                                                                                                             aaCCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCttt                      >  1:954010/1‑151 (MQ=255)
                                                                                                                               ccTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTtcc                    <  4:537424/151‑1 (MQ=255)
                                                                                                                                 tGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTtcctc                  >  1:1443622/1‑151 (MQ=255)
                                                                                                                                     cTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCctct              >  2:324901/1‑151 (MQ=255)
                                                                                                                                         aaGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCg          >  2:145011/1‑151 (MQ=255)
                                                                                                                                          aGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGc         <  1:285459/151‑1 (MQ=255)
                                                                                                                                            ccAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGCtt       >  4:581327/1‑151 (MQ=255)
                                                                                                                                             cAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGCTTc      <  4:1845110/151‑1 (MQ=255)
                                                                                                                                              agagCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGCTTCa     <  4:1469314/151‑1 (MQ=255)
                                                                                                                                              agagCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGCTTCa     <  4:1205872/151‑1 (MQ=255)
                                                                                                                                                agCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGCTTCAgg   <  1:270475/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGCTTCAGGC  >  NC_000913/3319646‑3319941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: