Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,507,100 T→C L79L (TTA→TTG)  yhfT ← inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,507,1000TC99.2% 453.3 / ‑4.4 124L79L (TTA→TTG) yhfTinner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (64/59);  total (65/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 124 total aligned reads displayed.

CCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCC  >  NC_000913/3506954‑3507246
                                                                                                                                                  |                                                                                                                                                  
ccTAATACATCCACCGCCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGTCTGTTTATCGCCAGAACGCCGCGGATATCGGAAGGAAGAAAGAGCAAc                                                                                                                                                 >  1:1086576/1‑150 (MQ=255)
 cTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCaa                                                                                                                                                  >  1:848017/1‑148 (MQ=255)
  tAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCAt                                                                                                                                              >  2:438289/1‑151 (MQ=255)
      aCATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGt                                                                                                                                          <  1:1509916/151‑1 (MQ=255)
       cATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGtt                                                                                                                                         <  4:905744/151‑1 (MQ=255)
       cATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGtt                                                                                                                                         <  1:712058/151‑1 (MQ=255)
        aTCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTg                                                                                                                                        <  1:1096855/151‑1 (MQ=255)
        aTCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTg                                                                                                                                        <  2:494193/151‑1 (MQ=255)
          ccACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGa                                                                                                                                       <  3:34390/150‑1 (MQ=255)
           cACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGagc                                                                                                                                     <  2:202885/151‑1 (MQ=255)
            aCCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGagc                                                                                                                                     >  4:1919195/1‑150 (MQ=255)
             ccGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGagcag                                                                                                                                   <  4:1317089/151‑1 (MQ=255)
             ccGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGagcag                                                                                                                                   <  2:265521/151‑1 (MQ=255)
             ccGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGagc                                                                                                                                     >  1:280681/1‑149 (MQ=255)
              cGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGagcagc                                                                                                                                  >  4:241691/1‑151 (MQ=255)
               ggCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGcc                                                                                                                                 >  4:738669/1‑151 (MQ=255)
               ggCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGcc                                                                                                                                 <  2:303026/151‑1 (MQ=255)
                 cAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCg                                                                                                                               >  4:1714995/1‑151 (MQ=255)
                  aGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCgg                                                                                                                              >  4:1052301/1‑151 (MQ=255)
                   gcgcGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCgg                                                                                                                              >  4:1167962/1‑150 (MQ=255)
                   gcgcGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGt                                                                                                                             <  1:1119504/151‑1 (MQ=255)
                   gcgcGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGt                                                                                                                             >  1:419541/1‑151 (MQ=255)
                    cgcgGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGtt                                                                                                                            <  4:968226/151‑1 (MQ=255)
                     gcgGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGttt                                                                                                                           <  1:1586721/151‑1 (MQ=255)
                      cgGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGtttt                                                                                                                          <  1:1150928/151‑1 (MQ=255)
                       ggTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGtttt                                                                                                                          <  2:532876/150‑1 (MQ=255)
                       ggTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGttt                                                                                                                           <  4:261257/149‑1 (MQ=255)
                       ggTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTc                                                                                                                         <  4:856262/151‑1 (MQ=255)
                         tCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTc                                                                                                                         >  1:633892/1‑149 (MQ=255)
                               gcTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTaaa                                                                                                                 <  4:1316393/151‑1 (MQ=255)
                                     ttACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAag                                                                                                               >  4:1662391/1‑147 (MQ=255)
                                      tACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAag                                                                                                               >  4:1387378/1‑146 (MQ=255)
                                       aCTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATcc                                                                                                         >  1:1411543/1‑151 (MQ=255)
                                          ggCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATccc                                                                                                        <  4:1451131/149‑1 (MQ=255)
                                           gCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCAc                                                                                                     <  2:513124/151‑1 (MQ=255)
                                            cAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCAcc                                                                                                    <  2:280321/151‑1 (MQ=255)
                                            cAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCAcc                                                                                                    <  3:315180/151‑1 (MQ=255)
                                            cAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCAcc                                                                                                    >  1:1460438/1‑151 (MQ=255)
                                             aaCAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCg                                                                                                   <  4:356916/151‑1 (MQ=255)
                                              aCAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGa                                                                                                  <  4:1805443/151‑1 (MQ=255)
                                               cAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCg                                                                                                   >  1:522195/1‑149 (MQ=255)
                                                aGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAgg                                                                                                >  3:148064/1‑151 (MQ=255)
                                                  gCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGcc                                                                                              <  4:393542/151‑1 (MQ=255)
                                                   cAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGcc                                                                                              >  3:22686/1‑150 (MQ=255)
                                                   cAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGcc                                                                                              >  3:386960/1‑150 (MQ=255)
                                                      gTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACaa                                                                                          <  4:1305446/151‑1 (MQ=255)
                                                       tAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACaa                                                                                          >  4:1006053/1‑150 (MQ=255)
                                                        aaGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACaaa                                                                                         >  4:64057/1‑150 (MQ=255)
                                                           gATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAAcc                                                                                      >  1:1286232/1‑150 (MQ=255)
                                                           gATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGGAAAAGAGATCCCCACCGAGGCCACAAAAcc                                                                                      >  3:380479/1‑150 (MQ=255)
                                                              cAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAAt                                                                                   <  3:384635/150‑1 (MQ=255)
                                                               aaCACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGc                                                                                 >  4:773647/1‑151 (MQ=255)
                                                                acacGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCt                                                                                >  1:697760/1‑151 (MQ=255)
                                                                         aGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCaa                                                                        >  1:1769456/1‑150 (MQ=255)
                                                                          gATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCaaaa                                                                      <  4:213963/151‑1 (MQ=255)
                                                                             aGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGc                                                                    <  4:1742412/150‑1 (MQ=255)
                                                                             aGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCg                                                                   >  1:1342309/1‑151 (MQ=255)
                                                                             aGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCg                                                                   >  1:1784885/1‑151 (MQ=255)
                                                                               cgcCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGAt                                                                 >  1:1396193/1‑151 (MQ=255)
                                                                               cgcCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGAt                                                                 <  1:11771/151‑1 (MQ=255)
                                                                                   aaGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTc                                                             >  3:27466/1‑151 (MQ=255)
                                                                                    aGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTcc                                                            >  4:899120/1‑151 (MQ=255)
                                                                                        cAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGc                                                        >  4:1479714/1‑151 (MQ=255)
                                                                                         aaaCGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGcc                                                       <  3:543954/151‑1 (MQ=255)
                                                                                         aaaCGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGcc                                                       >  4:1015872/1‑151 (MQ=255)
                                                                                          aaCGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCt                                                      <  1:1302164/151‑1 (MQ=255)
                                                                                          aaCGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCt                                                      <  4:452229/151‑1 (MQ=255)
                                                                                                  ccggCTGTTTATCGCCAGGGCGCCGAGAATAGCGGGAGGAAAAAAGAGCAGCCATGCGTGGAGCAGCCCGGTTTTCAGGGTAAAAGCGACCCCCCCCGAGCCCAAAAAACAAACGCCCAAACCAAAGGCGATACTCCCCGCCTcg                                                    <  1:453483/143‑1 (MQ=255)
                                                                                                  cAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACgg                                              <  4:1061073/151‑1 (MQ=255)
                                                                                                    ggCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGtt                                            <  1:1035646/151‑1 (MQ=255)
                                                                                                    ggCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGtt                                            <  3:78214/151‑1 (MQ=255)
                                                                                                         tttATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCata                                        <  2:125072/150‑1 (MQ=255)
                                                                                                          ttATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCatat                                       >  1:649050/1‑150 (MQ=255)
                                                                                                           tATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCAtata                                      <  1:901128/150‑1 (MQ=255)
                                                                                                               gCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCtt                                 <  2:116108/151‑1 (MQ=255)
                                                                                                                 cAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTc                                <  3:342124/150‑1 (MQ=255)
                                                                                                                 cAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTc                                >  1:1552393/1‑150 (MQ=255)
                                                                                                                 cAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCa                               <  1:386130/151‑1 (MQ=255)
                                                                                                                  aGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCa                               >  4:566405/1‑150 (MQ=255)
                                                                                                                   ggACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCaa                              <  1:1412052/150‑1 (MQ=255)
                                                                                                                    gACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGGAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATc                            >  1:481556/1‑151 (MQ=255)
                                                                                                                      cGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCa                           <  1:758197/150‑1 (MQ=255)
                                                                                                                       gCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGt                         <  3:139900/151‑1 (MQ=255)
                                                                                                                        ccGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGtt                        >  1:868025/1‑151 (MQ=255)
                                                                                                                         cGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTg                       >  1:285973/1‑151 (MQ=255)
                                                                                                                          gAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTg                       <  3:95237/150‑1 (MQ=255)
                                                                                                                           aGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCg                     <  4:177203/151‑1 (MQ=255)
                                                                                                                                atCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAgg                >  1:33984/1‑151 (MQ=255)
                                                                                                                                  cGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGa               >  2:119908/1‑150 (MQ=255)
                                                                                                                                  cGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGAt              >  3:482602/1‑151 (MQ=255)
                                                                                                                                   gatAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGACTTCAATCAGTTgagcca                  >  3:31797/3‑141 (MQ=255)
                                                                                                                                    gTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCCCCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCg            <  1:356818/151‑1 (MQ=255)
                                                                                                                                      aGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAATCCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCg            <  1:1540968/149‑1 (MQ=255)
                                                                                                                                      aGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCggg          >  1:865930/1‑151 (MQ=255)
                                                                                                                                        gaagaaAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCg        >  4:829953/1‑151 (MQ=255)
                                                                                                                                         aagaaagaGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCgg       >  3:152415/1‑151 (MQ=255)
                                                                                                                                          agaaagaGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCgg       >  1:1682727/1‑150 (MQ=255)
                                                                                                                                          agaaagaGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGa      <  4:1627654/151‑1 (MQ=255)
                                                                                                                                              agagCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGcc  <  1:167263/151‑1 (MQ=255)
                                                                                                                                              agagCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGcc  >  4:1310742/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCC  >  NC_000913/3506954‑3507246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑