Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,589,630 A→G V133A (GTG→GCG)  ugpE ← sn‑glycerol‑3‑phosphate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,589,6300AG100.0% 532.8 / NA 149V133A (GTG→GCG) ugpEsn‑glycerol‑3‑phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (57/92);  total (57/92)

Only 100 of 150 total aligned reads displayed.

GCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCACCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAA  >  NC_000913/3589483‑3589775
                                                                                                                                                   |                                                                                                                                                 
gcgcCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTccgccg                                                                                                                                                <  4:1496354/151‑1 (MQ=255)
    cGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCgg                                                                                                                                            >  1:1595643/1‑151 (MQ=255)
       cGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGaa                                                                                                                                         <  4:790294/151‑1 (MQ=255)
       cGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGCTGGCGATGACTTCCGCCGTCGGGa                                                                                                                                          >  1:1225146/1‑150 (MQ=255)
        gATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAATAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAg                                                                                                                                        <  2:26193/151‑1 (MQ=255)
        gATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAg                                                                                                                                        <  1:1703593/151‑1 (MQ=255)
           ccGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGAt                                                                                                                                      <  4:1457247/150‑1 (MQ=255)
                 ggCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACt                                                                                                                               >  2:448753/1‑151 (MQ=255)
                 ggCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACt                                                                                                                               <  4:1010156/151‑1 (MQ=255)
                     tCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCa                                                                                                                            <  2:386664/150‑1 (MQ=255)
                     tCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCa                                                                                                                            <  4:10748/150‑1 (MQ=255)
                      ccaccaGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCaa                                                                                                                           <  4:796124/150‑1 (MQ=255)
                       caccaGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAAcc                                                                                                                         <  1:181173/151‑1 (MQ=255)
                        accaGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCg                                                                                                                        >  4:1161201/1‑151 (MQ=255)
                           aGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGgca                                                                                                                     <  4:86353/151‑1 (MQ=255)
                            gCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGgc                                                                                                                      >  4:1293435/1‑149 (MQ=255)
                              tCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGgcagc                                                                                                                   >  1:533142/1‑150 (MQ=255)
                               cATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCAt                                                                                                                 <  1:289874/151‑1 (MQ=255)
                               cATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCAt                                                                                                                 >  4:30915/1‑151 (MQ=255)
                                aTCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGCCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATc                                                                                                                >  2:134456/1‑151 (MQ=255)
                                  ccGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCa                                                                                                               <  1:228982/150‑1 (MQ=255)
                                  ccGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAg                                                                                                              <  1:90596/151‑1 (MQ=255)
                                    ggCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAggg                                                                                                            <  2:216454/151‑1 (MQ=255)
                                    ggCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAggg                                                                                                            >  1:1766688/1‑151 (MQ=255)
                                    ggCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAggg                                                                                                            <  3:320456/151‑1 (MQ=255)
                                    ggCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAggg                                                                                                            >  3:504006/1‑151 (MQ=255)
                                     gCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAggg                                                                                                            >  1:1833942/1‑150 (MQ=255)
                                      cAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTg                                                                                                          <  1:1352565/151‑1 (MQ=255)
                                       aGCGTCATAAAGAACTGGCGGCACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGACCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACCTCAACCGGCAGCATCAg                                                                                                              >  3:495321/1‑146 (MQ=255)
                                       aGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGa                                                                                                         <  4:575322/151‑1 (MQ=255)
                                        gCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGAt                                                                                                        <  4:6358/151‑1 (MQ=255)
                                          gTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATaa                                                                                                      <  1:684177/151‑1 (MQ=255)
                                          gTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATa                                                                                                       >  3:55583/1‑150 (MQ=255)
                                          gTCATAAAGAACTGGCGGAACAGGAAAGTACCGGTCGTCGAGAACATCAGCGGCAGCGTTAAACCGGCGTAGCTGACGAGCATCTGCTGGCTGCCGATGCCTTCCGCCGTCGGGCAGATACGGACTTCAACCGGCAGCATGAgggag                                                                                                          >  4:1429842/1‑145 (MQ=255)
                                            cATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATaaaa                                                                                                    <  3:287631/151‑1 (MQ=255)
                                            cATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATaaa                                                                                                     <  1:290488/150‑1 (MQ=255)
                                             aTAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCGTCAGCGGCAGCGTTAAACCGGCGTATCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATa                                                                                                       <  4:1076743/147‑1 (MQ=255)
                                               aaaGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAatc                                                                                                 >  4:1232323/1‑151 (MQ=255)
                                                   aaCTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATcc                                                                                             >  4:1794042/1‑151 (MQ=255)
                                                    aCTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCa                                                                                            <  2:114820/151‑1 (MQ=255)
                                                       ggCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCagaa                                                                                         <  4:212971/151‑1 (MQ=255)
                                                        gCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCagaag                                                                                        <  2:359105/151‑1 (MQ=255)
                                                          ggAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCagaagaa                                                                                      <  3:414907/151‑1 (MQ=255)
                                                             accggAAAGTAGCGGTCGCCGAGGCCATCATCGGCCGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCATGGTTATAAAAATCATCCAGAAGAagag                                                                                   <  1:143676/148‑1 (MQ=255)
                                                              cAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAgg                                                                                  <  4:595370/151‑1 (MQ=255)
                                                               aGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGt                                                                                 >  1:1121623/1‑151 (MQ=255)
                                                               aGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGt                                                                                 >  4:1278695/1‑151 (MQ=255)
                                                                 gAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTa                                                                               <  1:1170863/151‑1 (MQ=255)
                                                                  aaaGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTAc                                                                              <  1:1101684/151‑1 (MQ=255)
                                                                     gTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGt                                                                            >  4:1312/1‑150 (MQ=255)
                                                                     gTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTa                                                                           <  1:456875/151‑1 (MQ=255)
                                                                     gTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTa                                                                           <  4:329624/151‑1 (MQ=255)
                                                                           gTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGaa                                                                     <  1:803921/151‑1 (MQ=255)
                                                                             cGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGaaaa                                                                   <  4:319871/151‑1 (MQ=255)
                                                                               ccGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACg                                                                 >  2:183691/1‑151 (MQ=255)
                                                                                cGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGa                                                                <  1:353581/151‑1 (MQ=255)
                                                                                 gAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGaa                                                               <  3:21478/151‑1 (MQ=255)
                                                                                  aGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGaaa                                                              <  3:133291/151‑1 (MQ=255)
                                                                                  aGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGaa                                                               >  4:887113/1‑150 (MQ=255)
                                                                                  aGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGaa                                                               >  2:364992/1‑150 (MQ=255)
                                                                                    gCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAAcc                                                            >  4:1089710/1‑151 (MQ=255)
                                                                                      cATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAg                                                          <  1:23152/151‑1 (MQ=255)
                                                                                      cATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAg                                                          <  3:183684/151‑1 (MQ=255)
                                                                                      cATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAg                                                          <  2:350646/151‑1 (MQ=255)
                                                                                       aTCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAAcc                                                            >  1:561261/1‑148 (MQ=255)
                                                                                       aTCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGa                                                         <  1:804753/151‑1 (MQ=255)
                                                                                         cAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGAc                                                        >  4:1757335/1‑150 (MQ=255)
                                                                                         cAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACa                                                       <  3:201717/151‑1 (MQ=255)
                                                                                             ggCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATgg                                                   <  4:419864/151‑1 (MQ=255)
                                                                                                      aaaCCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCg                                          >  1:63135/1‑151 (MQ=255)
                                                                                                      aaaCCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCg                                          <  2:178686/151‑1 (MQ=255)
                                                                                                        aCCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCg                                          >  1:416459/1‑149 (MQ=255)
                                                                                                        aCCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCg                                          >  1:920665/1‑149 (MQ=255)
                                                                                                          cGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGagag                                      <  1:1013156/151‑1 (MQ=255)
                                                                                                           ggCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGTCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCgag                                        >  1:634839/1‑148 (MQ=255)
                                                                                                            gCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCa                                    <  4:377498/151‑1 (MQ=255)
                                                                                                             cGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCAt                                   >  1:98166/1‑151 (MQ=255)
                                                                                                             cGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCAt                                   <  4:1892869/151‑1 (MQ=255)
                                                                                                              gTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATc                                  <  1:390839/151‑1 (MQ=255)
                                                                                                              gTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATc                                  >  4:1721811/1‑151 (MQ=255)
                                                                                                                aGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCg                                 <  3:475475/150‑1 (MQ=255)
                                                                                                                aGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCg                                 <  1:68227/150‑1 (MQ=255)
                                                                                                                  cTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCg                                 >  1:1299241/1‑148 (MQ=255)
                                                                                                                  cTGTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCg                                 >  1:1850691/1‑148 (MQ=255)
                                                                                                                    gTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCgaga                              <  4:1767381/149‑1 (MQ=255)
                                                                                                                    gTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGAc                             >  4:1728320/1‑150 (MQ=255)
                                                                                                                    gTCGAGCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGAc                             <  1:496469/150‑1 (MQ=255)
                                                                                                                     tCGAGAATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGCCgg                           >  1:1589221/1‑151 (MQ=255)
                                                                                                                         gCATCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAAt                       <  1:136480/151‑1 (MQ=255)
                                                                                                                           aTCTGCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAAttt                     >  4:638693/1‑151 (MQ=255)
                                                                                                                               gCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTg                   <  4:1557392/149‑1 (MQ=255)
                                                                                                                               gCAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGc                  <  4:908112/150‑1 (MQ=255)
                                                                                                                                cAGGTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCg                <  2:544489/151‑1 (MQ=255)
                                                                                                                                   gTTGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGc             <  4:749126/151‑1 (MQ=255)
                                                                                                                                    ttGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCg            >  4:1268653/1‑151 (MQ=255)
                                                                                                                                     tGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCg            <  4:1325628/150‑1 (MQ=255)
                                                                                                                                     tGGCGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGt           >  2:220520/1‑151 (MQ=255)
                                                                                                                                        cGATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAAt        <  1:413987/151‑1 (MQ=255)
                                                                                                                                         gATGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATg       <  1:952100/151‑1 (MQ=255)
                                                                                                                                          aTGACTTCCGCCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGc      <  3:157013/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCGTAGCTGTCGAGCATCTGCAGGTTGGCGATGACTTCCACCGTCGGGAAGATACGTACTTCAACCGGCAGCATCAGGGTGATAAAAATCATCCAGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAA  >  NC_000913/3589483‑3589775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑