Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,707,870 T→C intergenic (‑165/+746) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,707,8700TC99.6% 998.6 / ‑4.4 276intergenic (‑165/+746)dppA/proKdipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (148/127);  total (148/128)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 276 total aligned reads displayed.

GATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACC  >  NC_000913/3707723‑3708013
                                                                                                                                                   |                                                                                                                                               
      tgttgtTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACa                                                                                                                                         >  3:508089/1‑150 (MQ=255)
          gttTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTg                                                                                                                                    >  3:436682/1‑151 (MQ=255)
           ttttAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGt                                                                                                                                   >  4:98701/1‑151 (MQ=255)
           ttttAACCCTTTGCAGTGGGTTGTCGCTGCCAGACATTTTTTGTTTTTTGCCAGGGCGGGTGAACGTTATGCGGTGGGGATGCCGTACTAATTAACATCACTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAAttt                                                                                                                                     >  1:1645949/1‑147 (MQ=255)
            tttAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTc                                                                                                                                  >  4:307916/1‑151 (MQ=255)
            tttAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTc                                                                                                                                  >  4:165877/1‑151 (MQ=255)
            tttAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTc                                                                                                                                  >  4:1130266/1‑151 (MQ=255)
             ttAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTc                                                                                                                                  >  1:67334/1‑150 (MQ=255)
              tAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATACAATGCCCCTCGCGACCTACAATCTGTCa                                                                                                                                 >  1:1366118/1‑150 (MQ=255)
              tAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCa                                                                                                                                 <  1:1847880/150‑1 (MQ=255)
               aaCCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCaa                                                                                                                                >  3:149402/1‑150 (MQ=255)
                 cccTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAAc                                                                                                                               >  4:1883088/1‑149 (MQ=255)
                  ccTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAAc                                                                                                                               >  4:668658/1‑148 (MQ=255)
                   cTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGa                                                                                                                            >  4:647995/1‑150 (MQ=255)
                   cTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGa                                                                                                                            >  4:1157481/1‑150 (MQ=255)
                     ttGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGaa                                                                                                                           <  1:1144262/149‑1 (MQ=255)
                     ttGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGaa                                                                                                                           <  1:47000/149‑1 (MQ=255)
                      tGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAAtgt                                                                                                                        >  3:364579/1‑151 (MQ=255)
                         aGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGa                                                                                                                      >  3:198274/1‑150 (MQ=255)
                             ggTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACg                                                                                                                 >  1:631791/1‑151 (MQ=255)
                                tGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTc                                                                                                               <  1:1256951/150‑1 (MQ=255)
                                  tCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCATGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCa                                                                                                              <  1:892565/149‑1 (MQ=255)
                                   cGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAAt                                                                                                            >  4:750412/1‑150 (MQ=255)
                                      tGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATAc                                                                                                          >  2:277556/1‑149 (MQ=255)
                                       gCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGc                                                                                                       >  1:1358082/1‑151 (MQ=255)
                                        ccTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGcc                                                                                                      >  4:1463598/1‑151 (MQ=255)
                                         cTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGcc                                                                                                      >  4:491845/1‑150 (MQ=255)
                                           gACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCaa                                                                                                    <  4:1877019/150‑1 (MQ=255)
                                           gACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCa                                                                                                     <  1:1046075/149‑1 (MQ=255)
                                            aCCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAAcc                                                                                                  >  1:108404/1‑151 (MQ=255)
                                             ccTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGt                                                                                                                <  2:151650/136‑1 (MQ=255)
                                             ccTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCg                                                                                                 <  4:1390863/151‑1 (MQ=255)
                                             ccTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCg                                                                                                 >  4:1520542/1‑151 (MQ=255)
                                              cTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCgg                                                                                                >  4:982727/1‑151 (MQ=255)
                                               ttttttgttttttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCggg                                                                                               >  4:519827/1‑151 (MQ=255)
                                                 ttttgttttttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGa                                                                                              <  1:1495857/150‑1 (MQ=255)
                                                       tttttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTacac                                                                                       >  1:449696/1‑151 (MQ=255)
                                                       tttttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTacac                                                                                       >  4:1148035/1‑151 (MQ=255)
                                                        ttttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACAcc                                                                                      >  1:523231/1‑151 (MQ=255)
                                                          ttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCa                                                                                     <  1:1551288/150‑1 (MQ=255)
                                                          ttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCa                                                                                     >  3:157153/1‑150 (MQ=255)
                                                           tgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAAc                                                                                   >  4:533479/1‑151 (MQ=255)
                                                            gCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACg                                                                                  >  1:828166/1‑151 (MQ=255)
                                                                ggTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTga                                                                              <  4:1294028/151‑1 (MQ=255)
                                                                ggTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTga                                                                              <  4:950511/151‑1 (MQ=255)
                                                                   cGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTgaga                                                                            >  4:1185021/1‑150 (MQ=255)
                                                                   cGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTgaga                                                                            >  4:251842/1‑150 (MQ=255)
                                                                      gTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACCCCAACGGTGAGAATcc                                                                        <  1:1392924/151‑1 (MQ=255)
                                                                       tCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATcc                                                                        >  2:395344/1‑150 (MQ=255)
                                                                          aCGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCcacac                                                                    >  3:333729/1‑151 (MQ=255)
                                                                              tATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCcacacaca                                                                 >  1:1340985/1‑150 (MQ=255)
                                                                              tATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCcacacaca                                                                 <  4:1054129/150‑1 (MQ=255)
                                                                                 gAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAg                                                              <  1:802655/150‑1 (MQ=255)
                                                                                 gAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGa                                                             <  4:1324480/151‑1 (MQ=255)
                                                                                  aGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTCACACCAACGGTGAGAATCCACACACAAAGAt                                                            <  4:325833/151‑1 (MQ=255)
                                                                                   ggTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGAtt                                                           <  1:978405/151‑1 (MQ=255)
                                                                                   ggTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGAtt                                                           <  3:71587/151‑1 (MQ=255)
                                                                                   ggTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGAt                                                            <  1:938835/150‑1 (MQ=255)
                                                                                    gTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTa                                                          <  2:122581/151‑1 (MQ=255)
                                                                                        ggATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTaaaaa                                                      <  1:987026/151‑1 (MQ=255)
                                                                                              cGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTc                                                <  1:1794387/151‑1 (MQ=255)
                                                                                              cGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTc                                                >  4:1175244/1‑151 (MQ=255)
                                                                                               gTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCa                                               <  4:1626877/151‑1 (MQ=255)
                                                                                                 aCTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCaa                                              <  1:921857/150‑1 (MQ=255)
                                                                                                  cTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAAc                                            >  1:1773426/1‑151 (MQ=255)
                                                                                                    aaTTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAAc                                            <  4:1393019/149‑1 (MQ=255)
                                                                                                    aaTTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACa                                           <  2:501507/150‑1 (MQ=255)
                                                                                                     aTTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGc                                         <  4:1018695/151‑1 (MQ=255)
                                                                                                     aTTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGc                                         <  1:328626/151‑1 (MQ=255)
                                                                                                      ttAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCt                                        >  1:1919106/1‑151 (MQ=255)
                                                                                                        aaCATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTAt                                      <  1:1434329/151‑1 (MQ=255)
                                                                                                          cATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTAttt                                    >  1:1163957/1‑151 (MQ=255)
                                                                                                           aTCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTg                                   <  2:230688/151‑1 (MQ=255)
                                                                                                           aTCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTg                                   >  1:1199529/1‑151 (MQ=255)
                                                                                                             cAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTgca                                 <  4:1835831/151‑1 (MQ=255)
                                                                                                             cAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTgc                                  >  4:832381/1‑150 (MQ=255)
                                                                                                              aGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTgc                                  >  4:856016/1‑149 (MQ=255)
                                                                                                                   aaCAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGcagcag                             >  4:579399/1‑149 (MQ=255)
                                                                                                                   aaCAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCaga                            <  2:193746/150‑1 (MQ=255)
                                                                                                                   aaCAGTGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCaga                            <  3:260316/150‑1 (MQ=255)
                                                                                                                        tGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAAtt                      >  3:486403/1‑151 (MQ=255)
                                                                                                                        tGCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAAtt                      >  4:736032/1‑151 (MQ=255)
                                                                                                                         gCGGATCCTCCATAAAATGCCCCTCGCGACCTTCAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAAtt                      <  4:1830264/150‑1 (MQ=255)
                                                                                                                         gCGGATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAAttt                     <  1:745433/151‑1 (MQ=255)
                                                                                                                           ggATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTtgt                   >  1:57074/1‑151 (MQ=255)
                                                                                                                           ggATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTtgt                   <  4:148886/151‑1 (MQ=255)
                                                                                                                           ggATCCTCCATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTtgt                   <  2:67753/151‑1 (MQ=255)
                                                                                                                               cctccATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGca                <  1:1300303/150‑2 (MQ=255)
                                                                                                                                 tccATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACt             >  4:1690358/1‑151 (MQ=255)
                                                                                                                                   cATAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTcc           <  1:1660860/151‑1 (MQ=255)
                                                                                                                                    aTAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCa          <  1:263326/151‑1 (MQ=255)
                                                                                                                                     tAAAATGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCa          >  1:1685413/1‑150 (MQ=255)
                                                                                                                                      aaaaTGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCaa         <  1:1870524/150‑1 (MQ=255)
                                                                                                                                        aaTGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACAt      <  2:325288/151‑1 (MQ=255)
                                                                                                                                        aaTGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACAt      <  4:208554/151‑1 (MQ=255)
                                                                                                                                          tGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATg     >  4:1226145/1‑150 (MQ=255)
                                                                                                                                          tGCCCCTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATg     >  1:1478688/1‑150 (MQ=255)
                                                                                                                                            ccccTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGAcc  >  2:449052/1‑151 (MQ=255)
                                                                                                                                              ccTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGAcc  >  4:1779061/1‑149 (MQ=255)
                                                                                                                                              ccTCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGAcc  >  1:771643/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACC  >  NC_000913/3707723‑3708013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: