BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,891,768	G→A	A52T (GCA→ACA)	mdtL →	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,891,768	0	G	A	100.0%	560.1 / NA	143	A52T (GCA→ACA)	mdtL	multidrug efflux system protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (72/71); total (72/71)

Only 100 of 143 total aligned reads displayed.

CCGCTTTTTGATTTGTAGTTTTGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGGCAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTAC  >  NC_000913/3891620‑3891915

   cTTTTTGATTTGTAGTTTTGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCt                                                                                                                                                >  4:417508/1‑151 (MQ=255)

     ttttGATTTGTAGTTTTGCCCTGGTTTTCCTTTAGCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTg                                                                                                                                               <  1:1740859/150‑1 (MQ=255)

     ttttGATTTGTAGTTTTGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCt                                                                                                                                                <  4:399806/149‑1 (MQ=255)

     ttttGATTTGTAGTTTTGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCt                                                                                                                                                <  1:973990/149‑1 (MQ=255)

     ttttGATTTGTAGTTTTGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTg                                                                                                                                               >  4:706154/1‑150 (MQ=255)

     ttttGATTTGTAGTTTTGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGc                                                                                                                                              >  4:714015/1‑151 (MQ=255)

     ttttGATTTGTAGTTTTGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGc                                                                                                                                              >  3:46008/1‑151 (MQ=255)

      tttGATTTGTAGTTTTGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCg                                                                                                                                             >  2:367161/1‑151 (MQ=255)

      tttGATTTGTAGTTTTGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCg                                                                                                                                             >  4:1169902/1‑151 (MQ=255)

       ttGATTTGTAGTTTTGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCg                                                                                                                                             <  4:169338/150‑1 (MQ=255)

        tGATTTGTAGTTTTGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACTGCTGCg                                                                                                                                             >  1:1883092/1‑149 (MQ=255)

        tGATTTGTAGTTTTGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGAt                                                                                                                                           >  1:1579323/1‑151 (MQ=255)

          aTTTGTAGTTTTGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGt                                                                                                                                         <  4:543591/151‑1 (MQ=255)

            ttGTAGTTTTGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGtt                                                                                                                                        <  4:489443/150‑1 (MQ=255)

             tGTAGTTTTGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTa                                                                                                                                       <  2:353291/150‑1 (MQ=255)

             tGTAGTTTTGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTAt                                                                                                                                      >  1:1575610/1‑151 (MQ=255)

              gTAGTTTTGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTAt                                                                                                                                      <  2:157542/150‑1 (MQ=255)

               tAGTTTTGCCTTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTAtt                                                                                                                                     <  1:1621579/150‑1 (MQ=255)

                aGTTTTGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTg                                                                                                                                   >  1:1222195/1‑151 (MQ=255)

                  ttttGCCCTGGTTTTCCTTTACCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTg                                                                                                                                   <  1:1081085/149‑1 (MQ=255)

                  ttttGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGc                                                                                                                                  <  1:1548098/150‑1 (MQ=255)

                   tttGCCCTGGTTTTCCTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGcc                                                                                                                                 <  3:211010/150‑1 (MQ=255)

                     tGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCgg                                                                                                                               >  1:1643491/1‑150 (MQ=255)

                        ccTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTaaa                                                                                                                           <  2:70407/151‑1 (MQ=255)

                         cTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTaa                                                                                                                            >  1:271017/1‑149 (MQ=255)

                         cTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTaa                                                                                                                            >  4:1552082/1‑149 (MQ=255)

                          tGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGt                                                                                                                         >  4:920816/1‑151 (MQ=255)

                           ggTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTg                                                                                                                        >  4:1627057/1‑151 (MQ=255)

                                 aCTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGa                                                                                                                   <  4:1644283/150‑1 (MQ=255)

                                 aCTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGAt                                                                                                                  >  4:265754/1‑151 (MQ=255)

                                   tttATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCg                                                                                                                >  1:1266379/1‑151 (MQ=255)

                                    ttATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATc                                                                                                                 <  3:440375/149‑1 (MQ=255)

                                    ttATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGt                                                                                                               >  4:1401291/1‑151 (MQ=255)

                                         ccgccgGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAgg                                                                                                          <  1:175932/151‑1 (MQ=255)

                                         ccgccgGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAgg                                                                                                          >  3:96826/1‑151 (MQ=255)

                                          cgccgGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCTGTAAAGTGGCCGATCGTTCAggg                                                                                                         <  1:1410672/151‑1 (MQ=255)

                                           gccgGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAggg                                                                                                         >  4:447531/1‑150 (MQ=255)

                                             cgGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAggg                                                                                                         >  4:1491185/1‑148 (MQ=255)

                                              gggATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGaa                                                                                                     <  4:819549/151‑1 (MQ=255)

                                               ggATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGaaa                                                                                                    <  3:273553/151‑1 (MQ=255)

                                                gATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGaa                                                                                                     >  4:1353025/1‑149 (MQ=255)

                                                    gATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCgg                                                                                               <  4:1368850/151‑1 (MQ=255)

                                                     atatCTACCTCGTTGGTTTACCGCGAATCGCCGCCGATCTCATTGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGATATTAGCCGGTAAAGTGGCCGATCGTTCCGGGAGAATGCCTGt                                                                                              >  1:1824518/1‑151 (MQ=255)

                                                      tatGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGt                                                                                              <  1:1047696/150‑1 (MQ=255)

                                                       atGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTc                                                                                             <  1:1341599/150‑1 (MQ=255)

                                                       atGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCg                                                                                            >  3:232096/1‑151 (MQ=255)

                                                        tGTACCTCGTTGGTTTACCGGGCACCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCGCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTc                                                                                             <  1:1529884/149‑1 (MQ=255)

                                                            ccTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCAt                                                                                        <  4:1303064/150‑1 (MQ=255)

                                                                gTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCcg                                                                                   >  4:188487/1‑151 (MQ=255)

                                                                gTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCcg                                                                                   >  1:1682537/1‑151 (MQ=255)

                                                                  tGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCcgg                                                                                  <  4:775541/150‑1 (MQ=255)

                                                                   ggTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCcggcg                                                                                >  1:323115/1‑151 (MQ=255)

                                                                   ggTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCcggc                                                                                 <  3:499291/150‑1 (MQ=255)

                                                                    gTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCcggcgc                                                                               >  4:723443/1‑151 (MQ=255)

                                                                      ttACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCcggcgcgg                                                                             >  2:422381/1‑151 (MQ=255)

                                                                         ccGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCcggcgcggcg                                                                           <  1:555468/150‑1 (MQ=255)

                                                                          cgcgCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCt                                                                         >  4:782664/1‑151 (MQ=255)

                                                                           gcgcATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCta                                                                        <  1:155584/151‑1 (MQ=255)

                                                                            cgcATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCta                                                                        <  1:1730388/150‑1 (MQ=255)

                                                                               aTCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCtattt                                                                     >  4:118515/1‑150 (MQ=255)

                                                                               aTCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCtattt                                                                     >  2:18740/1‑150 (MQ=255)

                                                                                  gccgccGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCtatttatt                                                                  >  4:893818/1‑150 (MQ=255)

                                                                                   ccgccgATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTtattat                                                                >  2:245032/1‑151 (MQ=255)

                                                                                   ccgccgATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTtattat                                                                <  2:401441/151‑1 (MQ=255)

                                                                                    cgccgATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTattatt                                                               <  4:34654/151‑1 (MQ=255)

                                                                                      ccgATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGc                                                             >  1:730388/1‑151 (MQ=255)

                                                                                       cgATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGcc                                                            >  4:1152858/1‑151 (MQ=255)

                                                                                        gATCTCAATGACAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTAGCCATACCCGGAGCGGCGCTATTTATTATTGCCt                                                           >  3:13283/1‑151 (MQ=255)

                                                                                         aTCTCAATGCCAGCGCAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGGTGAAAGCTGCGGTGTTATTTGCCGGCAAAAAGGCCGATCGTTCAGGGGGAAAACCGGCCAGCATACCCGTCGCGGCGCGATTTATTATTGCCTc                                                          >  3:399141/1‑151 (MQ=255)

                                                                                           ctcAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCgg                                                        >  2:540812/1‑151 (MQ=255)

                                                                                             cAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGc                                                             <  4:981151/144‑1 (MQ=255)

                                                                                             cAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTc                                                          <  4:620698/147‑1 (MQ=255)

                                                                                             cAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCgg                                                        <  1:845383/149‑1 (MQ=255)

                                                                                             cAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGgt                                                       <  2:477723/150‑1 (MQ=255)

                                                                                              aaTGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGgtgt                                                     <  4:1883819/151‑1 (MQ=255)

                                                                                              aaTGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGgtgt                                                     >  4:1411518/1‑151 (MQ=255)

                                                                                              aaTGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGGATATCTGTCGGGGATGACAGCTGCGGTGTTATTTGCCGGTAAAGTGGCCGATCGGTCCGGGAGAAAGCCGGTCGCCATACCCGGCGCAGCGCTATTTATTATTGCCTCGgcgt                                                     >  3:309835/1‑151 (MQ=255)

                                                                                               aTGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGtgtt                                                    <  1:425798/151‑1 (MQ=255)

                                                                                                 gCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGtgttct                                                  >  1:555998/1‑151 (MQ=255)

                                                                                                     gCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGtgttctgtt                                               <  1:674475/150‑1 (MQ=255)

                                                                                                        aaGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCAc                                            >  1:1022047/1‑150 (MQ=255)

                                                                                                            gcAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCt                                       >  1:850456/1‑151 (MQ=255)

                                                                                                             cAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTg                                      >  4:1306100/1‑151 (MQ=255)

                                                                                                             cAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTg                                      >  1:707020/1‑151 (MQ=255)

                                                                                                              aGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTg                                      <  4:1410507/150‑1 (MQ=255)

                                                                                                              aGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGa                                     <  1:232011/151‑1 (MQ=255)

                                                                                                                    atatTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAg                               <  3:372083/151‑1 (MQ=255)

                                                                                                                      atTGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCTATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCa                             <  1:1440854/151‑1 (MQ=255)

                                                                                                                        tGCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACg                           >  2:305350/1‑151 (MQ=255)

                                                                                                                         gCGTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGt                          >  1:936013/1‑151 (MQ=255)

                                                                                                                           gTTCTCCGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTTTTTATTATTGCCTCGGAGTTCTGTTCACTGGCTGAAACCAGCACGtt                         >  1:1346691/1‑150 (MQ=255)

                                                                                                                                ccGTATATCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTAtt                      <  3:19188/148‑1 (MQ=255)

                                                                                                                                  gTATAGCTGGCGGTGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAATCCGGTCGCCATACCCGGCGCTGCGCTATTTATTATTGCCTCGGTGTGCTGTCCACTGGCTGAAACCAGCACGTTATTTCtag                 <  3:99739/151‑3 (MQ=255)

                                                                                                                                   tataTCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGc                >  3:513731/1‑151 (MQ=255)

                                                                                                                                      atCTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAg              <  2:309725/150‑1 (MQ=255)

                                                                                                                                        cTGGCGGGGATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGcc           >  4:1664333/1‑151 (MQ=255)

                                                                                                                                             ggggATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGAttt      >  4:1699882/1‑151 (MQ=255)

                                                                                                                                               ggATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCt    <  2:422399/151‑1 (MQ=255)

                                                                                                                                                gATGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTa   <  4:601554/151‑1 (MQ=255)

                                                                                                                                                 aTGACAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTAc  >  3:521322/1‑151 (MQ=255)

CCGCTTTTTGATTTGTAGTTTTGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACCGCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGGCAGCTGCGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTAC  >  NC_000913/3891620‑3891915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`