Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,003,303 A→G R292R (CGT→CGC)  rarD ← putative chloramphenical resistance permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,003,3030AG99.4% 574.0 / NA 157R292R (CGT→CGC) rarDputative chloramphenical resistance permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (95/60);  minor base C (1/0);  total (97/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 157 total aligned reads displayed.

ATCTTACCGTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAA  >  NC_000913/4003156‑4003449
                                                                                                                                                   |                                                                                                                                                  
       ccttAGATGTTGGGATAAATATACTTTACATAAATTTTACCTCCCTGTATGAGTCCCCTGGCAGCATGGAAATATTCCCACTCTCATGCAAGAATCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGta                                                                                                                                           <  1:737003/148‑1 (MQ=255)
         ttAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGtata                                                                                                                                         >  1:962210/1‑150 (MQ=255)
          tAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATaa                                                                                                                                        >  1:738516/1‑150 (MQ=255)
           aGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAAt                                                                                                                                      >  1:439695/1‑151 (MQ=255)
            gATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATc                                                                                                                                     >  4:1148462/1‑151 (MQ=255)
            gATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATc                                                                                                                                     <  1:8799/151‑1 (MQ=255)
                 tGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGcatc                                                                                                                                >  4:700346/1‑151 (MQ=255)
                 tGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGcatc                                                                                                                                >  2:339656/1‑151 (MQ=255)
                  gggATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGATTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTTGGCTGCGTATAAATCGcatcc                                                                                                                               >  3:160063/1‑151 (MQ=255)
                  gggATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGcatcc                                                                                                                               <  1:1309032/151‑1 (MQ=255)
                  gggATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGcatc                                                                                                                                <  4:1444293/150‑1 (MQ=255)
                       aaaTATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATca                                                                                                                           <  1:640186/150‑1 (MQ=255)
                        aaTATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATcaca                                                                                                                         <  1:1157974/151‑1 (MQ=255)
                         atatACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATcaca                                                                                                                         >  4:720693/1‑150 (MQ=255)
                          tataCGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTCCTGCGTTGCGTATAAATCGCATCCATCACaa                                                                                                                        >  1:10569/1‑150 (MQ=255)
                           ataCGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACaaa                                                                                                                       >  1:408579/1‑150 (MQ=255)
                           ataCGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACaaa                                                                                                                       >  4:1140813/1‑150 (MQ=255)
                           ataCGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACaaa                                                                                                                       <  4:1297023/150‑1 (MQ=255)
                            taCGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACaaaa                                                                                                                      >  1:196064/1‑150 (MQ=255)
                             aCGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTGTGCGCTGCGTATAAATCGCATCCATCACaaaaa                                                                                                                     <  4:1475121/150‑1 (MQ=255)
                                      aaaTTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAgcgc                                                                                                           >  4:974414/1‑151 (MQ=255)
                                       aaTTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGcc                                                                                                          >  2:412503/1‑151 (MQ=255)
                                         ttttACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCAc                                                                                                        >  4:1240344/1‑151 (MQ=255)
                                            tACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCAccc                                                                                                      >  4:491954/1‑150 (MQ=255)
                                            tACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCAccc                                                                                                      >  1:1254450/1‑150 (MQ=255)
                                            tACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCAccc                                                                                                      >  1:908142/1‑150 (MQ=255)
                                            tACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATACATCACAAAAATTGCCAGCGCCAccc                                                                                                      >  4:1201324/1‑150 (MQ=255)
                                              cATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCaa                                                                                                    >  3:437651/1‑150 (MQ=255)
                                                  cTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATaa                                                                                                >  3:368306/1‑150 (MQ=255)
                                                    tGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAg                                                                                              <  1:1579021/150‑1 (MQ=255)
                                                       aTGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAgggg                                                                                           <  1:1020670/150‑3 (MQ=255)
                                                       aTGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGa                                                                                          <  4:439852/151‑1 (MQ=255)
                                                         gAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGaa                                                                                         <  1:1759084/150‑1 (MQ=255)
                                                          aGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGaa                                                                                         >  1:518532/1‑149 (MQ=255)
                                                                    cAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCtt                                                                             >  2:29143/1‑151 (MQ=255)
                                                                      gCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTAt                                                                           <  1:1582697/151‑1 (MQ=255)
                                                                       cATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATc                                                                          >  1:761822/1‑151 (MQ=255)
                                                                       cATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATc                                                                          >  4:1380198/1‑151 (MQ=255)
                                                                       cATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATc                                                                          >  4:1275364/1‑151 (MQ=255)
                                                                           gCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGGCGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCa                                                                      <  3:44799/151‑1 (MQ=255)
                                                                             aaTATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCAcc                                                                    >  1:1471223/1‑151 (MQ=255)
                                                                              atatGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCAccc                                                                   <  4:148441/151‑1 (MQ=255)
                                                                                atGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCgg                                                                 <  1:1202038/151‑1 (MQ=255)
                                                                                  gCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGtt                                                               <  1:1534716/151‑1 (MQ=255)
                                                                                   cccACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGttt                                                              <  1:1492268/151‑1 (MQ=255)
                                                                                   cccACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGttt                                                              <  2:429145/151‑1 (MQ=255)
                                                                                    ccACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGtttt                                                             <  1:1467784/151‑1 (MQ=255)
                                                                                     cACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGttttt                                                            >  4:518971/1‑151 (MQ=255)
                                                                                       ctctCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTc                                                           <  4:1609552/150‑1 (MQ=255)
                                                                                        tctcATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCAc                                                         >  1:833833/1‑151 (MQ=255)
                                                                                              gCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATaa                                                    >  2:248430/1‑150 (MQ=255)
                                                                                              gCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATaa                                                    >  4:1694837/1‑150 (MQ=255)
                                                                                               cAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATaaaa                                                  <  4:1502871/151‑1 (MQ=255)
                                                                                                aaGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAAc                                                 >  3:182564/1‑151 (MQ=255)
                                                                                                 agagTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTCCGCGGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAATTAAAGGCGAAAGTCACCATCTTATCGGCCCCCGGTTTTTCACCATAAAAc                                                 >  3:517934/1‑150 (MQ=255)
                                                                                                    gTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTca                                             <  4:402108/151‑1 (MQ=255)
                                                                                                      cGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTcac                                            >  4:1502299/1‑150 (MQ=255)
                                                                                                       ggCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTcac                                            >  4:725908/1‑149 (MQ=255)
                                                                                                        gCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCAcagc                                         >  1:1719113/1‑151 (MQ=255)
                                                                                                            atTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCAcagccag                                      <  1:928471/150‑1 (MQ=255)
                                                                                                              tttttCAGAATATATTTATTTTTTATTTGGCCGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCcagca                                    <  4:242885/150‑1 (MQ=255)
                                                                                                              tttttCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCAcagccagc                                     >  3:38134/1‑149 (MQ=255)
                                                                                                              tttttCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCAcagccagc                                     >  4:1551672/1‑149 (MQ=255)
                                                                                                              tttttCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCAcagccagc                                     >  4:1844535/1‑149 (MQ=255)
                                                                                                              tttttCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCAcagccagc                                     >  2:196554/1‑149 (MQ=255)
                                                                                                              tttttCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCcagca                                    <  4:1770108/150‑1 (MQ=255)
                                                                                                                tttCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAgg                                  <  4:738838/150‑1 (MQ=255)
                                                                                                                 ttCAGACTATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGa                                 >  2:163948/1‑150 (MQ=255)
                                                                                                                 ttCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGa                                 >  1:987534/1‑150 (MQ=255)
                                                                                                                  tCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAAc                               >  1:788528/1‑151 (MQ=255)
                                                                                                                  tCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAAc                               >  1:229504/1‑151 (MQ=255)
                                                                                                                   cAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAAc                               <  4:1653745/150‑1 (MQ=255)
                                                                                                                   cAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAAc                               >  4:1661104/1‑150 (MQ=255)
                                                                                                                      aaTATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATc                            >  4:1151519/1‑150 (MQ=255)
                                                                                                                      aaTATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATc                            >  1:658161/1‑150 (MQ=255)
                                                                                                                        tatatTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGc                         >  4:561786/1‑151 (MQ=255)
                                                                                                                           atttatttTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGt                       <  1:1316015/150‑1 (MQ=255)
                                                                                                                           atttatttTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTc                      <  3:412489/151‑1 (MQ=255)
                                                                                                                             ttattttttatttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCgg                    >  1:54879/1‑151 (MQ=255)
                                                                                                                               attttttatttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCGCCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCg                     <  1:1878544/148‑1 (MQ=255)
                                                                                                                               attttttatttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCggg                   <  1:321512/150‑1 (MQ=255)
                                                                                                                               attttttatttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGc                  >  4:1080796/1‑151 (MQ=255)
                                                                                                                                  ttttatttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCa                >  4:1612227/1‑150 (MQ=255)
                                                                                                                                    ttatttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATg             >  3:61408/1‑151 (MQ=255)
                                                                                                                                    ttatttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATg             >  3:15391/1‑151 (MQ=255)
                                                                                                                                     tatttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGt            >  1:900571/1‑151 (MQ=255)
                                                                                                                                      atttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGt            <  1:700044/150‑1 (MQ=255)
                                                                                                                                      atttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGt            >  1:932531/1‑150 (MQ=255)
                                                                                                                                      atttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTa           <  1:747156/151‑1 (MQ=255)
                                                                                                                                       tttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTAc          >  4:37549/1‑151 (MQ=255)
                                                                                                                                       tttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTAc          >  3:330003/1‑151 (MQ=255)
                                                                                                                                         tGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTg        >  1:1062929/1‑151 (MQ=255)
                                                                                                                                          ggACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTg        <  4:248819/150‑1 (MQ=255)
                                                                                                                                            aCGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGa      >  3:194754/1‑150 (MQ=255)
                                                                                                                                            aCGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGa      >  2:521308/1‑150 (MQ=255)
                                                                                                                                             cGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCTCCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGAGCCAATGTACTGGaa     <  1:1439091/150‑1 (MQ=255)
                                                                                                                                             cGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGaa     >  1:550505/1‑150 (MQ=255)
                                                                                                                                             cGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGaa     >  3:337625/1‑150 (MQ=255)
                                                                                                                                               ttCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGaaaa   >  1:3895/1‑150 (MQ=255)
                                                                                                                                                tCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGaaaaa  <  4:578491/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATCTTACCGTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAA  >  NC_000913/4003156‑4003449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑