Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 295,230 G→A T117M (ACG→ATG)  yagN ← CP4‑6 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913295,2300GA100.0% 559.7 / NA 142T117M (ACG→ATG) yagNCP4‑6 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (72/70);  total (72/70)

Only 100 of 142 total aligned reads displayed.

AACTCAGCTGTTGAAATGAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACGTTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAGTTAGTAGCATTTTGCATTC  >  NC_000913/295084‑295377
                                                                                                                                                  |                                                                                                                                                   
aaCTCAGCTGTTGAAATGAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACAttt                                                                                                                                                  <  4:1550233/150‑1 (MQ=255)
aaCTCAGCTGTTGAAATGAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACAtt                                                                                                                                                   <  2:323923/149‑1 (MQ=255)
 aCTCAGCTGTTGAAATGAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCg                                                                                                                                                >  2:493461/1‑151 (MQ=255)
 aCTCAGCTGTTGAAATGAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCg                                                                                                                                                <  4:1562756/151‑1 (MQ=255)
  cTCAGCTGTTGAAATGAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCgg                                                                                                                                               >  4:630368/1‑151 (MQ=255)
   tCAGCTGTTGAAATGAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCggg                                                                                                                                              >  4:221853/1‑151 (MQ=255)
    cAGCTGTTGAAATGAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCgg                                                                                                                                               <  1:1089046/149‑1 (MQ=255)
         gTTGAAATGAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCtcgg                                                                                                                                         <  4:939173/150‑1 (MQ=255)
          ttGAAATGAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCtcgggg                                                                                                                                       >  1:913238/1‑151 (MQ=255)
            gAAATGAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCtcggggtc                                                                                                                                     <  4:1738877/151‑1 (MQ=255)
            gAAATGAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCtcggggtc                                                                                                                                     <  4:1472873/151‑1 (MQ=255)
            gAAATGAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCtcggggtc                                                                                                                                     <  4:765522/151‑1 (MQ=255)
               aTGAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCtcggggtcggg                                                                                                                                  >  2:190035/1‑151 (MQ=255)
                 gAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTcggggtcggggt                                                                                                                                >  1:228656/1‑151 (MQ=255)
                  aGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCggggtcggggtc                                                                                                                               >  1:1368000/1‑151 (MQ=255)
                  aGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCggggtcggggtc                                                                                                                               <  4:1260260/151‑1 (MQ=255)
                      gtgATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTa                                                                                                                           <  1:1682682/151‑1 (MQ=255)
                         aTTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTAcc                                                                                                                         >  4:1005460/1‑150 (MQ=255)
                             agaTGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCAc                                                                                                                    <  2:391180/151‑1 (MQ=255)
                               aTGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCAc                                                                                                                    >  3:287540/1‑149 (MQ=255)
                               aTGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCt                                                                                                                  >  2:12411/1‑151 (MQ=255)
                                   aTCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGaa                                                                                                               <  4:440369/150‑1 (MQ=255)
                                    tCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATc                                                                                                             >  1:441638/1‑151 (MQ=255)
                                     ccGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCt                                                                                                            >  3:391440/1‑151 (MQ=255)
                                      tgggAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCtg                                                                                                           >  3:412112/2‑151 (MQ=255)
                                       gggAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCtg                                                                                                           >  1:1925972/1‑150 (MQ=255)
                                          aGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCtgatga                                                                                                       <  1:1758802/151‑1 (MQ=255)
                                           gTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTgatgat                                                                                                      <  1:198590/151‑1 (MQ=255)
                                                  tGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCAc                                                                                               >  4:854300/1‑151 (MQ=255)
                                                      cTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACtttt                                                                                           >  2:42826/1‑151 (MQ=255)
                                                       tCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACtttt                                                                                           >  2:208595/1‑150 (MQ=255)
                                                         aTTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAg                                                                                         >  3:302963/1‑150 (MQ=255)
                                                         aTTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGc                                                                                        >  4:444196/1‑151 (MQ=255)
                                                         aTTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGc                                                                                        <  4:1242844/151‑1 (MQ=255)
                                                          ttttGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCt                                                                                       >  4:192035/1‑151 (MQ=255)
                                                           tttGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCt                                                                                       <  4:1049293/150‑1 (MQ=255)
                                                              accACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCtcggg                                                                                                                                        <  2:35245/97‑1 (MQ=255)
                                                               ccACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGAttt                                                                                  >  1:222145/1‑151 (MQ=255)
                                                                        tGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACt                                                                         >  1:1639221/1‑151 (MQ=255)
                                                                         gATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACtg                                                                        >  1:418931/1‑151 (MQ=255)
                                                                          aTTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACt                                                                         >  3:2598/1‑149 (MQ=255)
                                                                            tGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACtgtg                                                                      >  3:742/1‑150 (MQ=255)
                                                                             gagaTATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGAt                                                                    >  1:1615373/1‑151 (MQ=255)
                                                                               gaTATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGc                                                                  <  1:399108/151‑1 (MQ=255)
                                                                                atatTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATg                                                                   <  2:26151/149‑1 (MQ=255)
                                                                                atatTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCg                                                                 >  1:970468/1‑151 (MQ=255)
                                                                                     ggCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCt                                                             <  1:1298085/150‑1 (MQ=255)
                                                                                     ggCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTg                                                            <  1:800316/151‑1 (MQ=255)
                                                                                     ggCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTg                                                            <  1:1342626/151‑1 (MQ=255)
                                                                                      gCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTgg                                                           >  1:1502984/1‑151 (MQ=255)
                                                                                      gCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTgg                                                           <  1:1428079/151‑1 (MQ=255)
                                                                                      gCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTgg                                                           <  3:151108/151‑1 (MQ=255)
                                                                                       cAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGa                                                          <  1:610848/151‑1 (MQ=255)
                                                                                        aaagaaagCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGAt                                                         <  3:483041/151‑1 (MQ=255)
                                                                                          agaaagCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGc                                                       <  1:1185535/151‑1 (MQ=255)
                                                                                           gaaagCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGcc                                                      <  1:106551/151‑1 (MQ=255)
                                                                                              agCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGa                                                   <  1:644731/151‑1 (MQ=255)
                                                                                               gCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGACGCCTGAt                                                  <  1:685737/151‑1 (MQ=255)
                                                                                                cTTTAGGCATTATTGAATTAGGAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGAtt                                                 >  1:1827547/1‑151 (MQ=255)
                                                                                                cTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGAtt                                                 >  4:118202/1‑151 (MQ=255)
                                                                                                cTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGAtt                                                 >  4:1813475/1‑151 (MQ=255)
                                                                                                 tttAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGAttt                                                >  3:337800/1‑151 (MQ=255)
                                                                                                  ttAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGAtt                                                 <  2:407700/149‑1 (MQ=255)
                                                                                                    aaGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTa                                             <  2:413897/151‑1 (MQ=255)
                                                                                                    aaGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTa                                             <  1:946732/151‑1 (MQ=255)
                                                                                                      gCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTAc                                            >  2:128609/1‑150 (MQ=255)
                                                                                                      gCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTAc                                            >  4:1234565/1‑150 (MQ=255)
                                                                                                        attattGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGATGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACAt                                          <  4:1506805/150‑1 (MQ=255)
                                                                                                           attGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGAc                                       <  4:947871/150‑1 (MQ=255)
                                                                                                             tGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACAt                                     <  2:103653/150‑1 (MQ=255)
                                                                                                             tGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATc                                    >  1:1726461/1‑151 (MQ=255)
                                                                                                              gAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATc                                    <  4:1626504/150‑1 (MQ=255)
                                                                                                              gAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATc                                    >  2:303168/1‑150 (MQ=255)
                                                                                                               acttAGTAGGGGTAGTAATGAAGTCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGCTCTGGATGCCTGATTTTTACATGACAt                                     >  1:284706/3‑148 (MQ=255)
                                                                                                               aaTTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAg                                  <  1:1408544/151‑1 (MQ=255)
                                                                                                                 ttAGTAGGGGTCGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCCGGCTGCATGATTTTTACATGCCATCAGa                                 >  3:351934/1‑150 (MQ=255)
                                                                                                                 ttAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGAc                                >  2:31884/1‑151 (MQ=255)
                                                                                                                       ggggTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCga                          <  3:546931/151‑1 (MQ=255)
                                                                                                                        gggTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGAcgcgcg                           >  4:922611/1‑149 (MQ=255)
                                                                                                                          gtagtaATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGaa                       <  1:453819/151‑1 (MQ=255)
                                                                                                                            agtaATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGaaa                      <  2:264969/150‑1 (MQ=255)
                                                                                                                            agtaATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAg                     <  1:700299/151‑1 (MQ=255)
                                                                                                                               aaTGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAGtt                   <  3:493442/150‑1 (MQ=255)
                                                                                                                                aTGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAGTtag                 <  1:1045819/151‑1 (MQ=255)
                                                                                                                                  gAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACGTCAGACGCGCGAGAAAGTtagt                >  3:436952/1‑150 (MQ=255)
                                                                                                                                   aaGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAGTtagtag              <  1:29106/151‑1 (MQ=255)
                                                                                                                                   aaGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAGTtagt                >  4:1476017/1‑149 (MQ=255)
                                                                                                                                       cAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAGTTAGTAGCAtt          >  4:1841397/1‑151 (MQ=255)
                                                                                                                                       cAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAGTTAGTAGCAtt          <  4:419081/151‑1 (MQ=255)
                                                                                                                                       cAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAGTTAGTAGCAtt          >  4:507650/1‑151 (MQ=255)
                                                                                                                                       cAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAGTTAGTAGCAtt          >  1:1187924/1‑151 (MQ=255)
                                                                                                                                        aGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAg                     >  1:517452/1‑139 (MQ=255)
                                                                                                                                        aGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAg                     <  4:517658/139‑1 (MQ=255)
                                                                                                                                        aGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAGTTAGTAGCAttt         <  4:1852535/151‑1 (MQ=255)
                                                                                                                                        aGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAGTTAGTAGCAttt         <  2:23607/151‑1 (MQ=255)
                                                                                                                                        aGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAGTTAGTAGCAttt         <  4:694101/151‑1 (MQ=255)
                                                                                                                                        aGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAGTTAGTAGCAttt         >  4:659120/1‑151 (MQ=255)
                                                                                                                                           aGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAGTTAGTAGCATTTTGc      >  1:1895631/1‑151 (MQ=255)
                                                                                                                                              aaaCATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAGTTAGTAGCATTTTGCAtt   >  4:588912/1‑151 (MQ=255)
                                                                                                                                               aaCATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAGTTAGTAGCATTTTGCATTc  <  4:1267029/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AACTCAGCTGTTGAAATGAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACGTTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAGTTAGTAGCATTTTGCATTC  >  NC_000913/295084‑295377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑