BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,343,909	A→G	M181V (ATG→GTG)	melB →	melibiose:sodium symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,343,909	0	A	G	100.0%	547.5 / NA	155	M181V (ATG→GTG)	melB	melibiose:sodium symporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (72/83); total (72/83)

Only 100 of 155 total aligned reads displayed.

CAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGATGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAATTTATAAAA  >  NC_000913/4343763‑4344056

cAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGtt                                                                                                                                                 <  4:1250582/151‑1 (MQ=255)

       cACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCActct                                                                                                                                           <  4:668975/150‑1 (MQ=255)

       cACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTg                                                                                                                                          <  1:1275616/151‑1 (MQ=255)

        aCGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCActct                                                                                                                                           <  4:1174559/149‑1 (MQ=255)

        aCGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTgg                                                                                                                                         <  1:799317/151‑1 (MQ=255)

             cGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTAc                                                                                                                                      <  4:397293/149‑1 (MQ=255)

             cGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTg                                                                                                                                    <  1:333164/151‑1 (MQ=255)

             cGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGGGGGGGT‑TGGGGAATTTGGTTTTC‑GGGGTTCACTCTGGTACt                                                                                                                                     >  3:136609/1‑150 (MQ=255)

                  aaCGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGTG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATc                                                                                                                                 <  1:1076945/149‑1 (MQ=255)

                   aCGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGGGGGGGT‑CGGGG‑GTTTTGGTTTTTGGTGTTTCCTCTGGTACTGGTTGcc                                                                                                                              >  1:1109138/1‑151 (MQ=255)

                    cGTGAGCGCGAACCACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTCTCAGGTGTTCACTCTGGTACTGATCGCCt                                                                                                                             <  4:303903/151‑1 (MQ=255)

                    cGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGGGGT‑CGGGG‑GTTTGGCTTTTAAGGGTTCACTCTTGGACTGGTTGcc                                                                                                                              >  4:228242/1‑150 (MQ=255)

                     gTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCtt                                                                                                                            <  1:1389994/151‑1 (MQ=255)

                       gAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑GTTTTGCTTTCAGGTGTTTACTCTGGTACTGGTCGCCtttt                                                                                                                          >  4:447558/1‑151 (MQ=255)

                        aGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCttttt                                                                                                                         <  1:1218969/151‑1 (MQ=255)

                             gAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGGT‑CGGGG‑GTTTGGCTTTTAGGTGTTCACCCTTGGAATGATCGCCTtttttttt                                                                                                                     >  1:79081/1‑150 (MQ=255)

                             gAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTg                                                                                                                    <  3:404549/151‑1 (MQ=255)

                                caacTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGttt                                                                                                                 <  4:145916/151‑1 (MQ=255)

                                 aacTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑GTTTGGCTTTCAGGGGTTCACTCTGGTACTGATCGCCTTTTTTAtttttt                                                                                                                 >  2:35283/1‑150 (MQ=255)

                                 aacTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTc                                                                                                                <  1:1337624/151‑1 (MQ=255)

                                 aacTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTc                                                                                                                <  1:1173820/151‑1 (MQ=255)

                                 aacTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGGGGCGGA‑CGGGG‑GTTTTGGCTTCAGGGGGTTTCTCTTGTACTGTTCGCCTtttttttttttt                                                                                                                 >  1:850084/1‑150 (MQ=255)

                                 aacTGGTTACTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTAACATTTGTTAATTATGTCGGC‑GGGGGGGATAGGGGG‑ATTTGG‑TTTTAGGGGTTTGCTTTGGGACTGGTCGGCTTTTTTATTGtttt                                                                                                                >  4:541796/1‑150 (MQ=255)

                                     ggTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑GTTTTGCTTTTAGGGGTTCACTCTGGGACTGATCGCCTTTTTTTTTGTTTTaaac                                                                                                            >  4:696273/1‑149 (MQ=255)

                                     ggTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCaa                                                                                                              <  1:263404/149‑1 (MQ=255)

                                      gTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACca                                                                                                           <  4:1454425/151‑1 (MQ=255)

                                       ttCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGGGGCGGT‑TGGGG‑AGTTGGGCTTTATGTGGTTACTCTTGGACTGTTTGCCTttttttttttttttacc                                                                                                            >  1:1272971/1‑144 (MQ=255)

                                         ccTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACcatca                                                                                                        <  4:1410591/151‑1 (MQ=255)

                                         ccTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACcatca                                                                                                        <  1:1825180/151‑1 (MQ=255)

                                         ccTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGGGGGGGG‑CGGGG‑GTTTGGCTTTTCGGGGTTTTCTCTGGGAAAGATTGCCTTTTTTAttttttttaac                                                                                                            >  1:265339/1‑142 (MQ=255)

                                          cTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGGGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑GTTTTGCTTTTCGGGGTTTACTCTGGGACTGATCGCCTTTTTTTTTGTTTTAACcctc                                                                                                         >  2:253297/1‑149 (MQ=255)

                                            tATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACcatcat                                                                                                       <  1:949762/149‑1 (MQ=255)

                                            tATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGGGTGACGCTACCATTTGTTAATTATGTCGGC‑GGGGGGGGT‑CGGGG‑GTTTTGCTTTCAGGGGTTCACTCTGGTACTGATCGCCTTTTTTTTTGTTTCAAAca                                                                                                           >  4:69173/1‑145 (MQ=255)

                                             aTCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGGGGGGAT‑CGGGG‑GTTTTGGTTTTAGGGGTTTACTCTTGTACTGGTCGCCTTTTTTTTTTTTTTAAc                                                                                                             >  3:346296/1‑142 (MQ=255)

                                               ccGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCAct                                                                                                   <  4:1709312/150‑1 (MQ=255)

                                                 gcgTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTg                                                                                                <  1:1452685/151‑1 (MQ=255)

                                                 gcgTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTg                                                                                                <  4:25774/151‑1 (MQ=255)

                                                  cgTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTgc                                                                                               <  4:1928775/151‑1 (MQ=255)

                                                   gTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTgcg                                                                                              <  2:136615/151‑1 (MQ=255)

                                                     ttttttGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGGGAT‑CGGGG‑GGTTTGGTTTTAGGGGTTCACTCTGGGACTGTTTGCCTTTTTTTTTGTTTCAAACATTCTCActcttc                                                                                               >  1:17210/1‑146 (MQ=255)

                                                       ttttGCCAGTCTGGCAGGCTTTGTTACGGCAGGGGTGACGCTACCATTTGTTAATTATGGCGGC‑GGAGGCGAT‑CGGGG‑GTTTGGGTTTTTGGGGGTTTCTCTGGTACTGGTCGCCTTTTTTTTTGTTTTAACCAGCATTTCTCTGTGCaa                                                                                           >  4:1559189/1‑150 (MQ=255)

                                                          tGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAAtgt                                                                                        <  4:930185/150‑1 (MQ=255)

                                                          tGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAAtgt                                                                                        <  1:1566837/150‑1 (MQ=255)

                                                           gCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGGGGGGGTT‑GGGGG‑ATTTGCCTTTCAGGGGTTC‑CTCTTGTACTTATCGCCTTTTTTTTTTGTTTTACCAATATCActcctcggca                                                                                           >  3:491769/1‑139 (MQ=255)

                                                           gCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAAtgtg                                                                                       <  4:1560685/150‑1 (MQ=255)

                                                            ccAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCa                                                                                     <  4:214988/151‑1 (MQ=255)

                                                             cAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCCTTTGTTAAATATGTCGGC‑GGGGGCGGT‑CCGGG‑CTTTTGGTTTTAGGGGTTCACTCTGGGGCGGTTGGCCTTTTTTATTGTTTCAACCATCTTAACTATgcggtaagt                                                                                        >  4:442293/1‑141 (MQ=255)

                                                             cAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCAt                                                                                    <  3:143581/151‑1 (MQ=255)

                                                                tCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGa                                                                                  <  2:356145/150‑1 (MQ=255)

                                                                tCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGGGGGGAT‑CGGGG‑ATTTGGCTTTTAGGGGTTCACTCTGGTACTGATCGCCTTTTTTTTTGTTTCAACCCTCATCACCCTGCGCAAAGTGCATGa                                                                                  >  2:5363/1‑150 (MQ=255)

                                                                  tGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGGGGCGGT‑CGGGG‑GTTTGGCTTTTAGGTGTTTACTCTTGTACTGATCGCCTTTTTTTTTGTTTTAAACATCATCACTCTTCGCAATGTGCATGaa                                                                                 >  4:1338260/1‑149 (MQ=255)

                                                                    gCAGGCTTTGTTACGGCAGGGGTGACGCTACCATTTGTTAATTATGTCGGC‑GGGGGCGAT‑TGGGG‑GGTTGGGTTTCAGGGGGTCAATCTGGTAATGGTTGCCTTTTTTTTTGTTTTAACCCTCATCACTCTTCGCAAAGTGgaagaa                                                                                 >  4:1231239/1‑147 (MQ=255)

                                                                      aGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCttt                                                                           <  4:1927357/151‑1 (MQ=255)

                                                                      aGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCttt                                                                           <  4:600570/151‑1 (MQ=255)

                                                                       ggCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑GTTTGGCTTTCAGGGGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCtttt                                                                          >  2:393316/1‑151 (MQ=255)

                                                                          tttGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGGT‑CGGGG‑GTTTGGCTTTTAGGGGTTTACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCtttt                                                                          >  2:140868/1‑148 (MQ=255)

                                                                          tttGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGGGGCGAT‑CGGGG‑GTTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTTTTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCtttt                                                                          >  4:1589126/1‑148 (MQ=255)

                                                                           ttGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑GTTTTGCTTTTAGGTGTTTACTCTGGGACTGATCGCCTTTTTTATTGTTTTAACCATCATCACTCTGCGCAATGTGCATGAAGTCtttt                                                                          >  4:92418/1‑147 (MQ=255)

                                                                                aCGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGAc                                                                  <  1:331076/150‑1 (MQ=255)

                                                                                 tggCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTTGTCAGACa                                                                 >  3:504161/2‑150 (MQ=255)

                                                                                 cGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACa                                                                 >  4:639082/1‑150 (MQ=255)

                                                                                      ggTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTTCCTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCaa                                                            >  3:521520/1‑150 (MQ=255)

                                                                                      ggTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAAc                                                           <  4:1693047/151‑1 (MQ=255)

                                                                                        tgtgACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑TGGGG‑ATTTTGCTTTCAGGTGTTTACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCg                                                         >  3:465188/1‑151 (MQ=255)

                                                                                         gtgACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGt                                                        <  4:844693/151‑1 (MQ=255)

                                                                                            aCGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTct                                                      <  1:1233052/150‑1 (MQ=255)

                                                                                            aCGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTct                                                      >  1:1594926/1‑150 (MQ=255)

                                                                                              gCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTctgct                                                   <  4:5987/151‑1 (MQ=255)

                                                                                                 aCCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTga                                                 >  1:1418980/1‑150 (MQ=255)

                                                                                                  ccATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTga                                                 >  1:480235/1‑149 (MQ=255)

                                                                                                   cATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTgaagg                                              >  1:1716886/1‑151 (MQ=255)

                                                                                                   cATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCCTTCAGGGGTTCACTCTGGTACTGGTCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTTGTCAGACAATCAACCGTCTGCTgaagg                                              >  1:947786/1‑151 (MQ=255)

                                                                                                    aTTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTTCCTCTGGTACTTATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCATACAATCAACCGTCTGCTgaagg                                              >  4:1200420/1‑150 (MQ=255)

                                                                                                    aTTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTgaagg                                              >  4:1852970/1‑150 (MQ=255)

                                                                                                       tGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTgaaggaag                                           <  4:191136/150‑1 (MQ=255)

                                                                                                          tAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCAt                                       >  2:292866/1‑151 (MQ=255)

                                                                                                            aTTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCt                                     <  4:899089/151‑1 (MQ=255)

                                                                                                            aTTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTAATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCt                                     >  3:168759/1‑151 (MQ=255)

                                                                                                                 gTCGGC‑GGTGGCGGT‑CGGGG‑ATTTTGCTTTTAGGGGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAACCATCTGaca                                 >  1:108674/1‑150 (MQ=255)

                                                                                                                 gTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGaca                                 <  2:491788/150‑1 (MQ=255)

                                                                                                                 gTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGaca                                 <  4:532382/150‑1 (MQ=255)

                                                                                                                      c‑ggTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTaa                            >  4:779872/1‑150 (MQ=255)

                                                                                                                        ggTGGCGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTaa                            >  4:1053294/1‑149 (MQ=255)

                                                                                                                             cGAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAACCCATc                     >  4:615700/1‑151 (MQ=255)

                                                                                                                              gAT‑CGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCg                    <  4:1345124/151‑1 (MQ=255)

                                                                                                                                  cGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGtt                  <  1:1771970/150‑1 (MQ=255)

                                                                                                                                  cGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTg                 <  3:81319/151‑1 (MQ=255)

                                                                                                                                  cGGGG‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTg                 <  1:1561086/151‑1 (MQ=255)

                                                                                                                                    ggg‑ATTTGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTgcg               <  4:361523/151‑1 (MQ=255)

                                                                                                                                          ttGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAAt          >  3:255892/1‑151 (MQ=255)

                                                                                                                                           tGGCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAAtt         >  3:254944/1‑151 (MQ=255)

                                                                                                                                            ggCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAAttt        >  4:1781233/1‑151 (MQ=255)

                                                                                                                                            ggCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAAttt        <  1:1352621/151‑1 (MQ=255)

                                                                                                                                             gCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAAtt         <  3:114004/149‑1 (MQ=255)

                                                                                                                                             gCTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAATTta       <  4:1789198/151‑1 (MQ=255)

                                                                                                                                              cTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAATTtat      >  1:1595899/1‑151 (MQ=255)

                                                                                                                                              cTTTCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAATTtat      >  1:470462/1‑151 (MQ=255)

                                                                                                                                                ttCAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAATTtata     <  4:31758/150‑1 (MQ=255)

                                                                                                                                                  cAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAATTTATaaaa  <  4:1015767/151‑1 (MQ=255)

                                                                                                                                                  cAGGTGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAATTTATaaa   <  1:187586/150‑1 (MQ=255)

CAACCATCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGC‑GGTGGCGAT‑CGGGG‑ATTTGGCTTTCAGATGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAATTTATAAAA  >  NC_000913/4343763‑4344056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`