BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,350,244	A→G	V473V (GTT→GTC)	dcuS ←	sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,350,244	0	A	G	100.0%	586.6 / NA	150	V473V (GTT→GTC)	dcuS	sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (85/65); total (85/65)

Only 100 of 150 total aligned reads displayed.

CCGATTCCACGGCGATGCTGCCGCCGAGATTTTCTACCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTAACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTCGCGACCTGGTCCTCACTGCCGCTGTC  >  NC_000913/4350096‑4350390

ccGATTCCACGGCGATGCTGCCGCCGAGATTTTCTACCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGAc                                                                                                                                                  >  3:284374/1‑151 (MQ=255)

   aTTCCACGGCGATGCTGCCGCCGAGATTTTCTACCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTc                                                                                                                                               <  1:402578/151‑1 (MQ=255)

     tCCACGGCGATGCTGCCGCCGAGATTTTCTACCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTca                                                                                                                                              <  1:8855/150‑1 (MQ=255)

       cACGGCGATGCTGCCGCCGAGATTTTCTACCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTcac                                                                                                                                             >  2:187351/1‑149 (MQ=255)

          ggCGATGCTGCCGCCGAGATTTTCTTCCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCAGGTGCGATCCCCCGTCCATCATCATTGGCTTCACAGTGc                                                                                                                                        <  1:280970/151‑1 (MQ=255)

           gCGATGCTGCCGCCGAGATTTTCTACCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGca                                                                                                                                       <  4:823288/151‑1 (MQ=255)

           gCGATGCTGCCGCCGAGATTTTCTACCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGc                                                                                                                                        >  4:805611/1‑150 (MQ=255)

             gATGCTGCCGCCGAGATTTTCTACCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGcagc                                                                                                                                     >  1:1788058/1‑151 (MQ=255)

                gctgcCGCCGAGATTTTCTACCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGcagcct                                                                                                                                   <  2:52025/150‑2 (MQ=255)

                gctgcCGCCGAGATTTTCTACCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGcagcc                                                                                                                                    >  4:765135/1‑149 (MQ=255)

                   gccgccGAGATTTTCTACCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCagccagcc                                                                                                                                <  3:266922/150‑1 (MQ=255)

                   gccgccGAGATTTTCTACCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCagccagcc                                                                                                                                <  1:193518/150‑1 (MQ=255)

                     cgccgAGATTTTCTACCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTg                                                                                                                             <  4:85975/151‑1 (MQ=255)

                      gccgAGATTTTCTACCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTg                                                                                                                             >  1:1064768/1‑150 (MQ=255)

                        cgAGATTTTCTACCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACg                                                                                                                          <  1:1022944/151‑1 (MQ=255)

                         gagaTTTTCTACCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACgg                                                                                                                         <  3:153248/151‑1 (MQ=255)

                            aTTTTCTACCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGt                                                                                                                        >  1:716796/1‑149 (MQ=255)

                            aTTTTCTACCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGt                                                                                                                        >  4:680089/1‑149 (MQ=255)

                              tttCTACCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTg                                                                                                                    >  1:550593/1‑151 (MQ=255)

                               ttCTACCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTg                                                                                                                    <  4:1386263/150‑1 (MQ=255)

                                   aCCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATg                                                                                                               <  4:1754315/151‑1 (MQ=255)

                                       gttgttTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTAc                                                                                                           <  4:212201/151‑1 (MQ=255)

                                          gttTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCt                                                                                                        >  4:427787/1‑151 (MQ=255)

                                          gttTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCt                                                                                                        <  4:277323/151‑1 (MQ=255)

                                              gACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAAtt                                                                                                    <  1:875414/151‑1 (MQ=255)

                                                        aaaCCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCCTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCgg                                                                                          <  4:851285/151‑1 (MQ=255)

                                                         aaCCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCggg                                                                                         >  1:1631250/1‑151 (MQ=255)

                                                         aaCCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCggg                                                                                         >  1:96202/1‑151 (MQ=255)

                                                           ccGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGtt                                                                                       <  2:205051/151‑1 (MQ=255)

                                                             gACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTcc                                                                                     >  4:1810152/1‑151 (MQ=255)

                                                              aCGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTcct                                                                                    <  3:203956/151‑2 (MQ=255)

                                                               cGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCgg                                                                                   <  1:1512112/151‑1 (MQ=255)

                                                                 ccTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGcc                                                                                 >  1:1390554/1‑151 (MQ=255)

                                                                   tcgctcgcTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCt                                                                               >  1:763328/1‑151 (MQ=255)

                                                                    cgctcgctTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGccc                                                                                <  1:847604/149‑1 (MQ=255)

                                                                    cgctcgctTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCt                                                                               >  1:968770/1‑150 (MQ=255)

                                                                     gctcgctTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTaa                                                                             <  1:1259142/151‑1 (MQ=255)

                                                                        cgctTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGc                                                                          >  1:228484/1‑151 (MQ=255)

                                                                           ttCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTc                                                                       >  3:263570/1‑151 (MQ=255)

                                                                           ttCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTc                                                                       >  4:712355/1‑151 (MQ=255)

                                                                           ttCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTc                                                                       >  3:293020/1‑151 (MQ=255)

                                                                               ttttGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAgc                                                                   >  1:480574/1‑151 (MQ=255)

                                                                               ttttGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCAGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAgc                                                                   >  4:513496/1‑151 (MQ=255)

                                                                                tttGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAgc                                                                   <  4:906325/150‑1 (MQ=255)

                                                                                 ttGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAgcg                                                                  <  1:1304478/150‑1 (MQ=255)

                                                                                  tGTCGAGACACCTTTGTCAAAAATGTGATCGCTTTTATCGGGTGCGAGCCCCGGTCCATCATCATTGACTTCCCAGTGCATCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCACCTCCGGGTTCCGTCCCTAATTCATCCAgcgc                                                                 <  3:74888/150‑1 (MQ=255)

                                                                                   gTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGt                                                               <  1:1476376/151‑1 (MQ=255)

                                                                                    tCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGtt                                                              >  1:1225729/1‑151 (MQ=255)

                                                                                    tCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGtt                                                              >  4:1730198/1‑151 (MQ=255)

                                                                                     cGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGtt                                                              <  1:17167/150‑1 (MQ=255)

                                                                                       agaCACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTc                                                           >  4:1243467/1‑151 (MQ=255)

                                                                                       agaCACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTc                                                           >  1:1690072/1‑151 (MQ=255)

                                                                                       agaCACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTc                                                           <  4:154153/151‑1 (MQ=255)

                                                                                        gaCACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCt                                                          >  2:440345/1‑151 (MQ=255)

                                                                                         acacCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTa                                                         <  1:554724/151‑1 (MQ=255)

                                                                                          cacCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTAt                                                        >  1:529252/1‑151 (MQ=255)

                                                                                           acCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATc                                                       >  1:1848823/1‑151 (MQ=255)

                                                                                               ttGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCATCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATc                                                       >  4:1179604/1‑147 (MQ=255)

                                                                                               ttGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATc                                                       >  4:330747/1‑147 (MQ=255)

                                                                                               ttGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGa                                                    <  3:217919/150‑1 (MQ=255)

                                                                                                 gTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGAttt                                                 <  1:1138823/151‑1 (MQ=255)

                                                                                                 gTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGAttt                                                 <  4:126814/151‑1 (MQ=255)

                                                                                                   cAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTcc                                               >  1:1454588/1‑151 (MQ=255)

                                                                                                   cAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTcc                                               >  1:695532/1‑151 (MQ=255)

                                                                                                   cAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTcc                                               >  4:1177926/1‑151 (MQ=255)

                                                                                                     aaaaTGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTccc                                              >  1:247802/1‑150 (MQ=255)

                                                                                                     aaaaTGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTccc                                              >  1:1081533/1‑150 (MQ=255)

                                                                                                      aaaTGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCa                                             >  4:969716/1‑150 (MQ=255)

                                                                                                       aaTGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCGGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAAc                                           <  1:1309386/151‑1 (MQ=255)

                                                                                                        aTGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACg                                          <  1:29071/151‑1 (MQ=255)

                                                                                                          gtgATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTg                                        <  1:922166/151‑1 (MQ=255)

                                                                                                           tgATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTg                                        <  1:320166/150‑1 (MQ=255)

                                                                                                             aTCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTa                                     <  3:458872/151‑1 (MQ=255)

                                                                                                                gATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTTGTAATc                                  <  3:40005/151‑1 (MQ=255)

                                                                                                                gATTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATc                                  >  4:130255/1‑151 (MQ=255)

                                                                                                                 aTTTTATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATc                                  >  1:1615941/1‑150 (MQ=255)

                                                                                                                   tttATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGc                               >  2:92404/1‑151 (MQ=255)

                                                                                                                    ttATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCg                              >  4:428918/1‑151 (MQ=255)

                                                                                                                     tATCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGt                             >  1:1280704/1‑151 (MQ=255)

                                                                                                                      aTCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTc                            >  1:1358622/1‑151 (MQ=255)

                                                                                                                       tCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTcg                           >  4:903606/1‑151 (MQ=255)

                                                                                                                       tCGGGTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTc                            >  3:89435/1‑150 (MQ=255)

                                                                                                                           gTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTCGCGAc                       >  1:1557510/1‑151 (MQ=255)

                                                                                                                           gTGCGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTCGCGAc                       >  4:1834998/1‑151 (MQ=255)

                                                                                                                              cGATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTCGCGACCTg                    >  4:593993/1‑151 (MQ=255)

                                                                                                                               gATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTCGCGACCTgg                   >  1:1568716/1‑151 (MQ=255)

                                                                                                                               gATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTCGCGACCTgg                   >  1:77704/1‑151 (MQ=255)

                                                                                                                               gATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTCGCGACCTgg                   >  2:373766/1‑151 (MQ=255)

                                                                                                                               gATCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTCGCGACCTgg                   >  4:1103435/1‑151 (MQ=255)

                                                                                                                                aTCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTCGCGACCTgg                   >  1:1269605/1‑150 (MQ=255)

                                                                                                                                aTCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTCGCGACCTGGt                  >  4:955455/1‑151 (MQ=255)

                                                                                                                                aTCCCCGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTCGCGACCTGGt                  >  1:1066840/1‑151 (MQ=255)

                                                                                                                                    ccGGTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTCGCGACCTGGTCCTc              >  4:1077489/1‑151 (MQ=255)

                                                                                                                                      ggTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTCGCGACCTGGTCCTCAc            >  2:342690/1‑151 (MQ=255)

                                                                                                                                       gTCCATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTCGCGACCTGGTCCTCACt           <  4:1591033/151‑1 (MQ=255)

                                                                                                                                         ccATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTCGCGACCTGGTCCTCACTg          <  4:833079/150‑1 (MQ=255)

                                                                                                                                         ccATCATCATTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTCGCGACCTGGTCCTCACTGc         >  1:1127235/1‑151 (MQ=255)

                                                                                                                                             catcatTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTCGCGACCTGGTCCTCACTGCCGCt     <  1:1805090/151‑1 (MQ=255)

                                                                                                                                                catTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTCGCGACCTGGTCCTCACTGCCGCTGt   <  4:228930/150‑1 (MQ=255)

                                                                                                                                                catTGACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTCGCGACCTGGTCCTCACTGCCGCTGTc  >  1:1529998/1‑151 (MQ=255)

CCGATTCCACGGCGATGCTGCCGCCGAGATTTTCTACCTGTTGTTTGACAAGTGCTAAACCGACGCCTCGCTCGCTTCCTTTTGTCGAGACACCTTTGTCAAAAATGTGATCGATTTTATCGGGTGCGATCCCCGGTCCATCATCATTAACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTCGCGACCTGGTCCTCACTGCCGCTGTC  >  NC_000913/4350096‑4350390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`