Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,493,603 T→C G268G (GGA→GGG)  idnD ← L‑idonate 5‑dehydrogenase, NAD‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,493,6030TC99.3% 504.6 / ‑4.5 139G268G (GGA→GGG) idnDL‑idonate 5‑dehydrogenase, NAD‑binding
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (62/76);  total (63/76)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

Only 100 of 140 total aligned reads displayed.

ATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATGTCGTCGTTTTGTGGGTTT  >  NC_000913/4493455‑4493750
                                                                                                                                                    |                                                                                                                                                   
atTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCcccc                                                                                                                                                   >  1:474176/1‑151 (MQ=255)
   cAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCAt                                                                                                                                                <  1:1681687/151‑1 (MQ=255)
    aGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCAt                                                                                                                                                >  4:1285661/1‑150 (MQ=255)
    aGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCAt                                                                                                                                                >  4:1397790/1‑150 (MQ=255)
       aCTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCAtacct                                                                                                                                            <  4:1175747/151‑1 (MQ=255)
          cAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCAtacctacc                                                                                                                                         >  4:491825/1‑151 (MQ=255)
           aGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCAtacctacc                                                                                                                                         <  3:82215/150‑1 (MQ=255)
           aGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATacctacct                                                                                                                                        <  1:1189161/151‑1 (MQ=255)
            gTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTg                                                                                                                                       <  1:1648645/151‑1 (MQ=255)
              aaaGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCa                                                                                                                                     <  1:594435/151‑1 (MQ=255)
              aaaGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCa                                                                                                                                     <  3:218758/151‑1 (MQ=255)
               aaGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCAc                                                                                                                                    >  1:1616664/1‑151 (MQ=255)
                   cAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCAtt                                                                                                                                <  4:1684869/151‑1 (MQ=255)
                     gTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTAc                                                                                                                              >  3:214716/1‑151 (MQ=255)
                      tgtaTTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTAcg                                                                                                                             >  3:347576/4‑151 (MQ=255)
                        gATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAGATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTAcgcc                                                                                                                           <  4:1034915/151‑1 (MQ=255)
                        gATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGGGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTAcgac                                                                                                                           >  2:118294/1‑149 (MQ=255)
                         aTTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTAcgccg                                                                                                                          >  1:976729/1‑151 (MQ=255)
                         aTTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTAcgccg                                                                                                                          >  1:903134/1‑151 (MQ=255)
                          ttGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTAcgccgc                                                                                                                         >  4:1474314/1‑151 (MQ=255)
                           tGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTAcgccgc                                                                                                                         <  1:1735727/150‑1 (MQ=255)
                            gATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCcgcg                                                                                                                        <  3:482708/150‑1 (MQ=255)
                             aTAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTg                                                                                                                      <  4:76313/151‑1 (MQ=255)
                             aTAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTg                                                                                                                      >  4:1171126/1‑151 (MQ=255)
                               aaCGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGc                                                                                                                     >  2:309053/1‑150 (MQ=255)
                               aaCGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGc                                                                                                                     <  1:557059/150‑1 (MQ=255)
                                 cGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACg                                                                                                                  <  4:187603/151‑1 (MQ=255)
                                   ccATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACg                                                                                                                  >  1:1408425/1‑149 (MQ=255)
                                     aTTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTg                                                                                                              >  4:1563558/1‑151 (MQ=255)
                                       tCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGAc                                                                                                            >  4:53715/1‑151 (MQ=255)
                                        cGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGAcc                                                                                                           >  4:1369911/1‑151 (MQ=255)
                                         gccagccaTGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCt                                                                                                          >  4:30574/1‑151 (MQ=255)
                                          ccagccaTGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTc                                                                                                         <  4:799784/151‑1 (MQ=255)
                                           cagccaTGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTcc                                                                                                        >  1:864056/1‑151 (MQ=255)
                                            agccaTGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCGCGAGTGACCTc                                                                                                         >  1:1016532/1‑149 (MQ=255)
                                             gccaTGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAg                                                                                                      <  2:377963/151‑1 (MQ=255)
                                               caTGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGAc                                                                                                    <  4:1076938/151‑1 (MQ=255)
                                                  gACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAgg                                                                                                 <  4:714974/151‑1 (MQ=255)
                                                  gACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAg                                                                                                  <  2:68457/150‑1 (MQ=255)
                                                          agTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACt                                                                                         >  4:1824187/2‑151 (MQ=255)
                                                             aTTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACtgat                                                                                      <  1:1264947/151‑1 (MQ=255)
                                                             aTTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACtga                                                                                       <  1:1394737/150‑1 (MQ=255)
                                                             aTTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACtga                                                                                       <  1:656333/150‑1 (MQ=255)
                                                                 aaTTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACtgatga                                                                                    >  4:1143489/1‑149 (MQ=255)
                                                                  aTTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTGCCTGCACCATTACGCCGCGTGCAAGAGTGACCTCCAGACAGGTATTCACTGATGAAgg                                                                                 <  1:1780172/151‑1 (MQ=255)
                                                                     cGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGAt                                                                               <  2:65484/150‑1 (MQ=255)
                                                                     cGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGAt                                                                               <  4:1740619/150‑1 (MQ=255)
                                                                     cGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATg                                                                              <  1:307856/151‑1 (MQ=255)
                                                                     cGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCCTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGa                                                                                <  1:171659/149‑1 (MQ=255)
                                                                           tAAAACGGAAAGAGCCTCTGAGTGAAATCCCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCgg                                                                         >  1:794763/1‑150 (MQ=255)
                                                                             aaaCGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGaca                                                                      <  3:51137/151‑1 (MQ=255)
                                                                             aaaCGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGac                                                                       >  2:474541/1‑150 (MQ=255)
                                                                              aaCGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCATCGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGacac                                                                     >  4:241537/1‑151 (MQ=255)
                                                                              aaCGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGacac                                                                     >  1:1492430/1‑151 (MQ=255)
                                                                               aCGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACt                                                                    <  3:35840/151‑1 (MQ=255)
                                                                               aCGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACt                                                                    <  2:111107/151‑1 (MQ=255)
                                                                                cGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACtt                                                                   <  4:694398/151‑1 (MQ=255)
                                                                                  gAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTc                                                                  >  1:1875477/1‑150 (MQ=255)
                                                                                  gAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTc                                                                  >  4:917633/1‑150 (MQ=255)
                                                                                   aaaGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCaa                                                                <  2:450404/151‑1 (MQ=255)
                                                                                    aaGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCaa                                                                >  4:449769/1‑150 (MQ=255)
                                                                                    aaGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCaaa                                                               <  1:208419/151‑1 (MQ=255)
                                                                                    aaGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCaaa                                                               <  4:1614022/151‑1 (MQ=255)
                                                                                    aaGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCaa                                                                >  4:1480826/1‑150 (MQ=255)
                                                                                    aaGAGCCTCTGAGTGAAATCACCTTACCAATCAACGTCAGCATTGGGAATTCTCCCATCGCGCCCCCCATACCGACCTAGACCGTTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGAGCGGCCACTTCaa                                                                <  1:342349/150‑1 (MQ=255)
                                                                                          ctctGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGAc                                                         >  1:1907630/1‑151 (MQ=255)
                                                                                           tctGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGAc                                                         >  4:1669454/1‑150 (MQ=255)
                                                                                              gAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACAt                                                       <  4:343457/149‑1 (MQ=255)
                                                                                                  gAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAAt                                                 <  4:1662668/151‑1 (MQ=255)
                                                                                                  gAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAAt                                                 <  4:986009/151‑1 (MQ=255)
                                                                                                  gAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAAt                                                 <  1:972465/151‑1 (MQ=255)
                                                                                                   aaaTCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATa                                                <  2:306858/151‑1 (MQ=255)
                                                                                                      tctcCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCGATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGaa                                                   <  3:465216/145‑1 (MQ=255)
                                                                                                      tctcCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCGATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGaa                                                   >  2:465232/1‑145 (MQ=255)
                                                                                                         ccTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCtt                                           <  4:1135522/150‑1 (MQ=255)
                                                                                                            tACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCttttt                                        <  1:1642730/150‑1 (MQ=255)
                                                                                                             aCCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTcc                                      >  4:1097356/1‑151 (MQ=255)
                                                                                                              ccAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTcc                                      <  1:112776/150‑1 (MQ=255)
                                                                                                              ccAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTcc                                      <  1:525587/150‑1 (MQ=255)
                                                                                                               cAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGc                                    >  1:104421/1‑151 (MQ=255)
                                                                                                                aaTCAACGTCATCATTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCt                                   <  1:1480853/151‑1 (MQ=255)
                                                                                                                        tcatcaTTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTg                           >  4:61991/1‑151 (MQ=255)
                                                                                                                            catTGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATcc                       <  2:38564/151‑1 (MQ=255)
                                                                                                                               tGGGAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCAt                     >  4:1851427/1‑150 (MQ=255)
                                                                                                                                 ggAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATgtc                  <  1:1820616/151‑1 (MQ=255)
                                                                                                                                 ggAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATgtc                  >  1:1149014/1‑151 (MQ=255)
                                                                                                                                 ggAATTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATgtc                  >  4:7739/1‑151 (MQ=255)
                                                                                                                                    aTTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATgtcgtc               <  1:425027/151‑1 (MQ=255)
                                                                                                                                    aTTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATgtcgtc               <  4:474351/151‑1 (MQ=255)
                                                                                                                                    aTTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATgtcgtc               >  3:215434/1‑151 (MQ=255)
                                                                                                                                    aTTCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATgtcgtc               <  4:807828/151‑1 (MQ=255)
                                                                                                                                     ttCTGCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATGtcgtcg              >  4:819066/1‑151 (MQ=255)
                                                                                                                                         gCCATCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATGTCGTCGtt            <  1:1045814/149‑1 (MQ=255)
                                                                                                                                             tCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATGTCGTCGTTTTGTgg      >  4:1156404/1‑151 (MQ=255)
                                                                                                                                             tCGCGCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATGTCGTCGTTTTGTgg      >  1:688154/1‑151 (MQ=255)
                                                                                                                                              cgcgCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATGTCGTCGTTTTGTggg     <  4:1016974/151‑1 (MQ=255)
                                                                                                                                              cgcgCCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATGTCGTCGTTTTGTgg      <  1:498895/150‑1 (MQ=255)
                                                                                                                                                 gcCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATGTCGTCGTTTTGTGGGttt  >  1:1111784/1‑151 (MQ=255)
                                                                                                                                                 gcCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATGTCGTCGTTTTGTGGGttt  <  4:72620/151‑1 (MQ=255)
                                                                                                                                                 gcCCCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATGTCGTCGTTTTGTGGGttt  >  2:16480/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATGTCGTCGTTTTGTGGGTTT  >  NC_000913/4493455‑4493750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑