BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,590,696	A→G	V195A (GTC→GCC)	yjiY ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,590,696	0	A	G	99.2%	456.9 / ‑4.5	126	V195A (GTC→GCC)	yjiY	putative transporter
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (67/58); total (67/59)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 127 total aligned reads displayed.

TCGTGAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCATCTCTTCTTTGATCATCTCACCAAGAGATGCG  >  NC_000913/4590549‑4590842

tCGTGAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGgcac                                                                                                                                                 >  1:143025/1‑151 (MQ=255)

   tGAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCAccc                                                                                                                                               <  1:1153843/150‑1 (MQ=255)

    gAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCAcccc                                                                                                                                              <  4:1469929/150‑1 (MQ=255)

    gAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCAcccc                                                                                                                                              >  1:1555920/1‑150 (MQ=255)

      gCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACg                                                                                                                                           >  1:915694/1‑151 (MQ=255)

       cAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACgg                                                                                                                                          >  2:325591/1‑151 (MQ=255)

           cACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACtt                                                                                                                                      <  1:1096604/151‑1 (MQ=255)

           cACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACt                                                                                                                                       <  2:227952/150‑1 (MQ=255)

              gCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCg                                                                                                                                   >  4:1103431/1‑151 (MQ=255)

              gCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCg                                                                                                                                   <  4:10392/151‑1 (MQ=255)

               ccACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCgg                                                                                                                                  <  4:396380/151‑1 (MQ=255)

                cACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGc                                                                                                                                 >  1:493150/1‑151 (MQ=255)

                    gAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAgg                                                                                                                             <  2:26662/151‑1 (MQ=255)

                      aGTAGATAGAGGCAACCAGCAGCACGATACCAATGCCAGAGCCTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAAGCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAgg                                                                                                                             <  1:756519/149‑1 (MQ=255)

                        tagatagaGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCtt                                                                                                                         >  2:535854/1‑151 (MQ=255)

                           atagaGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTaa                                                                                                                      <  1:1877517/151‑1 (MQ=255)

                           atagaGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTaa                                                                                                                      <  1:538540/151‑1 (MQ=255)

                           atagaGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTa                                                                                                                       >  4:1550615/1‑150 (MQ=255)

                               agGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACcac                                                                                                                  <  4:1557217/151‑1 (MQ=255)

                                ggCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACcaca                                                                                                                 <  4:1752969/151‑1 (MQ=255)

                                ggCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACcac                                                                                                                  >  4:370559/1‑150 (MQ=255)

                                   aaCCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATc                                                                                                              >  1:353793/1‑151 (MQ=255)

                                   aaCCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATc                                                                                                              >  1:364128/1‑151 (MQ=255)

                                       agcagcACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAgcg                                                                                                          >  4:488057/1‑151 (MQ=255)

                                           gcaCGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGcc                                                                                                        >  1:1347110/1‑149 (MQ=255)

                                              cGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGAc                                                                                                   <  1:247528/151‑1 (MQ=255)

                                              cGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGAc                                                                                                   <  4:1881503/151‑1 (MQ=255)

                                              cGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGAc                                                                                                   >  3:35555/1‑151 (MQ=255)

                                               gATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACg                                                                                                  >  2:8959/1‑151 (MQ=255)

                                               gATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACg                                                                                                  >  1:1526701/1‑151 (MQ=255)

                                                  aCCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGc                                                                                                <  1:269150/150‑1 (MQ=255)

                                                   ccAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGa                                                                                              <  4:1370094/151‑1 (MQ=255)

                                                    cAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCg                                                                                               >  4:1117117/1‑149 (MQ=255)

                                                    cAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAg                                                                                             <  4:1548872/151‑1 (MQ=255)

                                                        gACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGgatg                                                                                         >  4:1333359/1‑151 (MQ=255)

                                                          cAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGgatgat                                                                                       >  1:563253/1‑151 (MQ=255)

                                                          cAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGgatgat                                                                                       <  1:919983/151‑1 (MQ=255)

                                                          cAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGgatgat                                                                                       >  1:299909/1‑151 (MQ=255)

                                                           agagACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTAGGCCAGGGATTTAACCACAATCAGCGCCAGGACGGCGAGgatgatg                                                                                      >  3:417350/1‑151 (MQ=255)

                                                                cTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCa                                                                                  <  1:272970/150‑1 (MQ=255)

                                                                 ttCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATg                                                                                >  4:1086615/1‑151 (MQ=255)

                                                                  tCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCAt                                                                                 <  1:777783/149‑1 (MQ=255)

                                                                   cACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGAt                                                                              <  4:302554/151‑1 (MQ=255)

                                                                    aCCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGAtt                                                                             <  4:1438248/151‑1 (MQ=255)

                                                                    aCCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGAtt                                                                             >  4:353941/1‑151 (MQ=255)

                                                                      ccACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTaa                                                                           <  4:1501768/151‑1 (MQ=255)

                                                                      ccACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTaa                                                                           <  4:1634377/151‑1 (MQ=255)

                                                                      ccACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTa                                                                            >  4:282912/1‑150 (MQ=255)

                                                                      ccACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTaa                                                                           <  4:1317738/151‑1 (MQ=255)

                                                                        acacGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTa                                                                            >  2:319469/1‑148 (MQ=255)

                                                                        acacGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGa                                                                         <  4:928009/151‑1 (MQ=255)

                                                                         cacGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGa                                                                         <  4:437215/150‑1 (MQ=255)

                                                                         cacGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAAACACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGa                                                                         >  4:509369/1‑150 (MQ=255)

                                                                          acGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGaaa                                                                       <  4:456449/151‑1 (MQ=255)

                                                                          acGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGaa                                                                        >  1:1487989/1‑150 (MQ=255)

                                                                           cGCCCTGGACGAATAAAGCGCATGTAAATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGAGAAGGCACCCCACCGACTCTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGACGAGGTTGATGATCAGGATTAAGaaa                                                                       >  3:497536/1‑150 (MQ=255)

                                                                            gCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAAc                                                                      >  1:1621685/1‑150 (MQ=255)

                                                                             ccccGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAAc                                                                      >  1:83844/1‑149 (MQ=255)

                                                                              cccGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGc                                                                   >  4:325212/1‑151 (MQ=255)

                                                                                 ggACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCa                                                                 >  4:1754467/1‑150 (MQ=255)

                                                                                  gACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCaa                                                                >  4:1635482/1‑150 (MQ=255)

                                                                                    cGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAAc                                                              >  1:724672/1‑150 (MQ=255)

                                                                                    cGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACa                                                             <  3:463550/151‑1 (MQ=255)

                                                                                       aTAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAgcg                                                          >  2:368167/1‑151 (MQ=255)

                                                                                         aaaGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGcgcg                                                        <  4:1330128/151‑1 (MQ=255)

                                                                                          aaGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGa                                                       <  4:1881795/151‑1 (MQ=255)

                                                                                            gcgcATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATa                                                     <  1:909075/151‑1 (MQ=255)

                                                                                             cgcATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGAt                                                      >  1:1353814/1‑149 (MQ=255)

                                                                                             cgcATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGAt                                                      >  4:1029681/1‑149 (MQ=255)

                                                                                              gcATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGt                                                   >  1:774879/1‑151 (MQ=255)

                                                                                                     gATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGt                                            <  1:1777896/151‑1 (MQ=255)

                                                                                                       tACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTAc                                          >  2:148492/1‑151 (MQ=255)

                                                                                                        aCCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACt                                         <  2:262590/151‑1 (MQ=255)

                                                                                                         cccATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAAACAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACt                                         <  4:1695556/150‑1 (MQ=255)

                                                                                                               aaCAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTccc                                   >  1:1683983/1‑150 (MQ=255)

                                                                                                               aaCAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCa                                  <  1:1347534/151‑1 (MQ=255)

                                                                                                                aCAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCAt                                 >  1:1520121/1‑151 (MQ=255)

                                                                                                                aCAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCAt                                 <  1:80334/151‑1 (MQ=255)

                                                                                                                  aGCGCAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCAtct                               <  4:83868/151‑1 (MQ=255)

                                                                                                                   gcgcAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCAtct                               <  1:1626301/150‑1 (MQ=255)

                                                                                                                    cgcAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCATctct                             >  4:1772182/1‑151 (MQ=255)

                                                                                                                    cgcAATCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCATctct                             >  4:1915509/1‑151 (MQ=255)

                                                                                                                       aaTCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCATCtcttct                          <  2:341399/151‑1 (MQ=255)

                                                                                                                        aTCGGTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCATCTcttctt                         >  3:154934/1‑151 (MQ=255)

                                                                                                                           ggTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCATCTCTTCTTTGa                      <  1:757424/151‑1 (MQ=255)

                                                                                                                            gTACGGTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCATCTCTTCTTTGat                     <  3:452315/151‑1 (MQ=255)

                                                                                                                               aggTTGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCATCTCTTCTTTGatca                   >  1:690004/2‑150 (MQ=255)

                                                                                                                                  ttGAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGGACTGGTCCCATCTCTTCTTTGATCAtctc               >  3:194298/1‑151 (MQ=255)

                                                                                                                                    gAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCATCTCTTCTTTGATCATCTCa              <  2:493178/150‑1 (MQ=255)

                                                                                                                                    gAGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCATCTCTTCTTTGATCATCTCa              <  1:1201085/150‑1 (MQ=255)

                                                                                                                                     aGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCATCTCTTCTTTGATCATCTCAcc            >  4:1210394/1‑151 (MQ=255)

                                                                                                                                     aGCAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCATCTCTTCTTTGATCATCTCAcc            >  3:207732/1‑151 (MQ=255)

                                                                                                                                       cAAACGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCATCTCTTCTTTGATCATCTCACCaa          <  4:1883658/151‑1 (MQ=255)

                                                                                                                                           cGGTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCATCTCTTCTTTGATCATCTCACCAag         >  4:1502232/1‑148 (MQ=255)

                                                                                                                                            ggTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCATCTCTTCTTTGATCATCTCACCAag         >  4:1227108/1‑147 (MQ=255)

                                                                                                                                            ggTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCATCTCTTCTTTGATCATCTCACCAag         >  3:542900/1‑147 (MQ=255)

                                                                                                                                            ggTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCATCTCTTCTTTGATCATCTCACCAag         >  2:51136/1‑147 (MQ=255)

                                                                                                                                            ggTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCATCTCTTCTTTGATCATCTCACCAag         >  4:517721/1‑147 (MQ=255)

                                                                                                                                             gTGAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCATCTCTTCTTTGATCATCTCACCAAGAGATg    >  1:114560/1‑151 (MQ=255)

                                                                                                                                               gAAGGCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCATCTCTTCTTTGATCATCTCACCAAGAGATgcg  <  4:888875/151‑1 (MQ=255)

TCGTGAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAGTCCCCGGTACTGGTCCCATCTCTTCTTTGATCATCTCACCAAGAGATGCG  >  NC_000913/4590549‑4590842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`