Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,631,960 C→T P410S (CCG→TCG)  slt → lytic murein transglycosylase, soluble

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,631,9600CT100.0% 678.6 / NA 171P410S (CCG→TCG) sltlytic murein transglycosylase, soluble
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (82/89);  total (82/89)

Only 100 of 171 total aligned reads displayed.

TGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTA  >  NC_000913/4631815‑4632107
                                                                                                                                                 |                                                                                                                                                   
tgaagctgaagcAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCgg                                                                                                                                                  >  2:124596/1‑149 (MQ=255)
 gaagctgaagcAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCgg                                                                                                                                                  >  4:1335065/1‑148 (MQ=255)
  aagctgaagcAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCgg                                                                                                                                                  >  1:1543627/1‑147 (MQ=255)
  aagctgaagcAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGAt                                                                                                                                              <  1:1715006/151‑1 (MQ=255)
   agctgaagcAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATg                                                                                                                                             <  1:1825808/151‑1 (MQ=255)
     ctgaagcAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGgc                                                                                                                                           >  4:1295995/1‑151 (MQ=255)
       gaagcAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGgcg                                                                                                                                          <  2:233105/150‑1 (MQ=255)
       gaagcAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGgcg                                                                                                                                          <  2:35257/150‑1 (MQ=255)
         agcAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGgcgcgc                                                                                                                                       >  1:1176420/1‑151 (MQ=255)
          gcAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGcgcgcg                                                                                                                                      <  1:511721/151‑1 (MQ=255)
           cAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGt                                                                                                                                     >  1:836939/1‑151 (MQ=255)
            aaaaGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTg                                                                                                                                    <  4:822038/151‑1 (MQ=255)
             aaaGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTgc                                                                                                                                   >  4:1314427/1‑151 (MQ=255)
                gagaTTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGcgcg                                                                                                                                >  2:242383/1‑151 (MQ=255)
                 agaTTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGcgcg                                                                                                                                >  3:527567/1‑150 (MQ=255)
                 agaTTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGa                                                                                                                               <  4:406913/151‑1 (MQ=255)
                  gaTTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAg                                                                                                                              <  3:98443/151‑1 (MQ=255)
                   aTTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGt                                                                                                                             <  1:1835004/151‑1 (MQ=255)
                    ttttGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGtt                                                                                                                            >  1:1388029/1‑151 (MQ=255)
                     tttGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGtt                                                                                                                            <  3:43774/150‑1 (MQ=255)
                     tttGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCAGCAGAATGTTGACAGCGCCTTGCCTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGtt                                                                                                                            <  4:67671/150‑1 (MQ=255)
                       tGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGAt                                                                                                                         >  4:665305/1‑151 (MQ=255)
                        gCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATg                                                                                                                        <  2:59171/151‑1 (MQ=255)
                         cATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGt                                                                                                                       >  4:1260250/1‑151 (MQ=255)
                            cAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACt                                                                                                                    >  4:647530/1‑151 (MQ=255)
                            cAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACt                                                                                                                    >  3:346963/1‑151 (MQ=255)
                             aaCTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTg                                                                                                                   <  3:206797/151‑1 (MQ=255)
                             aaCTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTg                                                                                                                   <  4:1033447/151‑1 (MQ=255)
                               cTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTgg                                                                                                                  >  4:1007249/1‑150 (MQ=255)
                                tCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGa                                                                                                                 >  1:144208/1‑150 (MQ=255)
                                    gCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAAtctc                                                                                                            >  1:288375/1‑151 (MQ=255)
                                     cAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCg                                                                                                           <  1:129814/151‑1 (MQ=255)
                                      aaCAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCg                                                                                                           >  1:297217/1‑150 (MQ=255)
                                       aCAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGat                                                                                                         >  1:1001695/1‑151 (MQ=255)
                                         aGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGata                                                                                                        >  1:810785/1‑150 (MQ=255)
                                          gCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGataat                                                                                                      >  4:1537851/1‑151 (MQ=255)
                                           cGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGataata                                                                                                     <  1:1244081/151‑1 (MQ=255)
                                           cGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGataat                                                                                                      >  4:1721332/1‑150 (MQ=255)
                                             tGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAGGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATAcc                                                                                                   >  1:415711/1‑151 (MQ=255)
                                               gTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACcgc                                                                                                 >  1:1476346/1‑151 (MQ=255)
                                               gTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACcgc                                                                                                 <  4:142688/151‑1 (MQ=255)
                                               gTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACcg                                                                                                  >  4:480556/1‑150 (MQ=255)
                                                tttCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACcgcg                                                                                                >  1:432621/1‑151 (MQ=255)
                                                    tACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGt                                                                                             >  1:4752/1‑150 (MQ=255)
                                                    tACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGt                                                                                             >  1:1576578/1‑150 (MQ=255)
                                                     aCCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGATCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACcgcgcg                                                                                              <  2:432451/148‑1 (MQ=255)
                                                        cGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGa                                                                                        <  1:100851/151‑1 (MQ=255)
                                                          aTGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGt                                                                                      <  1:1640718/151‑1 (MQ=255)
                                                          aTGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGt                                                                                      <  4:1719544/151‑1 (MQ=255)
                                                               tGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGc                                                                                  <  4:982982/150‑1 (MQ=255)
                                                                 cagcaCAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCaa                                                                               <  4:141255/151‑1 (MQ=255)
                                                                    cacaACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCt                                                                            >  3:100148/1‑151 (MQ=255)
                                                                       aaCGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGt                                                                         >  1:287856/1‑151 (MQ=255)
                                                                        aCGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTg                                                                        >  3:26890/1‑151 (MQ=255)
                                                                         cGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTg                                                                        >  1:15978/1‑150 (MQ=255)
                                                                          gCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGaa                                                                      <  1:588001/151‑1 (MQ=255)
                                                                           cATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAag                                                                     <  4:498071/151‑1 (MQ=255)
                                                                            aTCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAaga                                                                    <  1:157082/151‑1 (MQ=255)
                                                                            aTCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAaga                                                                    <  2:100149/151‑1 (MQ=255)
                                                                              cGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGc                                                                  <  4:1129355/151‑1 (MQ=255)
                                                                              cGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGc                                                                  >  4:857823/1‑151 (MQ=255)
                                                                                gCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCaa                                                                <  1:769855/151‑1 (MQ=255)
                                                                                 cGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCa                                                                 >  1:502521/1‑149 (MQ=255)
                                                                                  gAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGt                                                              <  3:306400/151‑1 (MQ=255)
                                                                                    agagTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCa                                                            <  4:1573670/151‑1 (MQ=255)
                                                                                       gTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCaaaa                                                         <  4:270672/151‑1 (MQ=255)
                                                                                        tATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCaaaa                                                         >  3:69943/1‑150 (MQ=255)
                                                                                         aTGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCaaaaa                                                        <  2:301735/150‑1 (MQ=255)
                                                                                              cTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGc                                                  >  1:710143/1‑151 (MQ=255)
                                                                                              cTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGc                                                  >  1:1267971/1‑151 (MQ=255)
                                                                                                gAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGc                                                  >  1:1083920/1‑149 (MQ=255)
                                                                                                  aGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGc                                              <  1:1364873/151‑1 (MQ=255)
                                                                                                    aTTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTc                                            <  2:228173/151‑1 (MQ=255)
                                                                                                         tAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTg                                       >  3:486802/1‑151 (MQ=255)
                                                                                                            aGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGgcg                                    <  1:1887048/151‑1 (MQ=255)
                                                                                                            aGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGgcg                                    >  4:1467200/1‑151 (MQ=255)
                                                                                                                  gcAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTAt                              >  1:1703093/1‑151 (MQ=255)
                                                                                                                   cAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATg                             >  4:757990/1‑151 (MQ=255)
                                                                                                                     gAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCt                           <  4:187771/151‑1 (MQ=255)
                                                                                                                     gAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCt                           >  1:1157813/1‑151 (MQ=255)
                                                                                                                     gAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCt                           <  4:1762534/151‑1 (MQ=255)
                                                                                                                      aaTGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCtt                          <  4:941828/151‑1 (MQ=255)
                                                                                                                        tGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGTCTCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTc                        >  4:76256/1‑151 (MQ=255)
                                                                                                                            gACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGCTGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTcaac                     >  1:99881/1‑150 (MQ=255)
                                                                                                                             aCAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTcaacaa                   <  4:281430/151‑1 (MQ=255)
                                                                                                                              cAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCaacaac                  >  1:214049/1‑151 (MQ=255)
                                                                                                                              cAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCaacaac                  <  4:1673872/151‑1 (MQ=255)
                                                                                                                               aGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCaacaact                 <  4:1325612/151‑2 (MQ=255)
                                                                                                                               aGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAAcc                 <  4:1121073/151‑1 (MQ=255)
                                                                                                                               aGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGAAGGCTCAACTGGCGCGGTATGCTTTCAACAAcc                 >  1:1802677/1‑151 (MQ=255)
                                                                                                                                 cgcCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCaa               <  1:888702/151‑1 (MQ=255)
                                                                                                                                  gcCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAAt              >  1:453961/1‑151 (MQ=255)
                                                                                                                                       gACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAAtggtgg         >  4:463082/1‑151 (MQ=255)
                                                                                                                                         cTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTggg        >  1:752270/1‑150 (MQ=255)
                                                                                                                                           cAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGAt      <  2:351730/150‑1 (MQ=255)
                                                                                                                                           cAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATc     >  4:644636/1‑151 (MQ=255)
                                                                                                                                             ggggTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCtt   <  3:302974/151‑1 (MQ=255)
                                                                                                                                             ggggTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCtt   <  4:889308/151‑1 (MQ=255)
                                                                                                                                              gggTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTa  <  4:42029/151‑1 (MQ=255)
                                                                                                                                              gggTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTa  <  1:703383/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTA  >  NC_000913/4631815‑4632107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑