Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 450,945 A→G A222A (GCT→GCC)  cyoA ← cytochrome o ubiquinol oxidase subunit II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913450,9450AG100.0% 544.3 / NA 139A222A (GCT→GCC) cyoAcytochrome o ubiquinol oxidase subunit II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (73/66);  total (73/66)

Only 100 of 139 total aligned reads displayed.

GGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGACGCGGAATGAAGAA  >  NC_000913/450799‑451091
                                                                                                                                                  |                                                                                                                                                  
ggTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGc                                                                                                                                                  <  4:46942/149‑1 (MQ=255)
 gattCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCttt                                                                                                                                               >  1:1319443/3‑151 (MQ=255)
 gTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCttt                                                                                                                                               >  2:181008/1‑151 (MQ=255)
 gTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCttt                                                                                                                                               >  1:924367/1‑151 (MQ=255)
   ttCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTg                                                                                                                                              <  4:230771/150‑1 (MQ=255)
    tCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGa                                                                                                                                             <  4:981252/150‑1 (MQ=255)
    tCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGa                                                                                                                                             >  3:365884/1‑150 (MQ=255)
     cACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGaa                                                                                                                                            <  1:602180/150‑1 (MQ=255)
       cGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGaagt                                                                                                                                          <  1:739848/150‑3 (MQ=255)
        gTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTc                                                                                                                                        >  2:414673/1‑151 (MQ=255)
         ttGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTc                                                                                                                                        >  2:296213/1‑150 (MQ=255)
           ggAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCAt                                                                                                                                      <  3:233148/150‑1 (MQ=255)
            gagaAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGc                                                                                                                                    <  1:1758111/151‑1 (MQ=255)
                aaTATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTg                                                                                                                                 >  2:261458/1‑150 (MQ=255)
                  tatTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTg                                                                                                                                 >  3:488088/1‑148 (MQ=255)
                   atTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTgaga                                                                                                                              <  3:362168/150‑1 (MQ=255)
                      ccACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGcc                                                                                                                          <  1:1783921/151‑1 (MQ=255)
                       cACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGccc                                                                                                                         <  1:1685063/151‑1 (MQ=255)
                        aCCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCg                                                                                                                        <  4:1849754/151‑1 (MQ=255)
                           tGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGc                                                                                                                     >  4:1518706/1‑151 (MQ=255)
                           tGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGc                                                                                                                     >  1:230287/1‑151 (MQ=255)
                            ggTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCggg                                                                                                                      <  4:1031516/149‑1 (MQ=255)
                            ggTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGcc                                                                                                                    <  1:266023/151‑1 (MQ=255)
                              ttGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGc                                                                                                                  >  2:143056/1‑151 (MQ=255)
                                   ttCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGt                                                                                                               >  4:481148/1‑149 (MQ=255)
                                    tCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGc                                                                                                            >  1:756255/1‑151 (MQ=255)
                                     cGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGc                                                                                                            <  2:100544/150‑1 (MQ=255)
                                     cGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCACGCCTGAGAAGCCCGGGCCGCTGTAGc                                                                                                            >  1:755641/1‑150 (MQ=255)
                                       cTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTgg                                                                                                         >  1:126803/1‑151 (MQ=255)
                                        tAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGc                                                                                                        >  3:345175/1‑151 (MQ=255)
                                         aGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCg                                                                                                       >  1:1907972/1‑151 (MQ=255)
                                              cgGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGAt                                                                                                  <  4:324984/151‑1 (MQ=255)
                                               ggCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATa                                                                                                 <  4:1437014/151‑1 (MQ=255)
                                                gCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATAc                                                                                                >  4:1421109/1‑151 (MQ=255)
                                                 ccAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGGATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATAcc                                                                                               >  1:157003/1‑151 (MQ=255)
                                                 ccAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATAcc                                                                                               >  4:795133/1‑151 (MQ=255)
                                                       tttCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCAt                                                                                          <  4:1850909/150‑1 (MQ=255)
                                                         tCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATa                                                                                         >  4:590374/1‑149 (MQ=255)
                                                         tCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATa                                                                                         >  4:1338847/1‑149 (MQ=255)
                                                            aaCGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGc                                                                                    >  2:537938/1‑151 (MQ=255)
                                                              cGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCg                                                                                  <  4:1394577/151‑1 (MQ=255)
                                                               gCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCgg                                                                                 >  4:215606/1‑151 (MQ=255)
                                                                cAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCggg                                                                                >  1:624646/1‑151 (MQ=255)
                                                                 aGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGt                                                                               <  3:194259/151‑1 (MQ=255)
                                                                  gCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGtt                                                                              <  4:70997/151‑1 (MQ=255)
                                                                  gCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGt                                                                               <  1:1240056/150‑1 (MQ=255)
                                                                   ccATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTc                                                                             <  3:313711/151‑1 (MQ=255)
                                                                    cATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCg                                                                            <  4:1600172/151‑1 (MQ=255)
                                                                      tGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGtt                                                                          >  4:1266514/1‑151 (MQ=255)
                                                                       gTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGtt                                                                          <  4:602513/150‑1 (MQ=255)
                                                                        tCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTgg                                                                        >  1:1667327/1‑151 (MQ=255)
                                                                          aGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCg                                                                      <  4:1062917/151‑1 (MQ=255)
                                                                            aCATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGAt                                                                    >  1:1415298/1‑151 (MQ=255)
                                                                              aTGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCa                                                                  <  4:1608077/151‑1 (MQ=255)
                                                                               tGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGATGGCGGAGATACCGTCATAAGTGCCGGGCTCGTTGGCGATc                                                                   >  4:781492/1‑149 (MQ=255)
                                                                                ggTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAg                                                                 <  3:6878/150‑1 (MQ=255)
                                                                                ggTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAg                                                                 <  4:1747500/150‑1 (MQ=255)
                                                                                ggTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGa                                                                <  4:692965/151‑1 (MQ=255)
                                                                                   gtTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATg                                                              <  1:1520517/150‑1 (MQ=255)
                                                                                    ttCGGGGACTGCTTCGCTTTTGCGACCCCCTGGTCGAATGCGGCGCGATCGGGTGTTGCAATGGCTTTGAACGTCATGACTGAGAAGCCCGGGCCGTTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGAt                                                               <  4:209272/148‑1 (MQ=255)
                                                                                     tCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCa                                                            <  4:363370/150‑1 (MQ=255)
                                                                                         cGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCg                                                        >  4:354437/1‑150 (MQ=255)
                                                                                            cTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGt                                                     <  4:636259/150‑1 (MQ=255)
                                                                                              gCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCt                                                   <  1:199172/150‑1 (MQ=255)
                                                                                                  cGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCAt                                               <  1:970788/150‑1 (MQ=255)
                                                                                                  cGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCAt                                               >  1:1514354/1‑150 (MQ=255)
                                                                                                   gCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATAc                                             >  4:1265998/1‑151 (MQ=255)
                                                                                                   gCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATAc                                             >  4:759642/1‑151 (MQ=255)
                                                                                                     ttttGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCg                                           >  4:1490120/1‑151 (MQ=255)
                                                                                                     ttttGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCg                                           >  3:5195/1‑151 (MQ=255)
                                                                                                      tttGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCg                                           <  3:525223/150‑1 (MQ=255)
                                                                                                        tGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGcc                                        >  1:1076569/1‑151 (MQ=255)
                                                                                                        tGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGcc                                        >  1:1129676/1‑151 (MQ=255)
                                                                                                         gCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGcc                                        >  2:379577/1‑150 (MQ=255)
                                                                                                         gCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGcc                                        >  2:335187/1‑150 (MQ=255)
                                                                                                          cGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCAt                                      >  1:892429/1‑151 (MQ=255)
                                                                                                                 cTGGTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATa                                >  1:1701537/1‑150 (MQ=255)
                                                                                                                   ggTCGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAAt                             >  4:1598048/1‑151 (MQ=255)
                                                                                                                      cGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCt                           <  4:575801/150‑1 (MQ=255)
                                                                                                                      cGAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTg                          >  4:652343/1‑151 (MQ=255)
                                                                                                                       gAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTgg                         <  1:38274/151‑1 (MQ=255)
                                                                                                                       gAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTgg                         >  3:332466/1‑151 (MQ=255)
                                                                                                                       gAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTgg                         >  1:1438446/1‑151 (MQ=255)
                                                                                                                       gAATGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTgg                         >  2:59523/1‑151 (MQ=255)
                                                                                                                         aTGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCt                       >  1:1099626/1‑151 (MQ=255)
                                                                                                                         aTGCGGCGCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCt                       <  1:1541103/151‑1 (MQ=255)
                                                                                                                             ggcgCGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTAccc                   <  4:1516233/151‑1 (MQ=255)
                                                                                                                              gcgcGATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCa                  <  4:1255073/151‑1 (MQ=255)
                                                                                                                                gcgATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGa                <  4:963948/151‑1 (MQ=255)
                                                                                                                                 cgATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGAc               >  1:482110/1‑151 (MQ=255)
                                                                                                                                  gATCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGAcg              >  3:185681/1‑151 (MQ=255)
                                                                                                                                    tCCGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGAcgcg            >  3:48229/1‑151 (MQ=255)
                                                                                                                                     ccGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGACGCgg           <  1:997911/151‑1 (MQ=255)
                                                                                                                                     ccGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGACGCgg           <  1:1556227/151‑1 (MQ=255)
                                                                                                                                     ccGGTGTTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGACGCgg           >  4:876356/1‑151 (MQ=255)
                                                                                                                                        gtgtTGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGACGCGGAAt        <  4:1643428/151‑1 (MQ=255)
                                                                                                                                           ttGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGACGCGGAATga      >  2:403225/1‑150 (MQ=255)
                                                                                                                                            tGCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGACGCGGAATga      >  4:690050/1‑149 (MQ=255)
                                                                                                                                             gCAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGACGCGGAATgaaga   <  4:1328561/151‑1 (MQ=255)
                                                                                                                                              cAATGGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGACGCGGAATgaagaa  <  4:867546/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGACGCGGAATGAAGAA  >  NC_000913/450799‑451091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑