Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 483,985 A→G V140A (GTC→GCC)  acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913483,9850AG100.0% 506.1 / NA 135V140A (GTC→GCC) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (69/66);  total (69/66)

Only 100 of 135 total aligned reads displayed.

TCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGG  >  NC_000913/483839‑484132
                                                                                                                                                  |                                                                                                                                                   
tCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAAc                                                                                                                                                 >  4:1793659/1‑151 (MQ=255)
tCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAAc                                                                                                                                                 >  1:1774588/1‑151 (MQ=255)
 cATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAAcc                                                                                                                                                >  4:416999/1‑151 (MQ=255)
  aTCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCa                                                                                                                                               <  1:403517/151‑1 (MQ=255)
   tCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCa                                                                                                                                               <  1:1672446/150‑1 (MQ=255)
     cAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATc                                                                                                                                             >  4:860172/1‑150 (MQ=255)
       gATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAgg                                                                                                                                          >  4:1849147/1‑151 (MQ=255)
         tACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGa                                                                                                                                         >  1:701138/1‑150 (MQ=255)
          aCGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTTATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGaa                                                                                                                                        <  2:92327/150‑1 (MQ=255)
           cGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAgc                                                                                                                                      <  1:1794318/151‑1 (MQ=255)
            gCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAgct                                                                                                                                     >  2:44376/1‑151 (MQ=255)
             cATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAgctg                                                                                                                                    <  4:487184/151‑1 (MQ=255)
              aTCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAgctgc                                                                                                                                   >  4:1172541/1‑151 (MQ=255)
               tCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAgctgct                                                                                                                                  >  1:837723/1‑151 (MQ=255)
                cgcgTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAgctgct                                                                                                                                  <  2:41092/150‑1 (MQ=255)
                 gcgTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTgg                                                                                                                                >  2:514573/1‑151 (MQ=255)
                  cgTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTgg                                                                                                                                >  1:859268/1‑150 (MQ=255)
                  cgTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGga                                                                                                                               <  4:379223/151‑1 (MQ=255)
                   gTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGgat                                                                                                                              >  1:118144/1‑151 (MQ=255)
                    tACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGgatg                                                                                                                             >  1:987789/1‑151 (MQ=255)
                     aCTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGgatg                                                                                                                             >  4:1863752/1‑150 (MQ=255)
                       tgtgAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGAtt                                                                                                                          >  1:205484/1‑151 (MQ=255)
                       tgtgAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGAtt                                                                                                                          >  4:780534/1‑151 (MQ=255)
                        gtgAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGAttt                                                                                                                         <  1:1455314/151‑1 (MQ=255)
                          gAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTtct                                                                                                                       >  2:126570/1‑151 (MQ=255)
                          gAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTtct                                                                                                                       <  1:1520208/151‑1 (MQ=255)
                          gAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTtc                                                                                                                        >  1:806851/1‑150 (MQ=255)
                          gAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTtc                                                                                                                        <  1:29242/150‑1 (MQ=255)
                           aaCCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTtctc                                                                                                                      >  2:327983/1‑151 (MQ=255)
                           aaCCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTtctc                                                                                                                      >  2:432992/1‑151 (MQ=255)
                               gAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACg                                                                                                                  <  1:986025/151‑1 (MQ=255)
                                  caacTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTc                                                                                                               <  1:1539374/151‑1 (MQ=255)
                                      tGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCAccc                                                                                                           >  2:304105/1‑151 (MQ=255)
                                      tGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCAccc                                                                                                           >  4:20685/1‑151 (MQ=255)
                                       gAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCAccc                                                                                                           <  4:1071885/150‑1 (MQ=255)
                                        aaCATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCt                                                                                                         <  1:939845/151‑1 (MQ=255)
                                         aCATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCtt                                                                                                        <  1:1526914/151‑1 (MQ=255)
                                          cATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTtg                                                                                                       >  4:1682184/1‑151 (MQ=255)
                                          cATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTtg                                                                                                       >  3:388463/1‑151 (MQ=255)
                                           aTCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTtgc                                                                                                      >  2:67722/1‑151 (MQ=255)
                                           aTCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTtgc                                                                                                      >  4:823325/1‑151 (MQ=255)
                                                ccACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGctgctg                                                                                                 <  3:542766/151‑1 (MQ=255)
                                                 cACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGa                                                                                                <  3:487678/151‑1 (MQ=255)
                                                   cGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAAc                                                                                              >  1:410286/1‑151 (MQ=255)
                                                     cccGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAActt                                                                                            <  4:1277463/151‑1 (MQ=255)
                                                     cccGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAActt                                                                                            >  4:1502562/1‑151 (MQ=255)
                                                          cGTACTGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAActtct                                                                                          <  1:1079416/148‑1 (MQ=255)
                                                            tACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCgg                                                                                     >  4:1687914/1‑151 (MQ=255)
                                                                 cTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGgcagc                                                                                 >  4:569345/1‑150 (MQ=255)
                                                                   gATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCaa                                                                               <  1:595553/150‑1 (MQ=255)
                                                                    aTGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACg                                                                             <  1:411566/151‑1 (MQ=255)
                                                                       gCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCa                                                                          <  4:25795/151‑1 (MQ=255)
                                                                           cTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGc                                                                      >  4:1116838/1‑151 (MQ=255)
                                                                            tttCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGcc                                                                     >  4:1849773/1‑151 (MQ=255)
                                                                             ttCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATc                                                                        <  1:1759363/147‑1 (MQ=255)
                                                                                 tatTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTg                                                                >  4:1567161/1‑151 (MQ=255)
                                                                                  atTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGc                                                               <  1:524798/151‑1 (MQ=255)
                                                                                   ttCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGc                                                               >  4:209999/1‑150 (MQ=255)
                                                                                    tCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGc                                                               >  1:510050/1‑149 (MQ=255)
                                                                                    tCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCa                                                              <  4:473210/150‑1 (MQ=255)
                                                                                     cgccgcCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGt                                                            >  1:1066493/1‑151 (MQ=255)
                                                                                     cgccgcCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGt                                                            <  4:1484210/151‑1 (MQ=255)
                                                                                      gccgccACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGtt                                                           <  1:193218/151‑1 (MQ=255)
                                                                                            aCGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCt                                                     <  4:1141868/151‑1 (MQ=255)
                                                                                              gTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCGTCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGc                                                   >  4:699549/1‑151 (MQ=255)
                                                                                              gTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGc                                                   <  4:330496/151‑1 (MQ=255)
                                                                                              gTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACAGCATCGCCAGCTGCAGTTTGTTCTGc                                                   >  2:507176/1‑151 (MQ=255)
                                                                                               tAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGc                                                   >  4:71525/1‑150 (MQ=255)
                                                                                                 gTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCAcc                                                <  1:57338/151‑1 (MQ=255)
                                                                                                     gagaTATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGaa                                            <  1:195218/151‑1 (MQ=255)
                                                                                                       gaTATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAAcc                                          <  4:1139500/151‑1 (MQ=255)
                                                                                                        atatCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCt                                         <  4:1775281/151‑1 (MQ=255)
                                                                                                         tatCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTg                                        >  1:555562/1‑151 (MQ=255)
                                                                                                          atCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgc                                       <  4:508719/151‑1 (MQ=255)
                                                                                                          atCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgc                                       >  1:26760/1‑151 (MQ=255)
                                                                                                                 tGCGTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGatat                                >  3:207261/1‑151 (MQ=255)
                                                                                                                    gTCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCg                             <  3:388070/151‑1 (MQ=255)
                                                                                                                     tCATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGATGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGc                            >  1:1840310/1‑151 (MQ=255)
                                                                                                                      cATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGc                            >  4:1014491/1‑150 (MQ=255)
                                                                                                                      cATGGTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGc                            <  3:70446/150‑1 (MQ=255)
                                                                                                                         ggTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCa                        <  2:360945/151‑1 (MQ=255)
                                                                                                                         ggTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCa                        <  1:698491/151‑1 (MQ=255)
                                                                                                                         ggTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCa                        <  1:997209/151‑1 (MQ=255)
                                                                                                                          gTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAg                       <  4:118178/151‑1 (MQ=255)
                                                                                                                          gTGCCATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAg                       <  1:1427574/151‑1 (MQ=255)
                                                                                                                             ccATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc                    >  4:1715474/1‑151 (MQ=255)
                                                                                                                              cATCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAcc                   >  4:827333/1‑151 (MQ=255)
                                                                                                                               aTCGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCa                  <  1:665484/151‑1 (MQ=255)
                                                                                                                                 cGGTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAcc                   >  1:1339533/1‑148 (MQ=255)
                                                                                                                                  ggTGTTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGAc               >  1:786200/1‑151 (MQ=255)
                                                                                                                                   gtgtTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACt              <  1:178417/151‑1 (MQ=255)
                                                                                                                                    tgtTGATAACGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAGCAGTACCAGACt              <  2:193286/150‑1 (MQ=255)
                                                                                                                                           aaCGCCGGCAACCATCAGGAATCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTc      <  1:40690/151‑1 (MQ=255)
                                                                                                                                           aaCGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTc      >  4:1423029/1‑151 (MQ=255)
                                                                                                                                            aCGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAgg        >  1:1118471/1‑148 (MQ=255)
                                                                                                                                             cGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAg    <  4:988215/151‑1 (MQ=255)
                                                                                                                                             cGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAg    <  2:385233/151‑1 (MQ=255)
                                                                                                                                             cGCCGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAg    <  2:190785/151‑1 (MQ=255)
                                                                                                                                               ccGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAggg  <  1:1173722/151‑1 (MQ=255)
                                                                                                                                               ccGGCAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAggg  <  1:1258510/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGG  >  NC_000913/483839‑484132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑