Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 535,299 T→C F462L (TTC→CTC)  gcl → glyoxylate carboligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913535,2990TC100.0% 593.1 / NA 156F462L (TTC→CTC) gclglyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (78/78);  total (78/78)

Only 100 of 156 total aligned reads displayed.

GATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTG  >  NC_000913/535151‑535443
                                                                                                                                                    |                                                                                                                                                
gATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTc                                                                                                                                                <  1:648345/151‑1 (MQ=255)
gATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTc                                                                                                                                                <  4:662931/151‑1 (MQ=255)
gATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTc                                                                                                                                                <  4:1894316/151‑1 (MQ=255)
  tCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCa                                                                                                                                               >  2:320124/1‑150 (MQ=255)
   cAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCaa                                                                                                                                              <  4:670044/150‑1 (MQ=255)
     aCTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAAGAt                                                                                                                                           <  1:254165/151‑1 (MQ=255)
     aCTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACAt                                                                                                                                           >  1:517753/1‑151 (MQ=255)
        gtgGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTcc                                                                                                                                        >  2:357854/1‑151 (MQ=255)
        gtgGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTc                                                                                                                                         <  2:527074/150‑1 (MQ=255)
              aGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACAt                                                                                                                                  >  4:764507/1‑151 (MQ=255)
               ggCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATc                                                                                                                                 <  4:659178/151‑1 (MQ=255)
                    gTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCAtgt                                                                                                                            >  1:1765783/1‑151 (MQ=255)
                    gTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCAtgt                                                                                                                            <  4:471346/151‑1 (MQ=255)
                     tCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCAtgtg                                                                                                                           >  2:192761/1‑151 (MQ=255)
                       cGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCAtgtg                                                                                                                           <  1:797632/149‑1 (MQ=255)
                        gTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTg                                                                                                                        >  3:105773/1‑151 (MQ=255)
                          tAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTgg                                                                                                                       <  4:1293413/150‑1 (MQ=255)
                               tGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTcaac                                                                                                                  >  4:1071311/1‑150 (MQ=255)
                                ggACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTcaacaa                                                                                                                <  2:289769/151‑1 (MQ=255)
                                     aTTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCtt                                                                                                           <  2:337506/151‑1 (MQ=255)
                                      ttCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCtt                                                                                                           >  4:791118/1‑150 (MQ=255)
                                        ccGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTAt                                                                                                         <  4:1791972/150‑1 (MQ=255)
                                         cGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCt                                                                                                       >  4:1671815/1‑151 (MQ=255)
                                         cGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCt                                                                                                       <  2:362779/151‑1 (MQ=255)
                                          ggCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTg                                                                                                      <  4:1863117/151‑1 (MQ=255)
                                             tGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTgggg                                                                                                   >  2:240640/1‑151 (MQ=255)
                                               cgcTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCt                                                                                                 <  1:832336/151‑1 (MQ=255)
                                                   aGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGAt                                                                                              <  3:338627/150‑1 (MQ=255)
                                                     gggTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCg                                                                                           >  4:53253/1‑151 (MQ=255)
                                                         ttGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGATGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCgtca                                                                                        <  4:161176/150‑1 (MQ=255)
                                                           gtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCgtcagtc                                                                                     >  4:852236/1‑151 (MQ=255)
                                                           gtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCgtcagt                                                                                      <  3:120048/150‑1 (MQ=255)
                                                              ccGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTcaca                                                                                  <  2:158318/151‑1 (MQ=255)
                                                                 cTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAAcg                                                                               >  1:753064/1‑151 (MQ=255)
                                                                 cTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAAcg                                                                               >  4:1474196/1‑151 (MQ=255)
                                                                  tGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAAcgc                                                                              >  1:891165/1‑151 (MQ=255)
                                                                   gATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAAcgcg                                                                             <  3:268985/151‑1 (MQ=255)
                                                                   gATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAAcgc                                                                              <  1:1081641/150‑1 (MQ=255)
                                                                      ccGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCtt                                                                          <  4:1294425/151‑1 (MQ=255)
                                                                      ccGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCtt                                                                          >  4:90832/1‑151 (MQ=255)
                                                                      ccGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCtg                                                                          <  1:1828372/151‑2 (MQ=255)
                                                                       cGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCttt                                                                         >  4:1094108/1‑151 (MQ=255)
                                                                        gAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCtttt                                                                        >  1:1372698/1‑151 (MQ=255)
                                                                         aaaCGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTg                                                                       <  4:718100/151‑1 (MQ=255)
                                                                         aaaCGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTg                                                                       >  1:1626076/1‑151 (MQ=255)
                                                                         aaaCGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTg                                                                       >  1:491691/1‑151 (MQ=255)
                                                                           aCGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGAc                                                                     >  4:4385/1‑151 (MQ=255)
                                                                            cGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGAc                                                                     >  1:1590640/1‑150 (MQ=255)
                                                                            cGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGAc                                                                     >  1:192546/1‑150 (MQ=255)
                                                                              cAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATg                                                                  <  1:110321/151‑1 (MQ=255)
                                                                                 tgtgGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGac                                                               >  1:1220308/1‑151 (MQ=255)
                                                                                  gtggtgGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGact                                                              >  4:829558/1‑151 (MQ=255)
                                                                                  gtggtgGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGact                                                              <  4:1719432/151‑1 (MQ=255)
                                                                                     gtggCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGactact                                                           <  2:105778/151‑1 (MQ=255)
                                                                                      tggCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTg                                                          >  1:1549657/1‑151 (MQ=255)
                                                                                       ggCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGc                                                         <  1:1650872/151‑1 (MQ=255)
                                                                                              tCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAAc                                                  >  1:1212018/1‑151 (MQ=255)
                                                                                              tCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAAc                                                  >  4:11747/1‑151 (MQ=255)
                                                                                                 ggCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTc                                                <  4:305934/150‑1 (MQ=255)
                                                                                                    gACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGc                                              <  4:563852/149‑1 (MQ=255)
                                                                                                    gACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCtt                                            >  1:1132454/1‑151 (MQ=255)
                                                                                                    gACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCtt                                            >  3:537352/1‑151 (MQ=255)
                                                                                                        ttGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTc                                          <  1:1927113/149‑1 (MQ=255)
                                                                                                        ttGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCg                                         >  1:1017177/1‑150 (MQ=255)
                                                                                                        ttGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCg                                         <  1:1791735/150‑1 (MQ=255)
                                                                                                        ttGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCg                                         <  1:1820275/150‑1 (MQ=255)
                                                                                                          gACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCgaga                                      <  2:265673/151‑1 (MQ=255)
                                                                                                          gACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCgag                                       <  4:526870/150‑1 (MQ=255)
                                                                                                          gACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCg                                         >  1:1388809/1‑148 (MQ=255)
                                                                                                           aCTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCgaga                                      >  4:1520418/1‑150 (MQ=255)
                                                                                                            cTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGaa                                     <  1:1279232/150‑1 (MQ=255)
                                                                                                            cTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAat                                    >  3:376674/1‑151 (MQ=255)
                                                                                                              tCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAata                                   <  2:290172/150‑1 (MQ=255)
                                                                                                               ccAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAatat                                  <  1:964587/150‑1 (MQ=255)
                                                                                                               ccAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATc                                 >  1:901607/1‑151 (MQ=255)
                                                                                                                   ttCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACt                             >  1:828158/1‑151 (MQ=255)
                                                                                                                     ccTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTcc                           >  4:354611/1‑151 (MQ=255)
                                                                                                                      cTGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTcc                           >  1:1067486/1‑150 (MQ=255)
                                                                                                                       tGATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTcc                           >  3:350199/1‑149 (MQ=255)
                                                                                                                        gATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCagt                        >  4:62139/1‑151 (MQ=255)
                                                                                                                        gATTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCa                          <  4:319360/149‑1 (MQ=255)
                                                                                                                         aTTGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCagtg                       <  4:1110309/151‑1 (MQ=255)
                                                                                                                           tGAAGAGTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCagtga                      >  2:249195/1‑150 (MQ=255)
                                                                                                                              agagTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCagtgaagtg                  >  4:112574/1‑151 (MQ=255)
                                                                                                                                agTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCagtgaagtga                 >  1:1683665/1‑150 (MQ=255)
                                                                                                                                agTTAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAgtgaagtgaa                <  1:53239/151‑1 (MQ=255)
                                                                                                                                  ttAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATg              >  1:1485908/1‑151 (MQ=255)
                                                                                                                                  ttAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATg              >  4:1327215/1‑151 (MQ=255)
                                                                                                                                  ttAGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATg              >  3:269040/1‑151 (MQ=255)
                                                                                                                                    aGCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATgg             <  4:1766476/150‑1 (MQ=255)
                                                                                                                                     gCTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCt           >  1:73007/1‑151 (MQ=255)
                                                                                                                                      cTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCt           >  1:712731/1‑150 (MQ=255)
                                                                                                                                      cTGTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCt           >  4:855680/1‑150 (MQ=255)
                                                                                                                                        gTTGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACg        <  2:241597/151‑1 (MQ=255)
                                                                                                                                         ttGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACgg       >  2:152278/1‑151 (MQ=255)
                                                                                                                                          tGGCGCGCAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACgg       >  1:569820/1‑150 (MQ=255)
                                                                                                                                            gcgcgcAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGgtgt    <  3:9513/151‑1 (MQ=255)
                                                                                                                                             cgcgcAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGtt   >  4:1353075/1‑151 (MQ=255)
                                                                                                                                             cgcgcAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGtt   <  1:1018910/151‑1 (MQ=255)
                                                                                                                                              gcgcAGCTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTg  <  1:1789432/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTG  >  NC_000913/535151‑535443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: