Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 553,403 C→T V180I (GTC→ATC)  lpxH ← UDP‑2,3‑diacylglucosamine pyrophosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913553,4030CT98.3% 454.5 / ‑2.2 118V180I (GTC→ATC) lpxHUDP‑2,3‑diacylglucosamine pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (60/56);  total (61/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

Only 100 of 121 total aligned reads displayed.

GTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACC  >  NC_000913/553255‑553548
                                                                                                                                                    |                                                                                                                                                 
  gACTTTCACCATTGAACCTTCCGTATGCCAGGCTCCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGatcac                                                                                                                                                <  1:1466638/150‑1 (MQ=255)
   aCTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACcg                                                                                                                                              <  1:451380/151‑1 (MQ=255)
    cTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACcgc                                                                                                                                             >  4:232002/1‑151 (MQ=255)
      ttCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGt                                                                                                                                           >  3:315880/1‑151 (MQ=255)
       tCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGtt                                                                                                                                          >  4:1581622/1‑151 (MQ=255)
        cACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGttt                                                                                                                                         <  2:419369/151‑1 (MQ=255)
            aTTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGTGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTgg                                                                                                                                      <  4:1434278/150‑1 (MQ=255)
            aTTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGt                                                                                                                                     >  4:1353777/1‑151 (MQ=255)
               gAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTaa                                                                                                                                  <  1:1609866/151‑1 (MQ=255)
                aaCCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAAc                                                                                                                                 >  4:1347341/1‑151 (MQ=255)
                  ccTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGt                                                                                                                               >  1:1391890/1‑151 (MQ=255)
                     tCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTcc                                                                                                                             <  1:1083314/150‑1 (MQ=255)
                     tCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTcc                                                                                                                             <  1:1228481/150‑1 (MQ=255)
                      ccGTATGCCAGGCCCCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCAt                                                                                                                           <  1:1481676/151‑1 (MQ=255)
                      ccGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCAt                                                                                                                           >  2:465740/1‑151 (MQ=255)
                       cGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTc                                                                                                                              <  4:323662/147‑1 (MQ=255)
                            gCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCg                                                                                                                      <  1:1237225/150‑1 (MQ=255)
                                  cACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCg                                                                                                                >  4:34086/1‑150 (MQ=255)
                                    cccAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAtt                                                                                                             <  1:1385494/151‑1 (MQ=255)
                                     ccAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAttt                                                                                                            >  2:69480/1‑151 (MQ=255)
                                      cAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAttt                                                                                                            >  4:496646/1‑150 (MQ=255)
                                      cAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTa                                                                                                           <  4:485677/151‑1 (MQ=255)
                                       aGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTAc                                                                                                          >  4:1203746/1‑151 (MQ=255)
                                         tACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTActg                                                                                                        >  4:1116362/1‑151 (MQ=255)
                                               gcgAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTg                                                                                                  <  1:1269297/151‑1 (MQ=255)
                                                cgAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTgg                                                                                                 >  3:164531/1‑151 (MQ=255)
                                                cgAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGCTAACGTCCATGATCGCCAGCGATTTACTGCTGTTgg                                                                                                 >  4:1469884/1‑151 (MQ=255)
                                                      gaatgTTGCTGGTTGGCGCTAAGTTCATGCACCGCCGGGCGATGGGGATGACCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCAATGATCGCCAGCGATCTACTGCTGTCAGcttct                                                                                            >  1:328741/5‑150 (MQ=255)
                                                       cAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCttt                                                                                          >  3:250373/1‑151 (MQ=255)
                                                       cAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCttt                                                                                          <  2:510478/151‑1 (MQ=255)
                                                        aGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTg                                                                                         >  3:49868/1‑151 (MQ=255)
                                                        aGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTg                                                                                         >  1:1315544/1‑151 (MQ=255)
                                                        aGGTTGCTGATTGGCGATAAGCTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTg                                                                                         <  4:741315/151‑1 (MQ=255)
                                                                gATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTcgc                                                                                 >  1:1201124/1‑151 (MQ=255)
                                                                gATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTcg                                                                                  <  1:1882815/150‑1 (MQ=255)
                                                                     gcaaTAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCGTTGCACCTGATGTTTTTCCATCGCACTGATCACCGCTTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTGCTGTGCTGTTcgcg                                                                                <  3:358861/144‑1 (MQ=255)
                                                                       gATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCAt                                                                           <  3:193508/150‑1 (MQ=255)
                                                                          aaGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGTCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTcgc                                                                       <  2:98944/151‑1 (MQ=255)
                                                                          aaGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTcgc                                                                       >  3:521151/1‑151 (MQ=255)
                                                                          aaGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTcg                                                                        <  4:1626397/150‑1 (MQ=255)
                                                                            gTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTcgcgc                                                                     >  1:403705/1‑151 (MQ=255)
                                                                               cATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCgg                                                                   <  4:1426075/150‑1 (MQ=255)
                                                                                aTGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGc                                                                  >  2:21352/1‑150 (MQ=255)
                                                                                  gCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAAt                                                               <  2:521171/151‑1 (MQ=255)
                                                                                   cACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATg                                                              >  2:149505/1‑151 (MQ=255)
                                                                                      cgccgGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGt                                                           >  2:119788/1‑151 (MQ=255)
                                                                                      cgccgGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGt                                                           >  1:431291/1‑151 (MQ=255)
                                                                                      cgccgGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGt                                                           <  2:214474/151‑1 (MQ=255)
                                                                                       gccgGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGtt                                                          <  1:1135290/151‑1 (MQ=255)
                                                                                          gggCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTgc                                                       <  2:496939/151‑1 (MQ=255)
                                                                                            gCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTgcgc                                                     <  1:696203/151‑1 (MQ=255)
                                                                                            gCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTgcgc                                                     <  1:653347/151‑1 (MQ=255)
                                                                                             cGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTgcgc                                                     >  1:338461/1‑150 (MQ=255)
                                                                                             cGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGAGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGAGCATTCGCGCGGCAATGCGTTTg                                                        >  4:763514/1‑147 (MQ=255)
                                                                                              gATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGcac                                                   >  4:674256/1‑151 (MQ=255)
                                                                                               aTGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGcaca                                                  <  2:338783/151‑1 (MQ=255)
                                                                                               aTGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGcaca                                                  <  3:199075/151‑1 (MQ=255)
                                                                                                 gggTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCacaaa                                                <  2:354106/151‑1 (MQ=255)
                                                                                                 gggTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCacaaa                                                <  1:561344/151‑1 (MQ=255)
                                                                                                 gggTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCacaa                                                 >  4:1578248/1‑150 (MQ=255)
                                                                                                  ggTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCacaaac                                               <  4:1434572/151‑1 (MQ=255)
                                                                                                   gTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCacaaaca                                              <  2:540379/151‑1 (MQ=255)
                                                                                                    tATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCacaaaca                                              >  4:874560/1‑150 (MQ=255)
                                                                                                     aTGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCacaaacaa                                             <  4:686872/150‑1 (MQ=255)
                                                                                                     aTGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAaacaac                                            <  1:740977/151‑1 (MQ=255)
                                                                                                       gCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACgg                                          <  4:1302650/151‑1 (MQ=255)
                                                                                                          cGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGc                                         >  4:1525195/1‑149 (MQ=255)
                                                                                                           gTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAgg                                      >  4:1290209/1‑151 (MQ=255)
                                                                                                            tGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAggg                                     >  3:289251/1‑151 (MQ=255)
                                                                                                              gATCAGCCATTGCACCTGATGTTTTTCCAACGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAggg                                     <  4:1052853/149‑1 (MQ=255)
                                                                                                               aTCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCg                                   >  2:168209/1‑150 (MQ=255)
                                                                                                               aTCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGa                                  <  1:719469/151‑1 (MQ=255)
                                                                                                                tCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCg                                   >  1:1171570/1‑149 (MQ=255)
                                                                                                                tCAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCg                                   >  1:1225459/1‑149 (MQ=255)
                                                                                                                 cAGCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAg                                 <  3:243354/150‑1 (MQ=255)
                                                                                                                   gCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGaa                              <  3:380495/151‑1 (MQ=255)
                                                                                                                   gCCATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGaa                              <  1:1889914/151‑1 (MQ=255)
                                                                                                                    ccATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGaa                              >  4:1351314/1‑150 (MQ=255)
                                                                                                                    ccATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAAt                             >  1:978193/1‑151 (MQ=255)
                                                                                                                     cATTGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATa                            <  1:686112/151‑1 (MQ=255)
                                                                                                                       ttGCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGc                          >  1:697370/1‑151 (MQ=255)
                                                                                                                         gCACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCg                         <  4:1071494/150‑1 (MQ=255)
                                                                                                                          cACCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTc                       <  4:616320/151‑1 (MQ=255)
                                                                                                                           aCCTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCt                      >  1:304907/1‑151 (MQ=255)
                                                                                                                            ccTGATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTg                     <  1:630226/151‑1 (MQ=255)
                                                                                                                               gATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCa                   <  4:691970/150‑1 (MQ=255)
                                                                                                                               gATGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCAg                  <  3:35909/151‑1 (MQ=255)
                                                                                                                                 tGTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCAGcc                >  3:66179/1‑151 (MQ=255)
                                                                                                                                  gTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCAGcc                >  1:758288/1‑150 (MQ=255)
                                                                                                                                  gTTTTTCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCAGCCa               <  1:706822/151‑1 (MQ=255)
                                                                                                                                    ttttCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCAGCCAgg             >  4:813056/1‑151 (MQ=255)
                                                                                                                                     tttCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCAGCCAggg            >  4:606683/1‑151 (MQ=255)
                                                                                                                                      ttCCATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCAGCCAggg            >  2:325798/1‑150 (MQ=255)
                                                                                                                                         cATCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTtg        >  4:1804034/1‑151 (MQ=255)
                                                                                                                                          aTCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTtgt       <  1:576206/151‑1 (MQ=255)
                                                                                                                                          aTCGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTtg        >  2:169892/1‑150 (MQ=255)
                                                                                                                                            cGCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTtgtg      <  1:588126/150‑1 (MQ=255)
                                                                                                                                            agCACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTgg     >  1:1083748/2‑151 (MQ=255)
                                                                                                                                              cACTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGAc   >  1:549163/1‑151 (MQ=255)
                                                                                                                                                cTGATCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGGTTGTGGAcc  >  3:153329/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACC  >  NC_000913/553255‑553548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑