BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	586,073	C→T	pseudogene (17/45 nt)	pauD →	tRNA‑OTHER

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	586,073	0	C	T	99.2%	510.2 / NA	131	pseudogene (17/45 nt)	pauD	tRNA‑OTHER
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (54/76); total (54/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

AAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACA  >  NC_000913/585927‑586219

aaaGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGc                                                                                                                                                  >  1:1089593/1‑149 (MQ=255)

  agagGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGTCGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGt                                                                                                                                              <  4:1108009/151‑1 (MQ=255)

  agagGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGt                                                                                                                                              <  4:1611864/151‑1 (MQ=255)

   gagGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGtt                                                                                                                                             <  1:1766801/151‑1 (MQ=255)

    agGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTc                                                                                                                                            <  1:1095445/151‑1 (MQ=255)

    agGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTc                                                                                                                                            <  1:553831/151‑1 (MQ=255)

    agGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTc                                                                                                                                            >  1:1439595/1‑151 (MQ=255)

     gggACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCg                                                                                                                                           <  1:342869/151‑1 (MQ=255)

       gACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGaa                                                                                                                                         <  1:932289/151‑1 (MQ=255)

           aaaCTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCt                                                                                                                                     <  1:1620884/151‑1 (MQ=255)

                gagaCACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCa                                                                                                                                  <  1:273096/149‑1 (MQ=255)

                gagaCACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAgg                                                                                                                                <  4:319975/151‑1 (MQ=255)

                gagaCACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAg                                                                                                                                 <  1:132773/150‑1 (MQ=255)

                 agaCACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAggg                                                                                                                               <  4:134514/151‑1 (MQ=255)

                  gaCACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGgc                                                                                                                              >  4:940624/1‑151 (MQ=255)

                     acaTAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGgcgcg                                                                                                                           <  1:1614502/151‑1 (MQ=255)

                          aGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCAtt                                                                                                                      <  1:535197/151‑1 (MQ=255)

                            gCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATttc                                                                                                                    <  1:382047/151‑1 (MQ=255)

                            gCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATttc                                                                                                                    <  1:253711/151‑1 (MQ=255)

                             ccTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATttct                                                                                                                   <  4:1864529/151‑1 (MQ=255)

                             ccTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATttct                                                                                                                   <  1:626033/151‑1 (MQ=255)

                              cTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATttctt                                                                                                                  >  4:530355/1‑151 (MQ=255)

                               tCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATttcttc                                                                                                                 >  1:24376/1‑151 (MQ=255)

                                cGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGTCGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATttctt                                                                                                                  <  4:54500/149‑1 (MQ=255)

                                cGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTcc                                                                                                                <  3:190662/151‑1 (MQ=255)

                                cGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTcc                                                                                                                >  4:419399/1‑151 (MQ=255)

                                cGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTcc                                                                                                                >  1:1203036/1‑151 (MQ=255)

                                      ggCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCAttt                                                                                                          <  4:1796541/151‑1 (MQ=255)

                                        cTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTAt                                                                                                        >  4:181641/1‑151 (MQ=255)

                                             aaGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCg                                                                                                   >  2:264764/1‑151 (MQ=255)

                                             aaGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCg                                                                                                   >  4:1691969/1‑151 (MQ=255)

                                              aGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGt                                                                                                  <  2:32876/151‑1 (MQ=255)

                                               ggCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTc                                                                                                 <  2:535751/151‑1 (MQ=255)

                                                    tACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCtt                                                                                               <  4:500063/148‑1 (MQ=255)

                                                    tACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTAt                                                                                             <  1:844294/150‑1 (MQ=255)

                                                    tACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATc                                                                                            >  1:1252786/1‑151 (MQ=255)

                                                     aCATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATcc                                                                                           <  4:713111/151‑1 (MQ=255)

                                                      cATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCg                                                                                          <  4:1922272/151‑1 (MQ=255)

                                                       aTGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCg                                                                                          <  4:1198884/150‑1 (MQ=255)

                                                       aTGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGt                                                                                         <  1:227663/151‑1 (MQ=255)

                                                         gTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGttt                                                                                       >  1:578262/1‑151 (MQ=255)

                                                              gAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCt                                                                                  <  4:1735983/151‑1 (MQ=255)

                                                               aGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCtt                                                                                 <  1:713223/151‑1 (MQ=255)

                                                                ggTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCttt                                                                                >  4:1417558/1‑151 (MQ=255)

                                                                 gTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTg                                                                               <  1:1686243/151‑1 (MQ=255)

                                                                  tAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGc                                                                              >  4:1010679/1‑151 (MQ=255)

                                                                   aGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGc                                                                              <  2:445221/150‑1 (MQ=255)

                                                                     tGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGATCTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGccc                                                                            <  2:240164/150‑1 (MQ=255)

                                                                     tGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTGCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGccc                                                                            <  4:711161/150‑1 (MQ=255)

                                                                      gggACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCtt                                                                          <  4:969368/151‑1 (MQ=255)

                                                                       ggACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTc                                                                         <  1:181589/151‑1 (MQ=255)

                                                                         aCGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTcac                                                                       <  3:308956/151‑1 (MQ=255)

                                                                          cGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGGCTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTcacc                                                                      <  1:492266/151‑1 (MQ=255)

                                                                          cGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTcacc                                                                      >  1:8475/1‑151 (MQ=255)

                                                                               gAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACAt                                                                  <  4:475255/150‑1 (MQ=255)

                                                                                aGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATc                                                                 >  1:746177/1‑150 (MQ=255)

                                                                                   gcAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACtt                                                             <  2:16911/151‑1 (MQ=255)

                                                                                    cAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACttt                                                            >  2:375671/1‑151 (MQ=255)

                                                                                     agagATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACtttt                                                           >  1:1138436/1‑151 (MQ=255)

                                                                                       agaTGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTg                                                         <  1:1876645/151‑1 (MQ=255)

                                                                                         aTGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTc                                                       <  4:356447/151‑1 (MQ=255)

                                                                                           ggCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCg                                                      <  1:1250561/150‑1 (MQ=255)

                                                                                            gcgcGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCt                                                    <  4:1748232/151‑1 (MQ=255)

                                                                                            gcgcGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCt                                                    <  1:333787/151‑1 (MQ=255)

                                                                                              gcgGTACGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGt                                                  >  1:497175/1‑151 (MQ=255)

                                                                                              gcgGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGt                                                  >  4:967078/1‑151 (MQ=255)

                                                                                               cgGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGtt                                                 <  4:1396394/151‑1 (MQ=255)

                                                                                                  tAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTgg                                              >  1:1152987/1‑151 (MQ=255)

                                                                                                   aaGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGc                                             >  1:250870/1‑151 (MQ=255)

                                                                                                        gttgACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGAt                                        <  4:234718/151‑1 (MQ=255)

                                                                                                           gACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAAt                                     >  1:1349672/1‑151 (MQ=255)

                                                                                                            aCTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAAtt                                    <  2:213319/151‑1 (MQ=255)

                                                                                                               tAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCa                                  >  1:185555/1‑150 (MQ=255)

                                                                                                                 aaaTGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACg                               >  1:1716488/1‑151 (MQ=255)

                                                                                                                   aTGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGgt                             >  1:1895564/1‑151 (MQ=255)

                                                                                                                     gTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGgtgt                           <  4:1357489/151‑1 (MQ=255)

                                                                                                                     gTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGgtgt                           <  3:295950/151‑1 (MQ=255)

                                                                                                                     gTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGgtg                            <  1:940220/150‑1 (MQ=255)

                                                                                                                      tCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTc                          >  4:1185037/1‑151 (MQ=255)

                                                                                                                       cGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTc                          >  1:650793/1‑150 (MQ=255)

                                                                                                                       cGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCa                         <  2:405969/151‑1 (MQ=255)

                                                                                                                       cGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCa                         <  4:1050321/151‑1 (MQ=255)

                                                                                                                          tCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAgg                       <  2:266498/150‑1 (MQ=255)

                                                                                                                            tAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCg                    >  4:115280/1‑151 (MQ=255)

                                                                                                                             aGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGcc                     <  4:890140/149‑1 (MQ=255)

                                                                                                                              ggAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGt                   >  4:156254/1‑150 (MQ=255)

                                                                                                                              ggAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTa                  <  3:376863/151‑1 (MQ=255)

                                                                                                                                aaCTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTaa                 >  4:848669/1‑150 (MQ=255)

                                                                                                                                aaCTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTaa                 >  1:1498214/1‑150 (MQ=255)

                                                                                                                                 aCTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTaaaa               <  1:444586/151‑1 (MQ=255)

                                                                                                                                   ttCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACg             >  4:661201/1‑151 (MQ=255)

                                                                                                                                    tCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAAc              <  2:454633/149‑1 (MQ=255)

                                                                                                                                       aaGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCtt         <  4:1337041/151‑1 (MQ=255)

                                                                                                                                       aaGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCtt         >  4:1629439/1‑151 (MQ=255)

                                                                                                                                        aGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCtt         >  4:829517/1‑150 (MQ=255)

                                                                                                                                           cGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTaaa      <  4:881226/150‑1 (MQ=255)

                                                                                                                                            gTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTaaaaa    <  1:1326604/151‑1 (MQ=255)

                                                                                                                                            gTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTaaaa     >  4:1546275/1‑150 (MQ=255)

                                                                                                                                              gggCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAAc   <  4:1402352/150‑1 (MQ=255)

                                                                                                                                              gggCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACa  <  1:1594557/151‑1 (MQ=255)

AAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACA  >  NC_000913/585927‑586219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`