Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 701,574 A→G A7A (GCT→GCC)  nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913701,5740AG99.3% 567.6 / NA 152A7A (GCT→GCC) nagCN‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (87/64);  minor base T (0/1);  total (87/65)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 152 total aligned reads displayed.

AAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGC  >  NC_000913/701427‑701721
                                                                                                                                                   |                                                                                                                                                   
aaGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCt                                                                                                                                                  >  4:360654/1‑151 (MQ=255)
 aGCTGACGCTTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCGCGGCAATCTGAATCCGCGAGATTGGCCGGTACTGGTAAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCtt                                                                                                                                                 <  1:640212/151‑1 (MQ=255)
 aGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAACTGTTTTACGAGATCAACATTACCTATCTGGGCtt                                                                                                                                                 >  1:261793/1‑151 (MQ=255)
     gACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTcc                                                                                                                                             >  1:1082210/1‑151 (MQ=255)
      aCGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCg                                                                                                                                            >  3:256180/1‑151 (MQ=255)
       cgcgTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCTGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGc                                                                                                                                           >  4:1010838/1‑151 (MQ=255)
        gcgTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGcc                                                                                                                                          >  4:1295513/1‑151 (MQ=255)
         cgTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCt                                                                                                                                         >  1:765351/1‑151 (MQ=255)
          gTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGc                                                                                                                                           <  1:719746/148‑1 (MQ=255)
                ttGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTc                                                                                                                                   <  4:479639/150‑1 (MQ=255)
                ttGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAACCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTc                                                                                                                                   <  4:65386/150‑1 (MQ=255)
                 tGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCAt                                                                                                                                 >  3:323072/1‑151 (MQ=255)
                  ggTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCAt                                                                                                                                 <  4:487004/150‑1 (MQ=255)
                    ttACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACt                                                                                                                              >  4:645344/1‑151 (MQ=255)
                      aCGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACtt                                                                                                                             <  4:492725/150‑1 (MQ=255)
                      aCGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACtt                                                                                                                             <  2:297806/150‑1 (MQ=255)
                       cGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTtc                                                                                                                           >  4:310644/1‑151 (MQ=255)
                        gCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTtc                                                                                                                           <  1:849856/150‑1 (MQ=255)
                           ggCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTtctc                                                                                                                         <  4:1548581/149‑1 (MQ=255)
                           ggCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCtt                                                                                                                       >  1:1582007/1‑151 (MQ=255)
                           ggCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAACCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTtctc                                                                                                                         <  2:453028/149‑1 (MQ=255)
                             cGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTAt                                                                                                                     >  4:1479554/1‑151 (MQ=255)
                              gggggCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTAt                                                                                                                     <  1:1843039/150‑1 (MQ=255)
                                gggCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGa                                                                                                                  <  4:1113503/151‑1 (MQ=255)
                                gggCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGa                                                                                                                  <  1:1618242/151‑1 (MQ=255)
                                 ggCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAg                                                                                                                 <  4:394123/151‑1 (MQ=255)
                                 ggCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAg                                                                                                                 <  4:1328314/151‑1 (MQ=255)
                                  gCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGt                                                                                                                <  4:343811/151‑1 (MQ=255)
                                    aaGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGtt                                                                                                               >  2:168628/1‑150 (MQ=255)
                                    aaGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTAATGAGtt                                                                                                               <  4:421269/150‑1 (MQ=255)
                                     aGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTAc                                                                                                             <  1:1198155/151‑1 (MQ=255)
                                     aGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTAc                                                                                                             >  3:315834/1‑151 (MQ=255)
                                      gCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACg                                                                                                            >  1:222379/1‑151 (MQ=255)
                                         ggCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGAcc                                                                                                         >  4:1669690/1‑151 (MQ=255)
                                         ggCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGAcc                                                                                                         <  1:935903/151‑1 (MQ=255)
                                          gCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCt                                                                                                        <  4:1687562/151‑1 (MQ=255)
                                            tttGCTCGGCAGTCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCg                                                                                                      >  4:865968/1‑151 (MQ=255)
                                             ttGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTc                                                                                                       <  2:253831/149‑1 (MQ=255)
                                             ttGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTc                                                                                                       <  1:197106/149‑1 (MQ=255)
                                              tGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGt                                                                                                     <  1:235556/150‑1 (MQ=255)
                                               gCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTa                                                                                                   <  3:168621/151‑1 (MQ=255)
                                                  cGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCg                                                                                                >  3:291866/1‑151 (MQ=255)
                                                    gCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGtt                                                                                              <  1:1683285/151‑1 (MQ=255)
                                                           gAATCCGCGAGATTGGCCCGTACGGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGa                                                                                         <  1:1271730/149‑1 (MQ=255)
                                                               ccGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAACCCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTc                                                                                    <  1:1164617/150‑1 (MQ=255)
                                                               ccGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCt                                                                                   >  1:711864/1‑151 (MQ=255)
                                                                 gcgAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCtt                                                                                  >  2:76784/1‑150 (MQ=255)
                                                                      aTTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGAt                                                                            >  4:822772/1‑151 (MQ=255)
                                                                        tGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGAttt                                                                          >  1:1734545/1‑151 (MQ=255)
                                                                           cccGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTa                                                                        <  1:1402574/150‑1 (MQ=255)
                                                                              gTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAAt                                                                    <  4:1749966/151‑1 (MQ=255)
                                                                                aCTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATc                                                                   <  1:1566791/150‑1 (MQ=255)
                                                                                aCTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATc                                                                   >  3:346800/1‑150 (MQ=255)
                                                                                aCTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCa                                                                  <  1:471352/151‑1 (MQ=255)
                                                                                 cTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATc                                                                   >  4:327109/1‑149 (MQ=255)
                                                                                 cTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCa                                                                  <  3:480615/150‑1 (MQ=255)
                                                                                  tGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAgg                                                                >  4:467232/1‑151 (MQ=255)
                                                                                  tGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAgg                                                                >  2:59648/1‑151 (MQ=255)
                                                                                     tCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGgtgt                                                             >  1:606483/1‑151 (MQ=255)
                                                                                     tCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGgtgt                                                             >  4:909321/1‑151 (MQ=255)
                                                                                       aaTCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGa                                                           <  1:151667/151‑1 (MQ=255)
                                                                                        aTCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGa                                                           >  4:80429/1‑150 (MQ=255)
                                                                                           aGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGc                                                       >  1:1299202/1‑151 (MQ=255)
                                                                                           aGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGc                                                       >  4:1636891/1‑151 (MQ=255)
                                                                                             gCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGc                                                       >  4:1430212/1‑149 (MQ=255)
                                                                                              cGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGt                                                    <  4:1209546/151‑1 (MQ=255)
                                                                                               gATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTc                                                   <  4:1831917/151‑1 (MQ=255)
                                                                                                aTAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTc                                                   >  2:274641/1‑150 (MQ=255)
                                                                                                 tAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTc                                                   >  4:1896197/1‑149 (MQ=255)
                                                                                                   aaCCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGt                                               <  3:159283/151‑1 (MQ=255)
                                                                                                    aCCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGtt                                              <  4:704125/151‑1 (MQ=255)
                                                                                                    aCCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGtt                                              <  1:653877/151‑1 (MQ=255)
                                                                                                        ccgcGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCt                                          >  1:451283/1‑151 (MQ=255)
                                                                                                         cgcgCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCt                                          >  4:182844/1‑150 (MQ=255)
                                                                                                         cgcgCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTa                                         <  1:931259/151‑1 (MQ=255)
                                                                                                          gcgcTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTAc                                        <  1:1471299/151‑1 (MQ=255)
                                                                                                           cgcTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACt                                       >  3:514733/1‑151 (MQ=255)
                                                                                                           cgcTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACt                                       >  3:10517/1‑151 (MQ=255)
                                                                                                             cTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACttt                                     >  4:421608/1‑151 (MQ=255)
                                                                                                               gTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTAc                                   >  1:1411102/1‑151 (MQ=255)
                                                                                                               gTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTAc                                   >  4:709002/1‑151 (MQ=255)
                                                                                                                 tAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTAcc                                  <  2:483822/150‑1 (MQ=255)
                                                                                                                  aaGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTg                                >  1:1049749/1‑151 (MQ=255)
                                                                                                                   aGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTgc                               >  2:355193/1‑151 (MQ=255)
                                                                                                                   aGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTgc                               >  4:1114016/1‑151 (MQ=255)
                                                                                                                       gTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTgcggc                            <  4:1368160/150‑1 (MQ=255)
                                                                                                                        ttttACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTgcggcg                           >  4:957681/1‑150 (MQ=255)
                                                                                                                           tACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAgtg                       >  4:568244/1‑151 (MQ=255)
                                                                                                                                gaTCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCg                  >  4:579895/1‑151 (MQ=255)
                                                                                                                                       aTTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGtt           >  4:1363249/1‑151 (MQ=255)
                                                                                                                                       aTTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGCCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGtt           >  4:1219454/1‑151 (MQ=255)
                                                                                                                                        ttACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGttt          >  4:1408181/1‑151 (MQ=255)
                                                                                                                                         tACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGttt          <  4:1506953/150‑1 (MQ=255)
                                                                                                                                         tACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGttt          >  4:1062088/1‑150 (MQ=255)
                                                                                                                                         tACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTtc         >  1:1460166/1‑151 (MQ=255)
                                                                                                                                             tATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCa      >  3:531668/1‑150 (MQ=255)
                                                                                                                                              aTCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAAc    >  4:1679685/1‑151 (MQ=255)
                                                                                                                                               tCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACg   >  4:1611896/1‑151 (MQ=255)
                                                                                                                                               tCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACg   >  3:449980/1‑151 (MQ=255)
                                                                                                                                                cTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGc  >  1:285759/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGC  >  NC_000913/701427‑701721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑