Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 96,645 T→C V215A (GTG→GCG)  mraY → phospho‑N‑acetylmuramoyl‑pentapeptide transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091396,6450TC99.4% 573.1 / NA 163V215A (GTG→GCG) mraYphospho‑N‑acetylmuramoyl‑pentapeptide transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (68/93);  minor base G (0/1);  total (69/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 166 total aligned reads displayed.

AAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCT  >  NC_000913/96498‑96791
                                                                                                                                                   |                                                                                                                                                  
aaGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTggcggc                                                                                                                                                 <  1:561064/151‑1 (MQ=255)
  gATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGgcggcg                                                                                                                                                >  1:1647723/1‑150 (MQ=255)
  gATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGt                                                                                                                                               <  4:1039918/151‑1 (MQ=255)
  gATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGt                                                                                                                                               >  4:284141/1‑151 (MQ=255)
    tgtgATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTTGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTg                                                                                                                                              >  4:1886333/1‑150 (MQ=255)
    tgtgATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTgg                                                                                                                                             >  1:1700940/1‑151 (MQ=255)
     gtgATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTggg                                                                                                                                            <  1:1096357/151‑1 (MQ=255)
       gATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGc                                                                                                                                           <  1:808707/150‑1 (MQ=255)
       gATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCg                                                                                                                                          <  1:372220/151‑1 (MQ=255)
        aTGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCg                                                                                                                                          <  4:1058632/150‑1 (MQ=255)
               aGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCaa                                                                                                                                  <  1:1533382/151‑1 (MQ=255)
               aGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCaa                                                                                                                                  <  1:276749/151‑1 (MQ=255)
                gCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAat                                                                                                                                 <  2:73577/151‑1 (MQ=255)
                   ggggCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAatat                                                                                                                               <  1:1676249/150‑1 (MQ=255)
                   ggggCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATg                                                                                                                              <  4:427566/151‑1 (MQ=255)
                    gggCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAatat                                                                                                                               <  1:890460/149‑1 (MQ=255)
                    gggCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGa                                                                                                                             <  4:1703011/151‑1 (MQ=255)
                    gggCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGa                                                                                                                             <  1:670778/151‑1 (MQ=255)
                     ggCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGa                                                                                                                             >  2:427565/1‑150 (MQ=255)
                      gCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAAc                                                                                                                           <  4:1769587/151‑1 (MQ=255)
                      gCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAAc                                                                                                                           <  4:425894/151‑1 (MQ=255)
                          ttCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTg                                                                                                                       >  4:1314538/1‑151 (MQ=255)
                              aCATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCa                                                                                                                    <  2:16298/150‑1 (MQ=255)
                              aCATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCa                                                                                                                    <  4:157785/150‑1 (MQ=255)
                              aCATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAg                                                                                                                   <  1:117693/151‑1 (MQ=255)
                                aTTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCt                                                                                                                 >  1:226159/1‑151 (MQ=255)
                                  tCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTa                                                                                                                <  2:211158/150‑1 (MQ=255)
                                  tCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTa                                                                                                                <  4:1485149/150‑1 (MQ=255)
                                    tgctgGTTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACtt                                                                                                             >  2:29625/1‑151 (MQ=255)
                                     gctgGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTg                                                                                                            <  1:557817/151‑1 (MQ=255)
                                     gctgGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTg                                                                                                            >  1:707182/1‑151 (MQ=255)
                                         gCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGGGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCata                                                                                                        <  1:268271/151‑1 (MQ=255)
                                         gCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCata                                                                                                        <  4:1820535/151‑1 (MQ=255)
                                         gCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCata                                                                                                        <  1:821415/151‑1 (MQ=255)
                                           ttACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCatat                                                                                                       >  4:811884/1‑150 (MQ=255)
                                              cTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCg                                                                                                   >  4:1657519/1‑151 (MQ=255)
                                               ttCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATAcc                                                                                                    >  3:108395/1‑149 (MQ=255)
                                                 cGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTAt                                                                                                >  2:112019/1‑151 (MQ=255)
                                                  gTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATc                                                                                               >  2:267715/1‑151 (MQ=255)
                                                    cATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCt                                                                                              <  1:1265000/150‑1 (MQ=255)
                                                    cATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTg                                                                                             <  2:493692/151‑1 (MQ=255)
                                                       tgtgGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCg                                                                                           <  4:17224/150‑1 (MQ=255)
                                                       tgtgGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCg                                                                                           >  1:1778400/1‑150 (MQ=255)
                                                        gtgGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGa                                                                                          <  1:1768703/150‑1 (MQ=255)
                                                          gggTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGTCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACGTTGCCAGCTACTTGCATATACCGTAACTGCGac                                                                                         <  3:70099/149‑1 (MQ=255)
                                                          gggTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGaca                                                                                        <  3:339505/150‑1 (MQ=255)
                                                            gTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGc                                                                                     <  1:957090/151‑1 (MQ=255)
                                                              aCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGcc                                                                                    <  1:1034412/150‑1 (MQ=255)
                                                                tGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCggg                                                                                 >  4:1082594/1‑151 (MQ=255)
                                                                 ggCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCgggg                                                                                <  3:427551/151‑1 (MQ=255)
                                                                 ggCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCgggg                                                                                <  4:850951/151‑1 (MQ=255)
                                                                    aaCGCGGTAAGCCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATGCCGTATCTGCGACACGCCGGGGaa                                                                              <  1:1444761/150‑1 (MQ=255)
                                                                     aCGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACt                                                                            <  4:116548/151‑1 (MQ=255)
                                                                       gcgGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGAATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTgg                                                                          >  1:1562732/1‑151 (MQ=255)
                                                                        cgGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTgg                                                                          <  1:1505381/150‑1 (MQ=255)
                                                                        cgGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGt                                                                         <  4:280756/151‑1 (MQ=255)
                                                                          gTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGATTTGCGCTGGCGGCGTGGGAGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGtt                                                                        >  4:1172227/1‑150 (MQ=255)
                                                                           tAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTAt                                                                      >  4:1172754/1‑151 (MQ=255)
                                                                               ccTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTc                                                                  <  4:1478292/151‑1 (MQ=255)
                                                                                cTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCt                                                                 >  3:16266/1‑151 (MQ=255)
                                                                                cTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCt                                                                 >  4:348514/1‑151 (MQ=255)
                                                                                     cGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTAcc                                                            >  4:1716220/1‑151 (MQ=255)
                                                                                     cGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTAcc                                                            >  4:653111/1‑151 (MQ=255)
                                                                                     cGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTAcc                                                            <  1:1503436/151‑1 (MQ=255)
                                                                                         ggTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACcgcg                                                         <  4:1240309/150‑1 (MQ=255)
                                                                                         ggTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGa                                                        <  2:61850/151‑1 (MQ=255)
                                                                                             tcGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGt                                                    >  1:1148685/1‑151 (MQ=255)
                                                                                              cGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAg                                                     <  1:1923840/149‑1 (MQ=255)
                                                                                              cGACGGCCTGGCAATTATGCCGACCGTAATTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGt                                                    <  1:241997/150‑1 (MQ=255)
                                                                                               gACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCg                                                  <  2:454682/151‑1 (MQ=255)
                                                                                               gACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCg                                                  <  1:1180989/151‑1 (MQ=255)
                                                                                                 cGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCggg                                                >  1:1030950/1‑151 (MQ=255)
                                                                                                  ggCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCgggg                                               >  2:91233/1‑151 (MQ=255)
                                                                                                   gCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGc                                              >  2:426371/1‑151 (MQ=255)
                                                                                                    ccTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCa                                             <  3:23479/151‑1 (MQ=255)
                                                                                                        gCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGAc                                         <  1:1391701/151‑1 (MQ=255)
                                                                                                         cAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACt                                        >  1:1526356/1‑151 (MQ=255)
                                                                                                          aaTTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTg                                       >  3:380430/1‑151 (MQ=255)
                                                                                                              aTGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCt                                   >  2:20549/1‑151 (MQ=255)
                                                                                                              aTGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCt                                   <  4:1668091/151‑1 (MQ=255)
                                                                                                                gCCGACCGTTTTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGCCAAACGGCAATATGAACTTTGCAAGCTCCTTGCATATGCCGCATCTGCGACACGCCGGGGAACTGGTTAGTGTGGGTACCGCGATAGTCGGGGCGCGACTGGGCtt                                  <  1:1286829/150‑1 (MQ=255)
                                                                                                                  cGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTcc                                >  1:1827146/1‑150 (MQ=255)
                                                                                                                  cGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCt                               <  3:28152/151‑1 (MQ=255)
                                                                                                                   gACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCtg                              <  1:1071858/151‑1 (MQ=255)
                                                                                                                   gACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCtg                              >  1:611847/1‑151 (MQ=255)
                                                                                                                      cGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTgg                           >  1:869223/1‑151 (MQ=255)
                                                                                                                       gTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGt                          >  4:1801010/1‑151 (MQ=255)
                                                                                                                           ttGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTa                       <  1:1238440/150‑1 (MQ=255)
                                                                                                                              tCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAacac                   >  3:84292/1‑151 (MQ=255)
                                                                                                                               cGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACAcc                  >  4:1122004/1‑151 (MQ=255)
                                                                                                                               cGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACAcc                  >  4:1279494/1‑151 (MQ=255)
                                                                                                                                  cGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTAt               <  1:1869105/151‑1 (MQ=255)
                                                                                                                                      ggtTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCgg           <  3:20549/151‑1 (MQ=255)
                                                                                                                                       gtTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGgc          <  1:1293588/151‑1 (MQ=255)
                                                                                                                                        ttttGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGttt                        <  4:921404/136‑1 (MQ=255)
                                                                                                                                           tGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCa       <  4:1051771/150‑1 (MQ=255)
                                                                                                                                              gcTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTc   >  1:1365742/1‑151 (MQ=255)
                                                                                                                                              gcTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTc   >  3:240815/1‑151 (MQ=255)
                                                                                                                                              gcTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTc   <  3:88571/151‑1 (MQ=255)
                                                                                                                                               cTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCt  >  4:1396178/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCT  >  NC_000913/96498‑96791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: