Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 781,084 T→C noncoding (17/76 nt) valZ → tRNA‑Val

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913781,0840TC98.7% 487.8 / ‑1.7 149noncoding (17/76 nt)valZtRNA‑Val
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (70/76);  total (72/77)
Fisher's exact test for biased strand distribution p-value = 2.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.46e-01

Only 100 of 149 total aligned reads displayed.

AGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAAC  >  NC_000913/780936‑781230
                                                                                                                                                    |                                                                                                                                                   
aGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCgg                                                                                                                                                   <  2:474321/151‑1 (MQ=255)
aGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGGGATTAGCTCAGCCgg                                                                                                                                                   >  1:1860634/1‑151 (MQ=255)
aGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATACAGCGCAGTATCGGGTGATTAGCTTAGCCgg                                                                                                                                                   >  1:884774/1‑151 (MQ=255)
aGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTACGGGGGACGAAGTTTGGAGTATCAAGCGCAGTATCGGGTGATTAGCTCAGCCgg                                                                                                                                                   >  1:169784/1‑151 (MQ=21)
 gATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCggg                                                                                                                                                  >  1:1635837/1‑151 (MQ=255)
 gATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCgg                                                                                                                                                   <  1:1045655/150‑1 (MQ=255)
  aTGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGga                                                                                                                                                 <  1:415135/151‑1 (MQ=255)
  aTGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGga                                                                                                                                                 <  4:1793155/151‑1 (MQ=255)
      tAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGagag                                                                                                                                              <  1:68451/150‑1 (MQ=255)
       aaGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGc                                                                                                                                             <  4:840508/150‑1 (MQ=255)
           gtgtAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTc                                                                                                                                        <  3:70105/151‑1 (MQ=255)
            tgtAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTcc                                                                                                                                       >  1:26175/1‑151 (MQ=255)
            tgtAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTcc                                                                                                                                       >  4:903345/1‑151 (MQ=255)
            tgtAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGGGATTAGCTCAGCCGGGAGAGCACCTcc                                                                                                                                       >  1:212640/1‑151 (MQ=255)
             gtAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTccc                                                                                                                                      >  1:1789589/1‑151 (MQ=255)
              tAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCt                                                                                                                                     >  2:303430/1‑151 (MQ=255)
                ggATAACGTTGCGTCAGCAACGTCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTa                                                                                                                                   <  4:72276/151‑1 (MQ=255)
                   tAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACa                                                                                                                                 >  2:91468/1‑150 (MQ=255)
                       gTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCGTCCTGCCTGACCCCCCACTAATGACGGTGGGTTCGGTGGAAGTAGGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAagga                                                                                                                             <  1:794214/150‑1 (MQ=37)
                         tGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAggg                                                                                                                          >  1:1398435/1‑151 (MQ=255)
                          gCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGAGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCGCAGCCGGGAGAGCACCTCCCTTACAAGGAgggg                                                                                                                         >  3:90597/1‑151 (MQ=255)
                           cGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAggggg                                                                                                                        <  1:1013409/151‑1 (MQ=255)
                              cAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTc                                                                                                                      <  4:1219218/150‑1 (MQ=255)
                               aGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTcgg                                                                                                                    <  1:1591121/151‑1 (MQ=255)
                               aGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGGTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTcgg                                                                                                                    >  1:1038948/1‑151 (MQ=255)
                                  aaCGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTcggcgg                                                                                                                 >  4:1681782/1‑151 (MQ=255)
                                  aaCGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCAACTCCCTTACAAGGAGGGGGTcggcgg                                                                                                                 >  1:1373592/1‑151 (MQ=255)
                                     ggCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCTCGACCCTCCACTAATGCCGGTGGGTTCGGTGGAAGTTGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGtt                                                                                                               <  1:819442/150‑1 (MQ=25)
                                          gTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATcc                                                                                                         >  1:827597/1‑151 (MQ=255)
                                          gTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATcc                                                                                                         >  1:1823162/1‑151 (MQ=255)
                                           tAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGAAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACACGGAGGGGGTCGGCGGTTCGATccc                                                                                                        >  4:1368173/1‑151 (MQ=255)
                                            aGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATccc                                                                                                        >  4:826923/1‑150 (MQ=255)
                                            aGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCg                                                                                                       >  4:184961/1‑151 (MQ=255)
                                              ggCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGtc                                                                                                     >  2:72017/1‑151 (MQ=255)
                                                   gcgaagcgaGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGtcatca                                                                                                 <  2:306842/150‑1 (MQ=255)
                                                      aagcgaGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcaccca                                                                                             <  2:465785/151‑1 (MQ=255)
                                                         cgaGTCGTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcacccac                                                                                            <  4:488664/149‑1 (MQ=255)
                                                              cgtCCTGCTCGACCCTCCACTAATGCCGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCg                                                                                      <  4:609759/148‑1 (MQ=255)
                                                                 ccTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTc                                                                                  <  1:1740346/151‑1 (MQ=255)
                                                                 ccTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTc                                                                                  <  4:1279034/151‑1 (MQ=255)
                                                                 ccTGCACGACCCACCACTAATGACGGTGGGTTCGGGGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGATTGATTCCGTCCTcacccacccccccgg                                                                                    >  1:1883504/1‑142 (MQ=18)
                                                                    gCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCg                                                                                 <  4:1580484/149‑1 (MQ=255)
                                                                     cACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTa                                                                              <  4:1227163/151‑1 (MQ=255)
                                                                     cACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTa                                                                              <  2:344505/151‑1 (MQ=255)
                                                                     cACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCCGGTCGtt                                                                               >  1:1719284/1‑150 (MQ=255)
                                                                      aCGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCTCCCCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAg                                                                             <  1:122205/151‑1 (MQ=255)
                                                                      aCGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTa                                                                              <  4:1815339/150‑1 (MQ=255)
                                                                       cgccccACTGCTAATGACGCTTGGTCCGTTGGGAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTa                                                                              <  4:358934/146‑1 (MQ=17)
                                                                        gccagtccacTAATGACGGTGGGTTCGGTGGAAGTAGGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCt                                                                           <  1:1817493/145‑1 (MQ=255)
                                                                        gACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGc                                                                            >  4:312706/1‑150 (MQ=255)
                                                                         aCCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTc                                                                          <  4:1145838/151‑1 (MQ=255)
                                                                               cacTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTgg                                                                    <  4:1916007/151‑1 (MQ=255)
                                                                                   aaTGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTa                                                                  <  1:989698/149‑1 (MQ=255)
                                                                                    aTGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTag                                                                 <  2:249558/149‑1 (MQ=255)
                                                                                    aTGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTag                                                                 >  4:310511/1‑149 (MQ=255)
                                                                                      gACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGc                                                              <  4:173811/150‑1 (MQ=255)
                                                                                       aCGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACGAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAg                                                            <  1:847861/151‑1 (MQ=255)
                                                                                         ggTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGtt                                                          <  2:124077/151‑1 (MQ=255)
                                                                                         ggTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGtt                                                          <  4:1003877/151‑1 (MQ=255)
                                                                                           tGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGtt                                                          >  2:242854/1‑149 (MQ=255)
                                                                                             ggctcgaacgAAGTTGGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAg                                                            <  3:325755/136‑1 (MQ=11)
                                                                                              gTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑tt                                                    >  1:307642/1‑151 (MQ=255)
                                                                                               ttCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCCCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑ttt                                                   >  3:467322/1‑151 (MQ=255)
                                                                                                  ggTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTaa                                                <  4:711157/151‑1 (MQ=255)
                                                                                                  ggTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTa                                                 >  3:248592/1‑150 (MQ=255)
                                                                                                  ggTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTa                                                 >  4:1169806/1‑150 (MQ=255)
                                                                                                    tGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATc                                              >  2:409067/1‑151 (MQ=255)
                                                                                                     ggAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCCGTTGGTAGAGCAGTTGAC‑TTTTAATc                                              >  1:747505/1‑150 (MQ=255)
                                                                                                     ggAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATc                                              <  1:111663/150‑1 (MQ=255)
                                                                                                     ggAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATc                                              >  1:1727198/1‑150 (MQ=255)
                                                                                                         gtagtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAAt                                           <  4:793269/149‑1 (MQ=255)
                                                                                                         gtagtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTg                                         <  4:1342210/151‑1 (MQ=255)
                                                                                                         gtaggTTGTAGTACCCAGCGGAGTATCGGGTGATTAGCTCAGCCGGGAGAGCCCCCCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCCCCCACCACCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTg                                         <  3:358644/151‑1 (MQ=11)
                                                                                                           agtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTgg                                        <  1:246080/150‑1 (MQ=38)
                                                                                                             tttGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTggac                                      >  3:177662/1‑148 (MQ=37)
                                                                                                             tttGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGt                                       >  4:983429/1‑149 (MQ=37)
                                                                                                                gtagtcTCCAGCGCAGTATCGGGTGATTAGCTCAGCCTGGAGAGCCCCTCCCTGACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGt                                       <  1:650074/146‑1 (MQ=255)
                                                                                                                  agtaTCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGTCGCAGGt                                <  2:104232/151‑1 (MQ=25)
                                                                                                                     aTCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGTCGCAGGTTc                              <  4:444172/150‑1 (MQ=25)
                                                                                                                      tCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGTCGCAGGTTCg                             >  4:12657/1‑150 (MQ=25)
                                                                                                                       ccAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGTCGCAGGTTCGaa                           <  4:537185/151‑1 (MQ=25)
                                                                                                                        cAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGTCGCCGGTTCGaa                           >  4:772754/1‑150 (MQ=25)
                                                                                                                        cAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGTCGCAGGTTCGAAt                          >  2:220689/1‑151 (MQ=25)
                                                                                                                         aGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGTCGCAGGTTCGAATc                         <  4:1711456/151‑1 (MQ=25)
                                                                                                                            gcAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGTCGCAGGTTCGAATCCt                       >  3:6054/1‑150 (MQ=25)
                                                                                                                              aGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCCTCACCCACCACTCGGGTCGTTAGCTCAGGTGGTAGAGCAGTTGAC‑TTTTCATCAATTGGGCGCAGGTTCGAATCCTGc                     >  3:397406/1‑150 (MQ=255)
                                                                                                                               gTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGTCGCAGGTTCGAATCCTGCAc                   <  1:1681117/151‑1 (MQ=18)
                                                                                                                                 aTCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGCGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCGGTTGGC‑TTTTAATCACTTGGTCGCCGGGTCGAATCCTGCACg                  >  3:47320/1‑150 (MQ=11)
                                                                                                                                 aTCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCCTCGCCCACCTCTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACg                  <  1:1153088/150‑1 (MQ=255)
                                                                                                                                 aTCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACg                  <  4:362715/150‑1 (MQ=18)
                                                                                                                                      gTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACcca             <  1:1508508/150‑1 (MQ=18)
                                                                                                                                       tGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCCGGGCCTTTGCCCCAATGGGAAGGGC‑GTTGGCTTTTTAATCAATGGGTCCCGGGTCCGAATcttcacggccc               >  4:1509106/1‑137 (MQ=2)
                                                                                                                                        gATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACccacca          <  1:1659627/151‑1 (MQ=18)
                                                                                                                                          ttAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGt        >  4:1657828/1‑151 (MQ=18)
                                                                                                                                          ttAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCAACAGt        >  1:281754/1‑151 (MQ=18)
                                                                                                                                           tAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCCGtt       >  2:84215/1‑151 (MQ=18)
                                                                                                                                            aGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGttt      >  1:326323/1‑151 (MQ=18)
                                                                                                                                             gCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGtttt     <  1:497034/151‑1 (MQ=18)
                                                                                                                                                 aGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTaa   >  2:505446/1‑149 (MQ=18)
                                                                                                                                                 aGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGGCGTTAGCTCAGTTGGTAGAGCAGGTGAC‑TTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTTaaa  >  1:1264891/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC‑TTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAAC  >  NC_000913/780936‑781230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑