| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 126,862 | C→T | P390S (CCG→TCG) | aceF → | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 126,862 | 0 | C | T | 91.4% | 419.3 / NA | 142 | P390S (CCG→TCG) | aceF | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Reads supporting (aligned to +/- strand): ref base C (2/0); major base T (57/71); minor base G (12/0); total (71/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.21e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 142 total aligned reads displayed.
GTTAACCTTGCGAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAA > NC_000913/126718‑127008 | gtTAACCTTGCGAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAgg > 1:1181277/1‑151 (MQ=255)gtTAACCTTGCGAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAg < 4:1825589/150‑1 (MQ=255) tAACCTTGCGAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGt < 4:705340/150‑1 (MQ=255) tAACCTTGCGAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGt < 1:909408/150‑1 (MQ=255) aCCTTGCGAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGa < 1:812923/151‑1 (MQ=255) ccTTGCGAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCCTGCTTCCGTGGTCGGAGGTGGa > 3:210126/1‑150 (MQ=255) tGCGAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACtt > 2:324126/1‑150 (MQ=255) tGCGAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACtt > 2:218308/1‑150 (MQ=255) gAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGc < 4:813717/151‑1 (MQ=255) gAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGc < 1:1184527/151‑1 (MQ=255) gAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCCGGCATGCTGCCGTGGTCGAAAGGGGACTTCa > 4:34649/1‑149 (MQ=255) aaGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGGCGAAGGGGGACTTc > 1:475107/1‑146 (MQ=255) gCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGGAGGTGGACTTCAGCAAGTTTgggg > 4:1420242/1‑148 (MQ=255) gCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCCGGCATGCTGCCGGGGGCGGAGGGGGACTTCAGGAAGGTTgggg > 1:571070/1‑148 (MQ=255) cACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGa < 1:1075357/150‑1 (MQ=255) aCTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGaa > 4:78585/1‑150 (MQ=255) ggCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAAt < 1:971817/149‑1 (MQ=255) ggCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATc < 4:1248918/150‑1 (MQ=255) ggCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATc < 4:1209733/150‑1 (MQ=255) ggCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCg < 4:1582424/151‑1 (MQ=255) ggCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCg < 3:107793/151‑1 (MQ=255) cGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGGGGACTTCAGCAAGTTTGGTGAAATCga > 3:427085/1‑149 (MQ=255) cGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGGGGACTTCAGCAAGTTTGGTGAAATCga > 4:1368245/1‑149 (MQ=255) gTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCgaaga < 4:48856/151‑1 (MQ=255) gTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGGGGACTTCAGCAAGTTTGGTGAAATCg > 2:77729/1‑147 (MQ=255) gTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGGGGACTTCAGCAAGTTTGGGGAAATCg > 1:1184282/1‑147 (MQ=255) tAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCgaaga < 1:1525942/150‑1 (MQ=255) tAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGGATCCCTGGCATGCTGCCGGGGGCGGAGGTGGGCTTCAGCAAGTTTGGTGAAATCgaaga > 1:1838276/1‑150 (MQ=255) aaaGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCgaagaa < 4:20375/150‑1 (MQ=255) aaGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGaagaag < 1:225797/150‑1 (MQ=255) ggTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTgg > 4:1034938/1‑151 (MQ=255) gTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGa < 1:351471/151‑1 (MQ=255) gTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGGGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTgg > 4:18858/1‑150 (MQ=255) gTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATTCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACt < 4:1607850/151‑1 (MQ=255) gTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACt < 4:1831267/151‑1 (MQ=255) aTCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTgg < 2:373611/151‑1 (MQ=255) aTCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTg < 4:1838996/150‑1 (MQ=255) cTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGc < 4:565468/150‑1 (MQ=255) tGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGGGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGGTTGGGGAAATCGAAGAAGTGGAAATgg > 4:267198/1‑147 (MQ=255) gggcgAAGACGGTCAGGCTTACGTGAATGAAGCTATCAAACGCGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGGATCCCTGGCATGCTGCCGTTTTAGTAGGTGGACTTCCGCGAACTTAGCGAAATTGAAGAAGTGGAACTGGGCCg > 1:280586/3‑150 (MQ=255) gcgcGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGTcc < 1:207941/149‑1 (MQ=255) gcgcGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGc < 1:1172070/151‑1 (MQ=255) gcgcGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGGATCCCTGGCATGCTGGCGGGGTCGAAGGAGGGCTTCAGCAAGTTTGGTGGAATCGAAGAAGTGGAACTGGGCCGc > 4:1561564/1‑151 (MQ=255) gAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATcc > 4:1452452/1‑151 (MQ=255) gACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGGTGCCGTGGGCGAAGGGGGACTTCAGCAAGGTTGGGGAAATCGAAGAAGTGGGACTGGGGCGCATcc > 1:331506/1‑148 (MQ=255) gACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATcc > 3:297487/1‑148 (MQ=255) gTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCCGGCATGCTGCCGTGGTCGCAGGTGGAATTCAGCGAGTTTGGTGGAATCGAAGAAGGGGAACTGGGGCGCCTCCAg > 1:299583/1‑147 (MQ=255) ttCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGGACTGGGCCGCATCCAGaaaa > 1:507841/1‑150 (MQ=255) tCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGGATCCCTGGGATGCTGCCGGGGTCGAAGGGGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGaaaa > 1:440302/1‑149 (MQ=255) cAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGGGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAAtc > 4:783093/1‑150 (MQ=255) ggCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCCTGGTGGCGTGGTCGAAGGGGGGCTTCAGCAAGTTTGGGGGAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATctct > 1:265418/1‑151 (MQ=255) gCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGGGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTg > 3:488776/1‑151 (MQ=255) tACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGt < 1:839526/150‑1 (MQ=255) cGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGGGGGATCCCTGGGATGCTGCCGTGGTCGAAGGGGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCg > 1:942214/1‑151 (MQ=255) tGACAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGCTGTATCCCTGGCATTTTTCCGCGGCGCAAGGTGGGCCTGAACTAGCTTGGAGGAATTGAAGGACTGGTACTCGGCCGGATCCCGCAAATCCCTg > 4:1263241/1‑144 (MQ=255) gAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGGATCCCCGGCCTGCTGCCGTGGGCGAAGGTGGACTTCAGCAAGTTTGGGGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGaa > 1:1904220/1‑150 (MQ=255) aagaagCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCt < 1:1133668/151‑1 (MQ=255) aagaagCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCt < 1:1844878/151‑1 (MQ=255) agaagCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGGATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAAATGGGCCGCATCCAGAAAATCTCTGGTGCGAAcc > 1:1639812/1‑149 (MQ=255) aTCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTTGCATGCTGCCGTGGTCGCAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACCGGGCCGCATCCCGAAAATCTCTGGTGCGGACCTGGGcc > 1:1827376/1‑148 (MQ=255) aTCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGt < 4:1829361/150‑1 (MQ=255) cAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTaa < 4:149290/150‑1 (MQ=255) cAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTaa < 3:162975/150‑1 (MQ=255) aaaCGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGGGGTCGAAGGGGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACt > 1:192214/1‑151 (MQ=255) cGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGGCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGGAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGGAACTggg > 2:451924/1‑151 (MQ=255) gTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATTCCTGGCCTGCTGGCGTGGTCGAAGGTGGACCTCAGCAAGTTTGGTGGAATCGGAGAAGTGGGACTGGGCCGCATCCAGAAAATCCCTGGGGCGAACCTGAACCGCAACTggg > 4:770159/1‑150 (MQ=255) gTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGt < 4:1226280/151‑1 (MQ=255) tGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGGGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTggg > 3:280342/1‑149 (MQ=255) tGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGGCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTggg > 4:668980/1‑149 (MQ=255) gCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGGCGAAGGTGGACTTCAGCAAGGTTGGGGAAATCGAAGAAGTGGGAACGGGCCGCATCCCGAAAATCTCTGGTGCGAACCTGAGCCGTAACTggg > 1:1158361/1‑148 (MQ=255) cAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAAt < 1:1657565/151‑1 (MQ=255) aGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAAt > 3:89264/1‑150 (MQ=255) aGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATg < 1:2558/151‑1 (MQ=255) agcagcTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGGGGACTTCAGCAAGTTTGGGGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGGAAt > 4:1610608/1‑147 (MQ=255) cagcTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATccc < 2:540648/151‑1 (MQ=255) agcTCCGGCAGCGACTGGCGGTGGGATCCCTGGCATGCTGCCGTGGTCGAAGGGGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATccc > 2:275142/1‑150 (MQ=255) gcTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGc < 1:1368016/151‑1 (MQ=255) cTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGGGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAAACTGAGCCGTAACTGGGTAATGATCCCGc > 3:440519/1‑150 (MQ=255) tCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGGGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCAt > 2:431147/1‑151 (MQ=255) ccGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCAt < 1:78487/150‑1 (MQ=255) ccGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATg > 4:659690/1‑151 (MQ=255) cGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATg > 4:1219822/1‑150 (MQ=255) ggCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGtt < 4:265497/151‑1 (MQ=255) gaaGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGGGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAATGGAACTGGGCCGCATCCAGAAAATCTCCGGTGCGAACCTGAGCCGTAACTGGGGAATGATTCCGCATGtt > 3:150327/3‑150 (MQ=255) cAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTAc < 4:599460/151‑1 (MQ=255) gCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACt < 1:769214/150‑1 (MQ=255) tGGCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTc > 1:1678873/1‑151 (MQ=255) ggCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTc < 1:1609975/150‑1 (MQ=255) ggCGGTGGTATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTc < 4:1731191/150‑1 (MQ=255) ggtggtATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGAc < 4:1099326/150‑1 (MQ=255) gtggtATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGAc > 3:375908/1‑149 (MQ=255) gtATCCCTGGCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAAc < 4:157910/151‑1 (MQ=255) cccTGGCATGCTGCCGTGGTCGTAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGa < 2:192037/150‑1 (MQ=255) ggCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGatat < 1:1490133/149‑1 (MQ=255) ggCATGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATc < 1:443318/150‑1 (MQ=255) aTGCTGCCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCg > 4:1760571/1‑151 (MQ=255) gctgcCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGa < 1:417306/150‑1 (MQ=255) ctgcCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGt > 1:1360742/1‑151 (MQ=255) gcCGTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGt < 1:1691698/149‑1 (MQ=255) gTGGTCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGa > 1:1044826/1‑150 (MQ=255) | GTTAACCTTGCGAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAA > NC_000913/126718‑127008 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |