BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,154,782	A→G	E224G (GAA→GGA)	yceG →	septation protein, ampicillin sensitivity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,154,782	0	A	G	100.0%	571.2 / NA	152	E224G (GAA→GGA)	yceG	septation protein, ampicillin sensitivity
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (84/68); total (84/68)

Only 100 of 152 total aligned reads displayed.

TCGCGTTACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACA  >  NC_000913/1154635‑1154928

tCGCGTTACTCAAGCGAGCGCACAAGCAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGAcg                                                                                                                                                 >  4:819385/1‑151 (MQ=255)

 cgcgTTACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGAcgc                                                                                                                                                >  4:1368759/1‑151 (MQ=255)

 cgcgTTACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGAcgc                                                                                                                                                >  3:315304/1‑151 (MQ=255)

     ttACTCAAGCGGGCGAAAAAGAAAATGGTGAAAGCGGTCGATGGCGCCTGGGAAGGGCGTGCGGACGGTCTGCTTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGa                                                                                                                                              <  4:848070/149‑1 (MQ=255)

      tACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATa                                                                                                                                            >  1:1728641/1‑150 (MQ=255)

      tACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATa                                                                                                                                            >  1:1249723/1‑150 (MQ=255)

        cTCAAGCGGGCGCACAAAAAAAGGGTGAAAGCGGTCGATAGCGCCGGGGAAGGGCGTGCGGACGTTCTGCCTTAAAAAGATAAAACCCAGTTGGTGACGATGGAATAAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATa                                                                                                                                            <  1:1106761/148‑1 (MQ=255)

        cTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGTTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAg                                                                                                                                          <  4:521704/150‑1 (MQ=255)

           aaGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTg                                                                                                                                      >  3:124250/1‑151 (MQ=255)

              tgagcgCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCt                                                                                                                                   >  3:213731/2‑151 (MQ=255)

                 gggaacaAGAAAAGGGTAAAAGCGGTCGATAGCGCGGGGAAAGGGCGTGCGGAGGTTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGAATAAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCa                                                                                                                                 <  4:1569912/146‑1 (MQ=255)

                 gcgcACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTc                                                                                                                                  >  1:1227737/1‑149 (MQ=255)

                  cgcACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAg                                                                                                                                >  1:1221556/1‑150 (MQ=255)

                  cgcACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTGGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAg                                                                                                                                <  2:45/150‑1 (MQ=255)

                   gcACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGt                                                                                                                               >  4:735167/1‑150 (MQ=255)

                    cacaAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTAt                                                                                                                             >  1:1279562/1‑151 (MQ=255)

                    cacaAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTAt                                                                                                                             >  3:155121/1‑151 (MQ=255)

                     acaAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTAtt                                                                                                                            >  1:1440414/1‑151 (MQ=255)

                     acaAAAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATAAATTATCAAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTAtt                                                                                                                            <  1:1156604/151‑1 (MQ=255)

                             aTGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAAcc                                                                                                                    >  1:843392/1‑151 (MQ=255)

                                  gAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGttt                                                                                                                >  3:152456/1‑150 (MQ=255)

                                   aaggCGGCCGATAGCGCCTGGGAGGGGCGTGCGGACGGTCGGCTTTAAAAAGATAAAAACCAGTTGGTGACGATGCCATAAATTACCGAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTa                                                                                                               <  1:339980/147‑1 (MQ=255)

                                     aGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGc                                                                                                            >  4:449200/1‑151 (MQ=255)

                                        ggTCGATGGCGCCTGGGAAGGGCGTGCGGACGGATTGCCTTATAAAGATAAAACCCAGTGGGTGACGATGGCATAAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCa                                                                                                           <  4:1198785/149‑1 (MQ=255)

                                          tCGATAGCGCCTGGGAAGGGCGGGGGGACGGTCTGCCTTAAAAAGATAAAACCCAGTTGGTGACGATGGCATCAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTg                                                                                                        <  1:845725/150‑1 (MQ=255)

                                             aaagcccttGGGAAGGGCGTGCGGAGGGTCTGCCTTAAAAAAAAAAAACCCGGTTGGTGACGATGGCTTAAATTATCAAAAAATAATCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAGCCGTTTACGCAtt                                                                                                         <  1:817574/138‑1 (MQ=255)

                                             aTAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTAt                                                                                                    >  1:995377/1‑151 (MQ=255)

                                             aTAGCGCCGGGGAAGGGCGTGGGGACGGTCTGCCTTAAAAAAAAAAAACCCAGTTGGTGACGATGGCATAAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTa                                                                                                     <  1:1773606/150‑1 (MQ=255)

                                                   ccTGGGAAGGGCGGGCGGACGGTCTGCCTTATAAAGAAAAAACCCAGTGGGTGACGATGGCATAAATTATCGAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATgcg                                                                                                 <  1:1646776/148‑1 (MQ=255)

                                                      gggAGGGGGGGGCGGACGGTCTGCCTTATAAAGAAAAAACCCAGTGGGGGCGGTGGGAATCAATTATCGAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCa                                                                                           <  3:532254/151‑1 (MQ=255)

                                                       ggAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGc                                                                                            >  3:245276/1‑149 (MQ=255)

                                                         aaGGGCGTGCGGACGGTCTGCTTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAgac                                                                                        <  2:324496/151‑1 (MQ=255)

                                                         aaGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAACCCAGTGGGTGACGAGGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAgac                                                                                        <  1:653076/151‑1 (MQ=255)

                                                         aaGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAga                                                                                         <  4:609480/150‑1 (MQ=255)

                                                           gggCGTGCGGACGGTCTGCTTTATAAAGAAAAAAACCAGTTGGTGACGAGGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAgaccg                                                                                      <  1:1021667/151‑1 (MQ=255)

                                                           gggCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCGGTTGGTGACGATGGCATCAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAgaca                                                                                       <  3:52560/150‑2 (MQ=255)

                                                                tGCGGACGGTCTGCCTTTTAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAgaccgacc                                                                                   <  1:1178430/149‑1 (MQ=255)

                                                                   ggAGGGTCTGCCTTATAAAGAAAAAAGCAAGTTGGTGAGGAGGGAATAATTTATGAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACcgacccgacc                                                                              <  3:542462/151‑1 (MQ=255)

                                                                   ggACGTTCTGCCTTAAAAAGATAAAACCCATTTGGGGACGATGGCATCAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGAccgacccga                                                                                <  3:247140/149‑1 (MQ=255)

                                                                   ggACGGTCTGCCTTATAAAGATAAAACCCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGAccgacccg                                                                                 <  1:261108/148‑1 (MQ=255)

                                                                     aCGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCg                                                                             >  1:226108/1‑150 (MQ=255)

                                                                      cGGTCTGCCTTATAAAGAAAAAACCCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGt                                                                            <  1:1795779/150‑1 (MQ=255)

                                                                       ggttggCCTTAAAAAGATAAAAACCAGTTGGTGACGATGGCACCAATTATGGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTg                                                                           <  1:719107/145‑1 (MQ=255)

                                                                         tCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGAtt                                                                        >  4:134069/1‑151 (MQ=255)

                                                                            gctttATAAAGAAAAAACCCGTTTGGTGAGGATGGCATAAATTATCAAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGAttt                                                                       <  3:397404/146‑1 (MQ=255)

                                                                            gCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTAc                                                                     >  1:487503/1‑151 (MQ=255)

                                                                             ccTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACg                                                                    >  3:428426/1‑151 (MQ=255)

                                                                             ccTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACg                                                                    >  1:1632314/1‑151 (MQ=255)

                                                                              cTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACgg                                                                   >  4:144006/1‑151 (MQ=255)

                                                                                tataAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACggg                                                                  >  1:1564670/1‑150 (MQ=255)

                                                                                 ataAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGAt                                                                <  2:514933/151‑1 (MQ=255)

                                                                                 ataAAGATAAAAACCAGTTGGTGACGATGGCATCAATTACCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGAt                                                                <  1:91506/151‑1 (MQ=255)

                                                                                   aaaGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATgg                                                              >  1:896547/1‑151 (MQ=255)

                                                                                    aaGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATggg                                                             >  1:1893242/1‑151 (MQ=255)

                                                                                    aaGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATggg                                                             >  4:1258355/1‑151 (MQ=255)

                                                                                    aaGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATggg                                                             >  2:377453/1‑151 (MQ=255)

                                                                                    aaGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATggg                                                             <  4:1026302/151‑1 (MQ=255)

                                                                                     aGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATggg                                                             >  1:906164/1‑150 (MQ=255)

                                                                                     aGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATggg                                                             >  4:874758/1‑150 (MQ=255)

                                                                                      gATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATggg                                                             >  4:46364/1‑149 (MQ=255)

                                                                                       aTAAAAACCAGTGGGTGACGAGGGCATCAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGgaga                                                          <  4:759970/151‑1 (MQ=255)

                                                                                         aaaacCCAGTTGGTGACGATGGCATCAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGgagat                                                         <  1:980472/150‑2 (MQ=255)

                                                                                          aaaaCCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCg                                                       >  4:1794710/1‑151 (MQ=255)

                                                                                           aaaCCAGTTGGTGACGATGGCATCAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGt                                                      <  4:1124665/151‑1 (MQ=255)

                                                                                            aaCCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGtt                                                     >  4:1568000/1‑151 (MQ=255)

                                                                                             aCCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGtt                                                     >  4:1521084/1‑150 (MQ=255)

                                                                                               cAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTtat                                                   >  2:2397/1‑150 (MQ=255)

                                                                                               cAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTtat                                                   >  1:716047/1‑150 (MQ=255)

                                                                                                         cGATGGCATCAATTATCGAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAAc                                         <  2:537220/150‑1 (MQ=255)

                                                                                                           aTGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACttt                                      >  4:1038108/1‑151 (MQ=255)

                                                                                                                 tCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTg                                >  2:437330/1‑151 (MQ=255)

                                                                                                                  cAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGc                               >  4:1225524/1‑151 (MQ=255)

                                                                                                                   aaTTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTg                                <  4:538928/149‑1 (MQ=255)

                                                                                                                   aaTTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGc                               >  1:1461165/1‑150 (MQ=255)

                                                                                                                   aaTTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGc                               >  1:760304/1‑150 (MQ=255)

                                                                                                                   aaTTATAAAAAAGAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCa                              <  4:1252342/151‑1 (MQ=255)

                                                                                                                     ttATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGc                               >  1:15674/1‑148 (MQ=255)

                                                                                                                       aTCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGAcc                          <  4:1190379/151‑1 (MQ=255)

                                                                                                                       aTCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGAc                           <  3:197979/150‑1 (MQ=255)

                                                                                                                         cGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTg                        >  4:1220518/1‑151 (MQ=255)

                                                                                                                          gAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTgg                       >  4:20116/1‑151 (MQ=255)

                                                                                                                           aaaaaGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCt                         <  1:30268/148‑1 (MQ=255)

                                                                                                                           aaaaaGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGa                      <  1:103017/151‑1 (MQ=255)

                                                                                                                              aaGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAAc                   >  1:1116597/1‑151 (MQ=255)

                                                                                                                              aaGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAAc                   >  2:143944/1‑151 (MQ=255)

                                                                                                                              aaGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAAc                   <  2:250923/151‑1 (MQ=255)

                                                                                                                               aGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACg                  >  4:666389/1‑151 (MQ=255)

                                                                                                                                gAAACCGCCGTTGCCTGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACg                  >  4:584360/1‑150 (MQ=255)

                                                                                                                                  aaCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCg               >  4:938046/1‑151 (MQ=255)

                                                                                                                                  aaCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCg               >  1:889676/1‑151 (MQ=255)

                                                                                                                                  aaCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCg               <  4:1372661/151‑1 (MQ=255)

                                                                                                                                    ccgccgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGAc             <  4:1139979/151‑1 (MQ=255)

                                                                                                                                     cgccgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACa            <  4:1520633/151‑1 (MQ=255)

                                                                                                                                       ccgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGc          >  4:1007884/1‑151 (MQ=255)

                                                                                                                                        cgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCg         >  1:634249/1‑151 (MQ=255)

                                                                                                                                         gTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGt        >  4:268539/1‑151 (MQ=255)

                                                                                                                                           tGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGta       <  2:152069/150‑1 (MQ=255)

                                                                                                                                           tGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGta       <  4:1791938/150‑1 (MQ=255)

                                                                                                                                              cAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAac   <  4:1433026/151‑1 (MQ=255)

                                                                                                                                               aGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAac   >  3:328349/1‑150 (MQ=255)

TCGCGTTACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACA  >  NC_000913/1154635‑1154928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`