BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,158,225	C→T	L119L (CTC→CTT)	ptsG →	fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,158,225	0	C	T	100.0%	510.4 / NA	131	L119L (CTC→CTT)	ptsG	fused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (59/72); total (59/72)

Only 100 of 131 total aligned reads displayed.

TTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGC  >  NC_000913/1158079‑1158370

tttACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTgg                                                                                                                                                 >  4:1778616/1‑149 (MQ=255)

 ttACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGa                                                                                                                                                <  1:628074/149‑1 (MQ=255)

 ttACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAgg                                                                                                                                              >  1:1095653/1‑151 (MQ=255)

 ttACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAg                                                                                                                                               <  3:279001/150‑1 (MQ=255)

  tACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAgg                                                                                                                                              <  1:778448/150‑1 (MQ=255)

   aCCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAggg                                                                                                                                             >  4:1472508/1‑150 (MQ=255)

    ccAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGAt                                                                                                                                           <  4:227648/151‑1 (MQ=255)

       aTAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTAt                                                                                                                                        >  1:723247/1‑151 (MQ=255)

         aaCGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTAtct                                                                                                                                      <  4:920904/151‑1 (MQ=255)

           cGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTcc                                                                                                                                    >  4:1337318/1‑151 (MQ=255)

            gATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCg                                                                                                                                   <  1:1565114/151‑1 (MQ=255)

             aTGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAATCGCCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCg                                                                                                                                   <  1:1554995/150‑1 (MQ=255)

             aTGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCgg                                                                                                                                  >  4:1918621/1‑151 (MQ=255)

              tGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCgg                                                                                                                                  <  1:891590/150‑1 (MQ=255)

                gCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGc                                                                                                                               <  4:503651/151‑1 (MQ=255)

                    aTCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATc                                                                                                                           >  1:574086/1‑151 (MQ=255)

                    aTCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATc                                                                                                                           <  4:1782608/151‑1 (MQ=255)

                      ccGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGAt                                                                                                                            <  3:416486/148‑1 (MQ=255)

                      ccGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGc                                                                                                                         <  1:464403/151‑1 (MQ=255)

                       cgcgCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGc                                                                                                                         >  2:511068/1‑150 (MQ=255)

                          gcTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCg                                                                                                                     >  4:1258216/1‑151 (MQ=255)

                            tGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGt                                                                                                                    >  1:1217032/1‑150 (MQ=255)

                              gCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTAc                                                                                                                  >  1:1394547/1‑150 (MQ=255)

                                cGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACAt                                                                                                                <  4:1282484/150‑1 (MQ=255)

                                cGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATg                                                                                                               >  1:240593/1‑151 (MQ=255)

                                  cAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGtt                                                                                                             <  4:855362/151‑1 (MQ=255)

                                  cAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGtt                                                                                                             <  4:266824/151‑1 (MQ=255)

                                       gttgCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAAcc                                                                                                        >  4:1453382/1‑151 (MQ=255)

                                       gttgCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAAcc                                                                                                        <  1:1345645/151‑1 (MQ=255)

                                         tgCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGt                                                                                                      >  4:896234/1‑151 (MQ=255)

                                          gCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGtt                                                                                                     <  4:551446/151‑1 (MQ=255)

                                          gCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGtt                                                                                                     >  1:1180335/1‑151 (MQ=255)

                                              aTGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCt                                                                                                  <  1:1450320/150‑1 (MQ=255)

                                              aTGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCt                                                                                                  <  1:1244050/150‑1 (MQ=255)

                                               tGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTAc                                                                                                >  1:1030785/1‑151 (MQ=255)

                                                     catGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTAt                                                                                           <  4:1293218/150‑1 (MQ=255)

                                                      atGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTa                                                                                         <  1:1524148/151‑1 (MQ=255)

                                                            aaaaCCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGc                                                                                   <  2:46635/151‑1 (MQ=255)

                                                               aCCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTg                                                                                >  4:893086/1‑151 (MQ=255)

                                                               aCCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTg                                                                                <  1:1492232/151‑1 (MQ=255)

                                                                ccATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGa                                                                               <  1:650346/151‑1 (MQ=255)

                                                                    ggCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTa                                                                            <  1:1307891/150‑1 (MQ=255)

                                                                    ggCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTAt                                                                           <  1:324949/151‑1 (MQ=255)

                                                                    ggCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTAt                                                                           <  4:615311/151‑1 (MQ=255)

                                                                     gCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATc                                                                          >  1:629550/1‑151 (MQ=255)

                                                                      ccGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCt                                                                         <  1:4838/151‑1 (MQ=255)

                                                                        gTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTg                                                                       >  1:678430/1‑151 (MQ=255)

                                                                          ggTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTgg                                                                      <  1:1447920/150‑1 (MQ=255)

                                                                          ggTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGc                                                                     <  4:1050318/151‑1 (MQ=255)

                                                                           gTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGct                                                                    <  1:1471917/151‑1 (MQ=255)

                                                                             tGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGcttc                                                                  >  1:223876/1‑151 (MQ=255)

                                                                             tGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGctt                                                                   <  1:1010115/150‑1 (MQ=255)

                                                                              gcgcCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAAAACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGcttct                                                                 <  4:113880/151‑1 (MQ=255)

                                                                               cgcCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGcttctt                                                                >  1:971201/1‑151 (MQ=255)

                                                                               cgcCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGcttctt                                                                >  2:322402/1‑151 (MQ=255)

                                                                                 ccACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTg                                                              <  4:928761/151‑1 (MQ=255)

                                                                                 ccACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTg                                                              >  1:606448/1‑151 (MQ=255)

                                                                                   aCTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGcc                                                            >  4:1674822/1‑151 (MQ=255)

                                                                                      ggTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCg                                                           <  4:1413727/149‑1 (MQ=255)

                                                                                      ggTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGt                                                         <  4:1405789/151‑1 (MQ=255)

                                                                                       gTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTa                                                        <  2:9598/151‑1 (MQ=255)

                                                                                         aCTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTaaa                                                      <  4:109671/151‑1 (MQ=255)

                                                                                         aCTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTaa                                                       >  4:1028890/1‑150 (MQ=255)

                                                                                         aCTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTaa                                                       <  1:1030284/150‑1 (MQ=255)

                                                                                            gCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGTCGGTAAACg                                                    <  4:903645/150‑1 (MQ=255)

                                                                                            gCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAAc                                                     <  4:1222291/149‑1 (MQ=255)

                                                                                            gCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAAc                                                     <  4:720516/149‑1 (MQ=255)

                                                                                               tttACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGAATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCtt                                                 >  1:335843/1‑150 (MQ=255)

                                                                                                  aCCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTtgtg                                             >  4:410998/1‑151 (MQ=255)

                                                                                                      gctgAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCg                                          <  2:43453/150‑1 (MQ=255)

                                                                                                      gctgAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCg                                          <  1:97292/150‑1 (MQ=255)

                                                                                                         gaagaaATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGAt                                        <  3:39145/149‑1 (MQ=255)

                                                                                                          aagaaATCGCCTCTAAACACCTGGCGGATGCTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATc                                       <  4:234111/149‑1 (MQ=255)

                                                                                                          aagaaATCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCAt                                     <  4:723563/151‑1 (MQ=255)

                                                                                                              aaTCGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTct                                 >  2:401761/1‑151 (MQ=255)

                                                                                                                 cGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTctgg                               <  1:1834477/150‑1 (MQ=255)

                                                                                                                 cGCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTctgg                               <  1:1017547/150‑1 (MQ=255)

                                                                                                                  gCCTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTctggcc                             <  1:340988/151‑1 (MQ=255)

                                                                                                                   ccTCTAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTctggcct                            <  4:220526/151‑1 (MQ=255)

                                                                                                                    ctctAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCACAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTctggcctg                           >  4:272933/1‑151 (MQ=255)

                                                                                                                     tctAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTctggcctgg                          >  1:1845470/1‑151 (MQ=255)

                                                                                                                       tAAACACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCt                        >  4:1042817/1‑151 (MQ=255)

                                                                                                                        aaaCACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTg                       <  3:381896/151‑1 (MQ=255)

                                                                                                                         aaCACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGc                      >  3:72939/1‑151 (MQ=255)

                                                                                                                         aaCACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGc                      >  1:1271817/1‑151 (MQ=255)

                                                                                                                         aaCACCTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGc                      >  1:1859695/1‑151 (MQ=255)

                                                                                                                             ccTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATc                  <  2:128945/151‑1 (MQ=255)

                                                                                                                              cTGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCt                 >  4:1149197/1‑151 (MQ=255)

                                                                                                                               tGGCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCtt                >  4:814855/1‑151 (MQ=255)

                                                                                                                                 gCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCttt               >  4:1662098/1‑150 (MQ=255)

                                                                                                                                 gCGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCttt               >  2:57700/1‑150 (MQ=255)

                                                                                                                                  cGGATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTAc             <  2:311586/151‑1 (MQ=255)

                                                                                                                                   ggATACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACt            >  4:1709534/1‑151 (MQ=255)

                                                                                                                                     aTACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTgg          >  4:1201834/1‑151 (MQ=255)

                                                                                                                                     aTACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTgg          <  1:1081903/151‑1 (MQ=255)

                                                                                                                                      tACTGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCCGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCATGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTg           >  1:219164/1‑149 (MQ=255)

                                                                                                                                         tGGCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGtt      >  1:559912/1‑151 (MQ=255)

                                                                                                                                           gCGTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTtgt    >  1:285366/1‑151 (MQ=255)

                                                                                                                                             gTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGc  >  1:480423/1‑151 (MQ=255)

                                                                                                                                             gTACTTGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGc  <  4:249034/151‑1 (MQ=255)

TTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGC  >  NC_000913/1158079‑1158370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`