Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,169,759 C→T D206N (GAT→AAT)  ldtC ← L,D‑transpeptidase linking Lpp to murein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,169,7590CT99.2% 492.6 / ‑4.2 127D206N (GAT→AAT) ldtCL,D‑transpeptidase linking Lpp to murein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (60/66);  total (61/66)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.88e-01

Only 100 of 127 total aligned reads displayed.

CGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGG  >  NC_000913/1169611‑1169906
                                                                                                                                                    |                                                                                                                                                   
cgcgCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTgg                                                                                                                                                   >  1:1535453/1‑151 (MQ=255)
 gcgcACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGc                                                                                                                                                  <  4:1608526/151‑1 (MQ=255)
 gcgcACCGTTTGGATCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGc                                                                                                                                                  >  4:327557/1‑151 (MQ=255)
    cACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGtt                                                                                                                                               >  4:1106712/1‑151 (MQ=255)
    cACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGt                                                                                                                                                <  4:1064270/150‑1 (MQ=255)
     aCCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTc                                                                                                                                              <  2:263494/151‑1 (MQ=255)
       cGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGt                                                                                                                                            <  4:1851321/151‑1 (MQ=255)
       cGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGt                                                                                                                                            >  1:665626/1‑151 (MQ=255)
        gTTTGGTTCGGCAGAGCCTATTATCGGAGTGTTGATGATATTCCCGTCGGTGCCTGGTGTGAACTGGCTAAAGAGTGTTTTCATATCAACATCCCGCAGACGCATCCAGCCCTAACTCACCCCCAGGACAATGCCCAAATTAGCGTTCGTa                                                                                                                                           <  3:98287/151‑1 (MQ=255)
          ttGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTAc                                                                                                                                          <  3:281728/150‑1 (MQ=255)
            ggTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCAt                                                                                                                                       >  1:108120/1‑151 (MQ=255)
             gTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATg                                                                                                                                      <  4:578970/151‑1 (MQ=255)
             gTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATg                                                                                                                                      <  1:1449534/151‑1 (MQ=255)
              ttCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATg                                                                                                                                      >  1:612087/1‑150 (MQ=255)
                 ggCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGc                                                                                                                                  <  4:1502714/151‑1 (MQ=255)
                  gCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGc                                                                                                                                  >  1:314620/1‑150 (MQ=255)
                  gCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCa                                                                                                                                 <  4:773081/151‑1 (MQ=255)
                       gaCTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATaa                                                                                                                            <  1:1583315/151‑1 (MQ=255)
                       gaCTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATaa                                                                                                                            <  1:368494/151‑1 (MQ=255)
                        aCTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATaa                                                                                                                            >  3:218280/1‑150 (MQ=255)
                           tttATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAAcg                                                                                                                         <  2:101134/150‑1 (MQ=255)
                            ttATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAAcgcc                                                                                                                       >  1:1023589/1‑151 (MQ=255)
                                 ggAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCAt                                                                                                                  >  4:263578/1‑151 (MQ=255)
                                    gtgtTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAgg                                                                                                               <  2:40854/151‑1 (MQ=255)
                                    gtgtTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAgg                                                                                                               <  2:416273/151‑1 (MQ=255)
                                     tgtTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGgc                                                                                                              >  2:145589/1‑151 (MQ=255)
                                     tgtTGATGATATTCACTTTCGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGgc                                                                                                              >  4:875409/1‑151 (MQ=255)
                                      gtTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGgcc                                                                                                             <  4:281611/151‑1 (MQ=255)
                                        tgatgaTATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGgccg                                                                                                            <  1:922494/150‑1 (MQ=255)
                                         gatgatATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGgccgcc                                                                                                          <  1:905695/151‑1 (MQ=255)
                                            gatATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGa                                                                                                       <  4:1928403/151‑1 (MQ=255)
                                            gatATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGa                                                                                                       <  3:166790/151‑1 (MQ=255)
                                            gatATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGa                                                                                                       <  4:501253/151‑1 (MQ=255)
                                             atatTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAc                                                                                                      <  4:477180/151‑1 (MQ=255)
                                             atatTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAc                                                                                                      <  4:1062176/151‑1 (MQ=255)
                                               atTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACg                                                                                                     <  2:247631/150‑1 (MQ=255)
                                               atTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGa                                                                                                    <  3:134187/151‑1 (MQ=255)
                                                ttCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGa                                                                                                    >  4:333582/1‑150 (MQ=255)
                                                 tCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAAt                                                                                                  >  4:389401/1‑151 (MQ=255)
                                                       tGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCatg                                                                                            >  1:1774602/1‑151 (MQ=255)
                                                        ggTGCCTGGGTTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTCCCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGTCCGCCAGACGAATCGCatg                                                                                            <  1:833040/150‑1 (MQ=255)
                                                         gTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCatgat                                                                                          >  1:1874001/1‑151 (MQ=255)
                                                           gCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCatgatg                                                                                         <  1:1484824/150‑1 (MQ=255)
                                                           gCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCatgatc                                                                                         <  1:1045461/150‑2 (MQ=255)
                                                           gCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATgg                                                                                        <  2:435265/151‑1 (MQ=255)
                                                            ccTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGc                                                                                       >  3:516975/1‑151 (MQ=255)
                                                                gggTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCAt                                                                                   >  4:1001748/1‑151 (MQ=255)
                                                                 ggTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCAtt                                                                                  <  4:238613/151‑1 (MQ=255)
                                                                   tGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTgg                                                                                >  1:1025380/1‑151 (MQ=255)
                                                                   tGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTgg                                                                                >  4:1401220/1‑151 (MQ=255)
                                                                    gACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTgg                                                                                <  1:1023078/150‑1 (MQ=255)
                                                                     aCCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGt                                                                              <  4:624783/151‑1 (MQ=255)
                                                                     aCCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGt                                                                              <  4:6182/151‑1 (MQ=255)
                                                                       cTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGtt                                                                             >  4:1264829/1‑150 (MQ=255)
                                                                        tGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAGGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGtt                                                                             <  1:317882/149‑1 (MQ=255)
                                                                           cTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTAtcc                                                                         >  1:750132/1‑150 (MQ=255)
                                                                                gcgtgtTTTGATATCGTCATCCCGCAGACGACTACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACAATGAAGCAAATAAACGCCGCCATAGGCCGACAGACGAATCGCATGATGGCCCATTGGGTTAtccagtc                                                                     >  3:85719/3‑149 (MQ=255)
                                                                                gagTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTAtccagtcc                                                                    >  4:1063475/1‑150 (MQ=255)
                                                                                   tgtTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGc                                                                 <  1:355625/150‑1 (MQ=255)
                                                                                    gtTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGcct                                                               <  3:419508/151‑2 (MQ=255)
                                                                                     ttttGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCgg                                                              >  1:103434/1‑151 (MQ=255)
                                                                                      tttGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCgg                                                              <  2:16622/150‑1 (MQ=255)
                                                                                       ttGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGc                                                             <  1:1461647/150‑1 (MQ=255)
                                                                                                tCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGc                                                     >  1:1772525/1‑149 (MQ=255)
                                                                                                tCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGc                                                     >  4:504124/1‑149 (MQ=255)
                                                                                                 cATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAgg                                                  >  1:1673894/1‑151 (MQ=255)
                                                                                                 cATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAgg                                                  >  1:516358/1‑151 (MQ=255)
                                                                                                  aTCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGc                                                 >  1:1697077/1‑151 (MQ=255)
                                                                                                      cGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACt                                             <  4:1152322/151‑1 (MQ=255)
                                                                                                      cGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACt                                             >  4:238650/1‑151 (MQ=255)
                                                                                                       gCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTc                                            >  4:208065/1‑151 (MQ=255)
                                                                                                           aCGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAAtt                                        <  1:1188654/151‑1 (MQ=255)
                                                                                                             gAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTcc                                      >  2:487784/1‑151 (MQ=255)
                                                                                                             gAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTcc                                      >  3:412828/1‑151 (MQ=255)
                                                                                                                 aCAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCATTCCAGCCGGCACTACCGCAGGCAACGCAATTCCCtgt                                  <  1:1312406/151‑1 (MQ=255)
                                                                                                                 aCAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCtgt                                  >  4:1561312/1‑151 (MQ=255)
                                                                                                                     ccAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCt                               <  4:1557629/150‑1 (MQ=255)
                                                                                                                      cAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCttt                             >  1:667875/1‑151 (MQ=255)
                                                                                                                        gAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTtat                           >  4:772477/1‑151 (MQ=255)
                                                                                                                         aaCTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTtata                          <  1:1559077/151‑1 (MQ=255)
                                                                                                                         aaCTTACCCGAATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTtata                          <  1:1047121/151‑1 (MQ=255)
                                                                                                                              aCCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACggg                     >  4:875767/1‑151 (MQ=255)
                                                                                                                              aCCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACgg                      <  4:835415/150‑1 (MQ=255)
                                                                                                                               cccGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACgg                      <  3:394373/149‑1 (MQ=255)
                                                                                                                               cccGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACg                       <  1:1869031/148‑1 (MQ=255)
                                                                                                                               cccGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGgc                    <  1:1849798/151‑1 (MQ=255)
                                                                                                                                ccGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGgcg                   >  2:380707/1‑151 (MQ=255)
                                                                                                                                ccGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGgcg                   <  4:254021/151‑1 (MQ=255)
                                                                                                                                 cGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGgcgc                  <  2:275299/151‑1 (MQ=255)
                                                                                                                                  gCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGcgcg                 >  2:395213/1‑151 (MQ=255)
                                                                                                                                    aTGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCgg                <  1:173162/150‑1 (MQ=255)
                                                                                                                                      gCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGAt              <  2:226179/150‑1 (MQ=255)
                                                                                                                                       ccAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGt            >  1:1764790/1‑151 (MQ=255)
                                                                                                                                        cAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGtt           >  2:434618/1‑151 (MQ=255)
                                                                                                                                        cAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGtt           >  1:1503724/1‑151 (MQ=255)
                                                                                                                                         aaTGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGttt          >  1:225990/1‑151 (MQ=255)
                                                                                                                                          aTGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTg         <  4:555553/151‑1 (MQ=255)
                                                                                                                                                aaaTTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTg   >  4:809939/1‑151 (MQ=255)
                                                                                                                                                 aaTTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTgg  >  4:1259830/1‑151 (MQ=255)
                                                                                                                                                 aaTTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTgg  <  4:820568/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGG  >  NC_000913/1169611‑1169906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑