Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 143,721 T→C V7A (GTG→GCG)  yadH → putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913143,7210TC99.3% 520.9 / ‑4.1 140V7A (GTG→GCG) yadHputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (66/73);  minor base A (1/0);  total (67/73)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.29e-01

Only 100 of 143 total aligned reads displayed.

CAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTT  >  NC_000913/143574‑143868
                                                                                                                                                   |                                                                                                                                                   
cAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGggcggc                                                                                                                                                  >  1:391610/1‑151 (MQ=255)
   gggATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCt                                                                                                                                               >  1:976439/1‑151 (MQ=255)
         aaCAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAg                                                                                                                                         <  3:519991/151‑1 (MQ=255)
          aCAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGc                                                                                                                                        >  4:1843213/1‑151 (MQ=255)
           cAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGc                                                                                                                                        >  2:194514/1‑150 (MQ=255)
              cGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCt                                                                                                                                    >  1:1771529/1‑151 (MQ=255)
               gTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTg                                                                                                                                   >  1:1623049/1‑151 (MQ=255)
                tATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTgg                                                                                                                                  >  1:235072/1‑151 (MQ=255)
                 aTTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTggg                                                                                                                                 >  4:959430/1‑151 (MQ=255)
                 aTTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTggg                                                                                                                                 <  4:780527/151‑1 (MQ=255)
                     aCGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGa                                                                                                                              >  4:911778/1‑150 (MQ=255)
                      cGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGaaa                                                                                                                            <  1:232245/151‑1 (MQ=255)
                      cGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGaa                                                                                                                             >  4:538231/1‑150 (MQ=255)
                       gCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAag                                                                                                                           <  1:1845514/151‑1 (MQ=255)
                        cAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGaa                                                                                                                             >  1:1812782/1‑148 (MQ=255)
                        cAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAaga                                                                                                                          <  1:785240/151‑1 (MQ=255)
                           ttAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAag                                                                                                                           >  2:445468/1‑147 (MQ=255)
                            tAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAag                                                                                                                           >  1:700670/1‑146 (MQ=255)
                            tAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGAt                                                                                                                       <  1:489017/150‑1 (MQ=255)
                             aaGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATcc                                                                                                                     >  4:167988/1‑151 (MQ=255)
                               gTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCAt                                                                                                                   <  1:461951/151‑1 (MQ=255)
                                tGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATc                                                                                                                  >  3:483780/1‑151 (MQ=255)
                                 gAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATc                                                                                                                  <  4:1377387/150‑1 (MQ=255)
                                 gAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGAGCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGAGCGAAAGTGATGCATc                                                                                                                  <  1:758649/150‑1 (MQ=255)
                                  aGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGc                                                                                                                >  4:259368/1‑151 (MQ=255)
                                  aGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGc                                                                                                                <  1:1910107/151‑1 (MQ=255)
                                  aGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGc                                                                                                                >  1:124823/1‑151 (MQ=255)
                                  aGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGc                                                                                                                <  4:1048886/151‑1 (MQ=255)
                                   gCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCt                                                                                                               <  4:453765/151‑1 (MQ=255)
                                   gCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCt                                                                                                               >  2:388819/1‑151 (MQ=255)
                                   gCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCt                                                                                                               >  1:1523269/1‑151 (MQ=255)
                                        gCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATg                                                                                                          <  4:1177350/151‑1 (MQ=255)
                                               ggTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCt                                                                                                   <  2:336432/151‑1 (MQ=255)
                                               ggTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCt                                                                                                   <  1:650792/151‑1 (MQ=255)
                                                gTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTg                                                                                                  <  3:286492/151‑1 (MQ=255)
                                                gTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTg                                                                                                  >  3:212623/1‑151 (MQ=255)
                                                  aTTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTgg                                                                                                 <  4:533772/150‑1 (MQ=255)
                                                     aaGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGc                                                                                             >  1:1488045/1‑151 (MQ=255)
                                                      aGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCa                                                                                            <  4:1678437/151‑1 (MQ=255)
                                                        tATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGc                                                                                             >  1:1195192/1‑148 (MQ=255)
                                                         aTGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGa                                                                                          >  4:1222685/1‑150 (MQ=255)
                                                         aTGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGAc                                                                                         >  2:329978/1‑151 (MQ=255)
                                                           gCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACg                                                                                        <  4:965612/150‑1 (MQ=255)
                                                            cGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCt                                                                                      >  2:275470/1‑151 (MQ=255)
                                                             gTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTg                                                                                     <  4:570219/151‑1 (MQ=255)
                                                               aaCAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCTGACGCTg                                                                                     >  3:478687/1‑149 (MQ=255)
                                                                   aaGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTgccg                                                                               >  2:284389/1‑151 (MQ=255)
                                                                     gCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTgccgcc                                                                             >  1:1547392/1‑151 (MQ=255)
                                                                     gCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTgccgcc                                                                             <  2:409386/151‑1 (MQ=255)
                                                                            gTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGtcatc                                                                      >  3:252725/1‑151 (MQ=255)
                                                                             tCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGtcatc                                                                      >  4:1453605/1‑150 (MQ=255)
                                                                              cTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCAc                                                                    >  1:1272663/1‑151 (MQ=255)
                                                                                ggAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCAc                                                                    <  4:678102/149‑1 (MQ=255)
                                                                                ggAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCAc                                                                    <  4:188221/149‑1 (MQ=255)
                                                                                ggAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCa                                                                  <  4:1234307/151‑1 (MQ=255)
                                                                                 gAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCAt                                                                 <  4:173570/151‑1 (MQ=255)
                                                                                   agagCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGa                                                               <  1:540046/151‑1 (MQ=255)
                                                                                    gagCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGAc                                                              <  4:840236/151‑1 (MQ=255)
                                                                                       cTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCct                                                           >  4:533140/1‑151 (MQ=255)
                                                                                         gtttgtttCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCct                                                           <  4:1665478/149‑1 (MQ=255)
                                                                                         gtttgtttCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCct                                                           <  4:1337261/149‑1 (MQ=255)
                                                                                              tttCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCctttactt                                                     <  1:1653243/150‑1 (MQ=255)
                                                                                                tCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCctttacttta                                                   <  1:1118438/150‑1 (MQ=255)
                                                                                                 cACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTttatt                                                 >  4:1784647/1‑151 (MQ=255)
                                                                                                 cACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTttat                                                  <  4:1837331/150‑1 (MQ=255)
                                                                                                     ggTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCt                                             >  3:358058/1‑151 (MQ=255)
                                                                                                      gTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCtt                                            >  1:56963/1‑151 (MQ=255)
                                                                                                      gTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCtt                                            <  2:3201/151‑1 (MQ=255)
                                                                                                        tAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTc                                           <  1:1082343/150‑1 (MQ=255)
                                                                                                         aaTGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCgg                                         >  1:969007/1‑151 (MQ=255)
                                                                                                         aaTGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCgg                                         >  3:503043/1‑151 (MQ=255)
                                                                                                         aaTGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCg                                          <  1:1193968/150‑1 (MQ=255)
                                                                                                         aaTGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCg                                          <  1:791366/150‑1 (MQ=255)
                                                                                                          aTGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGt                                        <  1:1721534/151‑1 (MQ=255)
                                                                                                          aTGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGt                                        <  2:503063/151‑1 (MQ=255)
                                                                                                            gAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTa                                       >  1:953458/1‑150 (MQ=255)
                                                                                                             aaaaaCAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAAc                                     <  1:284834/151‑1 (MQ=255)
                                                                                                               aaaCAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCt                                   >  2:437505/1‑151 (MQ=255)
                                                                                                               aaaCAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCt                                   >  4:271154/1‑151 (MQ=255)
                                                                                                                aaCAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTg                                  <  4:1876428/151‑1 (MQ=255)
                                                                                                                  cAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGAt                                <  4:1634016/151‑1 (MQ=255)
                                                                                                                   aaGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGAtt                               <  4:1523870/151‑1 (MQ=255)
                                                                                                                       agaTCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGtt                           >  4:394273/1‑151 (MQ=255)
                                                                                                                        gaTCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTTGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTc                          <  3:397774/151‑1 (MQ=255)
                                                                                                                              gcATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTAt                    >  4:1192099/1‑151 (MQ=255)
                                                                                                                              gcATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTAt                    >  1:116873/1‑151 (MQ=255)
                                                                                                                                atgatgCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTg                  <  4:1241169/151‑1 (MQ=255)
                                                                                                                                  gatgCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGc                >  4:754895/1‑151 (MQ=255)
                                                                                                                                    tgCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCg               >  4:1067096/1‑150 (MQ=255)
                                                                                                                                     gCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGa              <  4:1507352/150‑1 (MQ=255)
                                                                                                                                         cTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGc         >  1:499073/1‑151 (MQ=255)
                                                                                                                                         cTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGc         >  4:1360375/1‑151 (MQ=255)
                                                                                                                                         cTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGc         >  4:774994/1‑151 (MQ=255)
                                                                                                                                           ttACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATg          <  4:1866072/148‑1 (MQ=255)
                                                                                                                                           ttACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCa        <  4:56978/150‑1 (MQ=255)
                                                                                                                                           ttACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCa        <  4:700972/150‑1 (MQ=255)
                                                                                                                                           ttACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCAt       >  4:1365575/1‑151 (MQ=255)
                                                                                                                                            tACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCAt       <  4:177064/150‑1 (MQ=255)
                                                                                                                                            tACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATg      >  4:729924/1‑151 (MQ=255)
                                                                                                                                                gggCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCtt  >  4:710828/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTT  >  NC_000913/143574‑143868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: