Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,308,170 T→C D159G (GAC→GGC)  clsA ← cardiolipin synthase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,308,1700TC99.2% 462.0 / ‑4.5 129D159G (GAC→GGC) clsAcardiolipin synthase 1
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (70/58);  total (71/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

Only 100 of 131 total aligned reads displayed.

CTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGAC  >  NC_000913/1308022‑1308317
                                                                                                                                                       |                                                                                                                                                   
  ttAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGccc                                                                                                                                                   <  4:298275/149‑1 (MQ=255)
   tAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACCCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGc                                                                                                                                                 <  1:1796900/150‑1 (MQ=255)
    aaGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGcc                                                                                                                                                >  1:1730022/1‑150 (MQ=255)
    aaGCCTTCGACCACTCAAATCCCGGCATTACGCATTAGCTCGGGCCACGGGCGGCGAAAAAAAGCCACCCTCCCGGCGGAGTCGGGCATCAATCGGCAAGGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCCCCTGGCCCGcct                                                                                                                                               <  3:389690/151‑2 (MQ=255)
         ttCGCCCCCTTCAATCCCGGCATTACCCATTAGCCCGGGCCAGGGGCGGGGAAAAAAACCCACCCCCCCGGGGGAGCCGAGCATAAATCGGAAATGAATCCCGCGCCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCAt                                                                                                                                              <  4:113335/147‑1 (MQ=255)
         ttCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATgccg                                                                                                                                          >  2:443009/1‑151 (MQ=255)
         ttCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATgccg                                                                                                                                          >  4:7363/1‑151 (MQ=255)
         ttCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATgcc                                                                                                                                           >  3:375332/1‑150 (MQ=255)
         ttCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATgcc                                                                                                                                           >  4:1146853/1‑150 (MQ=255)
            gACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATgccgcc                                                                                                                                        >  4:160669/1‑150 (MQ=255)
                  ttCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGc                                                                                                                                 >  1:1880194/1‑151 (MQ=255)
                  ttCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGc                                                                                                                                 >  4:788498/1‑151 (MQ=255)
                  tcaaaccccGGCATCCCCCATTAGCCCGGCCCACGGGCGGGGAAAAAAACCCACCCCCCGGGGGGAGGCGGGAATCAATCGAAAATGAATGCCGCGCCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCt                                                                                                                                   <  1:1418509/142‑1 (MQ=255)
                    cAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGcc                                                                                                                                >  1:402499/1‑150 (MQ=255)
                     aaTCCCGGAATTCCGCATTAGCCGGGCCCCGGGCTTGGAAAAAAAGCCCCCCCCCCGGGCGGGGCCGAGCATAAACCGGAAATGAGTGCCGCGTCCCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCa                                                                                                                               <  1:291057/150‑1 (MQ=255)
                     aaTACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGcc                                                                                                                                >  4:1612742/1‑149 (MQ=255)
                      atcccGGCTTTCCGAATTAGCGGGGCCCCGGGGCGGGGAAAAAAACCCACCCCCCCGGCGGAGTCGAGCATCAACCGGCAATGAAGGCCGCGGCGCGCGGCCGCCATTAATGATTCAGCCCCCTGGCCCGCCATGCCGCCGGGCTGCCAGa                                                                                                                             <  1:1621826/148‑1 (MQ=255)
                      aTACCGGCATTACGCATTAGCTCGGTCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCTTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCGGGGCTGCAAg                                                                                                                              >  3:480034/1‑150 (MQ=255)
                      aTACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGcc                                                                                                                                >  4:1529615/1‑148 (MQ=255)
                      aTACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGcc                                                                                                                                >  4:1179137/1‑148 (MQ=255)
                        aCCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGat                                                                                                                            >  3:104770/1‑150 (MQ=255)
                         ccGGCATTACGCATTAGCTCGGCCCACGGGCTGGGAAAAAAAGCCACCCCCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGatat                                                                                                                          <  4:178227/151‑1 (MQ=255)
                          cGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGatat                                                                                                                          >  4:1206983/1‑150 (MQ=255)
                                 aCGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACAcc                                                                                                                  >  2:73875/1‑151 (MQ=255)
                                  cGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACAcc                                                                                                                  >  2:293299/1‑150 (MQ=255)
                                      ttAGCTCGGGCCAGGGGCGGGGAAAAAACCCCCCCCCCCGGGGGGAGCCGAGCATAAACGGGAAAGGAATGCCGCGCCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCa                                                                                                                 <  1:1155233/147‑1 (MQ=255)
                                       tAGCCCGGGCCACGGGCTGCGAAAAAAACCCACCCCCCCGGCGGGGTCGAGAATAAATCGGAAATAAACCCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGTCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCa                                                                                                                 <  4:1857790/146‑1 (MQ=255)
                                        aGCCCGGGCCCAGGGCCAGGGAAAAAACGCCACACCCCCGGCGGATGAGGCCACGAATGGGCATTAAGTCCGTGGTCCCGCGGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCAtcgc                                                                                                             <  1:1731490/150‑3 (MQ=255)
                                         gCTGGGGCCAGGGGCTGGGAAAAAAACCCACACCCCCGGCGGGGTCGAGCAACAACCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCa                                                                                                                 <  1:629899/144‑1 (MQ=255)
                                           tCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAatat                                                                                                        >  4:744126/1‑151 (MQ=255)
                                             ggcccccGGGCTGCGAAAAAAACCCCCCCCCCCGGCGGAGTCGACCATAAACCGGCAATAAAGGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAatat                                                                                                        <  1:420436/143‑1 (MQ=255)
                                             ggcccACGGGCGGCGAAAAAAACCCACCCCCCCGGCGGAGTCGAGAATAAACCGCAAATGAAGGCCGCGCCGCCCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCa                                                                                                            <  1:1537490/142‑1 (MQ=255)
                                              ggCCCCGGATTGGGGAAAAAAGCCAACTGCCGGGCGGAGTCGAGAATCAAGCGTTATTAATTGCGGCTTGGCGAGGCCGCCATTAATGATCCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAatat                                                                                                        <  2:448230/148‑1 (MQ=255)
                                              ggCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAAtattat                                                                                                     >  4:912791/1‑151 (MQ=255)
                                              ggCCACGGGCTGCGGAAAAAACCCACCCCCCCGGCGGAGTCGAGCATCAACCGGCAATGAATCCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAAtattat                                                                                                     <  2:128210/151‑1 (MQ=255)
                                              ggCCACGGGCTGCGAAAAAAAGCCACCCCCCCGGCGGAGTCGAGCATCAATCGGAAATGAAGGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAAtattat                                                                                                     <  2:51604/151‑1 (MQ=255)
                                                 cACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGgc                                                                                                  >  1:1355639/1‑151 (MQ=255)
                                                    gggcggagaaaaaaaCCCCCCCCCCCGGGGGTGGCGACCAAAAAGCGCCAATGATCCCCGCGCCCCGCCGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGgc                                                                                                  <  1:1563060/139‑1 (MQ=255)
                                                    gggCTGCGGAAAAAACCCCCCCCCCCGGCGGAGTCGACCATCAACCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGgcgc                                                                                                <  4:153994/150‑1 (MQ=255)
                                                    gggCTGCGAAAAAAACCCACCCCCCCGGCGGAGTCGAACATCAACCGGAAATGAAGCCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGgcg                                                                                                 <  4:1221939/149‑1 (MQ=255)
                                                     gggcggcgAAAAAAGCCCCCCCCCCGGGCGGAGCCGACCACAAACCGGCAATGAAGGCCGCGCCGCGCACCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGgcgc                                                                                                <  1:609559/143‑1 (MQ=255)
                                                     ggCTGAGAAAAAAACCCACCCCCCGGGCGGAGACGAACATAAAGCGGCAATAAATGCCGCGCCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGgcgcgc                                                                                              <  1:449906/151‑1 (MQ=255)
                                                      gCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGcgcgcg                                                                                             >  4:792001/1‑151 (MQ=255)
                                                        ggCGAAAAAAACCCACCCCCCGGGCGGAGTCGAGCATAAACCGGAAATAAAGGCCGCGCCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAg                                                                                           <  1:1539730/150‑1 (MQ=255)
                                                         gCGGAAAAAAGCCACCCCCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGa                                                                                            <  4:383194/149‑1 (MQ=255)
                                                          cGGAAAAAACCCTCACCCCGGGCGGATTAGAGAGCCAAAGGAAAATAAAGCCCGCCTCCCGCTGCCGCCATTAATGATTCAGCCTCCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCAGCTCAAtattat                                                                                                     <  3:76728/139‑1 (MQ=255)
                                                           ggAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTg                                                                                        >  1:454207/1‑151 (MQ=255)
                                                             aaaaaaGCCCCCCCCCCGGGGGAGTCGAGCATCAATCGGAAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGc                                                                                          <  1:622011/147‑1 (MQ=255)
                                                             aaaaaaGCCACCCCCCCGGCGGAGTCGAGCATCAATCGGCAATAAATCCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGa                                                                                      <  4:445223/151‑1 (MQ=255)
                                                                aaaGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGt                                                                                   >  4:427246/1‑151 (MQ=255)
                                                                          ccccccgggcggAGCCGAGCATCAATCGGCAATGAATGCCGCGGCGCGCAGCCGCCATTAATGATTCAGCCCCCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGa                                                                             <  1:1292865/143‑1 (MQ=255)
                                                                               ccggggggtccGAGCACAAATCGGCAATAAGGCCCGGGCCGCCCGGCCGCCATTAATGATTCAGCCCCCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACg                                                                       <  3:162998/141‑1 (MQ=255)
                                                                                    ggggTCGAGCATCAATCGGCAATGAATGCCGCGCCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTg                                                                   <  4:673466/147‑1 (MQ=255)
                                                                                    ggAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGc                                                                  >  4:1621731/1‑151 (MQ=255)
                                                                                    ggAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGc                                                                  >  4:930238/1‑151 (MQ=255)
                                                                                    ggAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGc                                                                  >  2:402058/1‑151 (MQ=255)
                                                                                       gTCGAGCATCAATCGGCAATGAATGCCGCGGCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGcatc                                                               >  1:476966/1‑151 (MQ=255)
                                                                                       gTCGAGCATAAACCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGcatc                                                               <  2:332482/151‑1 (MQ=255)
                                                                                          gaccATCAATCGGCAATAAAGCCCGCTGCGCGCAGCCGCCATTAATGATTCAGCCCCCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGcatca                                                              <  1:1706431/146‑1 (MQ=255)
                                                                                          gAGCATCAATCGGAAAGAAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGcatca                                                              <  4:1542139/149‑1 (MQ=255)
                                                                                                 aaccggcaagaaagcccgcgccgcgCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTg                                                     <  1:1774103/136‑1 (MQ=255)
                                                                                                 aaTCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTg                                                     >  2:149960/1‑151 (MQ=255)
                                                                                                 aaTCGGCAATGAAGGCCGCGCCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGcatcaca                                                            <  4:1927929/144‑1 (MQ=255)
                                                                                                    cGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACt                                                  >  1:1559580/1‑151 (MQ=255)
                                                                                                        aataaagcccGCGTGCCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCg                                                <  3:51478/141‑1 (MQ=255)
                                                                                                         atgaatgCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGtc                                             >  3:350625/1‑151 (MQ=255)
                                                                                                           gaatgCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGtcat                                           >  4:1426734/1‑151 (MQ=255)
                                                                                                             atcccgcgccgcgCACCCGCCATTAATGATTCAGCCCCCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGtcatca                                         <  2:356998/148‑1 (MQ=255)
                                                                                                              tgCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGtcatc                                          >  1:715517/1‑149 (MQ=255)
                                                                                                                ccgcgccgcgCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGtcatca                                         <  3:200786/148‑1 (MQ=255)
                                                                                                                  gcgTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGt                                    >  4:1778231/1‑151 (MQ=255)
                                                                                                                   cgTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGt                                    >  1:1219119/1‑150 (MQ=255)
                                                                                                                    gTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGt                                    >  4:512165/1‑149 (MQ=255)
                                                                                                                    gTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGt                                    <  2:215832/149‑1 (MQ=255)
                                                                                                                     tCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGc                                 >  4:407194/1‑151 (MQ=255)
                                                                                                                     tCGCGCAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGc                                 >  1:1375095/1‑151 (MQ=255)
                                                                                                                        cgcAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCt                                <  1:1109693/149‑1 (MQ=255)
                                                                                                                        cgcAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTg                               >  4:1720594/1‑150 (MQ=255)
                                                                                                                        cgcAGCCGCCATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTg                               >  4:1427761/1‑150 (MQ=255)
                                                                                                                          cggccgccATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCt                                <  1:417460/145‑1 (MQ=255)
                                                                                                                              cgccATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCt                        >  1:1052750/1‑151 (MQ=255)
                                                                                                                               gccATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCtt                       >  4:1534478/1‑151 (MQ=255)
                                                                                                                               gccATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCtt                       >  1:484610/1‑151 (MQ=255)
                                                                                                                               gccATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCtt                       >  4:321598/1‑151 (MQ=255)
                                                                                                                                 cATTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTg                     >  1:268799/1‑151 (MQ=255)
                                                                                                                                  aTTAATGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAACACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTg                     >  4:1469227/1‑150 (MQ=255)
                                                                                                                                      aTGATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGAcccc                >  4:1044700/1‑151 (MQ=255)
                                                                                                                                        gATTCAGCCCCCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCa               <  4:575395/150‑1 (MQ=255)
                                                                                                                                        gATTCAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGAcccc                >  1:1634953/1‑149 (MQ=255)
                                                                                                                                            cAGCCCCCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCg            <  1:791661/149‑1 (MQ=255)
                                                                                                                                            cAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGAt          >  2:70232/1‑151 (MQ=255)
                                                                                                                                            cAGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGAt          >  4:868816/1‑151 (MQ=255)
                                                                                                                                             aGCCACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATc         >  4:1835951/1‑151 (MQ=255)
                                                                                                                                               cccccTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGGCCCCAGCGATc         <  1:1629552/146‑1 (MQ=255)
                                                                                                                                               ccACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGAt          <  3:62529/148‑1 (MQ=255)
                                                                                                                                               ccACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATccc       <  1:362401/151‑1 (MQ=255)
                                                                                                                                               ccACCTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATcc        <  1:1560830/150‑1 (MQ=255)
                                                                                                                                                   cTGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTg    >  2:193836/1‑150 (MQ=255)
                                                                                                                                                    tGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTgac  >  1:833995/1‑151 (MQ=255)
                                                                                                                                                    tGGCCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTgac  >  1:1628290/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                   
CTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGAC  >  NC_000913/1308022‑1308317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: