BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,308,467	T→C	E60G (GAG→GGG)	clsA ←	cardiolipin synthase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,308,467	0	T	C	99.2%	461.2 / NA	130	E60G (GAG→GGG)	clsA	cardiolipin synthase 1
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (66/63); minor base G (0/1); total (66/64)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 130 total aligned reads displayed.

ACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCC  >  NC_000913/1308319‑1308613

acgCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCg                                                                                                                                                  <  4:484792/151‑1 (MQ=255)

acgCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCg                                                                                                                                                  <  3:152239/151‑1 (MQ=255)

   cTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGcc                                                                                                                                                >  2:17477/1‑150 (MQ=255)

    tCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCa                                                                                                                                               >  4:613358/1‑150 (MQ=255)

       cAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACgg                                                                                                                                           <  1:1011918/151‑1 (MQ=255)

        aaaGCTTGAATAATGGCGCAGCGAAACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACgg                                                                                                                                           >  4:456266/1‑150 (MQ=255)

            cTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCa                                                                                                                                         >  4:490536/1‑148 (MQ=255)

             ttGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCa                                                                                                                                         >  1:1693144/1‑147 (MQ=255)

              tGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGAt                                                                                                                                     >  1:1004179/1‑150 (MQ=255)

              tGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGAt                                                                                                                                     >  4:591128/1‑150 (MQ=255)

              tGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGAt                                                                                                                                     >  3:139444/1‑150 (MQ=255)

              tGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGAt                                                                                                                                     >  1:1052476/1‑150 (MQ=255)

              tGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGAt                                                                                                                                     <  1:1024625/150‑1 (MQ=255)

               gAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAg                                                                                                                                   <  3:210915/151‑1 (MQ=255)

                aataatGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAgg                                                                                                                                  >  4:1528947/1‑151 (MQ=255)

                   aatGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCa                                                                                                                                >  1:327942/1‑150 (MQ=255)

                     tGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCaat                                                                                                                              >  1:1168795/1‑150 (MQ=255)

                      ggCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCaata                                                                                                                             >  1:1314141/1‑150 (MQ=255)

                        cgcAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAAtt                                                                                                                          >  1:141230/1‑151 (MQ=255)

                        cgcAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAAtt                                                                                                                          >  1:1508348/1‑151 (MQ=255)

                         gcAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTc                                                                                                                         <  1:609597/151‑1 (MQ=255)

                           aGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCg                                                                                                                       <  1:1317304/151‑1 (MQ=255)

                               acacTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTa                                                                                                                   <  1:868443/151‑1 (MQ=255)

                                cacTGGTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCATATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCGCGCCAACGGCAAGATAGGCAATAATTCCGACTa                                                                                                                   <  3:160890/150‑1 (MQ=255)

                                 acTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGACACAACGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAAc                                                                                                                 >  4:1292448/1‑151 (MQ=255)

                                   tGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACgg                                                                                                               >  2:348300/1‑151 (MQ=255)

                                    gCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACgg                                                                                                               <  1:1533876/150‑1 (MQ=255)

                                     cTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGc                                                                                                              >  1:106101/1‑150 (MQ=255)

                                       aTTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGc                                                                                                              >  4:133776/1‑148 (MQ=255)

                                       aTTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGc                                                                                                              >  4:604346/1‑148 (MQ=255)

                                       aTTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCAGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCa                                                                                                             <  4:715831/149‑1 (MQ=255)

                                        ttttCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGa                                                                                                           >  1:426140/1‑150 (MQ=255)

                                         tttCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGaa                                                                                                          <  1:1835232/150‑1 (MQ=255)

                                         tttCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAAt                                                                                                         >  4:309377/1‑151 (MQ=255)

                                          ttcttcGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATg                                                                                                        >  4:1592186/1‑151 (MQ=255)

                                             ttcGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTa                                                                                                      >  1:1258214/1‑150 (MQ=255)

                                              tcGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTaa                                                                                                     <  4:268878/150‑1 (MQ=255)

                                              tcGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTaa                                                                                                     <  4:391403/150‑1 (MQ=255)

                                               cGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAaat                                                                                                   >  4:1274928/1‑151 (MQ=255)

                                               cGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAaat                                                                                                   >  1:1676608/1‑151 (MQ=255)

                                                  cGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAaataat                                                                                                >  3:367653/1‑151 (MQ=255)

                                                    aaGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCa                                                                                              <  3:542962/151‑1 (MQ=255)

                                                      gATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCACGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAAc                                                                                            >  4:1187329/1‑151 (MQ=255)

                                                       atatGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAAc                                                                                            >  4:1407326/1‑150 (MQ=255)

                                                       atatGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAAc                                                                                            >  4:889440/1‑150 (MQ=255)

                                                       atatGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACa                                                                                           <  1:1211807/151‑1 (MQ=255)

                                                          tGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGcc                                                                                        >  2:100927/1‑151 (MQ=255)

                                                                cAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCa                                                                                  <  1:1002175/151‑1 (MQ=255)

                                                                  ggCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATc                                                                                >  1:391965/1‑151 (MQ=255)

                                                                   gCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATcg                                                                               <  4:938576/151‑1 (MQ=255)

                                                                   gCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATcg                                                                               <  4:690857/151‑1 (MQ=255)

                                                                     tttAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATcgcg                                                                             >  1:1589454/1‑151 (MQ=255)

                                                                      ttAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATcgcg                                                                             <  4:1736502/150‑1 (MQ=255)

                                                                        aaGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCgg                                                                            >  2:79190/1‑149 (MQ=255)

                                                                           gTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAggg                                                                        >  4:1263727/1‑150 (MQ=255)

                                                                             cGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAAc                                                                     <  4:1557278/151‑1 (MQ=255)

                                                                             cGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAAc                                                                     <  1:788160/151‑1 (MQ=255)

                                                                                     cATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGcgtc                                                             >  1:505161/1‑151 (MQ=255)

                                                                                      aTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGcgtcg                                                            >  1:135121/1‑151 (MQ=255)

                                                                                          tGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGttt                                                         <  4:821694/150‑1 (MQ=255)

                                                                                           gCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTc                                                        <  4:1693811/150‑1 (MQ=255)

                                                                                             ggTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCAtt                                                     <  4:1588766/151‑1 (MQ=255)

                                                                                             ggTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCAtt                                                     <  1:1426157/151‑1 (MQ=255)

                                                                                               tGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCTTCGTTTc                                                        >  4:366694/1‑146 (MQ=255)

                                                                                                ggAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAg                                                   <  4:296476/150‑1 (MQ=255)

                                                                                                 gAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGa                                                  >  2:328338/1‑150 (MQ=255)

                                                                                                  aaGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAAt                                                >  4:1577954/1‑151 (MQ=255)

                                                                                                   aGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATg                                               <  1:1770516/151‑1 (MQ=255)

                                                                                                     gCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCg                                             <  1:1719925/151‑1 (MQ=255)

                                                                                                         caTCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTaa                                          >  1:98667/1‑150 (MQ=255)

                                                                                                          aTCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTaa                                          >  4:27358/1‑149 (MQ=255)

                                                                                                          aTCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTaa                                          >  4:1315448/1‑149 (MQ=255)

                                                                                                            cGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGt                                       <  4:1511468/150‑1 (MQ=255)

                                                                                                             gCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGtt                                      >  4:970837/1‑150 (MQ=255)

                                                                                                             gCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGtt                                      >  2:74590/1‑150 (MQ=255)

                                                                                                              ctctGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTAc                                    >  4:1355250/1‑151 (MQ=255)

                                                                                                                ctGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTAc                                    <  1:249846/149‑1 (MQ=255)

                                                                                                                ctGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGc                                  >  1:1184560/1‑151 (MQ=255)

                                                                                                                    cgcgcTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGc                              <  4:1369781/151‑1 (MQ=255)

                                                                                                                     gcgcTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCa                             <  1:1292365/151‑1 (MQ=255)

                                                                                                                     gcgcTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCa                             <  1:889053/151‑1 (MQ=255)

                                                                                                                      cgcTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCaa                            <  4:373071/151‑1 (MQ=255)

                                                                                                                      cgcTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCa                             <  3:394855/150‑1 (MQ=255)

                                                                                                                      cgcTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCa                             <  3:20908/150‑1 (MQ=255)

                                                                                                                       gcTCAGCGCGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAAt                           <  1:407052/151‑1 (MQ=255)

                                                                                                                            gcgcGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCa                      <  1:703625/151‑1 (MQ=255)

                                                                                                                            gcgcGGCGTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCa                      <  2:390812/151‑1 (MQ=255)

                                                                                                                              gcggcgTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAAc                    <  4:1270718/151‑1 (MQ=255)

                                                                                                                                 gcgTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAAcc                   >  4:1099518/1‑149 (MQ=255)

                                                                                                                                 gcgTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAAcc                   >  1:268189/1‑149 (MQ=255)

                                                                                                                                 gcgTTTGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAg                 <  4:1488768/151‑1 (MQ=255)

                                                                                                                                    tttGCCTAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTa               >  1:931058/1‑150 (MQ=255)

                                                                                                                                          tAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATccc           >  1:317038/1‑148 (MQ=255)

                                                                                                                                          tAAATGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATccc           >  1:1182602/1‑148 (MQ=255)

                                                                                                                                           aaaTGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAg         <  4:92074/149‑1 (MQ=255)

                                                                                                                                           aaaTGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGa        <  4:1308562/150‑1 (MQ=255)

                                                                                                                                           aaaTGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGa        <  1:211792/150‑1 (MQ=255)

                                                                                                                                            aaTGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAAt      <  4:1000592/151‑1 (MQ=255)

                                                                                                                                             aTGGAGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATg     <  4:1635594/151‑1 (MQ=255)

                                                                                                                                                 aGCCCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGcc  >  1:593550/1‑150 (MQ=255)

ACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCC  >  NC_000913/1308319‑1308613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`