Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,345,482 G→A Q421* (CAG→TAG)  gmr ← cyclic‑di‑GMP phosphodiesterase; csgD regulator; modulator of RNase II stability

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,345,4820GA100.0% 558.5 / NA 140Q421* (CAG→TAG) gmrcyclic‑di‑GMP phosphodiesterase; csgD regulator; modulator of RNase II stability
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (56/84);  total (56/84)

Only 100 of 140 total aligned reads displayed.

GGCACAATTAGCCCTGACTCTTCGGCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTGATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGC  >  NC_000913/1345334‑1345629
                                                                                                                                                    |                                                                                                                                                   
ggCACAATTAGCCCTGACTCTTCGGCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAAt                                                                                                                                                   >  4:1367747/1‑151 (MQ=255)
    caATTAGCCCTGACTCTTCGGCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGtt                                                                                                                                               <  1:535282/151‑1 (MQ=255)
      aTTAGCCCTGACTCTTCGGCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGtttt                                                                                                                                             >  4:1740857/1‑151 (MQ=255)
      aTTAGCCCTGACTCTTCGGCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGtttt                                                                                                                                             <  4:1690889/151‑1 (MQ=255)
      aTTAGCCCTGACTCTTCGGCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGCGATTTTCGGTTGATAGTGAATAACCAACTAATCGttt                                                                                                                                              >  4:461475/1‑150 (MQ=255)
        tAGCCCTGACTCTTCGGCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTc                                                                                                                                            <  1:1707134/150‑1 (MQ=255)
         aGCCCTGACTCTTCGGCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTcc                                                                                                                                           <  1:957891/150‑1 (MQ=255)
         aGCCCTGACTCTTCGGCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCa                                                                                                                                          <  4:921098/151‑1 (MQ=255)
         aGCCCTGACTCTTCGGCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCa                                                                                                                                          <  2:313594/151‑1 (MQ=255)
               gACTCTTCGGCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCtt                                                                                                                                    >  1:1127788/1‑151 (MQ=255)
               gACTCTTCGGCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCtt                                                                                                                                    <  1:119999/151‑1 (MQ=255)
               gACTCTTCGGCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCt                                                                                                                                     <  4:546886/150‑1 (MQ=255)
                 ctctTCGGCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCttt                                                                                                                                   >  2:248809/1‑150 (MQ=255)
                  tctTCGGCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTAc                                                                                                                                 >  3:166536/1‑151 (MQ=255)
                   ctTCGGCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTa                                                                                                                                  <  4:1264799/149‑1 (MQ=255)
                    ttCGGCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGc                                                                                                                               >  1:788695/1‑151 (MQ=255)
                     tCGGCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGGGAATAACCAACTAATCGTTTTCCAGTGCTTTACGc                                                                                                                               >  3:46767/1‑150 (MQ=255)
                      cGGCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCaa                                                                                                                             <  1:1532121/151‑1 (MQ=255)
                      cGGCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCa                                                                                                                              <  4:917512/150‑1 (MQ=255)
                       ggCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCa                                                                                                                              >  1:1631994/1‑149 (MQ=255)
                       ggCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAg                                                                                                                            <  1:597477/151‑1 (MQ=255)
                       ggCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAg                                                                                                                            <  4:340280/151‑1 (MQ=255)
                         cGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGtt                                                                                                                          <  3:540042/151‑1 (MQ=255)
                          gTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTg                                                                                                                         >  2:408345/1‑151 (MQ=255)
                            aGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTgg                                                                                                                        <  1:311360/150‑1 (MQ=255)
                            aGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGt                                                                                                                       <  3:411178/151‑1 (MQ=255)
                             ggAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTa                                                                                                                      <  4:1418709/151‑1 (MQ=255)
                               aaaTGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATc                                                                                                                    <  1:1356958/151‑1 (MQ=255)
                                aaTGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATcc                                                                                                                   <  2:401196/151‑1 (MQ=255)
                                aaTGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATcc                                                                                                                   <  1:703986/151‑1 (MQ=255)
                                 aTGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATcca                                                                                                                  <  1:384226/151‑1 (MQ=255)
                                 aTGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATcc                                                                                                                   >  1:1747292/1‑150 (MQ=255)
                                  tGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATcc                                                                                                                   >  4:189150/1‑149 (MQ=255)
                                      gTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATccagccag                                                                                                             <  4:1691171/151‑1 (MQ=255)
                                        ccAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCagag                                                                                                           <  3:218773/151‑1 (MQ=255)
                                        ccAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCagag                                                                                                           <  1:1058750/151‑1 (MQ=255)
                                        ccAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCaga                                                                                                            <  1:1438399/150‑1 (MQ=255)
                                              gTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTc                                                                                                     <  3:457827/151‑1 (MQ=255)
                                                ggAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTct                                                                                                    <  4:267686/150‑2 (MQ=255)
                                                ggAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCaa                                                                                                   <  1:15029/151‑1 (MQ=255)
                                                  aaTCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAAt                                                                                                 <  1:106647/151‑1 (MQ=255)
                                                      aaCCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATAccc                                                                                             >  3:140369/1‑151 (MQ=255)
                                                       aCCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCg                                                                                            >  4:1697752/1‑151 (MQ=255)
                                                         ccACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGtt                                                                                          >  4:1091771/1‑151 (MQ=255)
                                                               tCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTc                                                                                     >  1:1378655/1‑150 (MQ=255)
                                                               tCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTc                                                                                     <  1:551866/150‑1 (MQ=255)
                                                                cAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCa                                                                                    <  2:492778/150‑1 (MQ=255)
                                                                  ggTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCAttt                                                                                 <  4:11428/151‑1 (MQ=255)
                                                                     gACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTg                                                                              <  4:1716710/151‑1 (MQ=255)
                                                                         gCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTggg                                                                            <  4:1519114/149‑1 (MQ=255)
                                                                         gCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGt                                                                          <  3:408175/151‑1 (MQ=255)
                                                                           cAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTa                                                                         >  4:1808154/1‑150 (MQ=255)
                                                                            aaCGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTaaa                                                                       <  4:1128124/151‑1 (MQ=255)
                                                                            aaCGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTaa                                                                        >  4:1584936/1‑150 (MQ=255)
                                                                              cGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTaaaaa                                                                     <  1:295455/151‑1 (MQ=255)
                                                                                 aCTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACg                                                                   <  3:203296/150‑1 (MQ=255)
                                                                                 aCTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACg                                                                   <  1:1710393/150‑1 (MQ=255)
                                                                                 aCTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACg                                                                   <  4:1509960/150‑1 (MQ=255)
                                                                                 aCTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACg                                                                   <  1:1227370/150‑1 (MQ=255)
                                                                                 aCTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGc                                                                  >  4:1448162/1‑151 (MQ=255)
                                                                                   tAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCa                                                                 >  2:292553/1‑150 (MQ=255)
                                                                                    aGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCaa                                                                <  1:782828/150‑1 (MQ=255)
                                                                                    aGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCaa                                                                <  4:721871/150‑1 (MQ=255)
                                                                                     gTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCaaa                                                               >  4:1023342/1‑150 (MQ=255)
                                                                                      tGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAAt                                                             >  1:1411928/1‑151 (MQ=255)
                                                                                         ttCCAGACTGCGCACTTCGCCACGCCAGGTGATTCTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGt                                                          >  1:1256129/1‑151 (MQ=255)
                                                                                           ccAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTc                                                         <  2:150399/150‑1 (MQ=255)
                                                                                           ccAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTc                                                         <  4:71721/150‑1 (MQ=255)
                                                                                            cAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCt                                                       <  1:1343593/151‑1 (MQ=255)
                                                                                                 tGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTcc                                                  >  4:1125050/1‑151 (MQ=255)
                                                                                                  gcgcACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTc                                                   <  4:797539/149‑1 (MQ=255)
                                                                                                     cACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGcct                                              >  1:103206/1‑151 (MQ=255)
                                                                                                     cACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGcct                                              >  4:1919405/1‑151 (MQ=255)
                                                                                                         tCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGccttcc                                           <  4:1839855/150‑1 (MQ=255)
                                                                                                         tCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGccttcc                                           <  1:1785772/150‑1 (MQ=255)
                                                                                                          cgccacgccaGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGccttcctt                                         <  1:1477245/151‑1 (MQ=255)
                                                                                                          cgccacgccaGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGccttcctt                                         <  4:361659/151‑1 (MQ=255)
                                                                                                           gccacgccaGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCcttccttc                                        <  3:436938/151‑1 (MQ=255)
                                                                                                            ccacgccaGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCg                                       <  4:1484136/151‑1 (MQ=255)
                                                                                                              acgccaGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCt                                     <  1:206873/151‑1 (MQ=255)
                                                                                                              acgccaGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCt                                     >  4:153409/1‑151 (MQ=255)
                                                                                                                  caGGTGATTTTCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTgtgt                                 >  1:813526/1‑151 (MQ=255)
                                                                                                                           ttCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCtg                        >  4:1336227/1‑151 (MQ=255)
                                                                                                                           ttCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCtg                        >  4:1345407/1‑151 (MQ=255)
                                                                                                                            tCGGTTGATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCtgt                       >  4:1623129/1‑151 (MQ=255)
                                                                                                                                 tGATAGTGAATAACCAACTAATCGTTTTCCAGGGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCTCTGTGTACATTGCTGCGTCgg                  >  3:27776/1‑151 (MQ=255)
                                                                                                                                  gATAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCgg                  >  1:1103295/1‑150 (MQ=255)
                                                                                                                                    tAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGt               >  2:63220/1‑151 (MQ=255)
                                                                                                                                    tAGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGt               >  1:1307178/1‑151 (MQ=255)
                                                                                                                                     aGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGt               <  1:1697760/150‑1 (MQ=255)
                                                                                                                                     aGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTg              >  4:151483/1‑151 (MQ=255)
                                                                                                                                     aGTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTg              <  1:1080290/151‑1 (MQ=255)
                                                                                                                                      gTGAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTg              >  4:1612315/1‑150 (MQ=255)
                                                                                                                                        gAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACg           >  2:338490/1‑151 (MQ=255)
                                                                                                                                        gAATAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACg           <  1:1624149/151‑1 (MQ=255)
                                                                                                                                         aaTAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGAca           <  1:435621/150‑2 (MQ=255)
                                                                                                                                           tAACCAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGaat        >  2:56542/1‑151 (MQ=255)
                                                                                                                                               cAACTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGaataat     >  4:1720574/1‑150 (MQ=255)
                                                                                                                                                aaCTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGaataat     >  4:1491778/1‑149 (MQ=255)
                                                                                                                                                 aCTAATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGc  <  4:1328879/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGCACAATTAGCCCTGACTCTTCGGCGTAGGAAATGAAGTCCAACGGTGGAATCAACCCACGTTCAGGTGACTGCCAACGTACTAGTGCTTCCAGACTGCGCACTTCGCCACGCCAGGTGATTTTCGGTTGATAGTGAATAACCAACTGATCGTTTTCCAGTGCTTTACGCAAGTTGGTATCCAGCCAGAGATATTCAAATACCCGTTGATTCATTTCTGGGGTAAAAACGCAAAATTGTCCTCGTCCGCCTTCCTTCGCTGTGTACATTGCTGTGTCGGCGTGACGAATAATAGC  >  NC_000913/1345334‑1345629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑