Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,436,536 A→G Y120H (TAC→CAC)  ompN ← outer membrane pore protein N, non‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,436,5360AG100.0% 505.5 / NA 137Y120H (TAC→CAC) ompNouter membrane pore protein N, non‑specific
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (70/67);  total (70/67)

Only 100 of 137 total aligned reads displayed.

CGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTG  >  NC_000913/1436389‑1436681
                                                                                                                                                   |                                                                                                                                                 
cgAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCAt                                                                                                                                                >  2:271977/1‑151 (MQ=255)
cgAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCAt                                                                                                                                                >  4:1733987/1‑151 (MQ=255)
cgAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCAt                                                                                                                                                >  4:1777219/1‑151 (MQ=255)
 gAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCAtt                                                                                                                                               <  1:123583/151‑1 (MQ=255)
 gAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCAtt                                                                                                                                               >  1:28796/1‑151 (MQ=255)
 gAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCAtt                                                                                                                                               >  4:1844011/1‑151 (MQ=255)
        aGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCAt                                                                                                                                        <  4:228212/151‑1 (MQ=255)
        aGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCAt                                                                                                                                        >  1:1543012/1‑151 (MQ=255)
         gACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCAt                                                                                                                                        <  4:697856/150‑1 (MQ=255)
          aCCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATaa                                                                                                                                      <  2:150142/151‑1 (MQ=255)
           ccATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAAt                                                                                                                                     >  4:616657/1‑151 (MQ=255)
            cATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAAtt                                                                                                                                    <  4:295295/151‑1 (MQ=255)
            cATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAAtt                                                                                                                                    >  1:1421390/1‑151 (MQ=255)
               ttACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTAc                                                                                                                                  <  1:714687/150‑1 (MQ=255)
               ttACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACg                                                                                                                                 >  4:1664632/1‑151 (MQ=255)
                 aCCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGt                                                                                                                                <  1:476632/150‑1 (MQ=255)
                  ccAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTcc                                                                                                                              <  4:200849/151‑1 (MQ=255)
                   cAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCg                                                                                                                             >  1:1890037/1‑151 (MQ=255)
                    aGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGt                                                                                                                            >  4:1432787/1‑151 (MQ=255)
                     gACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTa                                                                                                                           <  1:1109699/151‑1 (MQ=255)
                     gACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTa                                                                                                                           <  2:252633/151‑1 (MQ=255)
                       ccGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAAt                                                                                                                         <  4:1501906/151‑1 (MQ=255)
                         gaagaaATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATc                                                                                                                        >  1:1587541/1‑150 (MQ=255)
                          aagaaATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGa                                                                                                                      <  4:1481109/151‑1 (MQ=255)
                          aagaaATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACCCCATAATTACGTCCGTAATCg                                                                                                                       >  1:1107910/1‑150 (MQ=255)
                            gaaATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGaa                                                                                                                     >  4:1257235/1‑150 (MQ=255)
                            gaaATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGaa                                                                                                                     >  1:427618/1‑150 (MQ=255)
                             aaaTCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGaa                                                                                                                     >  4:846403/1‑149 (MQ=255)
                              aaTCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGaa                                                                                                                     >  3:46574/1‑148 (MQ=255)
                                tCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGaa                                                                                                                 >  1:201804/1‑150 (MQ=255)
                                   gTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGt                                                                                                             <  1:1329135/151‑1 (MQ=255)
                                    tATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGt                                                                                                             >  4:531547/1‑150 (MQ=255)
                                     aTTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTaa                                                                                                           <  4:1690541/151‑1 (MQ=255)
                                     aTTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTa                                                                                                            <  1:1632568/150‑1 (MQ=255)
                                       tACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATc                                                                                                         >  4:311967/1‑151 (MQ=255)
                                        aCGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATc                                                                                                         <  4:426673/150‑1 (MQ=255)
                                        aCGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCt                                                                                                        <  1:509654/151‑1 (MQ=255)
                                         cGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTg                                                                                                       <  3:506825/151‑1 (MQ=255)
                                          gATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGc                                                                                                      <  1:1924422/151‑1 (MQ=255)
                                           aTAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCa                                                                                                     <  1:1224771/151‑1 (MQ=255)
                                                tCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAAttt                                                                                                >  4:458086/1‑151 (MQ=255)
                                                 cgcgACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAAttt                                                                                                <  1:738719/150‑1 (MQ=255)
                                                    gACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGc                                                                                            <  1:1701473/151‑1 (MQ=255)
                                                    gACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGc                                                                                            >  2:206225/1‑151 (MQ=255)
                                                     aCGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGc                                                                                            <  2:120442/150‑1 (MQ=255)
                                                        ccATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCgg                                                                                        <  2:228516/151‑1 (MQ=255)
                                                         cATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGc                                                                                       >  1:700314/1‑151 (MQ=255)
                                                             ggCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAAt                                                                                   <  2:205092/151‑1 (MQ=255)
                                                              gCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATg                                                                                  >  4:1026521/1‑151 (MQ=255)
                                                              gCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATg                                                                                  <  2:505008/151‑1 (MQ=255)
                                                                 cGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGcc                                                                                <  4:918093/150‑1 (MQ=255)
                                                                   aCCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGcc                                                                                >  3:262828/1‑148 (MQ=255)
                                                                   aCCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGa                                                                             <  4:687281/151‑1 (MQ=255)
                                                                     cAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACg                                                                           >  1:1112218/1‑151 (MQ=255)
                                                                      aGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACgg                                                                          <  3:352516/151‑1 (MQ=255)
                                                                      aGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACgg                                                                          >  1:1844921/1‑151 (MQ=255)
                                                                         cATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGt                                                                        >  1:1152950/1‑150 (MQ=255)
                                                                          aTAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTcc                                                                      >  1:472826/1‑151 (MQ=255)
                                                                            aaaGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCa                                                                     <  1:1377849/150‑1 (MQ=255)
                                                                            aaaGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCAt                                                                    >  4:783300/1‑151 (MQ=255)
                                                                                 tGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTTCATGACt                                                                <  3:537753/150‑1 (MQ=255)
                                                                                       cATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGttttt                                                         <  4:1593074/151‑1 (MQ=255)
                                                                                       cATTGCGATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGttttt                                                         >  1:88345/1‑151 (MQ=255)
                                                                                           ggTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGa                                                      >  4:1750667/1‑150 (MQ=255)
                                                                                            gTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGa                                                      >  1:705065/1‑149 (MQ=255)
                                                                                                aaGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTc                                                <  4:858691/151‑1 (MQ=255)
                                                                                                  gagTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAg                                              <  2:166585/151‑1 (MQ=255)
                                                                                                   agTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAgt                                             <  4:433669/151‑1 (MQ=255)
                                                                                                      cACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGtgtt                                          <  1:891914/151‑1 (MQ=255)
                                                                                                      cACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGtgtt                                          >  1:1833680/1‑151 (MQ=255)
                                                                                                      cACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGtgtt                                          <  4:1351547/151‑1 (MQ=255)
                                                                                                      cACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGtgtt                                          >  4:1039254/1‑151 (MQ=255)
                                                                                                      cACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGtgtt                                          <  4:1563839/151‑1 (MQ=255)
                                                                                                         cGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTgttgtt                                       >  4:1535557/1‑151 (MQ=255)
                                                                                                          gCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGttt                                      <  1:60534/151‑1 (MQ=255)
                                                                                                           ccAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTg                                     >  4:1837809/1‑151 (MQ=255)
                                                                                                           ccAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTg                                     >  4:914264/1‑151 (MQ=255)
                                                                                                           ccAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTg                                     <  1:947294/151‑1 (MQ=255)
                                                                                                               aTTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTg                                 <  3:276600/151‑1 (MQ=255)
                                                                                                                ttCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTg                                 >  1:1210905/1‑150 (MQ=255)
                                                                                                                  cAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAat                              <  1:81290/151‑1 (MQ=255)
                                                                                                                     gcagcaTATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATAtt                           <  3:313021/151‑1 (MQ=255)
                                                                                                                     gcagcaTATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATAtt                           >  4:1202306/1‑151 (MQ=255)
                                                                                                                      cagcaTATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTg                          <  3:398313/151‑1 (MQ=255)
                                                                                                                          atatCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTAtt                      >  1:783226/1‑151 (MQ=255)
                                                                                                                          atatCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTAtt                      >  4:329670/1‑151 (MQ=255)
                                                                                                                            atCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTc                     <  1:116248/150‑1 (MQ=255)
                                                                                                                               ggTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCAt                 >  4:1026551/1‑151 (MQ=255)
                                                                                                                                  ccAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTg               <  4:1166054/150‑1 (MQ=255)
                                                                                                                                  ccAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTg               <  1:1241951/150‑1 (MQ=255)
                                                                                                                                   cAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAc             <  1:1892460/151‑1 (MQ=255)
                                                                                                                                   cAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAc             >  4:766829/1‑151 (MQ=255)
                                                                                                                                    aGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAcc            >  1:310601/1‑151 (MQ=255)
                                                                                                                                     gCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCg           <  1:1663977/151‑1 (MQ=255)
                                                                                                                                        ttCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGt          >  4:1003647/1‑149 (MQ=255)
                                                                                                                                           gATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCa     <  4:1336652/151‑1 (MQ=255)
                                                                                                                                              gTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGt   <  1:1843292/150‑1 (MQ=255)
                                                                                                                                              gTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGt   <  1:311866/150‑1 (MQ=255)
                                                                                                                                               tCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTg  >  3:89562/1‑150 (MQ=255)
                                                                                                                                               tCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTg  >  4:1870926/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTG  >  NC_000913/1436389‑1436681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑