Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,883,282 T→C L32P (CTC→CCC)  yeaV → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,883,2820TC99.3% 489.3 / ‑4.8 140L32P (CTC→CCC) yeaVputative transporter
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (75/63);  total (77/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 144 total aligned reads displayed.

TTATGTAACATGCAAATTTTGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCTCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAGGCACTCGAATTTAGCGTTCCCTACT  >  NC_000913/1883135‑1883428
                                                                                                                                                   |                                                                                                                                                  
ttATGTAACATGCAAATTTTGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCcctc                                                                                                                                                 >  4:465039/1‑151 (MQ=255)
  aTGTAACATGCAAATTTTGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCcctcct                                                                                                                                               <  1:899463/151‑1 (MQ=255)
  aTGTAACATGCAAATTTTGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCcctcct                                                                                                                                               <  1:1482853/151‑1 (MQ=255)
  aTGTAACATGCAAATTTTGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCcctcct                                                                                                                                               <  4:1337118/151‑1 (MQ=255)
   tGTAACATGCAAATTTTGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTg                                                                                                                                              >  4:1185420/1‑151 (MQ=255)
      aaCATGCAAATTTTGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCt                                                                                                                                           >  1:517893/1‑151 (MQ=255)
       aCATGCAAATTTTGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCtg                                                                                                                                          >  3:424367/1‑151 (MQ=255)
         aTGCAAATTTTGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCtgtt                                                                                                                                        <  3:394446/151‑1 (MQ=255)
           gCAAATTTTGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCtgttt                                                                                                                                       <  1:1263305/150‑1 (MQ=255)
            cAAATTTTGTTACGCGAACGCTAGGTTCCGCCGTACAGGTATTGGTTTTCCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCtgtttat                                                                                                                                     >  1:1919027/1‑151 (MQ=255)
             aaaTTTTGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCtgtttatg                                                                                                                                    >  4:729181/1‑151 (MQ=255)
               aTTTTGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCtgtttatgtt                                                                                                                                  <  1:1159131/151‑1 (MQ=255)
                  ttGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTgtttatgtttat                                                                                                                               >  4:571226/1‑151 (MQ=255)
                    gTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTAtt                                                                                                                              <  1:801909/150‑1 (MQ=255)
                    gTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTAtt                                                                                                                              <  1:520978/150‑1 (MQ=255)
                     ttACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTtg                                                                                                                            >  2:197366/1‑151 (MQ=255)
                       aCGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTtgtg                                                                                                                          >  1:1312996/1‑151 (MQ=255)
                       aCGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTtgtg                                                                                                                          <  1:1407777/151‑1 (MQ=255)
                       aCGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTtgtg                                                                                                                          <  2:406284/151‑1 (MQ=255)
                       aCGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTtgt                                                                                                                           <  4:1378973/150‑1 (MQ=255)
                         gcgTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGcc                                                                                                                        >  1:1012872/1‑151 (MQ=255)
                           gTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCgg                                                                                                                      <  4:289577/151‑1 (MQ=255)
                             aCGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGtt                                                                                                                    <  2:234887/151‑1 (MQ=255)
                             aCGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGtt                                                                                                                    >  1:1866707/1‑151 (MQ=255)
                              cGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGttt                                                                                                                   <  1:1511340/151‑1 (MQ=255)
                               gTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGttt                                                                                                                   >  1:888310/1‑150 (MQ=255)
                               gTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTa                                                                                                                  <  4:1757106/151‑1 (MQ=255)
                               gTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTa                                                                                                                  <  4:1447777/151‑1 (MQ=255)
                               gTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTa                                                                                                                  <  4:1186757/151‑1 (MQ=255)
                               gTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTa                                                                                                                  <  1:798038/151‑1 (MQ=255)
                                ttAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGttt                                                                                                                   >  4:778407/1‑149 (MQ=255)
                                    gTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGttc                                                                                                             >  1:1332775/1‑151 (MQ=255)
                                      tCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGttctt                                                                                                           >  3:436336/1‑151 (MQ=255)
                                       ccgccgTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGttcttc                                                                                                          >  2:58800/1‑151 (MQ=255)
                                         gccgTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTa                                                                                                        <  2:511132/151‑1 (MQ=255)
                                          ccgTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTAc                                                                                                       >  3:131427/1‑151 (MQ=255)
                                          ccgTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTAc                                                                                                       <  4:57171/151‑1 (MQ=255)
                                          ccgTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTAc                                                                                                       <  4:1745908/151‑1 (MQ=255)
                                              aCAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCt                                                                                                    <  1:815355/150‑1 (MQ=255)
                                                aGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTa                                                                                                 <  4:5660/151‑1 (MQ=255)
                                                   tATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTg                                                                                              >  4:1262433/1‑151 (MQ=255)
                                                    aTTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTgg                                                                                             <  1:1199183/151‑1 (MQ=255)
                                                       ggTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTgggg                                                                                           <  2:205602/150‑1 (MQ=255)
                                                           ttGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGtt                                                                                       <  1:55066/150‑1 (MQ=255)
                                                            tGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTg                                                                                      <  3:365485/150‑1 (MQ=255)
                                                            tGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGc                                                                                     >  1:1588972/1‑151 (MQ=255)
                                                                ggCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTg                                                                                   <  4:582231/149‑1 (MQ=255)
                                                                ggCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGaa                                                                                 <  1:568649/151‑1 (MQ=255)
                                                                ggCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGa                                                                                  <  1:1236902/150‑1 (MQ=255)
                                                                 gCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAAt                                                                                <  1:1568422/151‑1 (MQ=255)
                                                                  cAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATg                                                                               >  1:1440499/1‑151 (MQ=255)
                                                                  cAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATg                                                                               >  1:996963/1‑151 (MQ=255)
                                                                   aaTGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATgg                                                                              >  1:1004668/1‑151 (MQ=255)
                                                                    aTGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATggg                                                                             <  4:1234384/151‑1 (MQ=255)
                                                                     tGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGc                                                                            >  1:1833448/1‑151 (MQ=255)
                                                                       ggACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCt                                                                          <  4:1928887/151‑1 (MQ=255)
                                                                        gACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCta                                                                         <  4:67261/151‑1 (MQ=255)
                                                                         aCTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCtat                                                                        >  1:531237/1‑151 (MQ=255)
                                                                          cTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAGAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCtatt                                                                       >  2:291714/1‑151 (MQ=255)
                                                                          cTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCtatt                                                                       >  4:1842059/1‑151 (MQ=255)
                                                                              tGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCtattatt                                                                    >  1:1395222/1‑150 (MQ=255)
                                                                              tGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCtattatt                                                                    >  1:1016859/1‑150 (MQ=255)
                                                                                    atttaGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCaaa                                                              <  1:1796061/150‑1 (MQ=255)
                                                                                    atttaGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCaaa                                                              <  4:561238/150‑1 (MQ=255)
                                                                                    atttaGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCaaa                                                              <  4:1899339/150‑1 (MQ=255)
                                                                                      ttaGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAaca                                                            <  1:1776216/150‑1 (MQ=255)
                                                                                           caccaGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTgg                                                       >  4:1873156/1‑150 (MQ=255)
                                                                                               ggTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTa                                                   >  4:37150/2‑150 (MQ=255)
                                                                                                gTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTaaa                                                 <  4:1753572/151‑1 (MQ=255)
                                                                                                 tAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTaaa                                                 <  3:495335/150‑1 (MQ=255)
                                                                                                 tAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAat                                                >  1:11140/1‑151 (MQ=255)
                                                                                                        ggCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCAc                                         >  4:415052/1‑151 (MQ=255)
                                                                                                         gCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCAcc                                        >  2:98709/1‑151 (MQ=255)
                                                                                                          cAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACcg                                       <  1:1623194/151‑1 (MQ=255)
                                                                                                           aaTATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACcgc                                      >  4:67025/1‑151 (MQ=255)
                                                                                                            atatTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACcgcg                                     >  4:495204/1‑151 (MQ=255)
                                                                                                              atTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCAGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGt                                    <  2:155029/150‑1 (MQ=255)
                                                                                                                 cGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTca                                 <  4:1658935/150‑1 (MQ=255)
                                                                                                                  gTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTcaca                               <  1:828618/151‑1 (MQ=255)
                                                                                                                  gTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTcac                                >  4:185006/1‑150 (MQ=255)
                                                                                                                    cTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCacaac                             >  3:466385/1‑151 (MQ=255)
                                                                                                                    cTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCacaac                             >  1:1212216/1‑151 (MQ=255)
                                                                                                                     ttGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCacaac                             >  1:817185/1‑150 (MQ=255)
                                                                                                                       ggCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAgg                          <  1:1301492/151‑1 (MQ=255)
                                                                                                                       ggCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAg                           <  1:290481/150‑1 (MQ=255)
                                                                                                                         cGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAGGc                         >  1:2511/1‑150 (MQ=255)
                                                                                                                         cGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAGGCa                        <  4:1060430/151‑1 (MQ=255)
                                                                                                                         cGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAGGCa                        <  4:446137/151‑1 (MQ=255)
                                                                                                                          gAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAGGCAc                       <  2:279805/151‑1 (MQ=255)
                                                                                                                              gAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAGGCACTCg                    >  1:324214/1‑150 (MQ=255)
                                                                                                                               aaaaCCGGAATACAGCACGCTCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAGGCACTCGa                   >  4:1061615/1‑150 (MQ=255)
                                                                                                                                aaaCCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAGGCACTCGAAt                 >  1:1654663/1‑151 (MQ=255)
                                                                                                                                   ccGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAGGCACTCGAATTTa              <  2:442437/151‑1 (MQ=255)
                                                                                                                                    cGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAGGCACTCGAAttt               >  3:501633/1‑149 (MQ=255)
                                                                                                                                      gAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAGGCACTCGAATTTAGCg           >  1:752814/1‑151 (MQ=255)
                                                                                                                                       aaTACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAGGCACTCGAATTTAGCGt          <  1:67007/151‑1 (MQ=255)
                                                                                                                                          aCAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAGGCACTCGAATTTAGCGTTc        <  1:1251783/150‑1 (MQ=255)
                                                                                                                                          aCAGCACGCACTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGATCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCATCTTATTATCAAAAACCTGGATTAAATATCGCACCGCGTTCACAACAGGCACTCGAATTTAGCGTTcc       >  1:1707674/1‑151 (MQ=255)
                                                                                                                                           cAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAGGCACTCGAATTTAGCGTTccc      <  1:251979/151‑1 (MQ=255)
                                                                                                                                               aCGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAGGCACTCGAATTTAGCGTTCCCTAct  >  3:151163/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTATGTAACATGCAAATTTTGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCTCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAGGCACTCGAATTTAGCGTTCCCTACT  >  NC_000913/1883135‑1883428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑