BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,049,101	T→C	G1388G (GGT→GGC)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,049,101	0	T	C	99.3%	490.7 / NA	136	G1388G (GGT→GGC)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (64/71); minor base G (1/0); total (65/71)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

ATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACC  >  NC_000913/2048953‑2049248

aTGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGgcgt                                                                                                                                                   <  2:232630/151‑1 (MQ=255)

aTGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGgcgt                                                                                                                                                   >  4:703282/1‑151 (MQ=255)

     aGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTccccc                                                                                                                                              >  1:1843616/1‑151 (MQ=255)

      gCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCca                                                                                                                                             <  1:369599/151‑1 (MQ=255)

       cTCCACGCTTAGCACATCAATTAATGTCAACGCTAATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTccccc                                                                                                                                              >  1:1868988/1‑149 (MQ=255)

         ccACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCcagca                                                                                                                                          <  3:416311/151‑1 (MQ=255)

          cACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCcagcag                                                                                                                                         <  3:23982/151‑1 (MQ=255)

          cACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCcagcag                                                                                                                                         <  4:1228561/151‑1 (MQ=255)

               ttAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCaggagg                                                                                                                                     >  1:1617965/1‑150 (MQ=255)

                tAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGt                                                                                                                                    >  1:1447945/1‑150 (MQ=255)

                 aGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTa                                                                                                                                   <  3:542276/150‑1 (MQ=255)

                    acaTCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAAccc                                                                                                                               >  4:11848/1‑151 (MQ=255)

                     caTCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCt                                                                                                                              >  1:1114516/1‑151 (MQ=255)

                         aaTTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGc                                                                                                                          >  4:504630/1‑151 (MQ=255)

                              aTGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTGTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGAAAAATGAGGAGAAGGATAACCACGGGAAAGGGGACCCCCAGCAGGAGGCAACCCTCAGCGttt                                                                                                                      >  4:1025536/1‑150 (MQ=255)

                                  cAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTATCCGCAGGCGAGACAACCAGTCTGTATATTGAGGAGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCAcc                                                                                                                  >  1:1445235/1‑150 (MQ=255)

                                   aaCGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCaa                                                                                                                <  4:359986/151‑1 (MQ=255)

                                     cGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGt                                                                                                              <  4:1569802/151‑1 (MQ=255)

                                      gCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTg                                                                                                             >  3:2725/1‑151 (MQ=255)

                                        tGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGa                                                                                                            >  4:304012/1‑150 (MQ=255)

                                          aTGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCCCCAAGTGAAgg                                                                                                         <  3:519567/151‑1 (MQ=255)

                                          aTGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAgg                                                                                                         >  4:1472992/1‑151 (MQ=255)

                                            gCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGc                                                                                                        <  1:1275862/150‑1 (MQ=255)

                                             cGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGt                                                                                                      <  1:1815462/151‑1 (MQ=255)

                                             cGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGt                                                                                                      >  4:151422/1‑151 (MQ=255)

                                              gTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTg                                                                                                     >  1:1227679/1‑151 (MQ=255)

                                                cTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGAc                                                                                                   >  4:955598/1‑151 (MQ=255)

                                                cTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGAc                                                                                                   >  1:943658/1‑151 (MQ=255)

                                                cTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGAc                                                                                                   >  4:52735/1‑151 (MQ=255)

                                                  aCGGCACATCTCACCTTGCTACAGGCACTTTTGGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCGCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGAccc                                                                                                 <  1:138349/151‑1 (MQ=255)

                                                   cGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGAcccc                                                                                                <  2:213468/151‑1 (MQ=255)

                                                     gCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCa                                                                                              <  1:1225615/151‑1 (MQ=255)

                                                         aTCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGta                                                                                           >  1:1597626/1‑150 (MQ=255)

                                                             cACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGtaataa                                                                                       <  1:506191/150‑1 (MQ=255)

                                                             cACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGtaat                                                                                         <  4:268203/148‑1 (MQ=255)

                                                              aCCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACg                                                                                     >  3:230001/1‑151 (MQ=255)

                                                              aCCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACg                                                                                     <  3:74686/151‑1 (MQ=255)

                                                                   gCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCtata                                                                                <  4:1796709/151‑1 (MQ=255)

                                                                    cTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCtatat                                                                               >  4:1283781/1‑151 (MQ=255)

                                                                      aCAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCtatatat                                                                             <  2:204782/151‑1 (MQ=255)

                                                                       cAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCtatatata                                                                            <  4:1015787/151‑1 (MQ=255)

                                                                       cAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCtatatat                                                                             >  4:1912204/1‑150 (MQ=255)

                                                                           cACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATAtactac                                                                        >  4:1383098/1‑151 (MQ=255)

                                                                             cTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGTCCCCCAGTAATAACGCTATATATACTacc                                                                       >  4:394506/1‑150 (MQ=255)

                                                                               ttttGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTaccaac                                                                    >  1:645816/1‑151 (MQ=255)

                                                                               ttttGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTaccaa                                                                     <  1:571347/150‑1 (MQ=255)

                                                                               ttttGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTacca                                                                      <  1:680662/149‑1 (MQ=255)

                                                                                tttGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTaccaac                                                                    <  4:1122804/150‑1 (MQ=255)

                                                                                tttGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTaccaac                                                                    <  3:223649/150‑1 (MQ=255)

                                                                                 ttGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTaccaacc                                                                   <  1:175577/150‑1 (MQ=255)

                                                                                  tGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTaccaacca                                                                  <  4:484817/150‑1 (MQ=255)

                                                                                  tGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTaccaacca                                                                  <  4:1838081/150‑1 (MQ=255)

                                                                                   gATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCac                                                                 <  4:841644/150‑1 (MQ=255)

                                                                                    aTACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCacga                                                               <  4:1342430/151‑1 (MQ=255)

                                                                                    aTACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCacg                                                                >  2:53126/1‑150 (MQ=255)

                                                                                      aCAGTCTCCGCAGGCGAGACAACCCGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCCCCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCacgac                                                              <  3:178472/150‑1 (MQ=255)

                                                                                       cAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACgg                                                            <  4:311090/151‑1 (MQ=255)

                                                                                         gTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCa                                                          <  3:52285/151‑1 (MQ=255)

                                                                                             ccGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAAttt                                                      <  2:537697/151‑1 (MQ=255)

                                                                                             ccGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCATCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAAt                                                        <  1:252406/149‑1 (MQ=255)

                                                                                              cGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAAtttt                                                     >  4:595774/1‑151 (MQ=255)

                                                                                                cAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTa                                                   <  1:428717/151‑1 (MQ=255)

                                                                                                 aGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTAc                                                  >  2:72367/1‑151 (MQ=255)

                                                                                                    cGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTAc                                                  <  1:823999/148‑1 (MQ=255)

                                                                                                      agaCAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCa                                               >  1:436370/1‑149 (MQ=255)

                                                                                                      agaCAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCa                                               >  1:1888131/1‑149 (MQ=255)

                                                                                                       gaCAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGc                                            <  2:384957/151‑1 (MQ=255)

                                                                                                         cAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCtt                                          >  1:702794/1‑151 (MQ=255)

                                                                                                          aaCCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCttt                                         >  2:394539/1‑151 (MQ=255)

                                                                                                          aaCCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCttt                                         >  2:109576/1‑151 (MQ=255)

                                                                                                            ccAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTAc                                       <  2:452698/151‑1 (MQ=255)

                                                                                                             cAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTAcc                                      >  2:107997/1‑151 (MQ=255)

                                                                                                                   gTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCt                                   <  2:163294/148‑1 (MQ=255)

                                                                                                                   gTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTAc                                 <  4:892248/150‑1 (MQ=255)

                                                                                                                    tataTTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACa                                >  2:306147/1‑150 (MQ=255)

                                                                                                                      tatTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACaaa                              >  1:1631177/1‑150 (MQ=255)

                                                                                                                      tatTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACaaa                              >  1:1061950/1‑150 (MQ=255)

                                                                                                                          gAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCg                         <  4:465946/151‑1 (MQ=255)

                                                                                                                           aGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCgg                        >  1:1043922/1‑151 (MQ=255)

                                                                                                                           aGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCgg                        >  1:824672/1‑151 (MQ=255)

                                                                                                                           aGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCgg                        <  4:1888860/151‑1 (MQ=255)

                                                                                                                            ggTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCggg                       <  4:1604070/151‑1 (MQ=255)

                                                                                                                             gTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCgggg                      <  2:442723/151‑1 (MQ=255)

                                                                                                                                aaGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGttt                   >  1:1034702/1‑151 (MQ=255)

                                                                                                                                 aGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTa                  <  1:82378/151‑1 (MQ=255)

                                                                                                                                   gATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATc                >  4:511944/1‑151 (MQ=255)

                                                                                                                                    aTAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCa               <  2:153397/151‑1 (MQ=255)

                                                                                                                                     tAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCa               <  4:833942/150‑1 (MQ=255)

                                                                                                                                      aaTTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAAt             >  3:407392/1‑151 (MQ=255)

                                                                                                                                      aaTTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAAt             <  4:255504/151‑1 (MQ=255)

                                                                                                                                       aTTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAAtt            <  2:239588/151‑1 (MQ=255)

                                                                                                                                       aTTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAAtt            <  4:1179790/151‑1 (MQ=255)

                                                                                                                                         tACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAATTg           >  4:1169834/1‑150 (MQ=255)

                                                                                                                                         tACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAATTg           >  4:1911177/1‑150 (MQ=255)

                                                                                                                                          aCGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAATTGa          <  4:837239/150‑1 (MQ=255)

                                                                                                                                          aCGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAATTGAc         <  4:1432900/151‑1 (MQ=255)

                                                                                                                                            ggCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAATTGACgg       >  3:192636/1‑151 (MQ=255)

                                                                                                                                                aCGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAAc   >  2:22234/1‑151 (MQ=255)

                                                                                                                                                aCGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAAc   >  1:1419192/1‑151 (MQ=255)

                                                                                                                                                 cGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAAcc  >  1:342529/1‑151 (MQ=255)

ATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACC  >  NC_000913/2048953‑2049248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`