Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 246,784 G→A A25T (GCC→ACC)  yafL → putative lipoprotein and C40 family peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913246,7840GA98.1% 542.4 / NA 156A25T (GCC→ACC) yafLputative lipoprotein and C40 family peptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (88/65);  minor base C (0/3);  total (88/68)
Fisher's exact test for biased strand distribution p-value = 8.08e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGC  >  NC_000913/246636‑246929
                                                                                                                                                    |                                                                                                                                                 
taatGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTAcc                                                                                                                                                 >  1:1008891/1‑151 (MQ=255)
taatGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTAcc                                                                                                                                                 >  4:846069/1‑151 (MQ=255)
 aatGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCa                                                                                                                                                <  4:1111940/151‑1 (MQ=255)
    ggTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAgcg                                                                                                                                             >  3:252096/1‑151 (MQ=255)
    ggTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAgcg                                                                                                                                             >  4:1792489/1‑151 (MQ=255)
    ggTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAgc                                                                                                                                              <  4:57369/150‑1 (MQ=255)
         attaAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGccacc                                                                                                                                        >  4:193412/1‑151 (MQ=255)
         attaAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGccacc                                                                                                                                        >  1:1391348/1‑151 (MQ=255)
          ttaAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGccacc                                                                                                                                        >  1:254550/1‑150 (MQ=255)
            aaGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCcaccac                                                                                                                                      >  4:1157129/1‑150 (MQ=255)
                 tGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACcacatcac                                                                                                                                 >  4:1483975/1‑150 (MQ=255)
                   ttCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACcacatcacat                                                                                                                               <  3:437813/150‑1 (MQ=255)
                    tCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACatat                                                                                                                             >  2:14866/1‑151 (MQ=255)
                     cAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACatat                                                                                                                             <  1:1172045/150‑1 (MQ=255)
                     cAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATAtc                                                                                                                            <  4:299143/151‑1 (MQ=255)
                       gAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATAtctc                                                                                                                          >  4:1528448/1‑151 (MQ=255)
                          aCGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCttt                                                                                                                       <  4:1208523/151‑1 (MQ=255)
                           cGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTc                                                                                                                      <  3:250650/151‑1 (MQ=255)
                            gATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTc                                                                                                                      >  1:1909097/1‑150 (MQ=255)
                            gATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTc                                                                                                                      >  3:77674/1‑150 (MQ=255)
                               ccGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCt                                                                                                                  <  1:797250/151‑1 (MQ=255)
                               ccGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCt                                                                                                                  >  4:94491/1‑151 (MQ=255)
                                cGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCt                                                                                                                  >  4:1602102/1‑150 (MQ=255)
                                cGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCt                                                                                                                  >  4:1414624/1‑150 (MQ=255)
                                cGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCt                                                                                                                  >  4:1592280/1‑150 (MQ=255)
                                 gggATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTAc                                                                                                                >  1:966476/1‑151 (MQ=255)
                                 gggATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTAc                                                                                                                >  2:377199/1‑151 (MQ=255)
                                    aTACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTAcgcc                                                                                                             >  4:1525017/1‑151 (MQ=255)
                                       cacTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACgccgcc                                                                                                          >  1:1894252/1‑151 (MQ=255)
                                        acTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGccgccc                                                                                                         >  4:1087766/1‑151 (MQ=255)
                                        acTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGccgccc                                                                                                         <  1:1843149/151‑1 (MQ=255)
                                         cTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGccgcccg                                                                                                        >  4:1676831/1‑151 (MQ=255)
                                          tGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGccgcccgc                                                                                                       >  3:309003/1‑151 (MQ=255)
                                             aaCATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCcgcccgcc                                                                                                      >  4:1264039/1‑149 (MQ=255)
                                             aaCATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCa                                                                                                     <  1:1829366/150‑1 (MQ=255)
                                             aaCATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCa                                                                                                     <  1:1141246/150‑1 (MQ=255)
                                                  tAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGAt                                                                                               >  2:26436/1‑151 (MQ=255)
                                                   aaaGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGAt                                                                                               <  4:321745/150‑1 (MQ=255)
                                                   aaaGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATg                                                                                              >  1:528562/1‑151 (MQ=255)
                                                       cttGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTTTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCaa                                                                                           <  1:1216549/148‑1 (MQ=255)
                                                       cATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCaa                                                                                           >  4:1298566/1‑150 (MQ=255)
                                                       cATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCaa                                                                                           >  2:122865/1‑150 (MQ=255)
                                                          gACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGaaa                                                                                           <  1:637702/147‑1 (MQ=255)
                                                          gACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAAcc                                                                                       >  4:967499/1‑151 (MQ=255)
                                                           aCCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAAcc                                                                                       <  4:782496/150‑1 (MQ=255)
                                                            ccAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGt                                                                                     >  1:708558/1‑151 (MQ=255)
                                                            ccAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGt                                                                                     >  4:1008992/1‑151 (MQ=255)
                                                             cAGCCATAATTGAGTTTGTCCTTGCCGTCGTTTCCATCCTTTGTATGGCCGGGATTACTGTTAATTTGTTTGCCGTTTTCTTCATTTCCCAGCGCCCCCACATCACATACCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCg                                                                                      <  4:70463/149‑1 (MQ=255)
                                                                ccATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTgcgc                                                                                 <  4:280673/151‑1 (MQ=255)
                                                                 cATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCCCCACATCCCATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTg                                                                                    <  4:709600/147‑1 (MQ=255)
                                                                 cATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGcgcg                                                                                <  1:1099686/151‑1 (MQ=255)
                                                                   ttattGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGt                                                                               <  1:1687299/150‑1 (MQ=255)
                                                                     attGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGttt                                                                             >  4:781832/1‑150 (MQ=255)
                                                                            aTGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGGTTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTaaaa                                                                      >  4:974757/1‑150 (MQ=255)
                                                                              gTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAAc                                                                     >  1:1812138/1‑149 (MQ=255)
                                                                                ccTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCCCCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAg                                                                   <  1:1264543/149‑1 (MQ=255)
                                                                                   tGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTAcc                                                               <  1:1529145/150‑1 (MQ=255)
                                                                                    gCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCa                                                              <  4:1077180/150‑1 (MQ=255)
                                                                                     ccGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCaa                                                             >  2:7026/1‑150 (MQ=255)
                                                                                     ccGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCAACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCaa                                                             <  1:1259424/150‑1 (MQ=255)
                                                                                       gTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACt                                                          <  4:649847/151‑1 (MQ=255)
                                                                                       gTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACt                                                          <  2:41463/151‑1 (MQ=255)
                                                                                        tCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTc                                                         >  1:20194/1‑151 (MQ=255)
                                                                                           aTTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATc                                                      >  1:7172/1‑151 (MQ=255)
                                                                                            ttCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCt                                                     >  1:1780902/1‑151 (MQ=255)
                                                                                               cATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGa                                                   >  2:458938/1‑150 (MQ=255)
                                                                                                 tCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGaaa                                                 >  4:1209777/1‑150 (MQ=255)
                                                                                                  ccTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTCCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGaaaaa                                               <  1:208307/151‑1 (MQ=255)
                                                                                                    tttGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGc                                             >  4:255565/1‑151 (MQ=255)
                                                                                                      tGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCCCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGc                                             >  4:747272/1‑149 (MQ=255)
                                                                                                       gTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAgg                                          <  4:743800/151‑1 (MQ=255)
                                                                                                       gTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAgg                                          >  1:666843/1‑151 (MQ=255)
                                                                                                         aTTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGcc                                        >  1:779120/1‑151 (MQ=255)
                                                                                                         aTTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGc                                         <  1:741798/150‑1 (MQ=255)
                                                                                                          ttGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCCCATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGcc                                        <  4:148589/150‑1 (MQ=255)
                                                                                                            gTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCAAATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGc                                     >  3:77478/1‑151 (MQ=255)
                                                                                                               gggATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCtat                                  <  1:1789319/151‑1 (MQ=255)
                                                                                                               gggATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCtat                                  >  2:422527/1‑151 (MQ=255)
                                                                                                                ggATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCtata                                 <  1:29827/151‑1 (MQ=255)
                                                                                                                       tGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATtgtg                            <  3:383593/149‑1 (MQ=255)
                                                                                                                       tGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTgg                           >  3:209472/1‑150 (MQ=255)
                                                                                                                          tGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAgg                       >  4:684321/1‑151 (MQ=255)
                                                                                                                           gATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGc                      >  4:443082/1‑151 (MQ=255)
                                                                                                                           gATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGc                      >  4:520463/1‑151 (MQ=255)
                                                                                                                            aTTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAg                        >  1:382105/1‑148 (MQ=255)
                                                                                                                            aTTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGc                      >  4:721733/1‑150 (MQ=255)
                                                                                                                              ttgtttgCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCaa                    <  1:870663/150‑1 (MQ=255)
                                                                                                                               tgtttgCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATg                  >  1:1648590/1‑151 (MQ=255)
                                                                                                                                 tttgACGTTTTCTTCATTTACCAGCGCCACCACCTCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGCTGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGAGGAAGGCAATGcc                >  1:1543589/1‑151 (MQ=255)
                                                                                                                                   tgCCGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGctg               >  1:1868250/1‑148 (MQ=255)
                                                                                                                                      cGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGaa             >  1:735783/1‑149 (MQ=255)
                                                                                                                                      cGTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGaa             >  4:822202/1‑149 (MQ=255)
                                                                                                                                       gTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAg           <  1:522089/150‑1 (MQ=255)
                                                                                                                                       gTTTTCTTCATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGc          <  2:190377/151‑1 (MQ=255)
                                                                                                                                          ttcttcATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACCCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCaa        <  3:26436/150‑1 (MQ=255)
                                                                                                                                           tcttcATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCaaa       >  4:190916/1‑150 (MQ=255)
                                                                                                                                           tcttcATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCaaa       >  4:1760674/1‑150 (MQ=255)
                                                                                                                                             ttcATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAgg    >  1:1847480/1‑151 (MQ=255)
                                                                                                                                               cATTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAggg   <  1:593770/150‑1 (MQ=255)
                                                                                                                                                aTTTACCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGgc  <  1:1159353/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGC  >  NC_000913/246636‑246929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: