Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,241,409 T→C Q87R (CAG→CGG)  galS ← galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor; mgl operon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,241,4090TC99.3% 530.7 / NA 140Q87R (CAG→CGG) galSgalactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor; mgl operon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (78/61);  minor base A (1/0);  total (79/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

ATCCATAAATTGCGCCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCT  >  NC_000913/2241261‑2241555
                                                                                                                                                    |                                                                                                                                                  
   cATAAATTGCGCCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGccgctg                                                                                                                                                >  1:1085125/1‑150 (MQ=255)
            ggcCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGAcc                                                                                                                                         >  3:301331/2‑148 (MQ=255)
            cgcCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGa                                                                                                                                      <  3:303494/151‑1 (MQ=255)
            cgcCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGa                                                                                                                                      <  2:293585/151‑1 (MQ=255)
              ccAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATc                                                                                                                                    <  4:1585631/151‑1 (MQ=255)
               cAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATcc                                                                                                                                   >  2:83644/1‑151 (MQ=255)
               cAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATcc                                                                                                                                   >  4:483227/1‑151 (MQ=255)
                aGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATcc                                                                                                                                   >  1:1367910/1‑150 (MQ=255)
                    cATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGc                                                                                                                              >  4:949682/1‑151 (MQ=255)
                    cATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGc                                                                                                                              >  4:738784/1‑151 (MQ=255)
                    cATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGc                                                                                                                              >  1:1784379/1‑151 (MQ=255)
                     aTCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCt                                                                                                                             >  1:181635/1‑151 (MQ=255)
                     aTCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCt                                                                                                                             >  4:1170745/1‑151 (MQ=255)
                        gTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCtttt                                                                                                                          <  1:317756/151‑1 (MQ=255)
                        gTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCtttt                                                                                                                          <  2:478938/151‑1 (MQ=255)
                         tCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCtttt                                                                                                                          >  1:1338020/1‑150 (MQ=255)
                          cACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTAc                                                                                                                        <  1:1639909/151‑1 (MQ=255)
                          cACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTAc                                                                                                                        <  4:627016/151‑1 (MQ=255)
                          cACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTAc                                                                                                                        >  1:468692/1‑151 (MQ=255)
                           aCTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTAcc                                                                                                                       >  3:264042/1‑151 (MQ=255)
                           aCTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTAcc                                                                                                                       >  2:358874/1‑151 (MQ=255)
                           aCTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTAcc                                                                                                                       <  4:1328174/151‑1 (MQ=255)
                            cTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTAcc                                                                                                                       >  4:458583/1‑150 (MQ=255)
                             tCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTAcc                                                                                                                       >  4:1343472/1‑149 (MQ=255)
                              cAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAg                                                                                                                     <  1:1273742/150‑1 (MQ=255)
                               aaTGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAgcg                                                                                                                   <  4:1250082/151‑1 (MQ=255)
                                 tGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAgcgcg                                                                                                                 >  4:612636/1‑151 (MQ=255)
                                  gCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAgcgcgc                                                                                                                >  4:312388/1‑151 (MQ=255)
                                      ttGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGa                                                                                                             >  4:49310/1‑150 (MQ=255)
                                      ttGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGa                                                                                                             <  2:13066/150‑1 (MQ=255)
                                      ttGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGa                                                                                                             <  4:1244493/150‑1 (MQ=255)
                                        gAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGaaaa                                                                                                          <  4:207205/151‑1 (MQ=255)
                                        gAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGaaa                                                                                                           <  1:1929357/150‑1 (MQ=255)
                                        gAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGaaa                                                                                                           <  1:1264410/150‑1 (MQ=255)
                                        gAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGaaa                                                                                                           <  4:1810547/150‑1 (MQ=255)
                                            gAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACg                                                                                                       <  1:1198458/150‑1 (MQ=255)
                                            gAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGc                                                                                                      <  1:1906340/151‑1 (MQ=255)
                                             aaCAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCa                                                                                                     <  1:617809/151‑1 (MQ=255)
                                                gaTCAAAGCATTACAGAGCTGGCGAATTAAAACCTCAAGGGCGTGACGCACTTTTTCAGTTTCATGATCGCTATTGCCGATTAGCACATATTTCTGATGCAGATGGGCGACCAGATCCACCGCTTTTACCATGGAGAAGAAAAACGCATc                                                                                                   >  3:381939/2‑150 (MQ=255)
                                                      cGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACg                                                                                            <  4:1081746/151‑1 (MQ=255)
                                                       gCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGt                                                                                           >  1:1863708/1‑151 (MQ=255)
                                                        cATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTc                                                                                          <  1:1046217/151‑1 (MQ=255)
                                                            aCAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATc                                                                                      >  4:371775/1‑151 (MQ=255)
                                                             cAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATca                                                                                     <  2:131869/151‑1 (MQ=255)
                                                               aCGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATcacc                                                                                   >  1:828195/1‑151 (MQ=255)
                                                                cGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTAGTGTCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCACCGCTCCGAAAAACGCATAACAAACGTCCGTcgcca                                                                                  <  1:1015029/151‑1 (MQ=255)
                                                                 gCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATcaccac                                                                                 >  1:1387239/1‑151 (MQ=255)
                                                                    ggCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATcaccaccac                                                                              <  3:64423/151‑1 (MQ=255)
                                                                      cGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGc                                                                            >  4:531187/1‑151 (MQ=255)
                                                                      cGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGc                                                                            >  4:1875136/1‑151 (MQ=255)
                                                                         aTTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGCAAAACGCATCAGAAACGTCCATCACCACCACGCCa                                                                          >  3:40472/1‑150 (MQ=255)
                                                                           tAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATg                                                                       >  1:1913094/1‑151 (MQ=255)
                                                                            aaCACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATgg                                                                      <  3:120341/151‑1 (MQ=255)
                                                                             acacCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCAGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGgt                                                                     >  1:1842687/1‑151 (MQ=255)
                                                                              cacCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGgtg                                                                    >  4:1174260/1‑151 (MQ=255)
                                                                                   cAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCg                                                                 <  1:484316/149‑1 (MQ=255)
                                                                                    aaTGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCt                                                               >  1:845764/1‑150 (MQ=255)
                                                                                    aaTGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTa                                                              <  4:1548577/151‑1 (MQ=255)
                                                                                    aaTGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTa                                                              <  4:432924/151‑1 (MQ=255)
                                                                                     aTGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTa                                                              >  4:1433185/1‑150 (MQ=255)
                                                                                      tGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAAc                                                            >  4:650331/1‑151 (MQ=255)
                                                                                      tGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAAc                                                            >  4:846220/1‑151 (MQ=255)
                                                                                       ggCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAAcc                                                           <  1:1708124/151‑1 (MQ=255)
                                                                                        gCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCt                                                          >  1:1167196/1‑151 (MQ=255)
                                                                                         cGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTg                                                         >  4:772564/1‑151 (MQ=255)
                                                                                         cGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTg                                                         <  1:1728250/151‑1 (MQ=255)
                                                                                              cGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGtt                                                     <  4:1586139/150‑1 (MQ=255)
                                                                                              cGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTg                                                    <  1:1509035/151‑1 (MQ=255)
                                                                                                   tttttCCGCTTCATGATAGTTATTGTCGATTAGCACGTATTTCTTATGCCGCTGCGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAg                                                >  3:63028/1‑150 (MQ=255)
                                                                                                   tttttCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAgc                                               >  2:78986/1‑151 (MQ=255)
                                                                                                    ttttCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAgcg                                              >  2:402969/1‑151 (MQ=255)
                                                                                                       tCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGcc                                            <  1:717017/150‑1 (MQ=255)
                                                                                                       tCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCt                                           >  4:263743/1‑151 (MQ=255)
                                                                                                          gCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTggg                                        >  1:102548/1‑151 (MQ=255)
                                                                                                          gCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTggg                                        <  4:1324496/151‑1 (MQ=255)
                                                                                                             tCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCAt                                     >  3:383656/1‑151 (MQ=255)
                                                                                                               aTGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTg                                   <  3:119720/151‑1 (MQ=255)
                                                                                                                  aTAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCg                                >  1:630914/1‑151 (MQ=255)
                                                                                                                   tAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGt                               >  4:1545548/1‑151 (MQ=255)
                                                                                                                    aGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAATCGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGt                               <  3:13582/150‑1 (MQ=255)
                                                                                                                     gCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGttt                             >  3:453992/1‑151 (MQ=255)
                                                                                                                      cTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTg                            >  4:1583876/1‑151 (MQ=255)
                                                                                                                      cTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTg                            >  2:296082/1‑151 (MQ=255)
                                                                                                                       tATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTgg                           >  2:465368/1‑151 (MQ=255)
                                                                                                                        aTTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTg                            <  2:181270/149‑1 (MQ=255)
                                                                                                                           gCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCg                        <  4:1156447/150‑1 (MQ=255)
                                                                                                                           gCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCg                        >  4:680220/1‑150 (MQ=255)
                                                                                                                            ccGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGAt                      >  4:1080374/1‑151 (MQ=255)
                                                                                                                             cGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGAt                      >  2:533185/1‑150 (MQ=255)
                                                                                                                                  aGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATcc                 <  3:157609/150‑1 (MQ=255)
                                                                                                                                  aGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATcc                 <  1:773718/150‑1 (MQ=255)
                                                                                                                                     aCGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGct             >  4:1183661/1‑151 (MQ=255)
                                                                                                                                     aCGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGct             >  3:439320/1‑151 (MQ=255)
                                                                                                                                         aTTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGctcact         <  1:581337/151‑1 (MQ=255)
                                                                                                                                           ttCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGctcactc        >  1:263786/1‑150 (MQ=255)
                                                                                                                                             cTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGCTCACTCACg     >  1:1695767/1‑151 (MQ=255)
                                                                                                                                              tGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGCTCACTCACgg    >  4:1695097/1‑151 (MQ=255)
                                                                                                                                               gATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGCTCACTCACg     <  1:1078072/149‑1 (MQ=255)
                                                                                                                                                aTGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCt  >  4:1391830/1‑151 (MQ=255)
                                                                                                                                                aTGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCt  >  4:122145/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATCCATAAATTGCGCCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCT  >  NC_000913/2241261‑2241555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: