Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,244,134 A→G V75V (GTA→GTG yeiG → S‑formylglutathione hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,244,1340AG99.3% 503.9 / NA 151V75V (GTA→GTGyeiGS‑formylglutathione hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (71/79);  minor base T (0/1);  total (71/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 151 total aligned reads displayed.

GCCCGATGACGTTCAGTATCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGAT  >  NC_000913/2243986‑2244278
                                                                                                                                                    |                                                                                                                                                 
  ccGATGACGTTCAGTATCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTgg                                                                                                                                               <  1:408974/151‑1 (MQ=255)
      tGACGTTCAGTATCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTg                                                                                                                                             >  1:1603724/1‑149 (MQ=255)
        aCGTTCAGTATCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGc                                                                                                                                         >  4:409615/1‑151 (MQ=255)
              aGTATCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGac                                                                                                                                    >  4:563542/1‑150 (MQ=255)
              aGTATCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGGGCCCAGAGGGTAGCGGCGGAACTGGGGAGTGTGCCGGTGATTcccggc                                                                                                                                    >  2:133652/1‑146 (MQ=255)
                 aTCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACAcc                                                                                                                                 >  4:1020095/1‑150 (MQ=255)
                  tCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGACCTGGGGATTGTGCTGGTGATGCCAGACAcc                                                                                                                                 <  3:384232/149‑1 (MQ=255)
                  tCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACAcc                                                                                                                                 >  2:460003/1‑149 (MQ=255)
                     ttCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCa                                                                                                                                <  3:153895/147‑1 (MQ=255)
                     ttCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCa                                                                                                                                <  1:1868650/147‑1 (MQ=255)
                      tctcCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGccc                                                                                                                            >  4:1077844/1‑150 (MQ=255)
                             ccACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTTTGCTGGGGATGCCAGACACCAGCCcg                                                                                                                           >  2:51785/1‑144 (MQ=255)
                             ccACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCgcggcg                                                                                                                    <  1:1793560/151‑1 (MQ=255)
                             ccACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCgcgg                                                                                                                      <  4:1758477/149‑1 (MQ=255)
                               aCCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCgcggcg                                                                                                                    >  1:815884/1‑149 (MQ=255)
                                ccTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAGCTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTTTGCTGGTGATGCCAGACACCAGCACGCGCGGCGaaa                                                                                                                 <  1:832578/151‑1 (MQ=255)
                                ccTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGaa                                                                                                                  >  2:544490/1‑150 (MQ=255)
                                   cGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAg                                                                                                               <  1:961212/150‑1 (MQ=255)
                                    gTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGt                                                                                                             <  1:1408026/151‑1 (MQ=255)
                                      gATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTg                                                                                                           <  4:1460559/151‑1 (MQ=255)
                                         cacaCTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGcc                                                                                                         >  1:615056/1‑150 (MQ=255)
                                         cacaCTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCa                                                                                                        <  1:592832/151‑1 (MQ=255)
                                          acacTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCaa                                                                                                       <  4:363745/151‑1 (MQ=255)
                                            acTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAac                                                                                                      >  4:1250655/1‑150 (MQ=255)
                                               ccGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAacgacg                                                                                                 <  1:33423/151‑1 (MQ=255)
                                               ccGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAacgac                                                                                                  <  1:1480145/150‑1 (MQ=255)
                                                cGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGATGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAacgacg                                                                                                 >  2:56121/1‑150 (MQ=255)
                                                  ccaccaGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATg                                                                                              <  4:867182/151‑1 (MQ=255)
                                                  ccaccaGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATg                                                                                              <  4:1672532/151‑1 (MQ=255)
                                                  ccaccaGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATg                                                                                              <  4:1760261/151‑1 (MQ=255)
                                                   caccaGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGc                                                                                            <  4:163012/151‑1 (MQ=255)
                                                     ccaGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCCGACACCAGCCCGCGCGGCGAAAAGGGTGCCAACGACGATGGc                                                                                            >  3:467972/1‑150 (MQ=255)
                                                     ccaGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCt                                                                                           <  1:1570496/151‑1 (MQ=255)
                                                     ccaGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGGGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGc                                                                                            >  1:1417234/1‑150 (MQ=255)
                                                          gCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGa                                                                                       <  1:962439/150‑1 (MQ=255)
                                                          gCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGa                                                                                       <  4:275991/150‑1 (MQ=255)
                                                          gCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGAt                                                                                      <  1:481803/151‑1 (MQ=255)
                                                          gCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGAt                                                                                      <  4:1553352/151‑1 (MQ=255)
                                                          gCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGAGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAAGACGATGGCTACGAt                                                                                      >  2:414001/1‑151 (MQ=255)
                                                            tGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGGTGCCAACGACGATGGCTACGAttt                                                                                    >  1:739061/1‑151 (MQ=255)
                                                              tACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGAttt                                                                                    >  2:343239/1‑149 (MQ=255)
                                                               aCTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAgg                                                                                 >  2:339487/1‑151 (MQ=255)
                                                               aCTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAg                                                                                  <  1:1129786/150‑1 (MQ=255)
                                                               aCTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAAATGGGGATTGTGCTGGTGATGCCCGACACCAGCCCGCGCGGCGGAAAGGTTGCCAACGACGATGGCTACGATTTAgg                                                                                 >  1:1340840/1‑151 (MQ=255)
                                                                cTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAgg                                                                                 >  2:386147/1‑150 (MQ=255)
                                                                 tGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGcc                                                                               >  4:1360532/1‑151 (MQ=255)
                                                                  ggCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGcc                                                                               >  4:749010/1‑150 (MQ=255)
                                                                   gCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGcc                                                                               >  1:525449/1‑149 (MQ=255)
                                                                     tttCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAggg                                                                           >  4:596390/1‑151 (MQ=255)
                                                                      ttCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAggg                                                                           <  4:780870/150‑1 (MQ=255)
                                                                      ttCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAggg                                                                           >  1:1664896/1‑150 (MQ=255)
                                                                      ttCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAgg                                                                            <  1:282735/149‑1 (MQ=255)
                                                                        ccGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCCGGgcg                                                                         >  1:1903588/1‑150 (MQ=255)
                                                                        ccGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGgcgc                                                                        <  1:922880/151‑1 (MQ=255)
                                                                        ccGGATAAACCTGCAATGACGAGAACGTCGCCACCAAGGCGGGTGCCCAGCGGGCAGGGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGGGCGGCGAAAAGGTTGCCAACTACGATGGCTCCGATTTAGGCCAGGgc                                                                          <  3:52583/149‑1 (MQ=255)
                                                                         cGGATTAACCTGCAATGACGATAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGTTTGCCAACGACGATTGCTACGATTTAGGCCAGGGAGCa                                                                       <  3:390303/151‑1 (MQ=255)
                                                                          ggATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGgcg                                                                         >  4:1463134/1‑148 (MQ=255)
                                                                              tAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCt                                                                   <  2:413871/150‑1 (MQ=255)
                                                                               aaCCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGTGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCt                                                                   <  2:20453/149‑1 (MQ=255)
                                                                               aaCCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCttt                                                                 >  1:1053330/1‑151 (MQ=255)
                                                                               aaCCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCttt                                                                 <  2:163834/151‑1 (MQ=255)
                                                                                 ccTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTa                                                               <  4:1823562/151‑1 (MQ=255)
                                                                                 ccTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTa                                                               <  4:1915089/151‑1 (MQ=255)
                                                                                   tGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTAt                                                              >  4:1080351/1‑150 (MQ=255)
                                                                                    gCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCt                                                            >  4:1743180/1‑151 (MQ=255)
                                                                                     cAATGACGAGAACTTCACCACCAAGGCGGGTGCCCCGCGGGTAGCGGCGGAAATGGGGATTTCGCCGGTGATGCCAGACACCAGCCCGCGCGGGGAAAAGGTTGCCCACGACGATGGCTTAAAATTAGGCCAGGGCGGAGGCCTTTTTCtt                                                           >  3:2276/1‑151 (MQ=255)
                                                                                       aTGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGGGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTa                                                          >  4:298615/1‑150 (MQ=255)
                                                                                         gACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTaa                                                         >  4:1409527/1‑149 (MQ=255)
                                                                                         gACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAAt                                                        <  1:1376085/150‑1 (MQ=255)
                                                                                         gACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATg                                                       <  4:1654457/151‑1 (MQ=255)
                                                                                             agaACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCAc                                                   >  1:186229/1‑151 (MQ=255)
                                                                                              gaACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACg                                                  <  4:1837280/151‑1 (MQ=255)
                                                                                                aCTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCa                                                <  4:1404741/151‑1 (MQ=255)
                                                                                                aCTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCa                                                <  2:161443/151‑1 (MQ=255)
                                                                                                   tCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCaa                                               <  1:438868/149‑1 (MQ=255)
                                                                                                      ccaccaAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAAccgcc                                          <  4:748335/151‑1 (MQ=255)
                                                                                                           aggggggggccccAGCGGGTGGGGGCGGAACTGGGGATTGTGCGGGGGATGCCAGACACCATCCCGCGGGGCGAAAAGGTTGCCAACGACGATGCCTGCATTTGAGGCCGGGGCCCAGGTTTTTTTCTAAATCCCCCGCAACCGCCGTgg                                      <  1:1239473/140‑1 (MQ=255)
                                                                                                           aaGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTggg                                     >  4:1749492/1‑151 (MQ=255)
                                                                                                                   tGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCAt                             >  1:1420390/1‑151 (MQ=255)
                                                                                                                     cccGGCGGGTGGCGGCGGAACTGGGGATTGTGCGGGTAATGCCAGACCCCACCCCGCGCGGAGAAAAGGTTGCCAACGACGAGGGCTACGATTTAGGCGGGGGCGCAGGTTTTTATCTTAAGCCCACGCAGCCGCCGTGGGCGACGCATTa                           <  1:390603/151‑1 (MQ=255)
                                                                                                                     cccAGCGGGTGGCGGCGGAACGGGGGATTGTGCTGGTGATGCCAGACCCCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATCCCACGCAACCGCCGTGGGCGACGCAt                             <  2:284626/149‑1 (MQ=255)
                                                                                                                       cAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTAcc                         >  4:1289176/1‑151 (MQ=255)
                                                                                                                        aGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCg                        >  1:345887/1‑151 (MQ=255)
                                                                                                                         gggggTAGCGGCGGAACGGGGGATGGTGCGGGTGAGGCCAGACACCAGCCCGCGCGGCGAAAGGGTTGCCAACGACGATGGCTACGTTTTAGGCCGGGGCGCAGGCTTTTATTTAAAGGCCACGCAACCGCCGTGGGCGACGCATTAcc                         <  1:1046945/147‑1 (MQ=255)
                                                                                                                         gCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCgg                       >  4:64591/1‑151 (MQ=255)
                                                                                                                          tgggTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCgg                       >  1:1504832/2‑150 (MQ=255)
                                                                                                                           gggTGGCGGCGGAACTGGGGATTGTGCGGGTGATCCCAGACCCCAGCCCGCGCGGAGAAAAGGTGCCCAACGACGATGGCTCCGATTTAGGCCGGGGCGCAGGTTTTTCTCTTACTCCCCCGCAACCGCCGTGGGCGACGCATTACCg                        <  1:780130/148‑1 (MQ=255)
                                                                                                                           gggTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGa                      >  1:921888/1‑150 (MQ=255)
                                                                                                                           gggTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGAt                     >  4:1057012/1‑151 (MQ=255)
                                                                                                                             gTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTAAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGt                   <  2:126441/151‑1 (MQ=255)
                                                                                                                               aGCGGGGAAACGGGGGATTGTGCTGGTGATGCCACACACCAGCCCGCGCGGAAAAAAGGTGGCAAACGAGGATGGCTAGGATTGAGGCCGGGGCGAGGGTTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTa                  <  1:510352/150‑1 (MQ=255)
                                                                                                                                  ggcggAACGGGGGTTTGGGCTGGTGAGGCCAGACACCGGCCCGCGGGGCGAAAGGGTGCCCACCGACGAGGGCTAGGATTTAGGCCGGGGCGAAGGCTTTTATTTTAAGGCCAGGCAACCGCCGTGGGCGACGCATTACCGGATGTATg                <  1:545034/149‑1 (MQ=255)
                                                                                                                                      gAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATc          >  1:770730/1‑151 (MQ=255)
                                                                                                                                        aCTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTg        >  1:357728/1‑151 (MQ=255)
                                                                                                                                           ggggATTGTGCTGGTGATGCCAGACACCAGCCGGCGGGGCGAAAAGGTTGCCAACGACGGTGGCTACGATTTAGGCCGGGGCGCGGGTTTTTATCTTAACGCCACGAAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTgc       <  4:977530/149‑1 (MQ=255)
                                                                                                                                           ggggATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTgcgc     >  4:1234261/1‑151 (MQ=255)
                                                                                                                                            gggTTTGTGCGGGGGAAGCAAGACCCGAGCCCGCGGGGAAAAGGGGTTCCAACTGAAGATGGCTAACATTAAGCCGCGGGCGAAGCTTTTTATTTAAATGCCACGAAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTgcg      <  4:199952/149‑1 (MQ=255)
                                                                                                                                            gggATGGTGCTGGTGATGCCAGACCCCAGCCGGCGCGGCGAAAAGTTGGCAAACGACGATGGCTACGATTTAGGCCGGGGCGCAGGCTTTTATCTTAATGCCACGCAGCCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGcgcg    <  1:1396668/151‑1 (MQ=255)
                                                                                                                                                ttGTGCTGGGGATCCCAGACACCGCCCCGCGGGGGGAAAAGGTTGCCAACGACGATGGCTACGATTTGGGCCGGGGGGAAGGTTTTTATCTTAATCCCCCGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGAt  <  4:719567/149‑1 (MQ=255)
                                                                                                                                                ttGTGCGGGTGATGCCAGACCCCAGCCCGCGCGGCAAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCGGGGCGCAGGTTTTTATTTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGAt  <  1:710117/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GCCCGATGACGTTCAGTATCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGAT  >  NC_000913/2243986‑2244278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: