Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,430,394 T→C T124A (ACC→GCC)  cvpA ← colicin V production protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,430,3940TC99.3% 499.4 / NA 136T124A (ACC→GCC) cvpAcolicin V production protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (71/63);  minor base G (1/0);  total (73/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

TTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGAT  >  NC_000913/2430247‑2430541
                                                                                                                                                    |                                                                                                                                                   
ttCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCaa                                                                                                                                                   <  2:281371/150‑1 (MQ=255)
  ccTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAgg                                                                                                                                                >  4:489625/1‑151 (MQ=255)
  ccTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAg                                                                                                                                                 <  3:280830/150‑1 (MQ=255)
  ccTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAg                                                                                                                                                 <  3:544187/150‑1 (MQ=255)
     cGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAg                                                                                                                                              <  1:729283/150‑1 (MQ=255)
     cGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGt                                                                                                                                             >  2:451653/1‑151 (MQ=255)
         aaGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCgag                                                                                                                                         <  4:1471792/151‑1 (MQ=255)
         aaGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCga                                                                                                                                          <  1:1051685/150‑1 (MQ=255)
          aGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCgaga                                                                                                                                        <  4:1762684/151‑1 (MQ=255)
            ccACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGagaa                                                                                                                                       <  4:1027177/150‑1 (MQ=255)
               caTCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGagaaaga                                                                                                                                    <  4:193538/150‑1 (MQ=255)
                 tctcAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGagaaaga                                                                                                                                    >  4:1738780/1‑148 (MQ=255)
                    cAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAagag                                                                                                                                <  4:1081982/149‑1 (MQ=255)
                          aCTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGgc                                                                                                                          >  2:84465/1‑149 (MQ=255)
                             tACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGgcagc                                                                                                                       <  1:539457/149‑1 (MQ=255)
                               cGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGcaaca                                                                                                                   <  1:1596267/151‑1 (MQ=255)
                                gCTCTGGGCAAGGAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGATGTGATTTGCTCCAGCCTTCGCTTTTCAACACCCCGGGAAAGGAGTCGAGAAAGAAGAGAATGgcagca                                                                                                                      >  3:332248/1‑147 (MQ=255)
                                     gggCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCaa                                                                                                              <  1:1203032/150‑1 (MQ=255)
                                      ggCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACTCCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAaca                                                                                                            <  4:1143067/151‑1 (MQ=255)
                                      ggCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAaca                                                                                                            <  4:1770301/151‑1 (MQ=255)
                                      ggCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAac                                                                                                             >  4:746558/1‑150 (MQ=255)
                                        cAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAacac                                                                                                           >  2:497965/1‑150 (MQ=255)
                                         aaGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAacacac                                                                                                         >  2:359080/1‑151 (MQ=255)
                                          aGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACTcc                                                                                                        <  1:1205011/151‑1 (MQ=255)
                                            aaaCTTGACGAGCTTTGCAGATAATCAAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACcg                                                                                                       >  4:844557/1‑151 (MQ=255)
                                            aaaCTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACcgc                                                                                                      >  4:1286831/1‑151 (MQ=255)
                                            aaaCTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACcgc                                                                                                      <  1:1512567/151‑1 (MQ=255)
                                            aaaCTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACcgc                                                                                                      >  1:553833/1‑151 (MQ=255)
                                              aCTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCgcgc                                                                                                    <  2:153869/151‑1 (MQ=255)
                                                ttGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCa                                                                                                   >  1:1110944/1‑150 (MQ=255)
                                                ttGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCa                                                                                                   >  4:583601/1‑150 (MQ=255)
                                                ttGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCa                                                                                                   >  4:640355/1‑150 (MQ=255)
                                                   aCGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGc                                                                                               <  1:475768/151‑1 (MQ=255)
                                                   aCGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGc                                                                                               >  4:1448871/1‑151 (MQ=255)
                                                     gAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCAc                                                                                             >  4:293643/1‑151 (MQ=255)
                                                     gAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCAc                                                                                             >  1:1779474/1‑151 (MQ=255)
                                                      aGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCAcc                                                                                            >  4:1503000/1‑151 (MQ=255)
                                                       gCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCg                                                                                           >  2:63295/1‑151 (MQ=255)
                                                       gCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCg                                                                                           <  1:922405/151‑1 (MQ=255)
                                                       gCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCg                                                                                           >  1:1614860/1‑151 (MQ=255)
                                                       gCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCg                                                                                           >  1:357257/1‑151 (MQ=255)
                                                          ttGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGaa                                                                                         >  1:1111659/1‑150 (MQ=255)
                                                              aGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAAc                                                                                       >  2:89309/1‑148 (MQ=255)
                                                               gATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGAc                                                                                   <  1:1144386/151‑1 (MQ=255)
                                                                       aaaaaaaCATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGc                                                                            >  1:676905/1‑151 (MQ=255)
                                                                        aaaaaaCATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGc                                                                            >  1:636031/1‑150 (MQ=255)
                                                                        aaaaaaCATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCa                                                                           <  4:739715/151‑1 (MQ=255)
                                                                         aaaaaCATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCAc                                                                          <  4:839400/151‑1 (MQ=255)
                                                                           aaaCATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCAccc                                                                        >  4:718563/1‑151 (MQ=255)
                                                                           aaaCATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCAccc                                                                        >  1:999743/1‑151 (MQ=255)
                                                                             aCATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCg                                                                       >  4:778314/1‑150 (MQ=255)
                                                                             aCATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGAAAAGGAGTCGAGAAAGAAGCGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCg                                                                       >  3:363329/1‑150 (MQ=255)
                                                                               aTCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATc                                                                    >  3:38984/1‑151 (MQ=255)
                                                                                tCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCg                                                                   >  1:1404409/1‑151 (MQ=255)
                                                                                tCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCg                                                                   >  4:32201/1‑151 (MQ=255)
                                                                                  tgatgaTAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGt                                                                 >  1:499839/1‑151 (MQ=255)
                                                                                   gatgatAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATc                                                                    <  3:462551/147‑1 (MQ=255)
                                                                                   gatgatAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTg                                                                >  4:984965/1‑151 (MQ=255)
                                                                                    atgatAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGc                                                               >  4:1564417/1‑151 (MQ=255)
                                                                                      gatAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCt                                                             <  4:84574/151‑1 (MQ=255)
                                                                                       atAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTg                                                            <  1:1078512/151‑1 (MQ=255)
                                                                                        tAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTg                                                            >  4:800194/1‑150 (MQ=255)
                                                                                           aaCTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACaa                                                        <  3:309417/151‑1 (MQ=255)
                                                                                            aCTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAAc                                                       >  2:396195/1‑151 (MQ=255)
                                                                                            aCTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAAc                                                       >  1:28777/1‑151 (MQ=255)
                                                                                              tGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAAccc                                                     >  4:1348191/1‑151 (MQ=255)
                                                                                              tGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAAccc                                                     >  4:951403/1‑151 (MQ=255)
                                                                                               gAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAAcccc                                                    <  1:1692170/151‑1 (MQ=255)
                                                                                                 aTTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGt                                                  >  4:1734101/1‑151 (MQ=255)
                                                                                                 aTTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGt                                                  <  1:549017/151‑1 (MQ=255)
                                                                                                   tGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGttt                                                >  1:675409/1‑151 (MQ=255)
                                                                                                     cGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTtc                                              >  1:7387/1‑151 (MQ=255)
                                                                                                         aTCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTcca                                          <  1:1875471/151‑1 (MQ=255)
                                                                                                         aTCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTcca                                          <  3:188259/151‑1 (MQ=255)
                                                                                                                gtgATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACt                                    <  1:1440042/150‑1 (MQ=255)
                                                                                                                  gATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGc                                 >  4:1438201/1‑151 (MQ=255)
                                                                                                                     ttGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCt                               <  1:741801/150‑1 (MQ=255)
                                                                                                                      tGCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCtc                              <  1:1081399/150‑2 (MQ=255)
                                                                                                                       gCTCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTAt                             <  4:1016763/150‑1 (MQ=255)
                                                                                                                         tCCAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCAc                          >  4:275805/1‑151 (MQ=255)
                                                                                                                          ccAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCa                           <  1:1738101/149‑1 (MQ=255)
                                                                                                                          ccAGTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCAc                          <  1:470189/150‑1 (MQ=255)
                                                                                                                             gTCTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGa                        >  2:299840/1‑149 (MQ=255)
                                                                                                                               cTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGtt                    >  4:1090910/1‑151 (MQ=255)
                                                                                                                               cTTCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGtt                    >  2:393750/1‑151 (MQ=255)
                                                                                                                                 tCGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGtt                    <  1:76149/149‑1 (MQ=255)
                                                                                                                                  cGCTTTTCGACGCCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCAc                 >  1:1670653/1‑151 (MQ=255)
                                                                                                                                  cGCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCAc                 >  4:1029262/1‑151 (MQ=255)
                                                                                                                                   gCTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACg                <  2:206310/151‑1 (MQ=255)
                                                                                                                                    cTTTTCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACg                >  2:446011/1‑150 (MQ=255)
                                                                                                                                     ttttCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGAt              >  1:877384/1‑151 (MQ=255)
                                                                                                                                      tttCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGAt              >  2:167098/1‑150 (MQ=255)
                                                                                                                                        tCGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGc           >  4:217313/1‑151 (MQ=255)
                                                                                                                                         cGACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCa          <  2:117908/151‑1 (MQ=255)
                                                                                                                                          gACACCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCAc         >  1:1584281/1‑151 (MQ=255)
                                                                                                                                             acCCCGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCaa      <  1:1493786/151‑1 (MQ=255)
                                                                                                                                               cccGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACg    <  1:1365412/151‑1 (MQ=255)
                                                                                                                                                ccGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACg    >  1:187180/1‑150 (MQ=255)
                                                                                                                                                ccGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGa   <  3:236168/151‑1 (MQ=255)
                                                                                                                                                 cGGCAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGAt  >  1:1409470/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGAT  >  NC_000913/2430247‑2430541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑