BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	401,576	Δ1 bp	coding (191/261 nt)	iraP →	anti‑RssB factor, RpoS stabilzer during Pi starvation; anti‑adapter protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	401,576	0	G	.	100.0%	581.3 / NA	118	coding (191/261 nt)	iraP	anti‑RssB factor, RpoS stabilzer during Pi starvation; anti‑adapter protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (53/65); total (53/65)

Only 100 of 118 total aligned reads displayed.

AGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCTTTGTTTTTATTTTTT  >  NC_000913/401430‑401719

  aagaagaGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCt                                                                                                                                          >  4:533331/1‑151 (MQ=255)

  aagaagaGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCt                                                                                                                                          >  2:788779/1‑151 (MQ=255)

        agTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTgacgac                                                                                                                                    <  1:769909/151‑1 (MQ=255)

         gTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGacgacg                                                                                                                                   <  1:1017757/151‑1 (MQ=255)

         gTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGacgacg                                                                                                                                   <  1:1529853/151‑1 (MQ=255)

              aaGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACg                                                                                                                              <  1:383156/151‑1 (MQ=255)

                 aaCTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACgg                                                                                                                             >  2:1174881/1‑149 (MQ=255)

                  aCTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAgc                                                                                                                          <  2:866177/151‑1 (MQ=255)

                   cTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAgct                                                                                                                         >  2:572520/1‑151 (MQ=255)

                     gtgtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAgct                                                                                                                         <  1:1027929/149‑1 (MQ=255)

                     gtgtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAgct                                                                                                                         <  1:304377/149‑1 (MQ=255)

                      tgtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAgctgct                                                                                                                      >  1:1663621/1‑151 (MQ=255)

                       gtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGctgctg                                                                                                                     >  2:11737/1‑151 (MQ=255)

                        tgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCtgctgc                                                                                                                    >  1:1110625/1‑151 (MQ=255)

                          cgcAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTgcgc                                                                                                                  >  3:23637/1‑151 (MQ=255)

                          cgcAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGGTGGTTGATCAGGGAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATAAGGAGctgctg                                                                                                                     >  1:765450/1‑148 (MQ=255)

                            cAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGa                                                                                                                <  1:835881/151‑1 (MQ=255)

                               gTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                                                               >  1:1221174/1‑149 (MQ=255)

                                tAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGTCGCAAAATTACCAACAGCGGTTGATTGATCAGGTAGAGGGAGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGa                                                                                                                <  1:617106/147‑1 (MQ=255)

                                 aGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACg                                                                                                           >  4:302966/1‑151 (MQ=255)

                                   aaGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGt                                                                                                          >  2:958763/1‑150 (MQ=255)

                                    aGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTa                                                                                                         >  1:439670/1‑150 (MQ=255)

                                      cTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTaa                                                                                                        >  2:1387638/1‑149 (MQ=255)

                                       tttGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTaa                                                                                                        >  3:464192/1‑148 (MQ=255)

                                        ttGGAGATTATCGTCACTGCGATGCTTGGCAATATGGCGCAAAATGACCAAGAGCGGTTGATTTATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CAGTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTaaa                                                                                                       <  1:313292/148‑1 (MQ=255)

                                        ttGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAaaga                                                                                                     <  3:24305/150‑1 (MQ=255)

                                           gagaTTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGtt                                                                                                 <  2:63107/151‑1 (MQ=255)

                                            agaTTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGtt                                                                                                 <  1:571516/150‑1 (MQ=255)

                                             gaTTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTAt                                                                                               <  4:215611/151‑1 (MQ=255)

                                              aTTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTAtt                                                                                              <  1:432958/151‑1 (MQ=255)

                                              aTTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAAAGACAAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTACTGACGACGATACGGAGCTGCTGCGCGATTACGTAaagaag                                                                                                   >  1:277899/1‑146 (MQ=255)

                                               ttATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTg                                                                                             >  2:1281533/1‑151 (MQ=255)

                                                tATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTg                                                                                             >  1:770588/1‑150 (MQ=255)

                                                 aTCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGaa                                                                                           <  2:219419/151‑1 (MQ=255)

                                                 aTCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGa                                                                                            <  1:206143/150‑1 (MQ=255)

                                                          gCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTc                                                                                   <  3:321294/150‑1 (MQ=255)

                                                          gCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTc                                                                                   <  1:1492681/150‑1 (MQ=255)

                                                            aaTGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTc                                                                                <  2:464497/151‑1 (MQ=255)

                                                             aTGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGCGGCGCTGTACGAGGAAAAGACCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTc                                                                                >  3:28879/1‑150 (MQ=255)

                                                               gCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCt                                                                                    <  4:362383/144‑1 (MQ=255)

                                                               gCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCt                                                                                    >  3:362402/1‑144 (MQ=255)

                                                                cTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGt                                                                             >  2:1101287/1‑150 (MQ=255)

                                                                  tCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTa                                                                            <  2:1088506/149‑1 (MQ=255)

                                                                  tCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTa                                                                            <  4:365148/149‑1 (MQ=255)

                                                                   cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTaaa                                                                          >  2:518703/1‑150 (MQ=255)

                                                                      aaTATGGCGCAAAATGACCATCAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGGAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAATCATCCTCGTCAGTaaaaa                                                                        <  1:430863/149‑1 (MQ=255)

                                                                       atatGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCTATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGtt                                                                     <  2:62944/151‑1 (MQ=255)

                                                                        tatGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGtt                                                                     >  2:970539/1‑150 (MQ=255)

                                                                        tatGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGtt                                                                     >  1:1672636/1‑150 (MQ=255)

                                                                         atGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTa                                                                    <  1:1229156/150‑1 (MQ=255)

                                                                              gcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCttt                                                              >  1:954306/1‑151 (MQ=255)

                                                                                 aaaTGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTc                                                            >  2:554023/1‑150 (MQ=255)

                                                                                       ccAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCt                                                     >  2:856989/1‑151 (MQ=255)

                                                                                        cAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGATGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTg                                                    >  1:1662201/1‑151 (MQ=255)

                                                                                        cAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTg                                                    <  2:1310294/151‑1 (MQ=255)

                                                                                          aCAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTc                                                  <  2:1066639/151‑1 (MQ=255)

                                                                                           cAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCa                                                 <  1:163993/151‑1 (MQ=255)

                                                                                            aGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCAt                                                <  1:634619/151‑1 (MQ=255)

                                                                                             gCGGTTGACTGATCAGGTAGAGGGGGCGCTGTACGAGCTAAAGCCCGATGCCA‑CATTCCTGACGACGATTCGGATCTGCTGCGCGACTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGATAATCTTTTCAACAGCAGTCAt                                                <  1:9787/150‑1 (MQ=255)

                                                                                              cGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATaa                                              <  1:1387487/151‑1 (MQ=255)

                                                                                               ggTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATaa                                              >  4:377030/1‑150 (MQ=255)

                                                                                                 ttgattgaTCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGt                                           >  1:1152488/1‑151 (MQ=255)

                                                                                                      tgatCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTc                                       >  4:502440/1‑150 (MQ=255)

                                                                                                      tgatCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTc                                       >  3:442253/1‑150 (MQ=255)

                                                                                                       gatCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCa                                      <  1:13442/150‑1 (MQ=255)

                                                                                                        atCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACg                                    >  4:571280/1‑151 (MQ=255)

                                                                                                        atCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACg                                    <  2:1315583/151‑1 (MQ=255)

                                                                                                         tCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACgg                                   >  2:404125/1‑151 (MQ=255)

                                                                                                          cAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACgg                                   <  4:368383/150‑1 (MQ=255)

                                                                                                            ggTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCg                                >  4:479971/1‑151 (MQ=255)

                                                                                                            ggTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCg                                <  4:214322/151‑1 (MQ=255)

                                                                                                            ggTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCg                                <  1:480757/151‑1 (MQ=255)

                                                                                                              tAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCg                                >  2:1706729/1‑149 (MQ=255)

                                                                                                              tAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCg                                >  2:1610124/1‑149 (MQ=255)

                                                                                                                  gggggCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACtt                          <  1:1110168/151‑1 (MQ=255)

                                                                                                                   ggggCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTta                         <  2:1309882/151‑1 (MQ=255)

                                                                                                                   ggggCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTta                         <  4:250272/151‑1 (MQ=255)

                                                                                                                    gggCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTtat                        <  2:724315/151‑1 (MQ=255)

                                                                                                                    gggCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTtat                        <  2:386597/151‑1 (MQ=255)

                                                                                                                    gggCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTtat                        >  3:586982/1‑151 (MQ=255)

                                                                                                                    gggCGCTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTtat                        <  2:30029/151‑1 (MQ=255)

                                                                                                                        gcTGTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTTATAGTc                    <  1:754542/151‑1 (MQ=255)

                                                                                                                           gTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGc                  <  2:1701389/150‑1 (MQ=255)

                                                                                                                           gTACGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCt                 <  2:1110760/151‑1 (MQ=255)

                                                                                                                             aCGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCttt               <  1:250171/151‑1 (MQ=255)

                                                                                                                             aCGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCtt                <  1:607113/150‑1 (MQ=255)

                                                                                                                              cGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCTTTg              <  2:1517637/151‑1 (MQ=255)

                                                                                                                              cGAGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCTTTg              <  2:850208/151‑1 (MQ=255)

                                                                                                                                aGGTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCTTTGtt            >  3:160403/1‑151 (MQ=255)

                                                                                                                                 ggTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCTTTGttt           <  1:788674/151‑1 (MQ=255)

                                                                                                                                 ggTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCTTTGttt           >  2:1042705/1‑151 (MQ=255)

                                                                                                                                  gTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCTTTGtttt          <  1:523063/151‑1 (MQ=255)

                                                                                                                                  gTAAAGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCTTTGtttt          <  1:2322/151‑1 (MQ=255)

                                                                                                                                      aGCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCTTTGTTTTTAtt      >  2:1692851/1‑151 (MQ=255)

                                                                                                                                       gCCCGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCTTTGTTTTTAttt     <  2:654940/151‑1 (MQ=255)

                                                                                                                                        cccGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCTTTGTTTTTAtttt    >  4:506665/1‑151 (MQ=255)

                                                                                                                                          cGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCTTTGTTTTTAtttttt  <  1:376895/151‑1 (MQ=255)

                                                                                                                                          cGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCTTTGTTTTTAtttttt  >  1:1160830/1‑151 (MQ=255)

                                                                                                                                          cGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCTTTGTTTTTAtttttt  <  1:847848/151‑1 (MQ=255)

                                                                                                                                          cGATGCCA‑CATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCTTTGTTTTTAtttttt  <  1:160480/151‑1 (MQ=255)

AGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCTTTGTTTTTATTTTTT  >  NC_000913/401430‑401719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`