Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,606,215 (G)8→7 noncoding (72/87 nt) leuP ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,2080G.96.8% 535.3 / 7.2 126noncoding (79/87 nt)leuPtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base . (57/65);  total (59/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

GAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAG  >  NC_000913/4606060‑4606353
                                                                                                                                                     |                                                                                                                                                 
  aaGGAAGTAAAGATAATTGGTGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATAGTCATCTTACTTTTTTTGATATCCCCTCGCTTGGTGCCAggct                                                                                                                                                >  2:865277/1‑148 (MQ=255)
       aGTAAAGATAATTGGTGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCATCGTTTGGTGCGAgggggggact                                                                                                                                          >  2:1016380/1‑148 (MQ=255)
         tAAAGATAATTGGTGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggggggactt                                                                                                                                         <  2:1013361/150‑5 (MQ=255)
          aaaGATAATTGGTGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGCCACTAACACCTGAAGCTATCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAGCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGTGACTTGa                                                                                                                                      <  1:1705295/151‑1 (MQ=21)
          aaaGATAATTGGTGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCTCCTCGTTTGGTGCGA‑GGGGGGGACTTg                                                                                                                                       >  1:1470793/1‑150 (MQ=255)
           aaGATAATTGGTGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGa                                                                                                                                      >  2:752146/1‑150 (MQ=255)
             gATAATTGGTGCGAGGGGGGGGCACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAAc                                                                                                                                    >  2:1170670/1‑151 (MQ=255)
              aTAATTGGTGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAAccc                                                                                                                                  <  4:72940/151‑1 (MQ=255)
               tAATTGGTGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAAcccc                                                                                                                                 >  2:398161/1‑151 (MQ=255)
               tAATTGGTGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCGGAAGCTAGCGCGTATACCAATTCCGCCACCTTCGCTCAGTCATCTTACTTTTTTTGATATCGCCTAGTTTGGTGCGA‑GGGGGGGACTTGCAcc                                                                                                                                   >  1:687294/1‑149 (MQ=21)
                aaTTGGTGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAAccccc                                                                                                                                <  4:453204/151‑1 (MQ=255)
                aaTTGGTGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGCCATATTACTTTTTTTGATATCGCCCCGTTTGGGGCGAgaaggggacc                                                                                                                                          >  1:760461/1‑139 (MQ=17)
                 aTTGGTGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAAccccc                                                                                                                                >  2:1089472/1‑150 (MQ=255)
                   tGGTGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCAc                                                                                                                              <  2:947519/150‑1 (MQ=255)
                    ggTGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACg                                                                                                                             <  2:978334/150‑1 (MQ=255)
                    ggTGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGt                                                                                                                            <  1:1644664/151‑1 (MQ=255)
                     gTGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTc                                                                                                                           <  2:1673923/151‑1 (MQ=255)
                     gTGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTc                                                                                                                           >  2:1187972/1‑151 (MQ=255)
                      tGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTcc                                                                                                                          >  2:591845/1‑151 (MQ=255)
                      tGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATATTACTTTTTTTGATATCGCCTCGTTTGGGGCGA‑GGGGGGGACTTGAACCCCCACGTcc                                                                                                                          >  1:1637761/1‑151 (MQ=255)
                           gggggggg‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                                     <  1:702119/151‑1 (MQ=255)
                                    aCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                             <  2:1525183/151‑1 (MQ=255)
                                    aCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTa                                                                                                              >  2:747257/1‑150 (MQ=255)
                                        gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                                         >  3:350242/1‑151 (MQ=255)
                                        gAACCCCCACGGCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTACGCACAGTCATCTTACTTTTTTTGATATCACCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                                         <  1:154581/151‑1 (MQ=255)
                                         aaCCGCCACGTCCGTAAGGACACAAATACCTGAAGCTAGCGCGTCTACCAATTCCGGCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACTCCt                                                                                                        <  4:323009/151‑1 (MQ=37)
                                           cccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACACCCATAATACTTTTATCGAAATCGCGTCGTTTAGGGCGA‑GGGTGTAACCTAAACCGCCAATTCCGTAAGAACACTAACACCt                                                                                                        >  1:65304/1‑149 (MQ=12)
                                            ccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGaa                                                                                                     <  3:17531/151‑1 (MQ=255)
                                            ccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGaa                                                                                                     <  2:1408632/151‑1 (MQ=255)
                                             cccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGa                                                                                                      >  3:148910/1‑149 (MQ=255)
                                                aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGCGGGGACTTGTACCCCCACGTCCGTAAGAAAATTAACACCTGAAg                                                                                                    >  1:1642174/1‑148 (MQ=37)
                                                 cGTCCGTAAGGCCACTAACATCTGAAGCTAGCGCGTATACCAATTCCGCCACCATCGCACAGTCAGCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACCCCTGAAGCTa                                                                                                 <  1:1130134/150‑1 (MQ=37)
                                                    ccGTAAGGACACTAACACCTGAAGCTAGCGCGTCTTCCAATTCCGCCACCTTCGCACAGTCATCTTACTTATTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                                                                              <  1:59761/150‑1 (MQ=255)
                                                         aGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                                                                         >  2:975264/1‑150 (MQ=255)
                                                          ggACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAc                                                                                       >  2:235989/1‑151 (MQ=255)
                                                                tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCTCACGTCCGAAAGAACACTAACACCAGAAGCTAGCTCGTCTACCAATTc                                                                                 >  4:211413/1‑151 (MQ=37)
                                                                 accacCTGAAGCTAGCGCGTCGACCAATTCCGCCACCTTCGGACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                                                <  1:1055809/149‑1 (MQ=255)
                                                                 aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                                                                                  <  2:1631551/149‑1 (MQ=255)
                                                                       tGAAGTTAGCGTGTCTACCAATTCCGCCTCCTTCGCACAGTCATCTTACTTTTTTTGATCTCGCCTAGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa                                                                                    <  1:934013/141‑1 (MQ=25)
                                                                        gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                                                                         >  2:929238/1‑151 (MQ=255)
                                                                             tatctcgTATACCAATTCCGCCTCCATCGCCCAGTCATCTTCCTTTTTTTGATATCGCCTCATTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACTCCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                                      <  1:743415/144‑1 (MQ=18)
                                                                             tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                    >  2:855258/1‑151 (MQ=255)
                                                                              aGCGCGTCTACCTACCCCGCCACCTGCGCGCACTCTTCTTACATTTATTGATTTCGGCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                                      <  1:240730/148‑1 (MQ=17)
                                                                               gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                                                                     >  1:521437/1‑148 (MQ=255)
                                                                                     cTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGCTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                                             >  1:467915/1‑150 (MQ=255)
                                                                                      tttcAATTCCGGCTGATTCGCACAGGCATAATCAATATTTTGATATCGCATCATTTGGTGCG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa                                                            <  1:102209/147‑1 (MQ=14)
                                                                                      tACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                                             >  2:988167/1‑149 (MQ=255)
                                                                                       aCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCCCCTTCGCACAGTGCg                                                             >  1:1484834/1‑148 (MQ=255)
                                                                                       aCCAATTCCGCCACCTTCGCACAGTCATATTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGAATTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa                                                            >  1:1449211/1‑149 (MQ=255)
                                                                                        ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCa                                                         <  4:542766/151‑1 (MQ=255)
                                                                                          aaTTCCGCCTCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCATTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAat                                                       <  1:674341/151‑1 (MQ=255)
                                                                                              ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATc                                                    <  2:1040071/150‑1 (MQ=255)
                                                                                              ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATc                                                    >  3:51392/1‑150 (MQ=255)
                                                                                              ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGCGCGAGCActat                                                     >  1:656932/1‑149 (MQ=255)
                                                                                                gactgcatagCACAGTCACATTACCTGTTTGGATATCGCATCGTTTGGGGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTGCCAATTCCGCCGCCTGCGCCCAGTGCGAGAAATATCaa                                                  <  1:950288/141‑1 (MQ=17)
                                                                                                gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                                 <  1:836081/151‑1 (MQ=255)
                                                                                                  ccccTTCGCACAGTCATCTTACATTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                                               <  1:584818/149‑1 (MQ=255)
                                                                                                       tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGCGCGAGCAATATCAACGTGGttt                                          >  1:1569950/1‑151 (MQ=255)
                                                                                                       tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGCGCGAGCAATATCAAAGAGGtt                                           >  1:1196646/1‑150 (MQ=21)
                                                                                                          cacaGTCATTTTCCTGTTTGTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAGCGTGGTTTTTg                                       <  1:1492832/151‑1 (MQ=21)
                                                                                                          cacaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTg                                       >  4:159631/1‑151 (MQ=255)
                                                                                                            caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGttttt                                        >  1:469372/1‑148 (MQ=255)
                                                                                                            caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                                     >  2:259752/1‑151 (MQ=255)
                                                                                                            caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                                     <  3:584021/151‑1 (MQ=255)
                                                                                                            caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGCGCGAACAATATAAACGAAGtttt                                         >  3:447481/1‑147 (MQ=18)
                                                                                                             aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                                    >  2:1162305/1‑151 (MQ=255)
                                                                                                             aGTCAGCTTACTTATAGTGATATCGCATCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                                    <  1:1514816/151‑1 (MQ=21)
                                                                                                                 aTCTTACTTTTTTTGATATCGCCTCGTTTGTTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCACCGTGGTTTTTGGTGCg                                  >  2:755786/1‑149 (MQ=255)
                                                                                                                 aTCTTACTTTTTTTGAGAACGCCTCGTTTGGGGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                                 <  1:217020/150‑1 (MQ=255)
                                                                                                                  tCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgg                               >  3:319736/1‑151 (MQ=255)
                                                                                                                   cTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggg                              >  1:1334675/1‑151 (MQ=255)
                                                                                                                     ttcTTTTTTTGATATCACCTCGTTTGGGGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggg                             <  1:549753/148‑1 (MQ=255)
                                                                                                                         ttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggggg                         >  2:446448/1‑150 (MQ=255)
                                                                                                                         ttttttGATATCGCCACGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggggg                          <  1:505985/150‑1 (MQ=255)
                                                                                                                          tttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGa                        <  2:164228/150‑1 (MQ=255)
                                                                                                                            tttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACtt                     >  2:963089/1‑151 (MQ=255)
                                                                                                                            tttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACt                      <  2:987536/150‑1 (MQ=255)
                                                                                                                             ttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACtt                     <  1:903136/150‑1 (MQ=255)
                                                                                                                             ttGATAACGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGCAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACtt                     <  1:924369/150‑1 (MQ=255)
                                                                                                                               gATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCCCCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTg                    <  2:863782/149‑1 (MQ=255)
                                                                                                                               gATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGa                   <  3:159652/150‑1 (MQ=255)
                                                                                                                                atatCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCTCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTGTTGGTGCGAGGGGGGGGACTTGa                   <  1:1581866/149‑1 (MQ=255)
                                                                                                                                atatCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAc                 >  2:418176/1‑151 (MQ=255)
                                                                                                                                atatCGCATCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGaa                  <  4:576366/150‑1 (MQ=255)
                                                                                                                                 aatCGCCTCGTTTGGTGCGA‑GGGGGGGACCTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTATACCAATACCGCCACCCTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAcc                <  1:855393/150‑1 (MQ=25)
                                                                                                                                   tCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATAAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAcccc              >  4:149387/1‑151 (MQ=255)
                                                                                                                                     gCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAccccc             >  2:1225270/1‑150 (MQ=255)
                                                                                                                                      cattgTTGGGTGCGC‑GGGGTGGACTTGAACCCCCACGTCCGTAAGAAAACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCAc           <  1:687097/147‑1 (MQ=25)
                                                                                                                                         cGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACg          <  3:465803/149‑1 (MQ=255)
                                                                                                                                          gTTTTGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTcc       <  4:448482/151‑1 (MQ=255)
                                                                                                                                          gTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAccccc             <  4:292405/145‑1 (MQ=255)
                                                                                                                                          gTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTcc       >  2:661107/1‑151 (MQ=255)
                                                                                                                                           tttGGTGCGA‑GGGGGGGACTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTCCCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTTCGAGGGGGGGGACTTGAAccccct            <  4:240867/145‑2 (MQ=255)
                                                                                                                                           tttGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGt         <  2:646539/148‑1 (MQ=255)
                                                                                                                                           tttGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATAAACGTGGTTTTTGGTGCGAGGGGGGGTACTTGAACCCCCACGt         >  1:347300/1‑148 (MQ=255)
                                                                                                                                            ttGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCg      <  2:233753/150‑1 (MQ=255)
                                                                                                                                              ggTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTa    <  1:1706805/150‑1 (MQ=255)
                                                                                                                                              ggTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTGCGAGCAATAACAACGAGGTTCTTGCTCCGAGGCGGGTGCCTGGAACCCCCACCTctgca    >  1:679048/1‑146 (MQ=17)
                                                                                                                                               gTGCGA‑GGGGGGGACTTGAACCCCCCCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAg  <  1:1724943/151‑1 (MQ=255)
                                                                                                                                               gTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAg  <  3:9923/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAG  >  NC_000913/4606060‑4606353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑