Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 121,578 (T)7→6 intergenic (‑27/‑514) aroP ← / → pdhR aromatic amino acid transporter/pyruvate dehydrogenase complex repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913121,5720T.88.0% 496.3 / 45.5 133intergenic (‑21/‑520)aroP/pdhRaromatic amino acid transporter/pyruvate dehydrogenase complex repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (16/0);  new base . (45/72);  total (61/72)
Fisher's exact test for biased strand distribution p-value = 1.13e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 142 total aligned reads displayed.

CGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCGAATGCACCCGCTT  >  NC_000913/121422‑121717
                                                                                                                                                      |                                                                                                                                                 
 ggACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGgttgtt                                                                                                                                                  <  1:1142537/151‑1 (MQ=255)
  gACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAAGGCCCGCTTTAGCTGCTCGCCGTGGTGTTGACCCTTCATCATGAAAACTCGTGCGGGGGGTGttt                                                                                                                                                 >  4:354820/1‑151 (MQ=255)
        aTTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGttttttgat                                                                                                                                           <  1:55559/151‑4 (MQ=255)
        aTTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAAACCGCGCTTTAGCTGCTCGCCGCGCTGTTTACCTTCCATCATGAAACCTCCTGCGGGGGGTGgttttt                                                                                                                                              >  4:284876/1‑148 (MQ=255)
           aCGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTa                                                                                                                                       <  3:596327/151‑1 (MQ=255)
               aGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCa                                                                                                                                   <  4:327605/151‑1 (MQ=255)
               aGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCa                                                                                                                                   <  1:1363285/151‑1 (MQ=255)
               aGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCa                                                                                                                                   <  2:1166080/151‑1 (MQ=255)
                      aCCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGAt                                                                                                                              <  2:1604543/149‑1 (MQ=255)
                      aCCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGAt                                                                                                                              <  2:1453872/149‑1 (MQ=255)
                        ccAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCgt                                                                                                                          <  2:1562701/151‑1 (MQ=255)
                        ccAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGACGCgt                                                                                                                          <  3:562339/151‑1 (MQ=255)
                          aGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTTAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGGCCTTCCCTCATGAAAACCCCTGCGGGGGGTGTTTTTTTATCTACGCCGTGGTGCg                                                                                                                           >  2:1337589/1‑148 (MQ=255)
                             aaTAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTa                                                                                                                     <  1:215987/151‑1 (MQ=255)
                             aaTAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAAGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGGGGGTGTTTTTTTATCTACGCAGTGATGCgtgtgt                                                                                                                      >  2:99459/1‑150 (MQ=255)
                                aaCCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGGGGGTGTTTTTTTATCTACGCAGTGATGCGTGTGTaa                                                                                                                    >  3:251596/1‑149 (MQ=255)
                                    cGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTg                                                                                                               <  1:589685/150‑1 (MQ=255)
                                     ggTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCa                                                                                                             <  2:714223/151‑1 (MQ=255)
                                     ggTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCa                                                                                                             <  2:1568878/151‑1 (MQ=255)
                                      gTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCaa                                                                                                            <  3:228391/151‑1 (MQ=255)
                                      gTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCaa                                                                                                            <  2:898978/151‑1 (MQ=255)
                                        cccTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAAtt                                                                                                          <  1:597282/151‑1 (MQ=255)
                                            aTCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTcc                                                                                                        <  3:322338/149‑1 (MQ=255)
                                            aTCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGt                                                                                                      <  1:1661983/151‑1 (MQ=255)
                                            aTCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGt                                                                                                      <  1:1336840/151‑1 (MQ=255)
                                            aTCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGt                                                                                                      <  1:560204/151‑1 (MQ=255)
                                              cgcgCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGttt                                                                                                    <  3:192029/151‑1 (MQ=255)
                                              cgcgCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGttt                                                                                                    <  3:434713/151‑1 (MQ=255)
                                              cgcgCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAAGCCGCGCTTTAGCTGCTCGCCGGGCTGTTGACCTTCCCTCATGAAACCTCGTGCGGGGGTTGTTTTTTTATCTACGCAGTGATGCGGGTGTAAGTTTGCAATTCCGttt                                                                                                    >  2:1535877/1‑151 (MQ=255)
                                               gcgcCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTTAGGGCGCGCTTTTTCTGCCCGCCGTGCTGTTTACCTTCCATTATGAAAACTCGTGCGGTGGTTGTTTTTTTTTCTACGCCGTGTTGCGTGTGTAAGGTTTTAAATCCGttt                                                                                                    >  4:390614/1‑150 (MQ=255)
                                               gcgcCACCCAGCGCGATAAGCTGAATATGGCGGGTTTTTAGGCCCCGCTTTAGCTGCCCGCCGTGGTGGTGGGCTTCCATCATGAAAACTCCTGCGGTGGTTGTTTTTTTATCTACGCAGTGATGCGTGTGTAAGATTGCAATTCCGt                                                                                                      >  2:1581003/1‑148 (MQ=255)
                                                 gcCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTttgtt                                                                                                 <  1:495752/151‑1 (MQ=255)
                                                    aCCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTa                                                                                              <  1:1423979/151‑1 (MQ=255)
                                                    aCCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAAGCCGCGCTTTAGCTGCTCGCCGTGCTGTTTACCTTCCATCATGAAACCTCGTGCGGGGGGTGTTTTTTTATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTtgttgt                                                                                               >  2:793113/1‑150 (MQ=255)
                                                     cccAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTAt                                                                                             <  1:1414067/151‑1 (MQ=255)
                                                       cAGCGCGATAAGCTGAATATGGCGGTTTTTTAGGCCCCGCTTTAGCTGCCCGCCGTGCTGTTGTCCTTCCCTCATGAAACCTCGTGCGGTGGGTGTTTTTTTATTTTCGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTAtt                                                                                            >  1:648371/1‑150 (MQ=255)
                                                         gcgcGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAAt                                                                                         <  1:1164470/151‑1 (MQ=255)
                                                         gcgcGATAAGCTGAATATGGCGGTTTTTAAAGCCGCGCTTTTGCTGCTCGCCCGGCTGTTGACCTTCCATCATGAAAACTCGGGCGGTGGGTGTTTTTTTATCTACGCCGTGATGCGGGTGTAAGTTTGCAATTCCGTTTGTTGTATTaa                                                                                          >  3:142271/1‑150 (MQ=255)
                                                            cgATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTg                                                                                      <  2:899158/151‑1 (MQ=255)
                                                             gATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGt                                                                                     <  4:444811/151‑1 (MQ=255)
                                                               tAAGCTGAATATGGCGGTTTTTAAGGGCGCGCTTTTGATGGTCGCCGTGCTGTTGGCCCTCCCTCATGAAAACTCCTGCGGGGGTTGTTTTTTGATCTACGCCGTGGAGGGGGTGTTAGTTTGGAAATTCCTTTTTTGTATTAATtttttt                                                                                   >  2:1278225/1‑151 (MQ=255)
                                                                aaGCTGAATATGGCGGTTTTTTAAGTCGCCGGTTAGCTGCCCGCCGCTCTGTTGACCTTTCCTCATAAAAACTCTGGTGGGGGGTGTTTTTTTTTCTAATTAGGGATGAGTGTGGAAGGTTGCAATTCAGTTTTTTTTATTAATtttttt                                                                                   >  1:1711040/1‑150 (MQ=255)
                                                                  gCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACa                                                                                <  1:1597763/151‑1 (MQ=255)
                                                                   cTGAATATGGCGGTTTTTTAAGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCCTCATGAAAACTCGTGCGGGGGGTGTTTTTTTTTCTACGCAGTGATGCGTGTGGAAGTTTTCAATTTCGTTTGTTGTATTAATtttttt                                                                                   >  1:1103577/1‑147 (MQ=255)
                                                                   cTGAATATGGCGGTTTTTTAAGCCGCGCTTTAGCTGCTCGCCCTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGGGGGTGTTTTTTGATCTTCGCAGGGGTGCGTGTGTAAGTTTGCCATTCCGTTTTTTGTATTAATtttttttc                                                                                 >  1:1020989/1‑147 (MQ=255)
                                                                       atatGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCaaa                                                                           <  4:555768/151‑1 (MQ=255)
                                                                       atatGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCaaa                                                                           <  2:1348367/151‑1 (MQ=255)
                                                                       atatGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCaa                                                                            >  1:1709070/1‑150 (MQ=255)
                                                                        tatGGCGGTTTTTTAGGCCGCGCTTTAACTGCTCGCCGTTCTGTTGTCCTTCCATCATGAAAACTCGTTGGGGGGGTGTTTTTTTTTCTATGCAGTGATGCGGGGGTTAGGTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCaa                                                                            >  1:280962/1‑149 (MQ=255)
                                                                         atGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAaag                                                                          <  2:302531/150‑1 (MQ=255)
                                                                         atGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAaag                                                                          <  1:1086482/150‑1 (MQ=255)
                                                                         atGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAaag                                                                          <  2:1324271/150‑1 (MQ=255)
                                                                            gCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGt                                                                      >  2:1245397/1‑151 (MQ=255)
                                                                               gTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTg                                                                   >  1:395301/1‑151 (MQ=255)
                                                                                 ttttAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGa                                                                  <  2:706753/150‑1 (MQ=255)
                                                                                  tttAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAATTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAAt                                                                >  2:1716616/1‑151 (MQ=255)
                                                                                  tttAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAAt                                                                >  1:1501394/1‑151 (MQ=255)
                                                                                    tAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTg                                                              >  4:161634/1‑151 (MQ=255)
                                                                                    tAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTg                                                              >  1:906717/1‑151 (MQ=255)
                                                                                     aaGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGt                                                             >  2:129668/1‑151 (MQ=255)
                                                                                     aaGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGt                                                             >  2:504829/1‑151 (MQ=255)
                                                                                        gCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTAc                                                          >  3:292493/1‑151 (MQ=255)
                                                                                        gCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTAc                                                          <  2:1523901/151‑1 (MQ=255)
                                                                                        gCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTAc                                                          >  1:499976/1‑151 (MQ=255)
                                                                                           gcgcTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACaa                                                        >  2:386843/1‑150 (MQ=255)
                                                                                             gcTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACaaaaa                                                     <  1:915412/151‑1 (MQ=255)
                                                                                              cTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACaaaa                                                      >  1:888272/1‑149 (MQ=255)
                                                                                                 tAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACt                                                 >  2:1673607/1‑151 (MQ=255)
                                                                                                 tAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACt                                                 >  2:284473/1‑151 (MQ=255)
                                                                                                    ctgctCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTcc                                              >  1:1462181/1‑151 (MQ=255)
                                                                                                     tgctCGCCGTGCTGTTGACCTTCCATCATGAAACCTTGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCg                                             >  4:242147/1‑151 (MQ=255)
                                                                                                     tgctCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGGAAGTTTGCAATTCTGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAGGACTTCCg                                             >  2:636248/1‑151 (MQ=255)
                                                                                                      gctCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCg                                             <  1:330770/150‑1 (MQ=255)
                                                                                                      gctCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGACGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGt                                            >  2:1708308/1‑151 (MQ=255)
                                                                                                       ctCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTcc                                              <  2:1114828/148‑1 (MQ=255)
                                                                                                         cGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAg                                         <  3:34000/151‑1 (MQ=255)
                                                                                                             gTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCa                                     <  1:1561951/151‑1 (MQ=255)
                                                                                                              tGCTGTTGTCCTTCCATCATGAAAACTCGTGCGGTGGTTGTTTTTTGATTTACGCATTTATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCa                                     <  4:45509/150‑1 (MQ=255)
                                                                                                               gCTGTTGACCTTCCATCATGAAACCTCGTGCGGAGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCa                                     >  1:602463/1‑149 (MQ=255)
                                                                                                                cTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCa                                     >  1:1025442/1‑148 (MQ=255)
                                                                                                                  gTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGaat                                <  4:273327/151‑1 (MQ=255)
                                                                                                                     gACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGaataat                             >  2:1313614/1‑151 (MQ=255)
                                                                                                                          tCCATCATGAACCCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATGATGGTAt                        >  1:986945/1‑151 (MQ=255)
                                                                                                                           ccATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTAt                        <  1:826117/150‑1 (MQ=255)
                                                                                                                           ccATCATGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATg                       <  1:1239473/151‑1 (MQ=255)
                                                                                                                             atcatGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCg                     >  2:986373/1‑151 (MQ=255)
                                                                                                                               catGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCgg                    <  2:524941/150‑1 (MQ=255)
                                                                                                                               catGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCgg                    <  1:1587360/150‑1 (MQ=255)
                                                                                                                                atGAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCa                  <  4:312295/151‑1 (MQ=255)
                                                                                                                                  gAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAg                 <  2:1501556/150‑1 (MQ=255)
                                                                                                                                  gAAACCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGc                >  1:182015/1‑151 (MQ=255)
                                                                                                                                   aaaCCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCg               <  1:253853/151‑1 (MQ=255)
                                                                                                                                     aCCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCGaa             <  4:589390/151‑1 (MQ=255)
                                                                                                                                     aCCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCGaa             <  2:1391078/151‑1 (MQ=255)
                                                                                                                                     aCCTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCGaa             <  1:467358/151‑1 (MQ=255)
                                                                                                                                       cTCGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCGAATg           >  2:1185106/1‑151 (MQ=255)
                                                                                                                                         cGTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCGAATGc          <  4:38199/150‑1 (MQ=255)
                                                                                                                                          gTGCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCGAATGCAc        >  2:776248/1‑151 (MQ=255)
                                                                                                                                            gCGGTGGTTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCGAATGCAccc      <  3:454091/151‑1 (MQ=255)
                                                                                                                                                tggtTGTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCGAATGCACCCGCtt  >  1:1030986/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                 
CGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCGAATGCACCCGCTT  >  NC_000913/121422‑121717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: