Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,375,895 (G)6→7 coding (1027/1368 nt) dcuD → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,375,8891.G99.1% 416.8 / ‑4.3 111coding (1021/1368 nt)dcuDputative transporter
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (58/52);  total (58/53)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

CGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGAC  >  NC_000913/3375739‑3376033
                                                                                                                                                       |                                                                                                                                                 
cGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCa                                                                                                                                                    <  1:883897/151‑1 (MQ=255)
      cATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAgggggg                                                                                                                                            <  1:553217/151‑1 (MQ=255)
      cATAACTTGCGCGAAACAATGGACGACGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTCGCGAAAGGCTTAACGACGATTGGCACTGTCGCTGCGGTTATCAgggggg                                                                                                                                            >  1:808883/1‑151 (MQ=255)
       aTAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAggggggg                                                                                                                                           <  3:173140/151‑2 (MQ=255)
        tAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAggggg                                                                                                                                              >  1:14477/1‑148 (MQ=255)
          aCTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATAAgggggggcgg                                                                                                                                        >  1:1244197/1‑147 (MQ=255)
             tGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGCCGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGc                                                                                                                                      >  3:139208/1‑151 (MQ=255)
             tGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAgggggggcgga                                                                                                                                       <  1:674382/149‑6 (MQ=255)
                gcgAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCAtt                                                                                                                                   >  2:727296/1‑151 (MQ=255)
                  gAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATtct                                                                                                                                 <  1:1353087/151‑1 (MQ=255)
                    aaCAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATtctgg                                                                                                                               >  1:951113/1‑151 (MQ=255)
                    aaCAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATtctgg                                                                                                                               >  1:1134963/1‑151 (MQ=255)
                      cAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATtctggtc                                                                                                                             <  2:296528/151‑1 (MQ=255)
                       aaTGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATtctggtct                                                                                                                            <  1:1530757/151‑1 (MQ=255)
                        aTGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATtctggtctg                                                                                                                           >  2:518126/1‑151 (MQ=255)
                         tGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATtctggtctgg                                                                                                                          >  2:559575/1‑151 (MQ=255)
                          ggACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCATTTTGGCAACGTGGTAACGCTGGTGGGCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATtctgggctgg                                                                                                                          <  1:1018855/150‑1 (MQ=255)
                          ggACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCAGAGCATtctggtctgg                                                                                                                          <  1:1717611/150‑1 (MQ=255)
                           gACGATGTGCAGGCGTTTTTTGACGGCCTGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGc                                                                                                                        >  3:417500/1‑151 (MQ=255)
                            actaTGTGCAGGCGATTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCg                                                                                                                       >  2:1074588/4‑151 (MQ=255)
                            aCGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCg                                                                                                                       >  1:954726/1‑151 (MQ=255)
                            aCGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCg                                                                                                                       <  2:661856/151‑1 (MQ=255)
                                  tgCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTg                                                                                                                 >  3:105160/1‑151 (MQ=255)
                                   gCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTg                                                                                                                 <  2:790169/150‑1 (MQ=255)
                                     aGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCg                                                                                                              >  1:104566/1‑151 (MQ=255)
                                        cGTTTTTTGACGGCCTGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGtg                                                                                                            >  3:250470/1‑150 (MQ=255)
                                        cGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGtg                                                                                                            >  1:154887/1‑150 (MQ=255)
                                             tttGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGatt                                                                                                      >  4:112685/1‑151 (MQ=255)
                                              tttaCGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGatt                                                                                                      >  1:1207254/4‑150 (MQ=255)
                                              ttGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGatt                                                                                                      <  2:1713645/150‑1 (MQ=255)
                                               tGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGattat                                                                                                    >  2:708532/1‑151 (MQ=255)
                                                gACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGattatt                                                                                                   >  4:293666/1‑151 (MQ=255)
                                                gACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGattat                                                                                                    <  2:1604339/150‑1 (MQ=255)
                                                  cGGCATGGGTACTCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCTCTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGAttatta                                                                                                  <  4:408641/150‑1 (MQ=255)
                                                  cGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGAttatta                                                                                                  <  2:585325/150‑1 (MQ=255)
                                                  cGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATtattat                                                                                                 >  2:994077/1‑151 (MQ=255)
                                                    gCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATgg                                                                                               <  1:440814/151‑1 (MQ=255)
                                                       tGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTTGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGGTGGTTATTATGgcg                                                                                             >  1:884671/1‑150 (MQ=255)
                                                        gggTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGgcgc                                                                                            <  2:1594094/150‑1 (MQ=255)
                                                        gggTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCt                                                                                           <  3:391374/151‑1 (MQ=255)
                                                        gggTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCt                                                                                           >  3:574184/1‑151 (MQ=255)
                                                         ggTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTg                                                                                          >  4:42261/1‑151 (MQ=255)
                                                         ggTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTg                                                                                          <  3:480834/151‑1 (MQ=255)
                                                           tACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTTTTATGgcg                                                                                             >  1:1563432/1‑146 (MQ=255)
                                                               cAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCAtt                                                                                    <  1:851581/151‑1 (MQ=255)
                                                                aGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTg                                                                                   >  3:416664/1‑151 (MQ=255)
                                                                 gTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGc                                                                                  <  2:1450095/151‑1 (MQ=255)
                                                                  tttGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGcc                                                                                 >  2:37160/1‑151 (MQ=255)
                                                                    tGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCa                                                                                <  2:1349263/150‑1 (MQ=255)
                                                                        aaCGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTtgt                                                                           <  2:870134/151‑1 (MQ=255)
                                                                        aaCGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTtgt                                                                           >  2:548121/1‑151 (MQ=255)
                                                                          cGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGc                                                                         <  4:417480/151‑1 (MQ=255)
                                                                                aaCGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATtg                                                                    >  1:1139744/1‑150 (MQ=255)
                                                                                aaCGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATtg                                                                    <  3:68715/150‑1 (MQ=255)
                                                                                 aCGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATtgtg                                                                  <  1:45965/151‑1 (MQ=255)
                                                                                   gCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGAt                                                                <  3:343976/151‑1 (MQ=255)
                                                                                   gCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGAt                                                                >  2:864072/1‑151 (MQ=255)
                                                                                      ggtggtCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATgg                                                              <  2:1068746/150‑1 (MQ=255)
                                                                                       gtggtCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGc                                                            <  1:708427/151‑1 (MQ=255)
                                                                                        tggtCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGct                                                           >  2:473851/1‑151 (MQ=255)
                                                                                            cgcgGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTg                                                        <  1:320397/150‑1 (MQ=255)
                                                                                                ggTGAAATATTTGCGAAAGGCATAACGACGATTGGCACTGTAGATGCGGTTATCAGGGGGAGCGGAGCATTCTGGTCTGGGCGGTATTAGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGCTGGGCTCTgg                                                       <  1:1193027/147‑1 (MQ=255)
                                                                                                 gTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTTTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTTTGGCa                                                     >  2:1551552/1‑148 (MQ=255)
                                                                                                  tGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATgc                                                 >  1:1248674/1‑151 (MQ=255)
                                                                                                   gAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATgcg                                                <  4:433421/151‑1 (MQ=255)
                                                                                                    aaaTATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATgcgc                                               >  2:711941/1‑151 (MQ=255)
                                                                                                     aaTATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGcc                                              >  4:543381/1‑151 (MQ=255)
                                                                                                       tatTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCg                                             >  2:1609587/1‑150 (MQ=255)
                                                                                                       tatTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGt                                            >  2:560776/1‑151 (MQ=255)
                                                                                                        atTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTTGTCATTGCCATTTGGGCCATTGTGATGGGCTCTGGCAATGCGCCGtt                                           <  2:1135095/151‑1 (MQ=255)
                                                                                                        atTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGtt                                           <  1:973396/151‑1 (MQ=255)
                                                                                                         tttGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGttt                                          >  1:724440/1‑151 (MQ=255)
                                                                                                           tGCGTAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGCATTGGCGTGGTGATTATTATGGCGCTGGTCATTTCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTAt                                        >  1:1569404/1‑151 (MQ=255)
                                                                                                            gCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATg                                       <  2:753630/151‑1 (MQ=255)
                                                                                                               aaaGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGt                                      >  1:907649/1‑149 (MQ=255)
                                                                                                               aaaGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTc                                     >  1:1322208/1‑150 (MQ=255)
                                                                                                               aaaGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTc                                     >  1:973565/1‑150 (MQ=255)
                                                                                                               aaaGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCa                                    <  3:399155/151‑1 (MQ=255)
                                                                                                                  ggCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCAttt                                 <  4:202910/151‑1 (MQ=255)
                                                                                                                      tAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTga                               <  1:323517/149‑2 (MQ=255)
                                                                                                                      tAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGc                               <  2:1429240/149‑1 (MQ=255)
                                                                                                                        acgacgATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGt                           <  2:992225/151‑1 (MQ=255)
                                                                                                                        acgacgATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGt                           >  2:822738/1‑151 (MQ=255)
                                                                                                                        acgacgATTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGGCTGGGCGGGATTGGCGTGATGATTATTATGGCGCTGGGCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGt                           >  1:682907/1‑151 (MQ=255)
                                                                                                                         cgacgaTTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTc                          <  4:539210/151‑1 (MQ=255)
                                                                                                                             gaTTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTAt                      >  2:1290060/1‑151 (MQ=255)
                                                                                                                             gaTTGGCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTAt                      <  4:555749/151‑1 (MQ=255)
                                                                                                                                 ggCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTcc                   <  2:606860/150‑1 (MQ=255)
                                                                                                                                 ggCACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCg                  <  2:819590/151‑1 (MQ=255)
                                                                                                                                   cACTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGa                 >  2:1524094/1‑150 (MQ=255)
                                                                                                                                    aCTGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAat               <  1:601799/151‑1 (MQ=255)
                                                                                                                                      tGTCGATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATATCATTTGCCAGTCTTATTCCGAatat             >  1:357475/1‑151 (MQ=255)
                                                                                                                                          gATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGc          >  2:1372177/1‑150 (MQ=255)
                                                                                                                                          gATGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCa         <  4:466799/151‑1 (MQ=255)
                                                                                                                                            tGCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGc       >  1:959628/1‑151 (MQ=255)
                                                                                                                                             gCGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGcc      >  2:105898/1‑151 (MQ=255)
                                                                                                                                              cGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGcc      >  2:117436/1‑150 (MQ=255)
                                                                                                                                              cGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCg     >  2:194060/1‑151 (MQ=255)
                                                                                                                                              cGGTTATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCg     >  2:1183044/1‑151 (MQ=255)
                                                                                                                                                  tATCAGGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGAc  >  4:119127/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                 
CGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGAC  >  NC_000913/3375739‑3376033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: