Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,312,212 (T)5→6 coding (566/945 nt) truB ← tRNA pseudouridine synthase B: tRNA pseudouridine(55) synthase and putative tmRNA pseudouridine(342) synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,312,2071.T98.5% 531.8 / ‑1.8 132coding (571/945 nt)truBtRNA pseudouridine synthase B: tRNA pseudouridine(55) synthase and putative tmRNA pseudouridine(342) synthase
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base T (59/71);  total (60/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 138 total aligned reads displayed.

TAATGGATCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGAACTTCAATGCCC  >  NC_000913/3312058‑3312352
                                                                                                                                                      |                                                                                                                                                 
tAATGGATCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGt                                                                                                                                                  >  3:450814/1‑151 (MQ=255)
 aaTGGATCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGt                                                                                                                                                  <  1:392972/150‑1 (MQ=255)
   tGGATCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGtttt                                                                                                                                               >  1:640663/1‑151 (MQ=255)
    ggATCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGttttt                                                                                                                                              <  1:592186/151‑1 (MQ=255)
       tCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGttttttc                                                                                                                                            >  1:329365/1‑148 (MQ=255)
         aaGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGttttttcacc                                                                                                                                         >  1:768457/1‑146 (MQ=255)
           gTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCAccc                                                                                                                                         >  2:1326956/1‑150 (MQ=255)
           gTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCAccc                                                                                                                                         >  1:1419347/1‑150 (MQ=255)
            tAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCAccc                                                                                                                                         >  1:916325/1‑149 (MQ=255)
              aCTCCTCAGCTTAAAGATCCTTCTTATCATCGTGCTCAACAAGTTCACGCAGCTTCTCCATGTTCCCCATCCGTTCAACCGGATATTTACTTACCGCCAGACAGCGCGGATAACTAACATGCGCGCCACAGCATACTTTTTCACCCAGGtc                                                                                                                                    >  1:929825/1‑151 (MQ=255)
              aCTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGt                                                                                                                                     >  2:1285171/1‑151 (MQ=255)
              aCTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGt                                                                                                                                     <  2:92908/151‑1 (MQ=255)
               cTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGtc                                                                                                                                    >  1:1172362/1‑151 (MQ=255)
                 ccGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGtcat                                                                                                                                  <  2:1370277/151‑1 (MQ=255)
                     ggCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAat                                                                                                                              >  4:451826/1‑151 (MQ=255)
                     ggCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACAGGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAat                                                                                                                              <  1:153468/151‑1 (MQ=255)
                      gCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAatg                                                                                                                             >  2:436469/1‑151 (MQ=255)
                         ggAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCTCAGCCGAGTTTTTTCACCCAGGTCATCAatga                                                                                                                            <  2:631432/149‑1 (MQ=255)
                         ggAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAatgatg                                                                                                                          >  2:112556/1‑151 (MQ=255)
                          gAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATgg                                                                                                                         <  1:16075/151‑1 (MQ=255)
                           aaTATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGt                                                                                                                        <  1:249506/151‑1 (MQ=255)
                              atCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGc                                                                                                                      <  2:102845/150‑1 (MQ=255)
                               tCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCgg                                                                                                                    >  2:1250824/1‑151 (MQ=255)
                                   gctgTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGatat                                                                                                                <  1:337491/151‑1 (MQ=255)
                                   gctgTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGata                                                                                                                 <  2:457599/150‑1 (MQ=255)
                                   gctgTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGat                                                                                                                  <  2:497437/149‑1 (MQ=255)
                                    ctgTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGatat                                                                                                                >  2:1657824/1‑150 (MQ=255)
                                     tgTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGAtata                                                                                                               >  1:1071983/1‑150 (MQ=255)
                                      gTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATaa                                                                                                              <  2:694486/150‑1 (MQ=255)
                                      gTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATaa                                                                                                              <  3:481778/150‑1 (MQ=255)
                                      attCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACAGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGAtcta                                                                                                               >  1:1502850/2‑149 (MQ=255)
                                       ttCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAg                                                                                                             >  1:54594/1‑150 (MQ=255)
                                        tCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTg                                                                                                           >  1:688630/1‑151 (MQ=255)
                                         cAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTg                                                                                                           <  2:888988/150‑1 (MQ=255)
                                          aGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTg                                                                                                           <  2:265482/149‑1 (MQ=255)
                                             ttGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCttt                                                                                                      >  2:278694/1‑151 (MQ=255)
                                              tGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCttt                                                                                                      <  3:80996/150‑1 (MQ=255)
                                               gCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCtttt                                                                                                     <  1:1682622/150‑1 (MQ=255)
                                                 tCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTg                                                                                                    >  1:300687/1‑149 (MQ=255)
                                                   aacaaGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGc                                                                                                 <  1:882272/150‑1 (MQ=255)
                                                   aacaaGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCa                                                                                                <  2:1629020/151‑1 (MQ=255)
                                                      aaGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTg                                                                                             >  1:1163389/1‑151 (MQ=255)
                                                      aaGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTg                                                                                             >  2:1398080/1‑151 (MQ=255)
                                                       aGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGa                                                                                            <  1:739757/151‑1 (MQ=255)
                                                       aGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGa                                                                                            <  3:244549/151‑1 (MQ=255)
                                                          tCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAAtt                                                                                         >  2:1313812/1‑151 (MQ=255)
                                                           cACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAAtt                                                                                         <  1:235419/150‑1 (MQ=255)
                                                            aCGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCTCAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAAtt                                                                                         <  1:1677803/149‑1 (MQ=255)
                                                               cAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTtccag                                                                                    <  3:531594/151‑1 (MQ=255)
                                                                    gCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTtccagctcca                                                                               <  1:1659219/151‑1 (MQ=255)
                                                                    gCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTtccagctcc                                                                                <  2:558979/150‑1 (MQ=255)
                                                                    gCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTtccagctcc                                                                                <  4:174993/150‑1 (MQ=255)
                                                                    gCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTtccagctcc                                                                                >  4:317850/1‑150 (MQ=255)
                                                                      tCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTtccagctccagc                                                                             >  2:597652/1‑151 (MQ=255)
                                                                        cAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCcagctccagctc                                                                           <  2:807184/151‑1 (MQ=255)
                                                                        cAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGCGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCcagctccagctc                                                                           >  1:226168/1‑151 (MQ=255)
                                                                         aGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCa                                                                          <  2:814000/151‑1 (MQ=255)
                                                                          gggTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCAt                                                                         >  3:561244/1‑151 (MQ=255)
                                                                           ggTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCAtt                                                                        <  2:121307/151‑1 (MQ=255)
                                                                           ggTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCAtt                                                                        <  2:45510/151‑1 (MQ=255)
                                                                             tCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGc                                                                      >  2:856596/1‑151 (MQ=255)
                                                                              cACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGcc                                                                     <  2:754166/151‑1 (MQ=255)
                                                                                  aTCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTc                                                                  >  1:539716/1‑150 (MQ=255)
                                                                                    ccGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATg                                                               <  1:1641870/151‑1 (MQ=255)
                                                                                     cGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATgg                                                              >  1:1115671/1‑151 (MQ=255)
                                                                                     cGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATgg                                                              <  1:647531/151‑1 (MQ=255)
                                                                                          aaCCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAAt                                                         >  1:1105342/1‑151 (MQ=255)
                                                                                           aCCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATa                                                        <  1:1372106/151‑1 (MQ=255)
                                                                                            ccGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATaa                                                       <  1:485002/151‑1 (MQ=255)
                                                                                             cGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATaa                                                       >  2:111796/1‑150 (MQ=255)
                                                                                               gATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAAc                                                     >  2:846444/1‑150 (MQ=255)
                                                                                               gATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACa                                                    <  3:571726/151‑1 (MQ=255)
                                                                                                atatTTACTTACTGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAg                                                   <  3:322762/151‑1 (MQ=255)
                                                                                                atatTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAg                                                   <  1:1380001/151‑1 (MQ=255)
                                                                                                   tttACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCa                                                 <  2:905677/150‑1 (MQ=255)
                                                                                                    ttacttacCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCaa                                                <  1:1416768/150‑1 (MQ=255)
                                                                                                      acttacCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAAtt                                              <  1:811417/150‑1 (MQ=255)
                                                                                                       cttacCGCCAGACGGCGCAGGTAAATAACATGCTCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTc                                             >  1:763904/1‑150 (MQ=255)
                                                                                                        ttacCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCAt                                           >  4:584957/1‑151 (MQ=255)
                                                                                                          acCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATa                                          <  2:300731/150‑1 (MQ=255)
                                                                                                            cGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTc                                             <  2:375759/145‑1 (MQ=255)
                                                                                                             gCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACg                                      <  2:537533/151‑1 (MQ=255)
                                                                                                              ccAGACGGCGCAGGTAAATAACCTGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACgg                                     >  1:1317845/1‑151 (MQ=255)
                                                                                                                aGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTa                                   <  2:1559982/151‑1 (MQ=255)
                                                                                                                 gACGGCGCAGGTAAATACCATGCGCGCCACAGCCGAGTTTTTTCACCCAGGACATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGt                                    >  1:370909/1‑149 (MQ=255)
                                                                                                                  aCGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAAt                                 <  1:1560954/151‑1 (MQ=255)
                                                                                                                     gcgcAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCgg                              <  2:973994/151‑1 (MQ=255)
                                                                                                                      cgcAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGa                             <  4:180948/151‑1 (MQ=255)
                                                                                                                        cAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGAcg                           >  2:620470/1‑151 (MQ=255)
                                                                                                                        cAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGAcg                           <  2:946112/151‑1 (MQ=255)
                                                                                                                         aGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGAcgc                          >  4:235151/1‑151 (MQ=255)
                                                                                                                         aGGTAAATAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGAcgc                          >  1:1228697/1‑151 (MQ=255)
                                                                                                                                tAACATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCAc                   >  2:270976/1‑151 (MQ=255)
                                                                                                                                   cATGCGCGCCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCAcgcg                >  2:981187/1‑151 (MQ=255)
                                                                                                                                      gcgcgcCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGaa             <  4:409062/151‑1 (MQ=255)
                                                                                                                                         cgcCACAGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGAACtt          <  1:37430/151‑1 (MQ=255)
                                                                                                                                             acaGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGAACTTCAAt      <  2:1023072/151‑1 (MQ=255)
                                                                                                                                              caGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGAACTTCAAt      <  3:146215/150‑1 (MQ=255)
                                                                                                                                              caGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCAGGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGAACTTCAATg     <  1:972256/151‑1 (MQ=255)
                                                                                                                                               aGCCGAGTTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGAACTTCAATGc    >  1:402751/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                 
TAATGGATCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGAACTTCAATGCCC  >  NC_000913/3312058‑3312352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: