Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913354,2880TG46.5% 190.0 / 48.4 127V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (10/58);  new base G (59/0);  total (69/58)
Fisher's exact test for biased strand distribution p-value = 4.19e-26
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354141‑354437
                                                                                                                                                   |                                                                                                                                                      
ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGaa                                                                                                                                                     <  1:1108224/151‑1 (MQ=255)
 gggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGaaa                                                                                                                                                    <  3:369817/151‑1 (MQ=255)
 gggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGaaa                                                                                                                                                    <  3:543852/151‑1 (MQ=255)
  ggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAg                                                                                                                                                   <  3:286332/151‑1 (MQ=255)
     aTCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGa                                                                                                                                                  >  4:342929/1‑149 (MQ=255)
           gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAAATGCGCTg                                                                                                                                           >  3:123972/1‑150 (MQ=255)
              gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAg                                                                                                                                       <  1:1018212/151‑1 (MQ=255)
              gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAg                                                                                                                                       <  4:356338/151‑1 (MQ=255)
              gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCTGaa                                                                                                                                         >  2:1389395/1‑149 (MQ=255)
              gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGTGGAAGATga                                                                                                                                               >  2:1362759/1‑142 (MQ=255)
                  aCGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAggg                                                                                                                                     >  2:9138/1‑149 (MQ=255)
                   cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAggg                                                                                                                                     >  4:204830/1‑148 (MQ=255)
                     gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggt                                                                                                                                <  2:1462896/151‑1 (MQ=255)
                     gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTGGGGGGGGGAGATGGGCGGCAAGGGGAggg                                                                                                                                >  4:464143/1‑150 (MQ=255)
                       tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtg                                                                                                                               <  2:1610323/150‑1 (MQ=255)
                       tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCgggggg                                                                                                                              >  2:1394037/1‑151 (MQ=255)
                       tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGGAAAGGGCGCGGAAAggggcggg                                                                                                                                >  1:1271580/1‑144 (MQ=255)
                        aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCTGGAAGGGGAgggggg                                                                                                                             >  2:283431/1‑150 (MQ=255)
                          cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcg                                                                                                                            <  2:1081157/150‑1 (MQ=255)
                           aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGAAAGATGCGCTGGAAGGGCAgggggcgg                                                                                                                           >  3:42972/1‑150 (MQ=255)
                              ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtg                                                                                                                         <  3:593303/149‑1 (MQ=255)
                               tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAAAAGCGCTGAAAAGGCAGgtggggggggcg                                                                                                                      >  2:1295561/1‑151 (MQ=255)
                                  tctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGtt                                                                                                                    <  1:561820/150‑1 (MQ=255)
                                     cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTg                                                                                                                  <  2:1133530/149‑1 (MQ=255)
                                        gcgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCAtt                                                                                                            <  2:1729466/151‑1 (MQ=255)
                                          gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTcc                                                                                                          <  1:546153/151‑1 (MQ=255)
                                          gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCCGGAAGGGGAGGGGGCGGGGGGGGTTG‑TCATTcc                                                                                                          >  4:540619/1‑151 (MQ=255)
                                           cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCg                                                                                                         <  2:199116/151‑1 (MQ=255)
                                           cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAAAAGGGCTGGAAGGGGCGGGGGGGGGGGGGTTTG‑TCATTcc                                                                                                          >  1:286042/1‑150 (MQ=255)
                                             cTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAGGAAGCGCTGAGAGGGCCGGGGGCGGGGGAGGTTG‑ttagttcg                                                                                                         >  1:1228880/1‑142 (MQ=255)
                                              tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTG‑TCATTTCGaa                                                                                                       >  2:1095338/1‑150 (MQ=255)
                                               gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGaa                                                                                                       <  1:879488/149‑1 (MQ=255)
                                                 cGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTG‑TTATTCCGaaa                                                                                                      >  2:1058783/1‑148 (MQ=255)
                                                  gATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAagaga                                                                                                  <  2:992133/151‑1 (MQ=255)
                                                   aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAAAGCGCTGAAAAGGGAGGGGGGGGGGGGGGTTG‑TTATTCCGaaa                                                                                                      >  1:684985/1‑146 (MQ=255)
                                                       aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAGATGGGCTGAAAGGGGAGGGGGCGGGGGGGGTTG‑TCATTCCGAAagaga                                                                                                  >  2:47198/1‑146 (MQ=255)
                                                        aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACa                                                                                            <  2:13158/151‑1 (MQ=255)
                                                        aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACa                                                                                            <  1:941522/151‑1 (MQ=255)
                                                        aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACa                                                                                            <  1:1490271/151‑1 (MQ=255)
                                                          tAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAg                                                                                           <  1:865836/150‑1 (MQ=255)
                                                          tAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGGGGCGGAAGGGCAGGGGGCGGGGGGGGTTGTTCTTTCCGAAagggggggcag                                                                                            >  1:1023462/1‑141 (MQ=255)
                                                            aCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGGGCTGGAAGGGCAGGGGGGGGGGGGGGTTG‑TCCTTTCGAAAAAGAACGGCaagct                                                                                        >  1:436753/1‑147 (MQ=255)
                                                             tgTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGAAAGGTGCGCTGAAAGGGGAGGGGGGGGGGGCGTTTG‑TGCTTTCGGAAGGGAGCGGCAGTCTg                                                                                       >  1:1713997/2‑151 (MQ=255)
                                                             cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTg                                                                                       <  2:939767/151‑1 (MQ=255)
                                                             cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGGGGGGCTGAAAAGGGAGGGGGCGGGGGCGGTTG‑TGATTCCGAAAGAGAGGGAAAggc                                                                                         >  1:752493/1‑147 (MQ=255)
                                                               ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAGGCGCTGGAAGGGGAGGGGGGGGGGGGGGTTG‑TCCTTTCGGAAGAGAGGGACAGGCTg                                                                                       >  1:70282/1‑149 (MQ=255)
                                                                tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGa                                                                                     <  1:30984/150‑1 (MQ=255)
                                                                 gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCTGAAAGGGCAGGGGGGGGGGGGGGTTG‑TCATTTCGGAAGAGAGCGGCAGGCTgg                                                                                      >  2:898436/1‑148 (MQ=255)
                                                                 gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGCGGGGGGGGTTG‑TCATTCCGAAAGAGAGCGAAAGGCTgg                                                                                      >  2:1655616/1‑148 (MQ=255)
                                                                  ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGCGGGGGGGGTTG‑TCATTTCGAAAGAGAGCGACAGGCTgg                                                                                      >  2:1494036/1‑147 (MQ=255)
                                                                   cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAAGGCAGGGGGCGGGGGGGGTTG‑TCATTCCGAAAGAGAGCGGCAggc                                                                                         >  2:321930/1‑141 (MQ=255)
                                                                     ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCg                                                                               <  1:135603/151‑1 (MQ=255)
                                                                            gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgtg                                                                        <  1:729195/151‑1 (MQ=255)
                                                                            gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGCGTTTG‑TCCTTCCGAAAGAGAGCGACAGTCTGGAAGAACGTGAtgtg                                                                        >  1:1160508/1‑151 (MQ=255)
                                                                               ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcg                                                                     <  2:1389982/151‑1 (MQ=255)
                                                                               ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcg                                                                     <  1:779950/151‑1 (MQ=255)
                                                                               ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcg                                                                     <  1:733209/151‑1 (MQ=255)
                                                                                gggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcgc                                                                    <  2:400358/151‑1 (MQ=255)
                                                                                    cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGCGCTGAAAAGGCAGGGGGCGGGGGGGGTTG‑TCCTTCCGGAAGAGAGCGACAGTCTGGGAAATCGGGATGGGGGGCACt                                                                 >  1:391576/1‑150 (MQ=255)
                                                                                     gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCg                                                               <  1:1372907/151‑1 (MQ=255)
                                                                                        cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGGTTG‑TCATTCCGAAAGAGAGCGACAGGCTGGAAGATCGTGATGTGGCGCACTc                                                                >  2:25964/1‑147 (MQ=255)
                                                                                          agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTTGGGGGGAAGATGCGGTGAAAAGGGAGGGGGCGGGGGCGTTTG‑TCAATCCGAAAGAAAGCGACCGTCTGGAAGGTCGTGATGTGGAGCCCTCGCaa                                                            >  2:1643167/1‑149 (MQ=255)
                                                                                          agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGGAAGATGCGCCGAAAGGGCAGGTGGCGGGGGCGTTTG‑TCATTTCGCAAAAGAGAGACAGTCTGGAAAATCGTGATGGGGCGCACTCGc                                                              >  2:1271549/1‑147 (MQ=255)
                                                                                            aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAgaga                                                        <  2:948481/151‑1 (MQ=255)
                                                                                            aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAgaga                                                        <  2:962754/151‑1 (MQ=255)
                                                                                              tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGGAAGGGGAGGGGGGGGGGGCGGTTT‑TCCTTCCGAAAGAGAGCGACAGTCTGGGAGATAGTGATGTGGCGCACTCGCAAGAGaa                                                       >  4:204189/1‑150 (MQ=255)
                                                                                              tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGTGAAAGATACGCAGGAAGGGCAGGGGGGGGGGGCGGTTG‑TCATTTCGAAAGAGAGGGACAGTCTGGGAGATCGTGATGTGgcgccccggctagcg                                                         >  2:392286/1‑137 (MQ=255)
                                                                                               gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAgg                                                     <  2:1267351/151‑1 (MQ=255)
                                                                                               gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAg                                                      <  3:204848/150‑1 (MQ=255)
                                                                                                aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGc                                                    <  1:1579209/151‑1 (MQ=255)
                                                                                                 agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGGGGGGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCg                                                   >  2:348550/1‑151 (MQ=255)
                                                                                                  gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGa                                                  <  2:586957/151‑1 (MQ=255)
                                                                                                  gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGGGGGGGGGGTTG‑TCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGGGGTGTGGCGCACTCGCAAGAGAAGGCg                                                   >  2:1169468/1‑150 (MQ=255)
                                                                                                  gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAAAGCGCTGAAAGGGGCGGGGGCGGGGGGGGTTG‑GTATTCCCAAAGAGAACGACAGGCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAgg                                                     >  1:438869/1‑148 (MQ=255)
                                                                                                   agagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAt                                                 <  3:288065/151‑1 (MQ=255)
                                                                                                   agagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGAAGCGCCGGAAGGGCAGGGGGGGGGGGGGGTTG‑TCATTCCGAAAGAGAGAGAGCGTGTGGAAGAACGGGATGTGGCGCACTCGCAAGAGAAgg                                                     >  1:1649742/1‑147 (MQ=255)
                                                                                                   agagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGAAGCGCTTAAAGGGGAGGGGGGGGGGGCGGTTG‑TCATTTCGAAAGAGAGCGAAAATCTGGAAGATCGTGATGGGGGGCACTCGCAAAAGAAgg                                                     >  1:820172/1‑147 (MQ=255)
                                                                                                       tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGGTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTAt                                              >  2:400137/1‑150 (MQ=255)
                                                                                                       tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTG‑TCATTCCGAAAGAGAGCGACAGGCTGGGAGATCGGGATGTGGCGCACTCGCAAGAGAAGGGGAtt                                                >  2:775399/1‑148 (MQ=255)
                                                                                                        aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGAAAGGGCAGGTGGGGGGGGCGGTTG‑TCATTCCGGAAGAGAGCGGCAGTCTGGGAGAACGTGGTGTGGGGCCCTCCCAAGAGAAGGCGGATATgg                                            >  2:400533/1‑151 (MQ=255)
                                                                                                          ctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATgg                                            >  2:1474915/1‑149 (MQ=255)
                                                                                                            ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgcg                                          <  4:536024/149‑1 (MQ=255)
                                                                                                            ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAAATGCGCCGAAAGGGCCGGTGGCGGGGGGGGTTG‑TTTTTTCGAAAGAGGGCGACAGTCTGGGAGAACGTGATGTGGCGCACTCGCCAGAGGAAGGGATTTTGgcgc                                         >  2:1580470/1‑150 (MQ=255)
                                                                                                             cAGCCATCCGGGCGTTGCCGGAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGGGGGGGGGGTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgcgc                                         >  2:540657/1‑149 (MQ=255)
                                                                                                              aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCTCTCGCAAGAGAAGGCGATTATGGCGCtg                                       <  1:551939/150‑1 (MQ=255)
                                                                                                               gCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggt                                     <  2:1386003/151‑1 (MQ=255)
                                                                                                                ccATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGGAAGGGCAGGGGGGGGGGGCGGTTG‑TCATTTCGAAAGAGAGGGGCAGGCTGGGAGATCGGGATGTGGGGCACTCGCAAGAGAAGGGGGTTATGGCGCcgggg                                    >  1:439628/1‑149 (MQ=255)
                                                                                                                  aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGGGGGGGCGGTTG‑TCATTCCGAAAGAGAGCGACAGGCTGGAAGATCGGGATGTGGCGCACTCGCAAGAGAAGGGGATTATGGCGCtggtgg                                   >  2:1020091/1‑150 (MQ=255)
                                                                                                                    ccGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTTAAAGGGGAGGGGGGGGGGGGGGTTG‑TTATTTCGGAAGAGAGCGACAGTCTGGAAGATCGGGATGGGGCGCACTCGCAAGAGAAAGGGAGTATGGCGCTGGTGGAc                                 >  1:84338/1‑150 (MQ=255)
                                                                                                                        gggTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCa                            <  1:162323/149‑1 (MQ=255)
                                                                                                                             gCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAtt                        <  1:1445377/150‑1 (MQ=255)
                                                                                                                                 aaGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCaa                   <  2:1032249/151‑1 (MQ=255)
                                                                                                                                     ggCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACtt                <  1:1295411/150‑1 (MQ=255)
                                                                                                                                      gCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTg              <  1:791181/151‑1 (MQ=255)
                                                                                                                                        ggtggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgc            <  1:766792/151‑1 (MQ=255)
                                                                                                                                            gtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccgcc        <  2:685084/151‑1 (MQ=255)
                                                                                                                                            gtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccgc         >  4:363097/1‑150 (MQ=255)
                                                                                                                                              tGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGccc       <  4:175888/150‑1 (MQ=255)
                                                                                                                                                  gTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcgc  >  4:324443/1‑151 (MQ=255)
                                                                                                                                                   tGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcgc  >  1:1286044/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354141‑354437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑