Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913354,3180TG41.2% 233.6 / 11.3 114V538G (GTG→GGG) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (12/55);  new base G (47/0);  total (59/55)
Fisher's exact test for biased strand distribution p-value = 5.55e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

TTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAA  >  NC_000913/354171‑354466
                                                                                                                                                   |                                                                                                                                                     
 tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAAAAGCGCTGAAAAGGCAGgtggggggggcg                                                                                                                                                   >  2:1295561/1‑151 (MQ=255)
  aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGt                                                                                                                                                  <  2:1294872/151‑1 (MQ=255)
    tctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGtt                                                                                                                                                 <  1:561820/150‑1 (MQ=255)
       cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTg                                                                                                                                               <  2:1133530/149‑1 (MQ=255)
          gcgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCAtt                                                                                                                                         <  2:1729466/151‑1 (MQ=255)
            gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTcc                                                                                                                                       <  1:546153/151‑1 (MQ=255)
            gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCCGGAAGGGGAGGGGGCGGGGGGGGTTG‑TCATTcc                                                                                                                                       >  4:540619/1‑151 (MQ=255)
             cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCg                                                                                                                                      <  2:199116/151‑1 (MQ=255)
             cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAAAAGGGCTGGAAGGGGCGGGGGGGGGGGGGTTTG‑TCATTcc                                                                                                                                       >  1:286042/1‑150 (MQ=255)
              aCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGGTTG‑TTAtttc                                                                                                                                       >  2:1699602/1‑147 (MQ=255)
               cTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAGGAAGCGCTGAGAGGGCCGGGGGCGGGGGAGGTTG‑ttagttcg                                                                                                                                      >  1:1228880/1‑142 (MQ=255)
                tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTG‑TCATTTCGaa                                                                                                                                    >  2:1095338/1‑150 (MQ=255)
                   cGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTG‑TTATTCCGaaa                                                                                                                                   >  2:1058783/1‑148 (MQ=255)
                    gATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAagaga                                                                                                                               <  2:992133/151‑1 (MQ=255)
                     aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAAAGCGCTGAAAAGGGAGGGGGGGGGGGGGGTTG‑TTATTCCGaaa                                                                                                                                   >  1:684985/1‑146 (MQ=255)
                         aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGAc                                                                                                                          <  1:794181/151‑1 (MQ=255)
                         aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAGATGGGCTGAAAGGGGAGGGGGCGGGGGGGGTTG‑TCATTCCGAAagaga                                                                                                                               >  2:47198/1‑146 (MQ=255)
                          aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACa                                                                                                                         <  1:1490271/151‑1 (MQ=255)
                          aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACa                                                                                                                         <  2:13158/151‑1 (MQ=255)
                          aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACa                                                                                                                         <  1:941522/151‑1 (MQ=255)
                            tAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAg                                                                                                                        <  1:865836/150‑1 (MQ=255)
                            tAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGGGGCGGAAGGGCAGGGGGCGGGGGGGGTTGTTCTTTCCGAAagggggggcag                                                                                                                         >  1:1023462/1‑141 (MQ=255)
                              aCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGGGCTGGAAGGGCAGGGGGGGGGGGGGGTTG‑TCCTTTCGAAAAAGAACGGCaagct                                                                                                                     >  1:436753/1‑147 (MQ=255)
                               tgTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGAAAGGTGCGCTGAAAGGGGAGGGGGGGGGGGCGTTTG‑TGCTTTCGGAAGGGAGCGGCAGTCTg                                                                                                                    >  1:1713997/2‑151 (MQ=255)
                               cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTg                                                                                                                    <  2:939767/151‑1 (MQ=255)
                               cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGGGGGGCTGAAAAGGGAGGGGGCGGGGGCGGTTG‑TGATTCCGAAAGAGAGGGAAAggc                                                                                                                      >  1:752493/1‑147 (MQ=255)
                                gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGAAAGGGCCGGTGGGGGGGGCGGTTG‑TCCTTCCGAAAGAGAGCGACAGTCTgg                                                                                                                   >  2:1370358/1‑151 (MQ=255)
                                  tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGa                                                                                                                  <  1:30984/150‑1 (MQ=255)
                                   gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCTGAAAGGGCAGGGGGGGGGGGGGGTTG‑TCATTTCGGAAGAGAGCGGCAGGCTgg                                                                                                                   >  2:898436/1‑148 (MQ=255)
                                   gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGCGGGGGGGGTTG‑TCATTCCGAAAGAGAGCGAAAGGCTgg                                                                                                                   >  2:1655616/1‑148 (MQ=255)
                                    ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTG‑TCATTCCGAAAGAGAGCGACAGTCTgg                                                                                                                   >  4:256599/1‑147 (MQ=255)
                                    ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGCGGGGGGGGTTG‑TCATTTCGAAAGAGAGCGACAGGCTgg                                                                                                                   >  2:1494036/1‑147 (MQ=255)
                                     cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAAGGCAGGGGGCGGGGGGGGTTG‑TCATTCCGAAAGAGAGCGGCAggc                                                                                                                      >  2:321930/1‑141 (MQ=255)
                                       ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCg                                                                                                            <  1:135603/151‑1 (MQ=255)
                                              gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgtg                                                                                                     <  1:729195/151‑1 (MQ=255)
                                              gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGCGTTTG‑TCCTTCCGAAAGAGAGCGACAGTCTGGAAGAACGTGAtgtg                                                                                                     >  1:1160508/1‑151 (MQ=255)
                                                 ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcg                                                                                                  <  1:733209/151‑1 (MQ=255)
                                                 ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcg                                                                                                  <  2:1389982/151‑1 (MQ=255)
                                                      cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGCGCTGAAAAGGCAGGGGGCGGGGGGGGTTG‑TCCTTCCGGAAGAGAGCGACAGTCTGGGAAATCGGGATGGGGGGCACt                                                                                              >  1:391576/1‑150 (MQ=255)
                                                       gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCg                                                                                            <  1:1372907/151‑1 (MQ=255)
                                                        cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGCGGGGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTTGGAGATCGTGATGTGGGGCACTCGc                                                                                           >  2:1588728/1‑151 (MQ=255)
                                                        cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGACACGCGTAAAAGGGAAGAGGGGGGGGGGGTTG‑TGATTTAGAAAGAGAGGGATAGGCTTGAAGAGCGTGACGTGGGGCAccc                                                                                             >  2:746104/1‑147 (MQ=255)
                                                            agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTTGGGGGGAAGATGCGGTGAAAAGGGAGGGGGCGGGGGCGTTTG‑TCAATCCGAAAGAAAGCGACCGTCTGGAAGGTCGTGATGTGGAGCCCTCGCaa                                                                                         >  2:1643167/1‑149 (MQ=255)
                                                            agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGGAAGATGCGCCGAAAGGGCAGGTGGCGGGGGCGTTTG‑TCATTTCGCAAAAGAGAGACAGTCTGGAAAATCGTGATGGGGCGCACTCGc                                                                                           >  2:1271549/1‑147 (MQ=255)
                                                            agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAaga                                                                                       <  2:526894/151‑1 (MQ=255)
                                                            agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAGATGCGCTGAAAAGGCAGGGGGCGGGGGCGGTTG‑TCCTTCCGAAAGAGAGCGGCCGTCTGGAAGATCGTGATGTGGCGCACTccc                                                                                           >  4:452536/1‑145 (MQ=255)
                                                              aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAgaga                                                                                     <  2:948481/151‑1 (MQ=255)
                                                              aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAgaga                                                                                     <  2:962754/151‑1 (MQ=255)
                                                                tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGGAAGGGGAGGGGGGGGGGGCGGTTT‑TCCTTCCGAAAGAGAGCGACAGTCTGGGAGATAGTGATGTGGCGCACTCGCAAGAGaa                                                                                    >  4:204189/1‑150 (MQ=255)
                                                                tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGTGAAAGATACGCAGGAAGGGCAGGGGGGGGGGGCGGTTG‑TCATTTCGAAAGAGAGGGACAGTCTGGGAGATCGTGATGTGgcgccccggctagcg                                                                                      >  2:392286/1‑137 (MQ=255)
                                                                 gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAgg                                                                                  <  2:1267351/151‑1 (MQ=255)
                                                                 gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAg                                                                                   <  3:204848/150‑1 (MQ=255)
                                                                 gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAAGGGAGGGGGGGGGGGCGGTTG‑TCATTCCGAAAGAGAGCGACAGGCTGGGAGATCGGGATGTGGCGCACTCGCAAGAGAAgg                                                                                  >  2:1420432/1‑151 (MQ=255)
                                                                  aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGc                                                                                 <  1:1579209/151‑1 (MQ=255)
                                                                   agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGGAAGGGCAGGGGGCGGGGGCGTTTG‑TCATTCCGAAAGAGAGGGACAGTCTGGAAGATCGTGGTGTGGGGCACTCGCAAGAGAAGGCg                                                                                >  2:1050393/1‑151 (MQ=255)
                                                                   agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGGGGGGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCg                                                                                >  2:348550/1‑151 (MQ=255)
                                                                    gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGa                                                                               <  1:620786/151‑1 (MQ=255)
                                                                    gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGa                                                                               <  2:586957/151‑1 (MQ=255)
                                                                    gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGGGGGGGGGGTTG‑TCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGGGGTGTGGCGCACTCGCAAGAGAAGGCg                                                                                >  2:1169468/1‑150 (MQ=255)
                                                                    gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAAAGCGCTGAAAGGGGCGGGGGCGGGGGGGGTTG‑GTATTCCCAAAGAGAACGACAGGCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAgg                                                                                  >  1:438869/1‑148 (MQ=255)
                                                                     agagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAt                                                                              <  3:288065/151‑1 (MQ=255)
                                                                     agagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGAAGCGCCGGAAGGGCAGGGGGGGGGGGGGGTTG‑TCATTCCGAAAGAGAGAGAGCGTGTGGAAGAACGGGATGTGGCGCACTCGCAAGAGAAgg                                                                                  >  1:1649742/1‑147 (MQ=255)
                                                                     agagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGAAGCGCTTAAAGGGGAGGGGGGGGGGGCGGTTG‑TCATTTCGAAAGAGAGCGAAAATCTGGAAGATCGTGATGGGGGGCACTCGCAAAAGAAgg                                                                                  >  1:820172/1‑147 (MQ=255)
                                                                         tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGGTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTAt                                                                           >  2:400137/1‑150 (MQ=255)
                                                                         tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTG‑TCATTCCGAAAGAGAGCGACAGGCTGGGAGATCGGGATGTGGCGCACTCGCAAGAGAAGGGGAtt                                                                             >  2:775399/1‑148 (MQ=255)
                                                                          aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGAAAGGGCAGGTGGGGGGGGCGGTTG‑TCATTCCGGAAGAGAGCGGCAGTCTGGGAGAACGTGGTGTGGGGCCCTCCCAAGAGAAGGCGGATATgg                                                                         >  2:400533/1‑151 (MQ=255)
                                                                             tcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGGTTG‑TCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGGGGTGTGGCGCACTCGCAAGAGAAGGCGGTTATGgcg                                                                       >  2:393556/1‑150 (MQ=255)
                                                                              ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgcg                                                                       <  4:536024/149‑1 (MQ=255)
                                                                              ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAAATGCGCCGAAAGGGCCGGTGGCGGGGGGGGTTG‑TTTTTTCGAAAGAGGGCGACAGTCTGGGAGAACGTGATGTGGCGCACTCGCCAGAGGAAGGGATTTTGgcgc                                                                      >  2:1580470/1‑150 (MQ=255)
                                                                              ccAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGGTGGGGGGGAAAATACGCTGGAAGGGCCGGTGGGGGGGGGGGTTG‑TACTTCCGACAGAAGGCGACCGGCTTGCAGATCGAGAGGGGGGGCACTCGCAAGAGAAAGCGGTTATGgcg                                                                       >  1:1310398/1‑149 (MQ=255)
                                                                               cAGCCATCCGGGCGTTGCCGGAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGGGGGGGGGGTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgcgc                                                                      >  2:540657/1‑149 (MQ=255)
                                                                                aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCTCTCGCAAGAGAAGGCGATTATGGCGCtg                                                                    <  1:551939/150‑1 (MQ=255)
                                                                                 gCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggt                                                                  <  2:1386003/151‑1 (MQ=255)
                                                                                  ccATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGGAAGGGCAGGGGGGGGGGGCGGTTG‑TCATTTCGAAAGAGAGGGGCAGGCTGGGAGATCGGGATGTGGGGCACTCGCAAGAGAAGGGGGTTATGGCGCcgggg                                                                 >  1:439628/1‑149 (MQ=255)
                                                                                    aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGGGGGGGCGGTTG‑TCATTCCGAAAGAGAGCGACAGGCTGGAAGATCGGGATGTGGCGCACTCGCAAGAGAAGGGGATTATGGCGCtggtgg                                                                >  2:1020091/1‑150 (MQ=255)
                                                                                      ccGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTTAAAGGGGAGGGGGGGGGGGGGGTTG‑TTATTTCGGAAGAGAGCGACAGTCTGGAAGATCGGGATGGGGCGCACTCGCAAGAGAAAGGGAGTATGGCGCTGGTGGAc                                                              >  1:84338/1‑150 (MQ=255)
                                                                                            gTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTG‑TCATTCCGAAAGAGAGCGGCAGGCTTGAAGATCGTGGTGTGGGGCACCCGCCAGAGAAGGCGGTTATGGGGCGGGGGGGCAGcc                                                          >  1:1186800/1‑148 (MQ=255)
                                                                                               gCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAtt                                                     <  1:1445377/150‑1 (MQ=255)
                                                                                                   aaGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCaa                                                <  1:1655452/151‑1 (MQ=255)
                                                                                                       ggCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACtt                                             <  1:1295411/150‑1 (MQ=255)
                                                                                                        gCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTg                                           >  2:941327/1‑151 (MQ=255)
                                                                                                        gCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTg                                           <  1:791181/151‑1 (MQ=255)
                                                                                                          ggtggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgc                                         <  1:766792/151‑1 (MQ=255)
                                                                                                              gtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccgcc                                     <  2:685084/151‑1 (MQ=255)
                                                                                                              gtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccgc                                      <  3:363116/150‑1 (MQ=255)
                                                                                                                tGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGccc                                    <  4:175888/150‑1 (MQ=255)
                                                                                                                    gTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcgc                               >  4:324443/1‑151 (MQ=255)
                                                                                                                      gAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCAc                             <  2:1023591/151‑1 (MQ=255)
                                                                                                                       aaaGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCAc                             <  1:9136/150‑1 (MQ=255)
                                                                                                                         aGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTc                          <  1:1480112/151‑1 (MQ=255)
                                                                                                                          gATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCt                         <  3:426204/151‑1 (MQ=255)
                                                                                                                          gATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCt                         <  4:218928/151‑1 (MQ=255)
                                                                                                                           aTGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTg                        >  1:1239662/1‑151 (MQ=255)
                                                                                                                                    aaGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGt                  >  2:1395316/1‑148 (MQ=255)
                                                                                                                                      gggCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGc             >  1:1620933/1‑151 (MQ=255)
                                                                                                                                          aGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAAtt         <  1:1366924/151‑1 (MQ=255)
                                                                                                                                           ggtggcggtggcGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTg        <  3:464160/151‑1 (MQ=255)
                                                                                                                                              ggcggtggcgTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGcc      >  4:410589/1‑150 (MQ=255)
                                                                                                                                              ggcggtggcgTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGcc      <  2:1532830/150‑1 (MQ=255)
                                                                                                                                                 ggtggcgTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCaaa   >  1:71051/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAA  >  NC_000913/354171‑354466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑