Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913987,4940TG40.0% 209.0 / 26.4 115intergenic (‑512/+91)ompF/asnSouter membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (14/55);  new base G (46/0);  total (60/55)
Fisher's exact test for biased strand distribution p-value = 6.15e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

TCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCA  >  NC_000913/987346‑987643
                                                                                                                                                    |                                                                                                                                                      
tcttTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGtt                                                                                                                                                       <  2:1199491/150‑1 (MQ=255)
tcttTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTg                                                                                                                                                      >  3:248334/1‑151 (MQ=255)
    tCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAATACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCaa                                                                                                                                                   <  2:820558/150‑1 (MQ=255)
       gACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAg                                                                                                                                               <  1:1124391/151‑1 (MQ=255)
        aCATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGTaaa                                                                                                                                                  >  3:230235/1‑147 (MQ=255)
         cATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGTGTCGGaaaaaa                                                                                                                                               >  4:39303/1‑146 (MQ=255)
          aTCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGAtt                                                                                                                                            <  1:821438/151‑1 (MQ=255)
          aTCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGaaaaaaa                                                                                                                                              >  2:399336/1‑145 (MQ=255)
           tCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAATaaa                                                                                                                                              >  2:712238/1‑146 (MQ=255)
              aGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCtt                                                                                                                                        <  1:311622/151‑1 (MQ=255)
               gAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAGaattattt                                                                                                                                       >  1:1693771/1‑144 (MQ=255)
               gAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCaaaaaaaattctt                                                                                                                                        >  2:53557/1‑140 (MQ=255)
               gAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAATAAATTGttt                                                                                                                                        >  1:1398408/1‑150 (MQ=255)
                aaTGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGa                                                                                                                                      <  1:938260/151‑1 (MQ=255)
                aaTGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTTCAAATAGAATTCttt                                                                                                                                       >  2:457490/1‑149 (MQ=255)
                 aTGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAACAGAATGCttttt                                                                                                                                     >  2:1147485/1‑148 (MQ=255)
                  tGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGTAAAAAGaattttttact                                                                                                                                    >  2:1647668/1‑141 (MQ=255)
                  tGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCaaaaaaaattttttatt                                                                                                                                    >  1:1231277/1‑137 (MQ=255)
                    ccAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCCAATAAAATGCTTGACtt                                                                                                                                   >  4:218624/1‑150 (MQ=255)
                       aaaaaTAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTaaca                                                                                                                               <  2:1200250/151‑1 (MQ=255)
                        aaaaTAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTGGCAAATAAAATGCttttc                                                                                                                                     >  2:21565/1‑141 (MQ=255)
                        aaaaTAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAATAAATTGCtttgttttaaa                                                                                                                               >  3:270186/1‑141 (MQ=255)
                         aaaTAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTTATTTTCTTaacaa                                                                                                                              >  2:1500056/1‑150 (MQ=255)
                          aaTAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTaacaaac                                                                                                                            <  3:18279/151‑1 (MQ=255)
                          aaTAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGTAAATAAAtttgttttctttaaaaa                                                                                                                             >  2:1385361/1‑135 (MQ=255)
                           aTAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTaacaaaca                                                                                                                           <  1:1445006/151‑1 (MQ=255)
                           aTAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAAAATGCTTTGCTTtacaaa                                                                                                                             >  2:1346454/1‑149 (MQ=255)
                             aGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAg                                                                                                                          <  4:180098/150‑1 (MQ=255)
                              gTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGAt                                                                                                                        <  3:185220/151‑1 (MQ=255)
                                aTGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATaa                                                                                                                      <  3:202186/151‑1 (MQ=255)
                                aTGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATa                                                                                                                       <  1:338639/150‑1 (MQ=255)
                                  gAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACt                                                                                                                    <  4:206632/151‑1 (MQ=255)
                                   aaaaTTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAGAATTATTTATTTaacaaaaaaaaa                                                                                                                       >  1:713916/1‑141 (MQ=255)
                                    aaaTTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTg                                                                                                                  <  1:1604992/151‑1 (MQ=255)
                                     aaTTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGa                                                                                                                 <  3:475500/151‑1 (MQ=255)
                                     aaTTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTGGTAAAAAAATTGGTTGTCTTaaaaaaaaaaaaa                                                                                                                      >  1:979329/1‑139 (MQ=255)
                                       ttGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAAATTGCTTTACTTTAAAAAAAAAAAAATTGAc                                                                                                                >  2:1181005/1‑150 (MQ=255)
                                        tgtgCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAGATTTCTTTACTTTAAAAACAGATAAAtttcc                                                                                                                >  2:1643136/1‑146 (MQ=255)
                                         gtgCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAg                                                                                                              <  2:1695109/150‑1 (MQ=255)
                                         gtgCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAg                                                                                                              <  2:1604356/150‑1 (MQ=255)
                                         gtgCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAg                                                                                                              <  1:1496779/150‑1 (MQ=255)
                                         gtgCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAAATTGTTTGACTTtacaaaaaaaaaaatt                                                                                                                   >  3:217846/1‑135 (MQ=255)
                                           gCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGaaa                                                                                                          <  4:435457/151‑1 (MQ=255)
                                             tATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAGAATTCTTGACTTTACAAAAAAATAAATTGTAAGaaaa                                                                                                         >  2:1035094/1‑150 (MQ=255)
                                              aTTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGaaaa                                                                                                         <  2:472928/149‑1 (MQ=255)
                                                 tgCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAAATTTCTTGTATTTAAAAAAAGAAAACTTTACAGaaaaaaaa                                                                                                     >  1:1191896/2‑146 (MQ=255)
                                                   cTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTTCAAAAAAATTTATTTGCTTAACAAACAAATAAATTTACCGAAAAGAaaaa                                                                                                   >  2:1532760/1‑150 (MQ=255)
                                                    tAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAGATTTCTTTTCTTAAAAAAAAGAAAAATTGACAGAaaaaaaaaaa                                                                                                  >  2:663521/1‑150 (MQ=255)
                                                     aaGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATaaaaaa                                                                                                <  1:637343/151‑1 (MQ=255)
                                                     aaGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATaaaaaa                                                                                                <  1:230175/151‑1 (MQ=255)
                                                     aaGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATaaaaa                                                                                                 <  4:456202/150‑1 (MQ=255)
                                                       gAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAag                                                                                              <  3:377505/151‑1 (MQ=255)
                                                       gAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAag                                                                                              <  1:1073795/151‑1 (MQ=255)
                                                          aaaaCTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAGATTGGTTGACTTTACAAAAAAAAAAATTGACAGaaaaaaaaaaaaaaaa                                                                                             >  2:1355623/1‑147 (MQ=255)
                                                           aaaCTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGGAAAAAAATTATTTTCATAAAAAAAAGATAACATTATAGAAAAAATaaaaaaaaa                                                                                             >  1:1112681/1‑146 (MQ=255)
                                                              cTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGTCAATCAATTGTTTTACTTAACAAACAAATAACTTTTAAGAAAAACAAAAAAAaaaga                                                                                           >  3:505313/1‑146 (MQ=255)
                                                               tAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAAAATTTTTGGCTTTAAAAACAAATAAAATGACAGaaaaaaaaaaaaaaaa                                                                                             >  1:627352/1‑142 (MQ=255)
                                                                aaTCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCg                                                                                      <  3:549247/150‑1 (MQ=255)
                                                                aaTCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAAATTTTTTGACTTTACAAAAAAAAAAATTTAAAGaaaaaaaaaaaaaaaa                                                                                             >  2:1543395/1‑141 (MQ=255)
                                                                 aTCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCggg                                                                                    <  1:629742/151‑1 (MQ=255)
                                                                 aTCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAAAATTGTTGTCTTTAAAAAAAAAAAACTTTACAAAAAAAAAAAAAAAAAAAGCAGCggg                                                                                    >  2:625464/1‑151 (MQ=255)
                                                                   ccGCAATAATAATGCGGATTAGTTTTTTCGCGTAAATGTTGCAATGTAATACTCCGTAACAGGATGAACGTTATGTCGGGGTTGCAAATAGATAGCTTGACTTAACAAACAGATAAATTGACATACAAGATAAAAAAAGTGCCAGCGGgct                                                                                  <  4:526507/151‑2 (MQ=255)
                                                                   ccGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCCAAAAGATTGCTTTTTTTAAAAAAAACAAAAATTGACAGAAAAGAAAAAAAAAAAAACAGCggg                                                                                    >  2:440477/1‑149 (MQ=255)
                                                                    cGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCGAAAAGAATTCTTTTTTTTACAAAAAAATAAAATTaaaaaaaaaaaaaaaaaaaaaacaacggg                                                                                    >  1:606731/1‑137 (MQ=255)
                                                                     gCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAAATTTTTTTTCTTTAAAAAAAAAAAAATTGACAGaaaaaaaaaaaaaaaaaacaacgggggc                                                                                 >  3:363595/1‑136 (MQ=255)
                                                                          aaGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGctc                                                                           <  1:440405/151‑1 (MQ=255)
                                                                           aGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGctct                                                                          <  2:840486/151‑1 (MQ=255)
                                                                            gAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGGAAAAAAAATTTTTTACTTTAAAAAAAAAAAAATTTAAAGAAAAAAAAAAAAAAAAACCAGCGGGCGCCGGGTCtt                                                                         >  1:1651774/1‑151 (MQ=255)
                                                                              aTGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGa                                                                       <  3:595625/151‑1 (MQ=255)
                                                                                gCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAg                                                                     <  1:399279/151‑1 (MQ=255)
                                                                                  tgATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAAATTTTTTTACTTTAAAAAAAGATAAATTGAAAGAAAAAAAAAAAAAAAAAACAGCGGGGGGCGGCTCTTTAAGa                                                                    >  1:582379/2‑150 (MQ=255)
                                                                                  ggATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGAc                                                                   <  1:1551913/151‑1 (MQ=255)
                                                                                   gATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACg                                                                  <  1:1678749/151‑1 (MQ=255)
                                                                                    aTTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGc                                                                 <  3:410586/151‑1 (MQ=255)
                                                                                    aTTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAAAATTTTTTACTTTACAAACAGAAAAATTGACAAAAAAAATAAAAAAAAAGCCAGCGGGCGCTGGCTCTTGaa                                                                      >  2:1126878/1‑146 (MQ=255)
                                                                                      tAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCt                                                                <  4:435692/150‑1 (MQ=255)
                                                                                      tAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGTGAAAAAATTTCTTTTCTTAACAAAAAAATAAATTGACAGAAAAAAAAAAAAAAAAGACAGCGGGGGGGGGGCCTTGAAGACGcc                                                                >  2:799246/1‑149 (MQ=255)
                                                                                      tAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAAAATTTTTGATTTTAAAAAAAAAAAAATTTTCAGAAAAAAAAAAAAAAAAAGCCACGGGCGCCgggcccttaaaaagct                                                                >  4:415115/1‑136 (MQ=255)
                                                                                       aGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTTCAAAAAAATTTCTTTGCTTTACAAAAAAAAAAACTGAAAGAAAAAAAAAAAAAAAAACCAACggggggcgggccttg                                                                        >  2:1280683/1‑129 (MQ=255)
                                                                                             tGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAGATTGTTTTACTTTACAAAAAAAAAAATTTACAGAAAAAATAAAAAAAAAACCAGCGGGCGCTGGCTCTTGAAGACGCTaaa                                                             >  2:786175/1‑146 (MQ=255)
                                                                                               cgcgTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAg                                                      <  1:457415/151‑1 (MQ=255)
                                                                                                  gTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGGAAAAAAATGTTTTACTTTAAAAAAAAAAAAATTTACAAAAAAAAAAAAAAAAAAACCAGCGGGGGCTGGGTCTTGAAGAAGCTAAAATAAAAGCTg                                                   >  2:450625/1‑151 (MQ=255)
                                                                                                     aaTGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCg                                                <  1:812866/151‑1 (MQ=255)
                                                                                                      aTGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAAAAAAATGGTTGAATTTACAAAAAAAAAAATTTACCGAAAAAAAAAAAAAAAAAACAACGGGGGCCGGGTCTTGAAAAAGGTAAATTAAAAACTgggg                                                >  3:463482/1‑148 (MQ=255)
                                                                                                             aaTGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAAAATGCTTGTCTTTACAAAAAAAAAAATTTAAAGAAAAGAAAAAAAAAAAACCAGCGGGCGCTGGGTCTTGTAGAAGCTCAATTATaaacctgcggttcac                                          >  2:1392234/1‑136 (MQ=255)
                                                                                                                  aaTATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTa                                    <  1:910908/150‑1 (MQ=255)
                                                                                                                  aaTATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAAATTTCTTTTCTTTAAAAAAAAAAAAATTTACAGAAAAAAAAAAAAAAAAAACAGCGGGGGGCGGGCCTTTAAAAAGCCAAAATAAAAGCTGGCGGTACCCGGa                                       >  2:759434/1‑147 (MQ=255)
                                                                                                                   atatTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAAATTTGTTGACTTAAAAAAAAAAAAAATTTACAGAAAAAAAAAAAAAAAAAACAGCGGGGGCTGGGCCTTGAAGACGCCAAATTAAAAGCAGGGGGTTCGCGGAg                                      >  2:653659/1‑147 (MQ=255)
                                                                                                                          gTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAAAATTTTTTACTTAAAAAAAAGAAAAAATGTCAGAAAAAAAAAAAAAAAAAGCAGCGGGCGGTGGGTCTTTAAGACGCCAAAATAAAAAATAGCGGTTCGCGGAGGACGAgggg                             >  1:602393/1‑148 (MQ=255)
                                                                                                                           tAACAGGATGATCGTTATGGCGGGGGGGGAAATAAAATTCTTTTTTTTACAAAAAAATAAAATTTAAAAAAAAAAAAAAAAAAAAACAGCGGGGGCCGGGGCTTGTAGAAGCCAAAATATAAGATGGCGGTACGCGGAggaaggggg                              >  1:1042893/1‑139 (MQ=255)
                                                                                                                           aaaCAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACg                          <  1:341643/150‑1 (MQ=255)
                                                                                                                                  atgatCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAatca                    <  1:886940/150‑1 (MQ=255)
                                                                                                                                       cGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAatcacatca               <  1:1249488/150‑1 (MQ=255)
                                                                                                                                            tGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCacgtacg         >  2:989001/1‑151 (MQ=255)
                                                                                                                                             ggCGGGGTTGCAAATAGATTGGTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAACGAACCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGGTACGCGGAGTACGCGGGAACGGAATCACATCacgtacgt        >  1:1476557/1‑151 (MQ=255)
                                                                                                                                              gCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGtt       <  1:478195/151‑1 (MQ=255)
                                                                                                                                              gCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGtt       <  2:1398562/151‑1 (MQ=255)
                                                                                                                                                 gggTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTg    <  1:1497826/151‑1 (MQ=255)
                                                                                                                                                 gggTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTg    <  2:486723/151‑1 (MQ=255)
                                                                                                                                                   gTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCa  <  2:503847/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCA  >  NC_000913/987346‑987643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: