Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,397,8300TC39.4% 188.2 / 39.1 133I265V (ATT→GTT) uspEstress‑induced protein
Reads supporting (aligned to +/- strand):  ref base T (53/27);  new base C (0/52);  total (53/80)
Fisher's exact test for biased strand distribution p-value = 5.87e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

CTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACG  >  NC_000913/1397680‑1397978
                                                                                                                                                       |                                                                                                                                                    
cttcttCGTCATGCAGTTCAACGGGTGTCTGATACTGCTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAAt                                                                                                                                                      >  1:75073/1‑151 (MQ=255)
  tcttcGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATcc                                                                                                                                                    >  1:448142/1‑151 (MQ=255)
     tcGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCg                                                                                                                                                 >  2:424672/1‑151 (MQ=255)
      cGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCg                                                                                                                                                 <  1:965296/150‑1 (MQ=255)
      cGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGc                                                                                                                                                <  1:937145/151‑1 (MQ=255)
       gTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGcc                                                                                                                                               >  2:1736390/1‑151 (MQ=255)
       gTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGcc                                                                                                                                               >  2:916058/1‑151 (MQ=255)
         cATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGATGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATcccctc                                                                                                                                                <  1:1398472/148‑3 (MQ=255)
         cATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTg                                                                                                                                             >  2:1589239/1‑151 (MQ=255)
             cAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTa                                                                                                                                           >  1:743911/1‑149 (MQ=255)
             cAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTa                                                                                                                                           >  2:144553/1‑149 (MQ=255)
                  cAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTc                                                                                                                                    >  2:934590/1‑151 (MQ=255)
                         tgtCTGATACTGTCTAGTTTTAATCACCAGCAGGCCGCAGCAAAAATGCTCAATCCCCTGTCCCCCCGTTTTGCCGAGAAATGCTGCTGAAATCCCGGTGCGCCCTACCGTCCCCAGAACCCTAACCCCCGCCTGTAAGTGCTCCGCCaa                                                                                                                              <  1:1677050/150‑1 (MQ=255)
                          gtCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCCCCTGTCCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGACCCAAAACCCCCGCCTGTAAGTGCTCCGCCaaa                                                                                                                             <  2:450164/150‑1 (MQ=255)
                           tCTGATACTGGTCAGTTTAAATGCCCAGCGGGTCGCAGCGAAGATGATCAATCCCCTGTTCCGCCGTGTTGCCGAGAAATGCTGCTGAAATACCGGTGCGTCCTACCGTCCCCAAAACCACAACCCCCGCCTGTAAGTGCTCCGCCaaa                                                                                                                             <  1:871781/149‑1 (MQ=255)
                             tGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATc                                                                                                                           >  2:513828/1‑149 (MQ=255)
                               atcgtGGTGATGTTAAATGCCCAGCGGTCCCCAGCAAAGTTGATCAACCCCCTTTCCCGCCGTGTTCCCGAAAAATGCTTCTGAAATCCGGGTGCGCCCAACCGTGCCCAAAACCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCa                                                                                                                          <  1:1086625/144‑1 (MQ=255)
                               aTACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAgg                                                                                                                        >  2:925390/1‑150 (MQ=255)
                                 aCTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAAt                                                                                                                     >  4:472104/1‑151 (MQ=255)
                                  cgggTCAGGTTTAAGGACCAGAAGGTCGCGGAAAATGTGAACAATCACGTGTTCCGCATTGTTGCGGAGAAAGGCTGCGGAAATCCCGGTGCGCCCTACCGGGCCGAAAACCCCACCCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAAt                                                                                                                     <  1:403177/148‑1 (MQ=255)
                                  cTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATc                                                                                                                    >  1:654491/1‑151 (MQ=255)
                                   tGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCGGGGTTGCCGGGGAATGCTGCTGAAATACCGGGGCGTCCTACCGTGCCCAGAACCACAATCCCGGCCTGTAAGTGCTCGGCCAAACCAGGAATc                                                                                                                    >  1:1231278/1‑150 (MQ=255)
                                    ggTCAGTTTTAATGACCGGCAGGTCGCAGCGAAGTTGATCACCCCCCTGTTCCGCCGTGTTGCAGAGGAATGCTGCGAAAACCCCGGTGCGCCCTACCGGGCCCAGAACCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCa                                                                                                                   <  1:536004/150‑1 (MQ=255)
                                         gttttAAGGCCCAGCAGGTCGCAGCGAAGATGATCAATCCCCTGTCCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGACCCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCtctt                                                                                                             <  2:1263551/149‑1 (MQ=255)
                                         ggTTTAAGGACCAGCAGGTCGCAGCGAAGATGATCAATCCCCTGTCCCGCCGTTTTGCCGAGGAATGCTGCTAAAATCCCGGTGCGTCCTCCCGTGCCAAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCtctt                                                                                                             <  2:193904/151‑1 (MQ=255)
                                           ttaaaTGCCCAGCAGGTCGCAGCGAAGATGATCAACCCCCTGTTCCGCCGTGTTGCCGAGGAAGGCGGCTAAAATCCCGGTGCGTCCTCCCGTGCCCAGACCCAAAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCtctt                                                                                                             <  2:1222854/146‑1 (MQ=255)
                                           ttaaaTGACCAGCGGGTCGCAGCGAAGATCATCAACCCCCTTTTCCGCCGTTTCGCCGAGGAATGCTGCTAAAATACCGGTGCTTCCCACCGTGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCtcttc                                                                                                            <  1:619840/147‑1 (MQ=255)
                                            taaaTAACCAGCGGGTCGCGGCGAAAATAACCAATCCCCTGTTCCGCCGTGTTCCCGAGAAATGCTGCTGAAATCCCGGTGCGTCCTCCCGTGCCCAAACCCACATTCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCtcttct                                                                                                           <  2:1445716/148‑1 (MQ=255)
                                              aatgccgcgcagGTCGTGGCGAAAAGGATCAAGCGCCTGTCCCGCTGGTTTCCCGAGAAAGGCTGGGGAAATACGGGGGCGCCCTACCGGCCCAACACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTg                                                                                                          <  2:1142874/141‑1 (MQ=255)
                                              aaTGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGc                                                                                                        >  2:1279686/1‑151 (MQ=255)
                                               aaaccaagcagGCCGAACCCAATATGTTCATCCCCCTGTGCCGCCGTGTGGCCGAGGAATGCTGCAAAAATCCCGGTGCATCCTACCGGGCCCAGCCCAACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCtctt                                                                                                             <  3:26147/139‑1 (MQ=255)
                                                  aCCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGAc                                                                                                    >  2:387399/1‑151 (MQ=255)
                                                    cagcagGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCt                                                                                                  >  2:754725/1‑151 (MQ=255)
                                                       cagGTAGCAGCGAAGATGATCAATCACCTGATCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGACCCACAATCACCGCCTGTAAGCGATCCGCCAACTCAGGAATCACCTCTTCTGGCAGACCtctt                                                                                               >  1:1007814/1‑151 (MQ=255)
                                                        gggagacagaaaaaatgatCAGCCCCCTGTCCCGACATGTGGCGGAGGAATCCTCCGGAAACACCGGTCCCTCCCGCCGGACCCAGCCCCAAAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCCCCTCTTCTGGCAGACCttt                                                                                                <  1:982858/135‑1 (MQ=255)
                                                        agGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCttttt                                                                                              >  2:231986/1‑151 (MQ=255)
                                                         ggTCGCAGCGAAGATGATCAATCCCCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTAAAATCCCGGTGCGTCCTACCGTCCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCt                                                                                                  <  2:9556/146‑1 (MQ=255)
                                                         ggTCGCAGCGAAGATAATCAATCCCCTGTCCCGCCGTGTGGCCGAGGAATGCTGCTAAAATCCCGGTGCGTCCTACCTTGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCtttt                                                                                               <  3:263264/149‑1 (MQ=255)
                                                            cGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTAc                                                                                          >  2:191608/1‑151 (MQ=255)
                                                               aGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTg                                                                                       >  1:769940/1‑151 (MQ=255)
                                                               aGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTg                                                                                       >  2:1724091/1‑151 (MQ=255)
                                                               aGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTg                                                                                       >  1:1447083/1‑151 (MQ=255)
                                                                gCGAAGATGATCAATCCCCTGTCCCGCCGTGTTGCCGAGGAATGCTGCTAAAATCCCGGTGCGTCCTACCGTGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGt                                                                                        <  2:219353/149‑1 (MQ=255)
                                                                gCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTg                                                                                       >  4:35443/1‑150 (MQ=255)
                                                                gCGAAGATGATCAATCACCTGTCCCGCCGTGTGGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCAAAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTg                                                                                       <  2:491711/150‑1 (MQ=255)
                                                                   aaGATGATCAATCCCCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATCCCGGTGCGTCCTACCGTGCCCAGACCCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGc                                                                                      <  2:1211327/148‑1 (MQ=255)
                                                                   aaGATGATCAATCCCCTGTCCCGCCGTGTTGCCGAGGAATGCTGCTGAAATCCCGGTGCGTCCTACCGTGCCCAAACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTc                                                                                   <  1:144526/151‑1 (MQ=255)
                                                                   aaGATGATCAATCCCCTGTCCCGCCGTGTTGCCGAGAAATGCTGCTGAAATCCCGGTGCGTCCTACCGTGCCCAGAACCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTc                                                                                   <  1:1199345/151‑1 (MQ=255)
                                                                   aaGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTc                                                                                   >  1:1711659/1‑151 (MQ=255)
                                                                   aaGATGATCAACCCCCTGTCCCGCCGTGTTGCCGAGGAATGCTGCTAAAATCCCGGTGCGTCCTACCGTGCCAAAACCCAAATCCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGc                                                                                      <  2:1469647/148‑1 (MQ=255)
                                                                     gatgatAATTCACCTTTCCCGCCGTGTTGCCGAGGAATGCTGCTAAAATCCCGGTGCGTCCTCCCGTGCCCAGAACCACAAACCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCg                                                                                     <  2:1384137/147‑1 (MQ=255)
                                                                      atgatCAATCACCTGTTCCGCCGTTTTGCCGAGGAATGCTGCTAAAATCCCGGTGCGTCCTACCGTCCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCa                                                                                  <  2:1158384/149‑1 (MQ=255)
                                                                         accaatccCCTGTCCCGCCGTTTTCCCGAGGAATGCTGCTAAAATCCGGGTGCGCCCTACCGCCCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATg                                                                                <  1:365746/146‑1 (MQ=255)
                                                                            aacccccTTTTCCGCCGTGTTGCCGAGGAGTGCTGCTAAAATCCCGGTGCGCCCTCCCGTCCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGtttt                                                                            <  3:256791/144‑1 (MQ=255)
                                                                             atcaCCTGTTCCCCTGTTTTGCCGAGAAATGCTGCTGAAATCCCTGGGCGTCCTCGCGTCCCCAGATCCACATCCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGttt                                                                             <  3:306179/147‑1 (MQ=255)
                                                                                aCCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTa                                                                       >  1:530512/1‑150 (MQ=255)
                                                                                 ccTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATCCCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAAt                                                                     <  2:1251963/151‑1 (MQ=255)
                                                                                   tGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGc                                                                   >  2:1537987/1‑151 (MQ=255)
                                                                                     tcccgccgTTTTGCGGAGAAATGCTGCGAAAATCCCGGTGCCTCCAACCGTCCCCAAACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGtttt                                                                            <  4:596930/138‑1 (MQ=255)
                                                                                     tccccccgTTTGGCCGAGAAAGCCTGCTAAAATCCGGGGGCGTCCTCCCGTCCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGc                                                                   <  1:1704100/144‑1 (MQ=255)
                                                                                      cccgccgGGTTGCCAAGAAATGCTGCTAAAATCCCGGGGCTCCCTACCGTCCCAAGAACCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGc                                                                   <  4:332309/147‑1 (MQ=255)
                                                                                        cgccgTGTTGCCGAGAAATGCTGCTAAAATCCCGGTGCGCCCTACCGTGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAAt                                                              <  4:44324/151‑1 (MQ=255)
                                                                                           cgTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTc                                                           >  2:1166256/1‑151 (MQ=255)
                                                                                           cgTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTc                                                           >  2:738365/1‑151 (MQ=255)
                                                                                           cgTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTc                                                           >  2:1124944/1‑151 (MQ=255)
                                                                                            gtgtTGCCGAGAAATCCTGCTGAAATCCCGGTGCTTCCTCCCGTCCCAAGACCCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAAtt                                                             <  1:871011/148‑1 (MQ=255)
                                                                                             tgtTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCACCCGGGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTc                                                           <  2:274257/149‑1 (MQ=255)
                                                                                               ttGCCGAGGAATGCTGCGAAAATACCGGGGCGTCCTACCGTCCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCt                                                          <  2:1079/148‑1 (MQ=255)
                                                                                               ttGCCGAGGAAGGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAAACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTg                                                         <  2:765189/149‑1 (MQ=255)
                                                                                                  ccGGGGAATGCTGCAAAAATCCCGGTCCGTCCTACCGTGCCCAAAACCCCAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGc                                                     <  1:1537826/150‑1 (MQ=255)
                                                                                                  ccGAGGAATGCTGCTGAAATCCCGGTGCGCCCTACCGTGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTg                                                         <  1:1331569/146‑1 (MQ=255)
                                                                                                  ccGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGc                                                     >  1:301882/1‑150 (MQ=255)
                                                                                                    gAGGAATGCTGCTGAAATCCGGGTGCGTCCTACCGTGCCCAAACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGc                                                     <  1:1589077/148‑1 (MQ=255)
                                                                                                    gAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAgg                                                  >  2:765746/1‑151 (MQ=255)
                                                                                                     aGGAATGCTGCTGAAATCCCGGTGCGCCCTACCGTGCCCAGACCCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAgg                                                  <  1:1455578/150‑1 (MQ=255)
                                                                                                      ggAATGCTGCTAAAATCCCGGTGCGTCCTACCGTCCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAggg                                                 <  2:1102399/150‑1 (MQ=255)
                                                                                                      ggAATCCTGCTGAAATACGGGTGCGTCCTCCCGTGCCCAAAACCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGc                                                <  2:654583/151‑1 (MQ=255)
                                                                                                        aaTGCTGCTAAAATCCCGGGGCGTCCTACCGCCCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGc                                                <  2:1077501/149‑1 (MQ=255)
                                                                                                          tgctgcTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTc                                            >  1:847617/1‑151 (MQ=255)
                                                                                                             tgctgAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCAtt                                         >  1:1614565/1‑151 (MQ=255)
                                                                                                              gctaAAATCCCGGGGCGCCCTCCCGTGCCCATAACCCCAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCAt                                          <  1:513748/149‑1 (MQ=255)
                                                                                                                tgAACTCCCGGTGCGCCCTACCGCGCCTAGACCCACACTCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGTAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCAtt                                         <  1:1702313/148‑1 (MQ=255)
                                                                                                                  aaaTACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGcc                                      >  2:1623670/1‑149 (MQ=255)
                                                                                                                   aaTACCGGTGCGTCCTACCGTGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGc                                   <  1:1161928/151‑1 (MQ=255)
                                                                                                                       ccGGTGCGTCCTACCGTGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCaaa                                <  2:1029661/150‑1 (MQ=255)
                                                                                                                        cGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATg                              >  2:991531/1‑151 (MQ=255)
                                                                                                                          gTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGtt                            >  2:481618/1‑151 (MQ=255)
                                                                                                                              gTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGccc                        >  2:1120748/1‑151 (MQ=255)
                                                                                                                                ccTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCAc                      >  2:992851/1‑151 (MQ=255)
                                                                                                                                 ccaCCGTCCCAAAAACCATACCCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACg                     <  1:308587/149‑1 (MQ=255)
                                                                                                                                    ccGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACg                     <  2:1246016/148‑1 (MQ=255)
                                                                                                                                     cggtcccAAAACCCCAATTCCCCGCCTTTAGTTGCTCCGCCAAATCAGGAATCACCCTTTCTGGCAGACCTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTACATTGCCAGCAAATGTTGCCCACGaa                   <  2:1488053/145‑1 (MQ=255)
                                                                                                                                      gtccccacacccacaACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATgg                <  1:1008180/148‑1 (MQ=255)
                                                                                                                                       tGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGc               >  2:1244692/1‑151 (MQ=255)
                                                                                                                                              aaCCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGtt         >  2:1449581/1‑150 (MQ=255)
                                                                                                                                              aaCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGt          <  1:20207/149‑1 (MQ=255)
                                                                                                                                                 cacaATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGtt         <  1:324158/147‑1 (MQ=255)
                                                                                                                                                 cacaATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATaa     <  2:724262/151‑1 (MQ=255)
                                                                                                                                                  acaATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATaa     <  3:367203/150‑1 (MQ=255)
                                                                                                                                                    aaTCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACg  >  3:312196/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                    
CTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACG  >  NC_000913/1397680‑1397978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: