Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,508,6620TA41.1% 170.9 / 44.6 112E384D (GAA→GAT) yhfWphosphopentomutase‑related metalloenzyme superfamily protein
Reads supporting (aligned to +/- strand):  ref base T (20/46);  new base A (44/2);  total (64/48)
Fisher's exact test for biased strand distribution p-value = 3.58e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

CACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATCAC  >  NC_000913/3508513‑3508811
                                                                                                                                                       |                                                                                                                                                     
cACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAAtt                                                                                                                                                      <  1:751332/151‑1 (MQ=255)
  cGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTca                                                                                                                                                    <  1:113429/151‑1 (MQ=255)
     tgttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GG‑GGGAAAAATaaca                                                                                                                                                  >  4:428129/1‑150 (MQ=255)
     tgttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATcaca                                                                                                                                                  >  3:117251/1‑150 (MQ=255)
      gttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTcacaca                                                                                                                                                <  1:939303/151‑1 (MQ=255)
      gttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTcaca                                                                                                                                                  >  2:1246974/1‑149 (MQ=255)
           tAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GCGGGAAAAAttaaa                                                                                                                                                   >  4:488096/1‑141 (MQ=255)
            aaaGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGAGGGGC‑GG‑GGGAAAAATTACACACgg                                                                                                                                             >  1:969703/1‑148 (MQ=255)
             aaGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGc                                                                                                                                         <  2:381503/151‑1 (MQ=255)
              aGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATCacacac                                                                                                                                               >  2:650969/1‑144 (MQ=255)
                   gagagaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGA‑GC‑GGAAAAATTcacaccagctaagccccc                                                                                                                                    >  1:995451/1‑137 (MQ=255)
                    agagaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATTACAAACGGGAGCCCCcac                                                                                                                                   >  2:1120835/1‑150 (MQ=255)
                       gaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCa                                                                                                                               <  1:1302669/151‑1 (MQ=255)
                       gaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGCGG‑GC‑GGGAAAAATAACAACCGGGAGgcccccc                                                                                                                                   >  1:348417/1‑145 (MQ=255)
                       gaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGAGGCGG‑GC‑GGGAAAAATAAAACACgggcggccccccaac                                                                                                                                >  1:827955/1‑137 (MQ=255)
                         tCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGCGGGGC‑GC‑GGGAAAAAACACACACGGTAGccccccc                                                                                                                                   >  1:1103952/1‑143 (MQ=255)
                          cAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGCGC‑GC‑GGGAAAAATAAAAAACGGGAGCCCCCACATCa                                                                                                                               >  1:377438/1‑148 (MQ=255)
                           aaTATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGaaa                                                                                                                           <  4:159001/151‑1 (MQ=255)
                           aaTATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGCGTGGG‑GC‑GGGAAAAATAAAACCCGGGAcccccccccctct                                                                                                                              >  1:36445/1‑141 (MQ=255)
                            atatGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAg                                                                                                                          <  1:1313776/151‑1 (MQ=255)
                             tatGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATTACAAACCGGAGCCCCCCCATCAGaa                                                                                                                            >  1:825564/1‑148 (MQ=255)
                             tatGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGCGGGGC‑GC‑GGGAAAAAATACACACCGGAGACCCCCCATccaaaa                                                                                                                           >  1:1228760/1‑144 (MQ=255)
                              atGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAATTCACACACCGGACCCCCCACCTccaaaaac                                                                                                                         >  1:1294478/1‑143 (MQ=255)
                                   gATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCgtggtg                                                                                                                   <  3:173558/151‑1 (MQ=255)
                                     tGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATCACACACGGTAGCCCCCCCCTCAGAAAGCgggg                                                                                                                     >  2:1651423/1‑147 (MQ=255)
                                      gCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATTACACACGGTAGCCCCCCCATCAGAAAACGTGGTgcg                                                                                                                 >  3:563510/1‑150 (MQ=255)
                                      gCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATCACACACGGGAGCCCCCCCATCAGAAAACGGGGGgcg                                                                                                                 >  4:453602/1‑150 (MQ=255)
                                         cATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATTACAAACCGGAGACCCCCCATCAGAAAACgtgggggg                                                                                                                 >  2:152361/1‑143 (MQ=255)
                                          aTGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGG‑GG‑GGGAAAAATTAAAAACCGGAGCCCCCACATCAGaaaagggggtggg                                                                                                                 >  2:614220/1‑136 (MQ=255)
                                            aggCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATTACACACCGGAGCCCCCCCATCCGAAAGAGGGGGGGGCACAcc                                                                                                           >  1:918273/2‑150 (MQ=255)
                                             ggCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGa                                                                                                         <  3:92143/151‑1 (MQ=255)
                                                gAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGGAAAATCACACACGGGAGCCCCCCCATCAGAAAAAGGGGTGCGCACACCGa                                                                                                         >  1:1494621/1‑148 (MQ=255)
                                                gAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCTCCCACATCAGAAAGCGTGGTGCGCACACCGAGCt                                                                                                      <  2:1646047/151‑1 (MQ=255)
                                                 aGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATTACACACGGGAACCCCCACACCCGAAAGCGGGGGGCGCACACCCAGATg                                                                                                     >  1:937094/1‑151 (MQ=255)
                                                 aGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTg                                                                                                     <  2:446910/151‑1 (MQ=255)
                                                   ggTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCg                                                                                                   <  3:413185/151‑1 (MQ=255)
                                                     tgtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATTACACACGGTAGCCCCCCCATCAGAAAGCGGGGGGGGCCAACCCAGCcgcg                                                                                                   >  3:205723/1‑149 (MQ=255)
                                                     tgtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAAATCACACCCGGGAGCCCCCCCATCAGAAAACGTGGGGCGCACACCGAGCTg                                                                                                     >  2:1630068/1‑147 (MQ=255)
                                                     tgtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGATGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGt                                                                                                  >  2:458466/1‑150 (MQ=255)
                                                      gtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAg                                                                                                <  2:852452/151‑1 (MQ=255)
                                                        aaCCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GG‑GGGAAAAATCACAAACGGTAGCCCCCCCACCAGAAAGCGGGGGGGGCACACCGAGCTGCGGAg                                                                                                >  1:259629/1‑149 (MQ=255)
                                                        aaCCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAga                                                                                               <  1:1503282/150‑2 (MQ=255)
                                                         aCCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGa                                                                                             <  2:543481/151‑1 (MQ=255)
                                                         aCCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGa                                                                                             <  2:386357/151‑1 (MQ=255)
                                                         aCCCGTCGGATCAAAACTCATAGGGTGTCTCATGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGAGGCGGCGG‑GC‑GGGAAAAAAAAAACACGGGAGGCACCACCACAAAAAAAGTGGGGGGGACAcccgaccgagtag                                                                                                >  1:1299212/1‑137 (MQ=255)
                                                          cccGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATCACACACGGGAGCCCCCCCATCAGAAAACGGGGGGCGCCCACCCAGCTGCGTAGCggt                                                                                            >  4:504863/1‑149 (MQ=255)
                                                           ccGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGat                                                                                            <  3:301668/150‑1 (MQ=255)
                                                            cGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATCAAACACGGTAACCCCCCCCTCCAAAAACGGGGGGCGCCCACCGAGCCGCGTCGCGatc                                                                                           >  1:1673544/1‑150 (MQ=255)
                                                            cGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGatca                                                                                          <  1:134819/151‑1 (MQ=255)
                                                             gTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGAGGCGC‑GC‑GGGAAAAATTACACACGGTAGCCCCCACAACAGAAAACGGGGTGCGCACACCCAGCTGCGGAGCGgtca                                                                                          >  2:76164/1‑150 (MQ=255)
                                                               cGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATAACACACGGGAACCCCCACATCAGAAAAAGGGGGGGGCACACCGAGCTGCGGAGCGATCATcc                                                                                       >  1:189808/1‑151 (MQ=255)
                                                               cGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATcc                                                                                       <  3:60337/151‑1 (MQ=255)
                                                               aggATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTTGCGC‑GC‑GGGAAAATTCACACACGGGAGCCCCCACATCAGAAAACGTGGGGCGCACACCGAGCTGCGTAGCGATCATcc                                                                                       >  2:354824/2‑151 (MQ=255)
                                                                  aTCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCtt                                                                                    <  2:603013/151‑1 (MQ=255)
                                                                     aaaaCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGc                                                                                  <  2:703300/150‑1 (MQ=255)
                                                                       aaCTCATAGGGTGTCTCCTGCAAAGCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGa                                                                               <  2:960035/151‑1 (MQ=255)
                                                                            aTAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATaaa                                                                           <  2:83080/150‑1 (MQ=255)
                                                                            aTAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAAc                                                                          <  1:1019157/151‑1 (MQ=255)
                                                                              aGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCAAACAAGGGTGGCCCCCCACCAAGAAAAGTGGGGCGCCCACCGAGCCGCGGAACGATCATCCCCTTCTGGTaaa                                                                           >  2:1301096/1‑148 (MQ=255)
                                                                                ggTGTCTCCTGCAAACCGGAGGTAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑AC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCa                                                                        <  2:1138002/149‑1 (MQ=255)
                                                                                  tgtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATTAAAAAAGGTAGCCCCCACACCAGAAAACGTGGTGGGCACACCCCGCTGCGGAGCGATCCTCCCTTGCTGATAAACCaac                                                                      >  4:305765/1‑147 (MQ=255)
                                                                                   gtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCAc                                                                    <  1:624160/150‑1 (MQ=255)
                                                                                   gtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACt                                                                   <  1:1271464/151‑1 (MQ=255)
                                                                                     ctcCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑CC‑GGAAAAATTCAAACACGGTAGCCCCCCCATCAGAAAAAGGGGGGCGGACACCCAGCTGCGCAGAGAACATCCCCTTCTGGTAAAACAGCACttg                                                                 >  1:1210563/1‑149 (MQ=255)
                                                                                       ccTGAAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAAATCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTCGCTGATAAACAAGCACTGGca                                                               <  1:665597/151‑1 (MQ=255)
                                                                                        cTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGG‑GC‑GGGAAAAATCAAAAACAGGATGCCCCACAACCAAAAGAGTGGTGCGCACACCGCGCTGCGTAGCGATCATCCCCTTCTGATAAAACAACACTGGcac                                                              >  1:1625120/1‑151 (MQ=255)
                                                                                              aCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTc                                                        <  4:189614/151‑1 (MQ=255)
                                                                                                 ggagggagGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTcgcg                                                     <  2:783287/151‑1 (MQ=255)
                                                                                                  gagggaggAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATCAAACACGGTAGCCCCCCCATCAGAAAACGGGGGGCGCCCACCCAGCCGCGTAGCGATCATCCCCTGCTGATAAAACAACACCGGGACCACCTCCCgg                                                    >  2:1336959/1‑151 (MQ=255)
                                                                                                   agggaggAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAATTCACACACCGTAGCCCCCCCATCAGAAAACGGGGGGCGCACACCCAGCTGCGTAGCGATCATCCCTTTCTGATAAAACAGCACTGGCACCACCTCCCggg                                                   >  2:1515346/1‑151 (MQ=255)
                                                                                                   agggaggAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAAATCACACACCGTAGCCCCCACATCAGAAAGCGTGGGGCGCACAACGAGCTGCGTAACGATCATCCCTTGCTGGTAAACCAGCACTGGCACCACCTCGCggg                                                   >  2:1435085/1‑151 (MQ=255)
                                                                                                   agggaggAAAGAAAAGAGCGACCATTTTGTGGCGGCGGGGC‑GC‑GGGAAAAATCACAAACGGGAGCCCCCCCCACAGAAAAGGTGGGGGGCGCAACCAGCCGCGAGGTGATCATCCCCTGCTGTTAAAACAGCCCTGGACCCACTTAGCggg                                                   >  1:1140759/1‑151 (MQ=255)
                                                                                                    gggaggAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATCACACACGGGAGCCCCCACCTCAGAAAGCGTGGTGCGCACACCGAGCCGCGGAGCGATCATCCCCTGCTGATAAAACAGCACTGGCACCACCTCGCgggg                                                  >  2:351149/1‑150 (MQ=255)
                                                                                                     ggaggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATTACACACGGGAACCCCCCCATCAGAAAACGGGGGGCGCACAACGAGGCGCGGAGCGATCATCCCCTGCTGATAAAACAACACCGGCACCCCCTCGCggg                                                   >  2:338173/1‑149 (MQ=255)
                                                                                                       aggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGTGCGGG‑GGAAAAATTAAAAAAGGGAAGCCCCC‑CCTCCAAAAACGGGGGGGGGGCAACGAGCCGCGGAAGGATCATCCCTTTCTTATAAAACAGAACTGGcaccaccttccggg                                                   >  4:360992/1‑139 (MQ=255)
                                                                                                        ggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATTACACACGGTAACCCCCCCAACAGAAAAAGGGGGGCGCACACCGAGCTGCGTAGCGATCATCCCCTGCTGATAAAACAGCACTGGCACCACTTCGCGGGGAtgg                                              >  3:367641/1‑151 (MQ=255)
                                                                                                          aaagaaaagaGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATTACACACGGGAGCCCCCCCATCAGAAAACGTGGGGGGGACACCCAGCCGCGGAGCGATCATCCCCTGCTGATAAAACAGCACCGGCCCCCCCTCGCgggggtgg                                              >  2:1639839/1‑144 (MQ=255)
                                                                                                                aagaGCGTCCATTTTGTGGCGGTGACGC‑GC‑GGAAAAATTCACAGACGGTAGCCCCCACAACAGATAGCGTGGTGCGTACACCGAGCTGCGTAGCGGTCATCCCTTGCTGATAAACCAGCCCTGGCACCACTTCGCGGGTATGGTGGCtgtg                                      <  1:1324245/151‑1 (MQ=255)
                                                                                                                   agCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAATTCACACACGGTAGCCCCCACATCAGAAAACGGGGGGGGCACACCCAGCTGCGTAGCGATCATCCCCTTCTGATAAAACAGCACCGGCACCACCTCGCGGGGATGGGGGGTGTGAcc                                   >  2:919027/1‑151 (MQ=255)
                                                                                                                        ccATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATgg                              <  2:21683/151‑1 (MQ=255)
                                                                                                                             ttGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCgg                          <  1:809487/150‑1 (MQ=255)
                                                                                                                                 ggcggtggcgC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGt                     <  2:801909/151‑1 (MQ=255)
                                                                                                                                    ggtggcgC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGTTCGGATCGTTg                   >  1:1228002/1‑150 (MQ=255)
                                                                                                                                    ggtggcgC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGc                  >  1:505075/1‑151 (MQ=255)
                                                                                                                                      tggcgC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGGATGGTGGCTGTGACCAATGGTCGGATCGTTGcc                 >  2:141287/1‑150 (MQ=255)
                                                                                                                                       ggcgC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGt               <  3:567000/151‑1 (MQ=255)
                                                                                                                                       ggcgC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGt               <  2:1294628/151‑1 (MQ=255)
                                                                                                                                        gcgc‑gc‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTg              <  1:1090072/151‑1 (MQ=255)
                                                                                                                                        gcgc‑gc‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTg              <  2:805677/151‑1 (MQ=255)
                                                                                                                                        gcgc‑gc‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTg              >  2:63736/1‑151 (MQ=255)
                                                                                                                                           c‑gc‑gGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATc           <  2:1624646/151‑1 (MQ=255)
                                                                                                                                                ggAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCg         >  4:467718/1‑150 (MQ=255)
                                                                                                                                                 gAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGcc       >  2:589534/1‑151 (MQ=255)
                                                                                                                                                 gAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGcc       <  1:194930/151‑1 (MQ=255)
                                                                                                                                                  aaaaaTTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAACCCAGCACTGGCACCTCTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCa      <  1:346373/151‑1 (MQ=255)
                                                                                                                                                  aaaaaTTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCa      <  2:636308/151‑1 (MQ=255)
                                                                                                                                                   aaaaTTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCAt     <  1:838134/151‑1 (MQ=255)
                                                                                                                                                    aaaTTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATc    >  2:173088/1‑151 (MQ=255)
                                                                                                                                                     aaTTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATc    >  1:4337/1‑150 (MQ=255)
                                                                                                                                                      aTTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATCAc  <  2:810068/151‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
CACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATCAC  >  NC_000913/3508513‑3508811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑