Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,313,236 T→C intergenic (‑58/+106) rbfA ← / ← infB 30s ribosome binding factor/translation initiation factor IF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,313,2360TC99.3% 546.1 / NA 145intergenic (‑58/+106)rbfA/infB30s ribosome binding factor/translation initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (77/67);  minor base A (1/0);  total (78/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

GAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCGATGATTTCGAATACTTC  >  NC_000913/3313088‑3313383
                                                                                                                                                    |                                                                                                                                                   
 aGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCgg                                                                                                                                                   >  1:883544/1‑150 (MQ=255)
 aGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGa                                                                                                                                                  <  1:1654292/151‑1 (MQ=255)
  ggATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGaa                                                                                                                                                 <  2:150418/151‑1 (MQ=255)
      cTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGc                                                                                                                                             >  4:397679/1‑151 (MQ=255)
        ttAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAg                                                                                                                                                <  2:1664577/146‑1 (MQ=255)
        ttAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAg                                                                                                                                                <  1:880112/146‑1 (MQ=255)
         tAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGc                                                                                                                                             <  2:480907/148‑1 (MQ=255)
         tAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTg                                                                                                                                           <  1:1000876/150‑1 (MQ=255)
         tAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTg                                                                                                                                           <  2:1073666/150‑1 (MQ=255)
         tAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTg                                                                                                                                           <  2:529443/150‑1 (MQ=255)
            tttCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTc                                                                                                                                        >  2:1197957/1‑150 (MQ=255)
             ttCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCAc                                                                                                                                      >  1:319400/1‑151 (MQ=255)
              tCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACg                                                                                                                                     >  4:558501/1‑151 (MQ=255)
               cACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACg                                                                                                                                     <  2:1627216/150‑1 (MQ=255)
                aCGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGtt                                                                                                                                   >  1:929770/1‑151 (MQ=255)
                  gCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCAGAAGGGCTGTTCACGTTg                                                                                                                                  >  2:1285808/1‑150 (MQ=255)
                    tGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGAcc                                                                                                                               >  2:1177001/1‑151 (MQ=255)
                      cAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTg                                                                                                                             <  2:755381/151‑1 (MQ=255)
                         gATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGAt                                                                                                                           >  2:985107/1‑150 (MQ=255)
                         gATGAGAGCCATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGTCCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATa                                                                                                                          <  1:1596156/151‑1 (MQ=255)
                           tGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATa                                                                                                                          >  3:571066/1‑149 (MQ=255)
                                  gATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGAcgcg                                                                                                                   >  1:1262100/1‑149 (MQ=255)
                                  gATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGcc                                                                                                                 >  2:919754/1‑151 (MQ=255)
                                   aTCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGcc                                                                                                                 >  2:1311262/1‑150 (MQ=255)
                                     ctctTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGc                                                                                                              >  2:346572/1‑151 (MQ=255)
                                       ctTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGt                                                                                                            >  3:124656/1‑151 (MQ=255)
                                       ctTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGt                                                                                                            >  2:389984/1‑151 (MQ=255)
                                             gCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACAt                                                                                                       <  1:1439058/150‑1 (MQ=255)
                                               aTTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATc                                                                                                      >  4:560738/1‑149 (MQ=255)
                                               aTTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAg                                                                                                    <  2:1541498/151‑1 (MQ=255)
                                               aTTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAg                                                                                                    <  2:810606/151‑1 (MQ=255)
                                                  tCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGc                                                                                                  >  1:729762/1‑150 (MQ=255)
                                                  tCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGc                                                                                                  <  1:1121343/150‑1 (MQ=255)
                                                     tGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATc                                                                                              >  2:1065559/1‑151 (MQ=255)
                                                      gcgcTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATcc                                                                                             >  1:388844/1‑151 (MQ=255)
                                                         cTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCAt                                                                                           >  1:1137371/1‑150 (MQ=255)
                                                           aCGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATg                                                                                          <  1:207773/149‑1 (MQ=255)
                                                           aCGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGcc                                                                                        >  4:113494/1‑151 (MQ=255)
                                                                cTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGAt                                                                                    <  2:894658/150‑1 (MQ=255)
                                                                  gCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATg                                                                                   <  1:913700/149‑1 (MQ=255)
                                                                  gCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATg                                                                                   <  4:228135/149‑1 (MQ=255)
                                                                  gCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGc                                                                                  >  2:672114/1‑150 (MQ=255)
                                                                  gCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGc                                                                                  >  1:362978/1‑150 (MQ=255)
                                                                   cGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAg                                                                                <  3:541986/151‑1 (MQ=255)
                                                                      gCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGt                                                                             >  4:272565/1‑151 (MQ=255)
                                                                       cGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGt                                                                             >  2:1464108/1‑150 (MQ=255)
                                                                          ccAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAAc                                                                         <  3:166685/151‑1 (MQ=255)
                                                                           cAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACg                                                                        <  1:1005117/151‑1 (MQ=255)
                                                                              aTTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCt                                                                     >  4:401627/1‑151 (MQ=255)
                                                                                    tCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATccc                                                               >  1:34447/1‑151 (MQ=255)
                                                                                     cGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCg                                                              <  2:967012/151‑1 (MQ=255)
                                                                                     cGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCg                                                              <  1:1001795/151‑1 (MQ=255)
                                                                                     cGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCg                                                              <  1:669954/151‑1 (MQ=255)
                                                                                       ccATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGc                                                             >  2:74274/1‑150 (MQ=255)
                                                                                         ataataAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATg                                                          <  2:1387305/151‑1 (MQ=255)
                                                                                         ataataAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATg                                                          >  2:1401435/1‑151 (MQ=255)
                                                                                         ataataAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATg                                                          <  2:736702/151‑1 (MQ=255)
                                                                                         ataataAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATg                                                          <  2:1142846/151‑1 (MQ=255)
                                                                                           aataaATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGa                                                        <  1:834674/151‑1 (MQ=255)
                                                                                              aaaTTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAAcc                                                     <  2:380566/151‑1 (MQ=255)
                                                                                                aTTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCt                                                   >  1:1519700/1‑151 (MQ=255)
                                                                                                aTTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCt                                                   >  1:409353/1‑151 (MQ=255)
                                                                                                 ttCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCt                                                   >  1:1019649/1‑150 (MQ=255)
                                                                                                 ttCTCATGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGAGGAAGGGCTGTTCACGTTGACCTGATAAGATGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCt                                                   >  1:1029974/1‑150 (MQ=255)
                                                                                                  tctcCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTa                                                  <  1:1183479/150‑1 (MQ=255)
                                                                                                     ccTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTaaaa                                               >  1:166416/1‑150 (MQ=255)
                                                                                                     ccTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTaaa                                                <  1:48273/149‑1 (MQ=255)
                                                                                                     ccTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTaa                                                 <  3:225684/148‑1 (MQ=255)
                                                                                                     ccTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTaa                                                 <  1:380170/148‑1 (MQ=255)
                                                                                                      cTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAAc                                             >  1:481633/1‑151 (MQ=255)
                                                                                                        gACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCt                                           <  2:1079200/151‑1 (MQ=255)
                                                                                                            aaaaaGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAg                                       <  2:1706236/151‑1 (MQ=255)
                                                                                                             aaaaGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGc                                      <  2:1231342/151‑1 (MQ=255)
                                                                                                                aGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAAt                                   >  4:446073/1‑151 (MQ=255)
                                                                                                                  gggCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATgg                                 >  4:447056/1‑151 (MQ=255)
                                                                                                                   ggCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATgg                                 >  3:178060/1‑150 (MQ=255)
                                                                                                                    gCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGt                                >  2:41854/1‑150 (MQ=255)
                                                                                                                       gTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTAc                              <  4:146528/149‑1 (MQ=255)
                                                                                                                       gTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGt                            >  3:60019/1‑151 (MQ=255)
                                                                                                                         tAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGtt                           <  1:449461/150‑1 (MQ=255)
                                                                                                                            ccccTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGAt                       >  1:777214/1‑151 (MQ=255)
                                                                                                                            ccccTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGAt                       >  3:379321/1‑151 (MQ=255)
                                                                                                                            ccccTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGAt                       <  2:1334846/151‑1 (MQ=255)
                                                                                                                              ccTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGAtct                     >  2:849703/1‑151 (MQ=255)
                                                                                                                              ccTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGAtct                     <  4:140251/151‑1 (MQ=255)
                                                                                                                                tttttAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCg                   >  3:437775/1‑151 (MQ=255)
                                                                                                                                  tttAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCgat                 >  2:1382301/1‑151 (MQ=255)
                                                                                                                                    tAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCgatg                >  3:155611/1‑150 (MQ=255)
                                                                                                                                    tAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCg                   >  1:1095840/1‑147 (MQ=255)
                                                                                                                                     aaaaTTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCgatgat              <  2:1262246/151‑1 (MQ=255)
                                                                                                                                       aattaattTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCGATGAttt            >  1:1184401/1‑151 (MQ=255)
                                                                                                                                        attaattTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCGATGATTTc           <  3:259331/151‑1 (MQ=255)
                                                                                                                                             tttCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCGATGATTTCGAAt       >  2:310292/1‑150 (MQ=255)
                                                                                                                                              ttCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCGATGATTTCGAATa      <  3:113499/150‑1 (MQ=255)
                                                                                                                                              ttCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCGATGATTTCGAATAc     >  4:254080/1‑151 (MQ=255)
                                                                                                                                               tCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCGATGATTTCGAATACt    >  2:910425/1‑151 (MQ=255)
                                                                                                                                                 aGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCGATGATTTCGAATACTTc  >  1:1382768/1‑151 (MQ=255)
                                                                                                                                                 aGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCGATGATTTCGAATACTTc  >  1:191905/1‑151 (MQ=255)
                                                                                                                                                 aGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCGATGATTTCGAATACTTc  >  1:10611/1‑151 (MQ=255)
                                                                                                                                                 aGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCGATGATTTCGAATACTTc  >  1:836578/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCGATGATTTCGAATACTTC  >  NC_000913/3313088‑3313383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: